Phosphorylation of the Cav3.2 T-type calcium channel directly regulates its gating properties.

abstract：:The radial distributions of the Calpha and side-chain atoms in a sample of 13 native proteins have been examined. It is found that there are substantial differences in behavior between different atoms of the same amino acid. In particular, the Calpha atoms of polar residues show no particular preference for being far ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rackovsky S,Scheraga HA

更新日期：1977-12-01 00:00:00

abstract：:Methanogenic archaea are major contributors to the global carbon cycle and were long thought to belong exclusively to the euryarchaeal phylum. Discovery of the methanogenesis gene cluster methyl-coenzyme M reductase (Mcr) in the Bathyarchaeota, and thereafter the Verstraetearchaeota, led to a paradigm shift, pushing b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berghuis BA,Yu FB,Schulz F,Blainey PC,Woyke T,Quake SR

更新日期：2019-03-12 00:00:00

abstract：:IFN-gamma treatment of cells leads to tyrosine phosphorylation of signal transducer and activator of transcription (STAT) 1 followed by dimerization through a reciprocal Src homology 2-phosphotyrosine interaction near the -COOH end of each monomer, forming a parallel structure that accumulates in the nucleus to drive ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhong M,Henriksen MA,Takeuchi K,Schaefer O,Liu B,ten Hoeve J,Ren Z,Mao X,Chen X,Shuai K,Darnell JE Jr

更新日期：2005-03-15 00:00:00

abstract：:We consider the sequential estimation of p, the probability of success in an infinite sequence of Bernoulli trials, when the loss incurred in stopping after n trials and estimating p by some function delta(n) of the first n outcomes is taken to be [Formula: see text] The loss due to error of estimation is thus the sym...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cabilio P,Robbins H

更新日期：1975-01-01 00:00:00

abstract：:KCNE β-subunits assemble with and modulate the properties of voltage-gated K+ channels. In the heart, KCNE1 associates with the α-subunit KCNQ1 to generate the slowly activating, voltage-dependent potassium current (IKs) in the heart that controls the repolarization phase of cardiac action potentials. By contrast, in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barro-Soria R,Ramentol R,Liin SI,Perez ME,Kass RS,Larsson HP

更新日期：2017-08-29 00:00:00

abstract：:The corticotropin-releasing factor (CRF) ligand family has diverse effects on the CNS, including the modulation of the stress response. The ligands' effects are mediated by binding to CRF G protein-coupled receptors. We have determined the 3D NMR structure of the N-terminal extracellular domain (ECD1) of the mouse CRF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grace CR,Perrin MH,DiGruccio MR,Miller CL,Rivier JE,Vale WW,Riek R

更新日期：2004-08-31 00:00:00

abstract：:To improve the usefulness of in vivo mode for the investigation of the pathophysiology of human immunodeficiency virus (HIV) infection, we modified the construction of SCID mice implanted with human fetal thymus and liver (thy/liv-SCID-hu mice) so that the peripheral blood of the mice contained significant numbers of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kollmann TR,Pettoello-Mantovani M,Katopodis NF,Hachamovitch M,Rubinstein A,Kim A,Goldstein H

更新日期：1996-04-02 00:00:00

abstract：:The Ly-6 locus encodes several cell surface proteins whose functions are unknown. Although it is hypothesized that these proteins may be receptors, there is no direct evidence that they bind a ligand. Herein we present evidence that Ly-6A.2, a Ly-6 protein expressed on T lymphocytes, binds a ligand expressed on normal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bamezai A,Rock KL

更新日期：1995-05-09 00:00:00

abstract：:Although tumor necrosis factor (TNF) and interleukin 1 (IL-1) affect many cell functions, the molecular mechanisms of TNF and IL-1 action are not understood. Our present study shows that exposure of human FS-4 fibroblasts to TNF or IL-1 caused a rapid accumulation of intracellular cAMP and an increase in protein kinas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang YH,Lin JX,Yip YK,Vilcek J

更新日期：1988-09-01 00:00:00

abstract：:Surface functionalization of semiconductors has been the backbone of the newest developments in microelectronics, energy conversion, sensing device design, and many other fields of science and technology. Over a decade ago, the notion of viewing the surface itself as a chemical reagent in surface reactions was introdu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bent SF,Kachian JS,Rodríguez-Reyes JC,Teplyakov AV

更新日期：2011-01-18 00:00:00

abstract：:Beetle luciferases are thought to have evolved from fatty acyl-CoA synthetases present in all insects. Both classes of enzymes activate fatty acids with ATP to form acyl-adenylate intermediates, but only luciferases can activate and oxidize d-luciferin to emit light. Here we show that the Drosophila fatty acyl-CoA syn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mofford DM,Reddy GR,Miller SC

更新日期：2014-03-25 00:00:00

abstract：:The Millennium Ecosystem Assessment (MA) introduced a new framework for analyzing social-ecological systems that has had wide influence in the policy and scientific communities. Studies after the MA are taking up new challenges in the basic science needed to assess, project, and manage flows of ecosystem services and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carpenter SR,Mooney HA,Agard J,Capistrano D,Defries RS,Díaz S,Dietz T,Duraiappah AK,Oteng-Yeboah A,Pereira HM,Perrings C,Reid WV,Sarukhan J,Scholes RJ,Whyte A

更新日期：2009-02-03 00:00:00

abstract：:Poliovirus RNA polymerase requires a host factor to initiate RNA synthesis in vitro. The host factor was previously purified to near homogeneity from HeLa cells but was not assigned an enzymatic activity. This report describes the purification of a terminal uridylyltransferase that can act as host factor. By all crite...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Andrews NC,Baltimore D

更新日期：1986-01-01 00:00:00

abstract：:Transient expression of either alpha or alpha + beta subunits of the high-conductance Ca2+-activated K+ (maxi-K) channel has been achieved in COS-1 cells. Expression has been studied using charybdotoxin (ChTX), a peptidyl inhibitor that binds in the pore on the alpha subunit. Although some properties of monoiodotyrosi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hanner M,Schmalhofer WA,Munujos P,Knaus HG,Kaczorowski GJ,Garcia ML

更新日期：1997-04-01 00:00:00

abstract：:We have recently established that several mRNAs encode the PR264/SC35 splicing factor and that their expression is related to that of c-Myb in human hematopoietic cells. We now report that the various PR264 mRNAs whose expression is transactivated by c-Myb proteins encode an identical PR264/SC35 polypeptide. These mRN...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sureau A,Perbal B

更新日期：1994-02-01 00:00:00

abstract：:Humans respond to foreign antigen by generating plasma Abs and memory B cells (MBCs). The Ab response then declines, sometimes to below the limit of detection. In contrast, MBCs are generally thought to be long-lived. We tested and compared Plasmodium falciparum (Pf)-specific Ab and MBC responses in two populations of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ndungu FM,Olotu A,Mwacharo J,Nyonda M,Apfeld J,Mramba LK,Fegan GW,Bejon P,Marsh K

更新日期：2012-05-22 00:00:00

abstract：:We have developed a high-throughput approach to the labor-intensive problems of DNA cyclization, which we use to characterize DNA curvature and mechanical properties. The method includes a combinatorial approach to make the DNA constructs needed and automated real-time measurement of the kinetics using fluorescence. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Crothers DM

更新日期：2003-03-18 00:00:00

abstract：:After more than a decade of experiments generating and studying the physics of quantized vortices in atomic gas Bose-Einstein condensates, research is beginning to focus on the roles of vortices in quantum turbulence, as well as other measures of quantum turbulence in atomic condensates. Such research directions have ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White AC,Anderson BP,Bagnato VS

更新日期：2014-03-25 00:00:00

abstract：:Infection of newborn rats with Borne disease virus (BDV) results in selective degeneration of granule cell neurons of the dentate gyrus (DG). To study cellular countermechanisms that might prevent this pathology, we screened for rat strains resistant to this BDV-induced neuronal degeneration. To this end, we infected ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu YJ,Schulz H,Lin CC,Saar K,Patone G,Fischer H,Hübner N,Heimrich B,Schwemmle M

更新日期：2013-01-29 00:00:00

abstract：:The B-cell antigen receptor is composed of membrane immunoglobulin sheathed by an alpha/beta heterodimer. The complex is noncovalently associated with protein kinase activity, and crosslinking of the receptor leads to capping and transmembrane signaling. Here we show that the sheath is not necessary either for this ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams GT,Peaker CJ,Patel KJ,Neuberger MS

更新日期：1994-01-18 00:00:00

abstract：:The modern concept of neurogenesis in the adult brain is predicated on the premise that multipotent glial cells give rise to new neurons throughout life. Although extensive evidence exists indicating that this is the case, the transition from glial to neuronal phenotype remains poorly understood. A unique monolayer ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Scheffler B,Walton NM,Lin DD,Goetz AK,Enikolopov G,Roper SN,Steindler DA

更新日期：2005-06-28 00:00:00

abstract：:To prevent the spread of coronavirus disease 2019 (COVID-19), some types of public spaces have been shut down while others remain open. These decisions constitute a judgment about the relative danger and benefits of those locations. Using mobility data from a large sample of smartphones, nationally representative cons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benzell SG,Collis A,Nicolaides C

更新日期：2020-06-30 00:00:00

abstract：:We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G+C content of 70.8% and two plasmids of 184,027 (pNF1) and 87,093 (pNF2) bp with average G+C ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishikawa J,Yamashita A,Mikami Y,Hoshino Y,Kurita H,Hotta K,Shiba T,Hattori M

更新日期：2004-10-12 00:00:00

abstract：:Although most experimental and theoretical studies of protein folding involve proteins in vitro, the effects of spatial confinement may complicate protein folding in vivo. In this study, we examine the folding dynamics of villin (a small fast folding protein) with explicit solvent confined to an inert nanopore. We hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lucent D,Vishal V,Pande VS

更新日期：2007-06-19 00:00:00

abstract：:The perforant path (PP) undergoes synaptic changes in the course of aging and dementia. Previous studies attempting to assess the integrity of the PP in humans using diffusion tensor imaging (DTI) were limited by low resolution and the inability to identify PP fibers specifically. Here we present an application of DTI...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yassa MA,Muftuler LT,Stark CE

更新日期：2010-07-13 00:00:00

abstract：:While the concept of intercellular mechanical communication has been revealed, the mechanistic insights have been poorly evidenced in the context of myofibroblast-fibroblast interaction during fibrosis expansion. Here we report and systematically investigate the mechanical force-mediated myofibroblast-fibroblast cross...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu L,Yu H,Zhao H,Wu Z,Long Y,Zhang J,Yan X,You Z,Zhou L,Xia T,Shi Y,Xiao B,Wang Y,Huang C,Du Y

更新日期：2020-05-19 00:00:00

abstract：:Primary cultures of differentiated liver cells from fetal rats respond to serum from partially hepatectomized rats by an increased uptake of [(3)H]thymidine and [(3)H]leucine as compared with cultures incubated with normal rat serum. These findings are discussed in relation to the mechanism of induction of DNA synthes...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paul D,Leffert H,Sato G,Holley RW

更新日期：1972-02-01 00:00:00

abstract：:Antibody modulation of T-cell coinhibitory (e.g., CTLA-4) or costimulatory (e.g., 4-1BB) receptors promotes clinical responses to a variety of cancers. Therapeutic cancer vaccination, in contrast, has produced limited clinical benefit and no curative therapies. The E6 and E7 oncoproteins of human papilloma virus (HPV)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bartkowiak T,Singh S,Yang G,Galvan G,Haria D,Ai M,Allison JP,Sastry KJ,Curran MA

更新日期：2015-09-22 00:00:00

abstract：:Sympathetic ganglia and some peripheral tissues of adult guinea pig and cat were analyzed by the indirect immunofluorescence technique with antisera to catecholamine-synthesizing enzymes and some peptides. In the guinea pig, noradrenergic neurons could be subdivided into three populations containing respectively (i) s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lundberg JM,Hökfelt T,Anggård A,Terenius L,Elde R,Markey K,Goldstein M,Kimmel J

更新日期：1982-02-01 00:00:00

abstract：:The question of what is a perceptual object is one of the most central and also controversial issues in cognitive science. According to the topological approach to perceptual organization, the core intuitive notion of an object--the holistic identity preserved over shape-changing transformations--may be characterized ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou K,Luo H,Zhou T,Zhuo Y,Chen L

更新日期：2010-12-14 00:00:00