Abstract:
:The modern concept of neurogenesis in the adult brain is predicated on the premise that multipotent glial cells give rise to new neurons throughout life. Although extensive evidence exists indicating that this is the case, the transition from glial to neuronal phenotype remains poorly understood. A unique monolayer cell-culture system was developed to induce, expose, and recapitulate the entire developmental series of events of subventricular zone (SVZ) neurogenesis. We show here, using immunophenotypic, ultrastructural, electrophysiological, and time-lapse analyses, that SVZ-derived glial fibrillary acidic protein(low)/A2B5+/nestin+ candidate founder cells undergo metamorphosis to eventually generate large numbers of fully differentiated interneuron phenotypes. A model of postnatal neurogenesis is considered in light of known embryonic events and reveals a limited developmental potential of SVZ stem/progenitor cells, whereby ancestral cells in both embryonic and postnatal/adult settings give rise to glia and GABAergic interneurons.
journal_name
Proc Natl Acad Sci U S Aauthors
Scheffler B,Walton NM,Lin DD,Goetz AK,Enikolopov G,Roper SN,Steindler DAdoi
10.1073/pnas.0503965102keywords:
subject
Has Abstractpub_date
2005-06-28 00:00:00pages
9353-8issue
26eissn
0027-8424issn
1091-6490pii
0503965102journal_volume
102pub_type
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