Characterization of a cDNA clone encoding the calmodulin-binding domain of mouse brain calcineurin.

abstract：:The receptor for acetylcholine was partially purified by affinity chromatography of an extract in Triton X-100 of membrane fragments from electric tissue. The receptor was assayed, after its reduction with dithiothreitol, by reaction with the affinity-alkylating agent, [methyl-(3)H]4-(N-maleimido)-benzyltrimethylammon...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin A,Cowburn D

更新日期：1973-12-01 00:00:00

abstract：:Recently it has become apparent that N = 2 supersymmetric quantum field theory has something to do with cluster algebras. I review one aspect of the connection: supersymmetric quantum field theories have associated hyperkähler moduli spaces, and these moduli spaces carry a structure that looks like an extension of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neitzke A

更新日期：2014-07-08 00:00:00

abstract：:The effects of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, on Na+ influx were investigated in cultured human foreskin fibroblasts (HSWP cells). We report here that in serum-deprived HSWP cells the addition of PMA alone has no significant effect on Na+ influx. However, the addition of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vicentini LM,Villereal ML

更新日期：1985-12-01 00:00:00

abstract：:Ventilator-induced diaphragmatic dysfunction (VIDD) refers to the diaphragm muscle weakness that occurs following prolonged controlled mechanical ventilation (MV). The presence of VIDD impedes recovery from respiratory failure. However, the pathophysiological mechanisms accounting for VIDD are still not fully understo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matecki S,Dridi H,Jung B,Saint N,Reiken SR,Scheuermann V,Mrozek S,Santulli G,Umanskaya A,Petrof BJ,Jaber S,Marks AR,Lacampagne A

更新日期：2016-08-09 00:00:00

abstract：:Whole-cell DNA preparations isolated from SC-1 cells chronically infected with N- or B-tropic murine leukemia viruses (MuLV) were tested for infectious activity in an Fv-1n (NIH-3T3) and two Fv-Ib (C57BL/6 and SV-A31) cell cultures. Efficiency of transfection for all DNAs was better in the NIH-3T3 cells than in C57BL/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu IC,Yang WK,Tennant RW,Brown A

更新日期：1978-03-01 00:00:00

abstract：:By commandeering cellular translation initiation factors, or destroying those dispensable for viral mRNA translation, viruses often suppress host protein synthesis. In contrast, cellular protein synthesis proceeds in human cytomegalovirus (HCMV)-infected cells, forcing viral and cellular mRNAs to compete for limiting ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McKinney C,Perez C,Mohr I

更新日期：2012-04-10 00:00:00

abstract：:The Snf1 protein kinase family has been conserved in eukaryotes. In the yeast Saccharomyces cerevisiae, Snf1 is essential for transcription of glucose-repressed genes in response to glucose starvation. The direct interaction between Snf1 and its activating subunit, Snf4, within the kinase complex is regulated by the g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ludin K,Jiang R,Carlson M

更新日期：1998-05-26 00:00:00

abstract：:A simple model for necking and detachment of subducting slabs is developed to include the coupling between grain-sensitive rheology and grain-size evolution with damage. Necking is triggered by thickened buoyant crust entrained into a subduction zone, in which case grain damage accelerates necking and allows for relat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bercovici D,Schubert G,Ricard Y

更新日期：2015-02-03 00:00:00

abstract：:Heat shock factors (HSFs) are the master regulators of transcription under protein-damaging conditions, acting in an environment where the overall transcription is silenced. We determined the genomewide transcriptional program that is rapidly provoked by HSF1 and HSF2 under acute stress in human cells. Our results rev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vihervaara A,Sergelius C,Vasara J,Blom MA,Elsing AN,Roos-Mattjus P,Sistonen L

更新日期：2013-09-03 00:00:00

abstract：:We have long known that language is lateralized to the left hemisphere (LH) in most neurologically healthy adults. In contrast, findings on lateralization of function during development are more complex. As in adults, anatomical, electrophysiological, and neuroimaging studies in infants and children indicate LH latera...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olulade OA,Seydell-Greenwald A,Chambers CE,Turkeltaub PE,Dromerick AW,Berl MM,Gaillard WD,Newport EL

更新日期：2020-09-22 00:00:00

abstract：:Plants sense phosphate (Pi) deficiency and initiate signaling that controls adaptive responses necessary for Pi acquisition. Herein, evidence establishes that AtSIZ1 is a plant small ubiquitin-like modifier (SUMO) E3 ligase and is a focal controller of Pi starvation-dependent responses. T-DNA insertional mutated allel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miura K,Rus A,Sharkhuu A,Yokoi S,Karthikeyan AS,Raghothama KG,Baek D,Koo YD,Jin JB,Bressan RA,Yun DJ,Hasegawa PM

更新日期：2005-05-24 00:00:00

abstract：:L-Asparaginase (EC 3.5.1.1) inhibited respiration in sensitive, but not resistant, lines of murine lymphoma 6C3HED. Glucose, in these tumor lines, was principally converted to lactate, and very little was oxidized in the citric acid cycle or hexose monophosphate shunt. The cells derived 70-80% of their respiratory CO(...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lavietes BB,Regan DH,Demopoulos HB

更新日期：1974-10-01 00:00:00

abstract：:C-1027 is an enediyne antitumor antibiotic composed of four distinct moieties: an enediyne core, a deoxy aminosugar, a beta-amino acid, and a benzoxazolinate moiety. We now show that the benzoxazolinate moiety is derived from chorismate by the sequential action of two enzymes-SgcD, a 2-amino-2-deoxyisochorismate (ADIC...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Lanen SG,Lin S,Shen B

更新日期：2008-01-15 00:00:00

abstract：:Enhanced ethylene production is an early response of plants to pathogen attack and has been associated with both resistance and susceptibility to disease. Tobacco plants were transformed with the mutant etr1-1 gene from Arabidopsis, conferring dominant ethylene insensitivity. Besides lacking known ethylene responses, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knoester M,van Loon LC,van den Heuvel J,Hennig J,Bol JF,Linthorst HJ

更新日期：1998-02-17 00:00:00

abstract：:Columnar stromatolites were abundant and widespread in the Proterozoic but are exceedingly rare in modern seas. Consequently, the stromatolites at Hamelin Pool in Shark Bay, Western Australia, have been widely interpreted as unique modern analogs of ancient stromatolites constructed by complex communities of cyanobact...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Awramik SM,Riding R

更新日期：1988-03-01 00:00:00

abstract：:Cells contain numerous enzymes that use molecular oxygen for their reactions. Often, their active sites are buried deeply inside the protein, which raises the question whether there are specific access channels guiding oxygen to the site of catalysis. Choosing 12/15-lipoxygenase as a typical example for such oxygen-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saam J,Ivanov I,Walther M,Holzhütter HG,Kuhn H

更新日期：2007-08-14 00:00:00

abstract：:The serological properties of the gag gene products p15 and p12 of N- and B-tropic viruses of C57BL mice have been examined. Although these viruses were serologically identical by competition assays for proteins gp71 and p30, they were readily distinguishable in competition assays for proteins p15 and p12. Two isolate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Benade LE,Ihle JN,Declève A

更新日期：1978-09-01 00:00:00

abstract：:The phosphorylation of H2Ax on its S139 site, γH2Ax, is important during DNA double-strand repair and is considered necessary for assembly of repair complexes, but its functional role after other kinds of DNA damage is less clear. We have measured the survival of isogenic mouse cell lines with the H2Ax gene knocked ou...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Revet I,Feeney L,Bruguera S,Wilson W,Dong TK,Oh DH,Dankort D,Cleaver JE

更新日期：2011-05-24 00:00:00

abstract：:Located within the brain's ventricles, the choroid plexus produces cerebrospinal fluid and forms an important barrier between the central nervous system and the blood. For unknown reasons, the choroid plexus produces high levels of the protein klotho. Here, we show that these levels naturally decline with aging. Deple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu L,Stein LR,Kim D,Ho K,Yu GQ,Zhan L,Larsson TE,Mucke L

更新日期：2018-11-27 00:00:00

abstract：:DNA polymerases replicate DNA by adding nucleotides to a growing primer strand while avoiding frameshift and point mutations. Here we present a series of up to six successive replication events that were obtained by extension of a primed template directly in a crystal of the thermostable Bacillus DNA polymerase I. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johnson SJ,Taylor JS,Beese LS

更新日期：2003-04-01 00:00:00

abstract：:The interaction of the NH2-terminal DNA-binding domain of lac repressor with synthetic oligo[d(AT)] was studied by a photo-CIDNP technique (CIDNP is chemically induced dynamic nuclear polarization). Three of the four tyrosines of the NH2-terminal region were found to be accessible to the photosensitive dye. The corres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Buck F,Rüterjans H,Kaptein R,Beyreuther K

更新日期：1980-09-01 00:00:00

abstract：:Cathepsin B (CTSB) is overexpressed in tumors of the lung, prostate, colon, breast, and stomach. However, evidence of primary genomic alterations in the CTSB gene during tumor initiation or progression has been lacking. We have found a novel amplicon at 8p22-23 that results in CTSB overexpression in esophageal adenoca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes SJ,Glover TW,Zhu XX,Kuick R,Thoraval D,Orringer MB,Beer DG,Hanash S

更新日期：1998-10-13 00:00:00

abstract：:Osteoclasts, bone-resorptive multinucleated cells derived from hematopoietic stem cells, are associated with many bone-related diseases, such as osteoporosis. Osteoclast-targeting small-molecule inhibitors are valuable tools for studying osteoclast biology and for developing antiresorptive agents. Here, we have discov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawatani M,Okumura H,Honda K,Kanoh N,Muroi M,Dohmae N,Takami M,Kitagawa M,Futamura Y,Imoto M,Osada H

更新日期：2008-08-19 00:00:00

abstract：:Prothoracicotropic hormone (PTTH) is the central cerebral neurohormone in insect development. Its release has been believed for decades to be confined to one (or two) critical moments early in each developmental stage at which time it triggers prolonged activation of the prothoracic glands to synthesize and release th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vafopoulou X,Steel CG

更新日期：1996-04-16 00:00:00

abstract：:An ovarian cell line was developed that requires hormonal conditioning of the host for growth in vivo and that requires special factors for growth in vitro. It is necessary to prepare special culture media to demonstrate the effects of growth factors in vitro. To this end, methods were developed for removing from seru...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishikawa K,Armelin HA,Sato G

更新日期：1975-02-01 00:00:00

abstract：:The T-cell antigen receptor alpha-chain genes of an alloreactive, H-2Db-specific cytotoxic T-cell clone (3F9) are described. This study and our work on the 3F9 beta-chain genes reveal that the variable region gene segments for the alpha and beta chains expressed in 3F9 are identical to the ones used by a chicken eryth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rupp F,Brecher J,Giedlin MA,Mosmann T,Zinkernagel RM,Hengartner H,Joho RH

更新日期：1987-01-01 00:00:00

abstract：:RelB is an NF-κB family transcription factor activated in the noncanonical pathway downstream of NF-κB-inducing kinase (NIK) and TNF receptor family members including lymphotoxin-β receptor (LTβR) and CD40. Early analysis suggested that RelB is required for classical dendritic cell (cDC) development based on a severe ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Briseño CG,Gargaro M,Durai V,Davidson JT,Theisen DJ,Anderson DA 3rd,Novack DV,Murphy TL,Murphy KM

更新日期：2017-04-11 00:00:00

abstract：:Transforming growth factor beta (TGF-beta)-mediated G(1) arrest previously has been shown to specifically target inactivation of cyclin D:cyclin-dependent kinase (Cdk) 4/6 complexes. We report here that TGF-beta-treated human HepG2 hepatocellular carcinoma cells arrest in G(1), but retain continued cyclin D:Cdk4/6 act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagahara H,Ezhevsky SA,Vocero-Akbani AM,Kaldis P,Solomon MJ,Dowdy SF

更新日期：1999-12-21 00:00:00

abstract：:The stability and integrity of mRNAs encoding neurotransmitter receptors and voltage-activated channels in the postmortem rat brain was investigated by isolating poly(A)+ mRNA, injecting it into Xenopus oocytes, and then examining the expression of functional neurotransmitter receptors and voltage-activated channels i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ragsdale DS,Miledi R

更新日期：1991-03-01 00:00:00

abstract：:[3H]Puromycin and N-(ethyl-2-diazomalonyl)[3H]puromycin are incorporated into E. coli ribosomes on irradiation at 253.7 nm. Both compounds incorporate into both protein and nucleic acid. Two-dimensional gel electrophoresis of ribosomal protein shows that L23 is the major protein labeled by puromycin. Although incorpor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cooperman BS,Jaynes EN,Brunswick DJ,Luddy MA

更新日期：1975-08-01 00:00:00