Abstract:
:We have developed a high-throughput approach to the labor-intensive problems of DNA cyclization, which we use to characterize DNA curvature and mechanical properties. The method includes a combinatorial approach to make the DNA constructs needed and automated real-time measurement of the kinetics using fluorescence. We validated the approach and investigated the flexibility of two kinds of nicked DNA and AT dinucleotide repeats. We found that, although the nicks hardly alter the bending flexibility, they significantly increase the torsional flexibility, and that the AT repeat has 28% (+/-12%) lower bending rigidity than a generic DNA sequence.
journal_name
Proc Natl Acad Sci U S Aauthors
Zhang Y,Crothers DMdoi
10.1073/pnas.0530189100keywords:
subject
Has Abstractpub_date
2003-03-18 00:00:00pages
3161-6issue
6eissn
0027-8424issn
1091-6490pii
0530189100journal_volume
100pub_type
杂志文章abstract::Catalysis at organophilic silica-rich surfaces of zeolites and feldspars might generate replicating biopolymers from simple chemicals supplied by meteorites, volcanic gases, and other geological sources. Crystal-chemical modeling yielded packings for amino acids neatly encapsulated in 10-ring channels of the molecular...
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