Switch recombination in normal IgA1+ B lymphocytes.

abstract：:Treatment strategies for severe cases of pandemic influenza have focused on antiviral therapies. In contrast, passive immunotherapy with convalescent blood products has received limited attention. We consider the hypothesis that a passive-immunotherapy program that collects plasma from a small percentage of recovered ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu JT,Lee CK,Cowling BJ,Yuen KY

更新日期：2010-02-16 00:00:00

abstract：:RPE65 is a protein of unknown function expressed specifically by the retinal pigment epithelium. We examined all 14 exons of this gene in 147 unrelated patients with autosomal recessive retinitis pigmentosa (RP), in 15 patients with isolate RP, and in 45 patients with Leber congenital amaurosis (LCA). Sequence anomali...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morimura H,Fishman GA,Grover SA,Fulton AB,Berson EL,Dryja TP

更新日期：1998-03-17 00:00:00

abstract：:Bacterial conjugation systems are members of the large type IV secretion system (T4SS) superfamily. Conjugative transfer of F plasmids residing in the Enterobacteriaceae was first reported in the 1940s, yet the architecture of F plasmid-encoded transfer channel and its physical relationship with the F pilus remain unk...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hu B,Khara P,Christie PJ

更新日期：2019-07-09 00:00:00

abstract：:We describe a single autoregulatory cassette that allows reversible induction of transgene expression in response to tetracycline (tet). This cassette contains all of the necessary components previously described by others on two separate plasmids that are introduced sequentially over a period of months [Gossen, M. & ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hofmann A,Nolan GP,Blau HM

更新日期：1996-05-28 00:00:00

abstract：:Spontaneous reciprocal mitotic recombination in the yeast Saccharomyces cerevisiae, associated with heteroallelic recombination, occurs almost exclusively at the two-strand stage and involves recombination of unduplicated chromosomes (i.e., during G1) or the unduplicated regions of chromosomes during the S phase of mi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Esposito MS

更新日期：1978-09-01 00:00:00

abstract：:The DNA sequence of a tryptophan synthase gene and the flanking 5' and 3' regions has been determined for Arabidopsis thaliana. The sequence encodes only the beta subunit domain, indicating that alpha and beta subunits are specified by separate genes. The gene contains four introns and encodes 470 amino acid residues....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berlyn MB,Last RL,Fink GR

更新日期：1989-06-01 00:00:00

abstract：:Okadaic acid is a non-phorbol 12-myristate 13-acetate (PMA)-type tumor promoter on mouse skin and known to be a potent inhibitor of serine/threonine protein phosphatases. Contrary to expectation from its tumor-promoting activity, okadaic acid was shown to have a potential to revert the phenotypes of cells transformed ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sakai R,Ikeda I,Kitani H,Fujiki H,Takaku F,Rapp U,Sugimura T,Nagao M

更新日期：1989-12-01 00:00:00

abstract：:The trace metals nickel and platinum, which are not substrates for ferrochelatase and thus do not form heme in biological systems, were found to act similaryl to cobalt, and heme itself, in regulating heme metabolism in liver and kidney. These metals induced heme oxygenase activity in both organs with the peak of indu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Maines MD,Kappas A

更新日期：1977-05-01 00:00:00

abstract：:Interspersed repeated sequences (Alu and Kpn) were used as probes to detect a set of Not I restriction fragments of human chromosome 21 from the hybrid cell line WAV17. Forty different Not I fragments, ranging in size from less than 0.05 megabase (Mb) to 7.0 Mb, were identified. The total length of these fragments was...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sainz J,Pevny L,Wu Y,Cantor CR,Smith CL

更新日期：1992-02-01 00:00:00

abstract：:ClpB is a ring-forming, ATP-dependent protein disaggregase that cooperates with the cognate Hsp70 system to recover functional protein from aggregates. How ClpB harnesses the energy of ATP binding and hydrolysis to facilitate the mechanical unfolding of previously aggregated, stress-damaged proteins remains unclear. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biter AB,Lee S,Sung N,Tsai FT

更新日期：2012-07-31 00:00:00

abstract：:We established stable COS-7 cell lines overexpressing recombinant PTPMEG and an inactive mutant form in which the active site cysteine is mutated to serine (PTPMEGCS). We found that both endogenous and recombinant enzyme were primarily located in the membrane and cytoskeletal fractions of COS-7 cells. Endogenous PTPME...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gu M,Meng K,Majerus PW

更新日期：1996-11-12 00:00:00

abstract：:The sequence of a nucleotide region of f1 bacteriophage was determined on a bonded ultrathin acrylamide gel with a discontinuous buffer system by using the dideoxy-DNA sequencing method. This sequence and one other were analyzed for maximal base pairing with tRNAs. The results allow a prediction of the direction and p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pieczenik G

更新日期：1980-06-01 00:00:00

abstract：:Unlike other predators that use vision as their primary sensory system, bats compute the three-dimensional (3D) position of flying insects from discrete echo snapshots, which raises questions about the strategies they employ to track and intercept erratically moving prey from interrupted sensory information. Here, we ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Salles A,Diebold CA,Moss CF

更新日期：2020-11-17 00:00:00

abstract：:Droplet manipulation is playing an important role in various fields, including scientific research, industrial production, and daily life. Here, inspired by the microstructures and functions of Namib desert beetles, Nepenthes pitcher plants, and emergent aquatic plants, we present a multibioinspired slippery surface f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang X,Sun L,Wang Y,Bian F,Wang Y,Zhao Y

更新日期：2019-10-15 00:00:00

abstract：:We report on the production of tumor necrosis factor (TNF)-alpha and TNF-beta by mitogen-activated peripheral blood lymphocytes or enriched monocyte subpopulations from human leukocyte antigen (HLA)-typed healthy subjects. The results indicate that HLA-DR2- and DQw1-positive donors frequently exhibit low production of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jacob CO,Fronek Z,Lewis GD,Koo M,Hansen JA,McDevitt HO

更新日期：1990-02-01 00:00:00

abstract：:Ferric uptake regulation protein (Fur) is a bacterial global regulator that uses iron as a cofactor to bind to specific DNA sequences. The function of Fur is not limited to iron homeostasis. A wide variety of genes involved in various mechanisms such as oxidative and acid stresses are under Fur control. Flavohemoglobi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： D'Autreaux B,Touati D,Bersch B,Latour JM,Michaud-Soret I

更新日期：2002-12-24 00:00:00

abstract：:Selective lodgement or homing of transplanted hemopoietic stem cells in the recipient's bone marrow (BM) is a critical step in the establishment of long-term hemopoiesis after BM transplantation. However, despite its biologic and clinical significance, little is understood about the process of homing. In the present s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Papayannopoulou T,Craddock C,Nakamoto B,Priestley GV,Wolf NS

更新日期：1995-10-10 00:00:00

abstract：:Helicobacter pylori VacA, a pore-forming toxin secreted by an autotransporter pathway, causes multiple alterations in human cells, contributes to the pathogenesis of peptic ulcer disease and gastric cancer, and is a candidate antigen for inclusion in an H. pylori vaccine. Here, we present a 2.4-A crystal structure of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gangwer KA,Mushrush DJ,Stauff DL,Spiller B,McClain MS,Cover TL,Lacy DB

更新日期：2007-10-09 00:00:00

abstract：:Aneuploidy is ubiquitous in cancer, and its phenotypes are inevitably dominant and abnormal. In view of these facts we recently proposed that aneuploidy is sufficient for carcinogenesis generating cancer-specific aneusomies via a chain reaction of autocatalytic aneuploidizations. According to this hypothesis a carcino...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fabarius A,Willer A,Yerganian G,Hehlmann R,Duesberg P

更新日期：2002-05-14 00:00:00

abstract：:Previously, we showed using a computational agent-based model that a group of animals moving together can make a collective decision on direction of motion, even if there is a conflict between the directional preferences of two small subgroups of "informed" individuals and the remaining "uninformed" individuals have n...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leonard NE,Shen T,Nabet B,Scardovi L,Couzin ID,Levin SA

更新日期：2012-01-03 00:00:00

abstract：:Rod signals in the mammalian retina are thought to reach ganglion cells over the circuit rod-->rod depolarizing bipolar cell-->AII amacrine cell-->cone bipolar cells-->ganglion cells. A possible alternative pathway involves gap junctions linking the rods and cones, the circuit being rod-->cone-->cone bipolar cells-->g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： DeVries SH,Baylor DA

更新日期：1995-11-07 00:00:00

abstract：:A number of genes associated with sexual traits and reproduction evolve at the sequence level faster than the majority of genes coding for non-sex-related traits. Whole genome analyses allow this observation to be extended beyond the limited set of genes that have been studied thus far. We use cDNA microarrays to demo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meiklejohn CD,Parsch J,Ranz JM,Hartl DL

更新日期：2003-08-19 00:00:00

abstract：:O(6)-methylguanine (O(6)meG) and related modifications of guanine in double-stranded DNA are functionally severe lesions that can be produced by many alkylating agents, including N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a potent carcinogen. O(6)meG is repaired through its demethylation by the O(6)-alkylguanine-DNA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hwang CS,Shemorry A,Varshavsky A

更新日期：2009-02-17 00:00:00

abstract：:OLE (ornate, large, extremophilic) RNAs comprise a class of structured noncoding RNAs (ncRNAs) found in many extremophilic bacteria species. OLE RNAs constitute one of the longest and most widespread bacterial ncRNA classes whose major biochemical function remains unknown. In the Gram-positive alkaliphile Bacillus hal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harris KA,Zhou Z,Peters ML,Wilkins SG,Breaker RR

更新日期：2018-07-03 00:00:00

abstract：:Physical and gene mapping studies reveal that chloroplast DNA from geranium (Pelargonium hortorum) has sustained a number of extensive duplications and inversions, resulting in a genome arrangement radically unlike that of other plants. At 217 kilobases in size, the circular chromosome is about 50% larger than the typ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palmer JD,Nugent JM,Herbon LA

更新日期：1987-02-01 00:00:00

abstract：:The composition, insertion, and turnover of externally disposed proteins on the macrophage plasma membrane were analyzed. Cells labeled with [35S]methionine were incubated with the nonpermeant reagent trinitrobenzene sulfonic acid to introduce the trinitrophenyl moiety on free amino groups of externally oriented membr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kaplan G,Unkeless JC,Cohn ZA

更新日期：1979-08-01 00:00:00

abstract：:DNA extracted from Dane particles has been characterized by gel electrophoresis and restriction enzyme cleavage with endonuclease R-HaeIII (from Hemophilus aegyptius). Dane particle DNA is proposed to be a double-stranded circular DNA approximately 3600 nucleotides in length containing a single-stranded gap of 600-210...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Summers J,O'Connell A,Millman I

更新日期：1975-11-01 00:00:00

abstract：:Transcription antitermination by lambda N gene product is affected in a mutant Escherichia coli with altered ribosomal protein S10, caused by the nusE71 mutation. To study the role of translation in antitermination, we have fused the phage nutR locus, the site of action of N, with the lac regulatory region. We have mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warren F,Das A

更新日期：1984-06-01 00:00:00

abstract：:Genome-wide experiments often measure quantitative differences between treated and untreated cells to identify affected strains. For these studies, statistical models are typically used to determine significance cutoffs. We developed a method termed "CLIK" (Cutoff Linked to Interaction Knowledge) that overlays biologi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dittmar JC,Pierce S,Rothstein R,Reid RJ

更新日期：2013-04-30 00:00:00

abstract：:Helix-hairpin-helix DNA glycosylases are typically small proteins that initiate repair of DNA by excising damaged or mispaired bases. An invariant aspartic acid in the active site is involved in catalyzing the excision reaction. Replacement of this critical residue with an asparagine severely reduces catalytic activit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Choi Y,Harada JJ,Goldberg RB,Fischer RL

更新日期：2004-05-11 00:00:00