Abstract:
:The DNA sequence of a tryptophan synthase gene and the flanking 5' and 3' regions has been determined for Arabidopsis thaliana. The sequence encodes only the beta subunit domain, indicating that alpha and beta subunits are specified by separate genes. The gene contains four introns and encodes 470 amino acid residues. The plant amino acid sequence is highly conserved with respect to corresponding microbial sequences. The NH2-terminal amino acid sequence is characteristic of chloroplast transit peptides. Identity of the sequences of the genomic exons and a cDNA clone and the presence of cellular RNA corresponding in size and 5' sequence to the gene indicate that the gene is expressed. Analysis of Arabidopsis genomic DNA suggests the presence of a second gene for the beta subunit.
journal_name
Proc Natl Acad Sci U S Aauthors
Berlyn MB,Last RL,Fink GRdoi
10.1073/pnas.86.12.4604subject
Has Abstractpub_date
1989-06-01 00:00:00pages
4604-8issue
12eissn
0027-8424issn
1091-6490journal_volume
86pub_type
杂志文章abstract::With the goal of developing techniques for DNA insertional mutagenesis in zebrafish, we established procedures for rapidly obtaining and injecting large numbers of fertilized eggs. Using either of two plasmid constructs, we injected uncut DNA into fertilized eggs at the one- or two-cell stage. Fish hatched from inject...
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journal_title:Proceedings of the National Academy of Sciences of the United States of America
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更新日期:2020-02-25 00:00:00