Functionalized xenon as a biosensor.

Abstract:

:The detection of biological molecules and their interactions is a significant component of modern biomedical research. In current biosensor technologies, simultaneous detection is limited to a small number of analytes by the spectral overlap of their signals. We have developed an NMR-based xenon biosensor that capitalizes on the enhanced signal-to-noise, spectral simplicity, and chemical-shift sensitivity of laser-polarized xenon to detect specific biomolecules at the level of tens of nanomoles. We present results using xenon "functionalized" by a biotin-modified supramolecular cage to detect biotin-avidin binding. This biosensor methodology can be extended to a multiplexing assay for multiple analytes.

authors

Spence MM,Rubin SM,Dimitrov IE,Ruiz EJ,Wemmer DE,Pines A,Yao SQ,Tian F,Schultz PG

doi

10.1073/pnas.191368398

keywords:

subject

Has Abstract

pub_date

2001-09-11 00:00:00

pages

10654-7

issue

19

eissn

0027-8424

issn

1091-6490

pii

191368398

journal_volume

98

pub_type

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