Ubiquitin-mediated sequestration of normal cellular proteins into polyglutamine aggregates.

abstract：:The ATR (ATM- and rad3-related)-mediated checkpoint pathway has a crucial role in regulating the cellular responses to DNA damage and DNA-replication stress. ATRIP (ATR-interacting protein), the regulatory partner of ATR, binds directly to replication protein A (RPA)-coated ssDNA and enables the ATR-ATRIP complex to r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Namiki Y,Zou L

更新日期：2006-01-17 00:00:00

abstract：:In yeast, the mRNA encoded by the MAT alpha 1 gene is unstable (t1/2 = 5 min) and the mRNAs encoded by the ACT1 gene (t1/2 = 30 min) and the PGK1 gene (t1/2 = 45 min) are stable. To understand the RNA structural features that dictate mRNA decay rates in yeast, we have constructed PGK1/MAT alpha 1 and ACT1/MAT alpha 1 ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parker R,Jacobson A

更新日期：1990-04-01 00:00:00

abstract：:It has been shown that [35S]methionine is incorporated into the [Met]enkephalin sequences of a 22,000-dalton enkephalin-containing protein in the adrenal medulla. Pulse-chase experiments indicate that label is incorporated into the large polypeptide before it appears in free [Met]enkephalin and a smaller [Met]enkephal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rossier J,Trifaró JM,Lewis RV,Lee RW,Stern A,Kimura S,Stein S,Udenfriend S

更新日期：1980-11-01 00:00:00

abstract：:The amount of information that can be stored in working memory is limited but may be improved with practice. The basis of improved efficiency at the level of neural activity is unknown. To investigate this question, we trained monkeys to perform a working memory task that required memory for multiple stimuli. Performa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tang H,Qi XL,Riley MR,Constantinidis C

更新日期：2019-04-02 00:00:00

abstract：:Previously, recombinant human respiratory syncytial virus (RSV) monoclonal antibody Fabs were generated by antigen selection from random combinatorial libraries displayed at the tip of filamentous phage. Two such Fabs, which exhibited high binding affinity for RSV F glycoprotein (a major protective antigen), were eval...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Crowe JE Jr,Murphy BR,Chanock RM,Williamson RA,Barbas CF 3rd,Burton DR

更新日期：1994-02-15 00:00:00

abstract：:Cyclooxygenase (COX)-2, a rate-limiting enzyme of prostaglandin (PG) production, is overexpressed in colorectal adenomas and adenocarcinomas, and its inhibition by nonsteroidal antiinflammatory drugs protects against colorectal cancer. Mechanisms of cancer promotion by COX-2 are not fully understood, but signaling thr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishihara H,Kizaka-Kondoh S,Insel PA,Eckmann L

更新日期：2003-07-22 00:00:00

abstract：:Translation of injected mRNA in oocytes of Xenopus laevis has been used as a highly sensitive and quantitative assay for interferon mRNA. Injection into oocytes of polyadenylylated RNA extracted from poly(I).poly(C)-induced human diploid fibroblasts (FS-4) leads to the synthesis of biologically active human fibroblast...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sehgal PB,Dobberstein B,Tamm I

更新日期：1977-08-01 00:00:00

abstract：:Whole-genome duplication (WGD) is believed to be a significant source of major evolutionary innovation. Redundant genes resulting from WGD are thought to be lost or acquire new functions. However, the rates of gene loss and thus temporal process of genome reshaping after WGD remain unclear. The WGD shared by all teleo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Inoue J,Sato Y,Sinclair R,Tsukamoto K,Nishida M

更新日期：2015-12-01 00:00:00

abstract：:Total heterogeneous nuclear RNA from HeLa cells infected with adenovirus for 18-20 hr stimulates amino acid incorporation into protein in a cell-free system from Ehrlich ascites tumors. This stimulation of protein synthesis by the nuclear RNA requires intact nuclei. The role of nuclei in this system is unknown, but ev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chatterjee NK,Weissbach H

更新日期：1974-08-01 00:00:00

abstract：:Lipoxin B (LXB) was prepared by incubation of (15S)-15-hydroperoxy-5,8,11-cis-13-trans-icosatetraenoic acid (15-HPETE) with human leukocytes. Comparison with a number of trihydroxyicosatetraenes prepared by total synthesis showed that biologically derived LXB is (5S,14R,15S)-5,14,15-trihydroxy-6,10,12-trans-8-cis-icos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Serhan CN,Hamberg M,Samuelsson B,Morris J,Wishka DG

更新日期：1986-04-01 00:00:00

abstract：:The proinflammatory and catabolic cytokine IL-1β has been implicated in the pathogenesis of osteoarthritis (OA) by mediating synovial inflammation and cartilage degeneration. Although synovial macrophages are suggested to be the source of IL-1β, the mechanism remains unclear. Ectopic deposition of hydroxyapatite (HA) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin C,Frayssinet P,Pelker R,Cwirka D,Hu B,Vignery A,Eisenbarth SC,Flavell RA

更新日期：2011-09-06 00:00:00

abstract：:The mechanisms ensuring balanced growth remain a critical question in developmental biology. In plants, this balance relies on spatiotemporal integration of hormonal signaling pathways, but the understanding of the precise contribution of each hormone is just beginning to take form. Brassinosteroid (BR) hormone is sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vragović K,Sela A,Friedlander-Shani L,Fridman Y,Hacham Y,Holland N,Bartom E,Mockler TC,Savaldi-Goldstein S

更新日期：2015-01-20 00:00:00

abstract：:Pathologic ocular angiogenesis is a leading cause of blindness, influenced by both dysregulated lipid metabolism and inflammation. Retinoic-acid-receptor-related orphan receptor alpha (RORα) is a lipid-sensing nuclear receptor with diverse biologic function including regulation of lipid metabolism and inflammation; ho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun Y,Liu CH,SanGiovanni JP,Evans LP,Tian KT,Zhang B,Stahl A,Pu WT,Kamenecka TM,Solt LA,Chen J

更新日期：2015-08-18 00:00:00

abstract：:Interleukin (IL)-15 and its specific receptor chain, IL-15Rα, support the development of various effector cells, including NK and CD8 T cells via a mechanism called trans-presentation. Whereas the dynamic of trans-presentation has been shown to involve the recycling of IL-15Rα by presenting cells, the way responding c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tamzalit F,Barbieux I,Plet A,Heim J,Nedellec S,Morisseau S,Jacques Y,Mortier E

更新日期：2014-06-10 00:00:00

abstract：:In spite of the critical role of the process of adaptation in evolution, there are few detailed studies of the genotypic and molecular basis of the process. Drosophila melanogaster flies selected for increased tolerance to ethanol exhibited higher levels of alcohol dehydrogenase (alcohol:NAD+ oxidoreductase; EC 1.1.1....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McDonald JF,Chambers GK,David J,Ayala FJ

更新日期：1977-10-01 00:00:00

abstract：:The stoichiometry of J chain in pentamer IgM has been determined by measuring the radiolabeled thiols in the constituent chains after complete reduction and alkylation of the polymer. One mole of J was found to be disulfide bonded to 1 mol of pentamer. The linkage of J chain in IgM has been determined by correlating t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chapuis RM,Koshland ME

更新日期：1974-03-01 00:00:00

abstract：:Cyclooxygenase-2 (COX-2) expression is induced by mitogenic and proinflammatory factors. Its overexpression plays a causal role in inflammation and tumorigenesis. COX-2 expression is tightly regulated, but the mechanisms are largely unclear. Here we show the control of COX-2 expression by an endogenous tryptophan meta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheng HH,Kuo CC,Yan JL,Chen HL,Lin WC,Wang KH,Tsai KK,Guvén H,Flaberg E,Szekely L,Klein G,Wu KK

更新日期：2012-08-14 00:00:00

abstract：:The embryonic neuroepithelium gives rise to the components of the central nervous system in the mature animal. To study the early development of the murine central nervous system we have sought to isolate growth factor receptors from the neuroepithelium of the neural tube of 10-day-old mouse embryos. Because many grow...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reid HH,Wilks AF,Bernard O

更新日期：1990-02-01 00:00:00

abstract：:A mechanistic understanding of the controls on carbon storage and losses is essential for our capacity to predict and mitigate human impacts on the global carbon cycle. Plant litter decomposition is an important first step for carbon and nutrient turnover, and litter inputs and losses are essential in determining soil...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Austin AT,Méndez MS,Ballaré CL

更新日期：2016-04-19 00:00:00

abstract：:A unique property of the mammalian embryo is that stem cells can be derived from its early tissue lineages. These lineages will give rise to the fetus as well as essential extraembryonic tissues. Understanding how chromatin regulation participates in establishment of these lineages in the embryo and their derived stem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rugg-Gunn PJ,Cox BJ,Ralston A,Rossant J

更新日期：2010-06-15 00:00:00

abstract：:Gastrointestinal infections often induce epithelial damage that must be repaired for optimal gut function. While intestinal stem cells are critical for this regeneration process [R. C. van der Wath, B. S. Gardiner, A. W. Burgess, D. W. Smith, PLoS One 8, e73204 (2013); S. Kozar et al., Cell Stem Cell 13, 626-633 (2013...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mileto SJ,Jardé T,Childress KO,Jensen JL,Rogers AP,Kerr G,Hutton ML,Sheedlo MJ,Bloch SC,Shupe JA,Horvay K,Flores T,Engel R,Wilkins S,McMurrick PJ,Lacy DB,Abud HE,Lyras D

更新日期：2020-04-07 00:00:00

abstract：:DNA replication intermediates in human melanoma cells have been investigated by using the drug aphidicolin, which inhibits DNA polymerase alpha. In untreated cells, Okazaki fragments and 10-kilobase (kb) DNA intermediates are formed. In aphidicolin-treated cells, the replication fork is stopped and there is no formati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lönn U,Lönn S

更新日期：1983-07-01 00:00:00

abstract：:Epidermal growth factor (EGF) stimulates membrane protein phosphorylation in a human cell line, A-431. The known hepatic mitogenic action of EGF and the reduction in EGF receptor number that occurs during liver regeneration led us to study whether EGF-dependent protein kinase activity was present in rat liver and whet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rubin RA,O'Keefe EJ,Earp HS

更新日期：1982-02-01 00:00:00

abstract：:A patterning method termed "RIPPLE" (reactive interface patterning promoted by lithographic electrochemistry) is applied to the fabrication of arrays of dielectric and metallic optical elements. This method uses cyclic voltammetry to impart patterns onto the working electrode of a standard three-electrode electrochemi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kempa TJ,Bediako DK,Kim SK,Park HG,Nocera DG

更新日期：2015-04-28 00:00:00

abstract：:Ischemia and reperfusion both contribute to tissue damage after myocardial infarction. Although many drugs have been shown to reduce infarct size when administered before ischemia, few have been shown to be effective when administered at reperfusion. Moreover, although it is generally accepted that a burst of reactive...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Granville DJ,Tashakkor B,Takeuchi C,Gustafsson AB,Huang C,Sayen MR,Wentworth P Jr,Yeager M,Gottlieb RA

更新日期：2004-02-03 00:00:00

abstract：:The origin of the multicellular animals has been investigated by rate invariant analysis of 18S rRNA sequences. These analyses indicate that (i) the Metazoa is a monophyletic taxon; (ii) the Deuterostomia is a monophyletic taxon; (iii) the Annelida-Mollusca lineage is the sister group of an arthropod subgroup; and (iv...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lake JA

更新日期：1990-01-01 00:00:00

abstract：:In vitro incubation of leukemic bone marrow with the lipophilic fluorescent dye merocyanine 540 and simultaneous exposure to light reduced the concentration of L1210 leukemia cells by about 5 orders of magnitude but spared enough normal pluripotent hematopoietic stem cells to allow hematopoietic reconstitution of leth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sieber F,Spivak JL,Sutcliffe AM

更新日期：1984-12-01 00:00:00

abstract：:Cartilage collagen fibrils, which are characterized by their thin, uniform diameters, are formed of a multicomponent system of three collagen types (II, IX, and XI) and interacting proteoglycans. We have used a genetic approach to test whether the proper assembly of this multiprotein structure was altered by overexpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garofalo S,Metsäranta M,Ellard J,Smith C,Horton W,Vuorio E,de Crombrugghe B

更新日期：1993-05-01 00:00:00

abstract：:A deficit in early clearance of Pseudomonas aeruginosa (P. aeruginosa) is crucial in nosocomial pneumonia and in chronic lung infections. Few studies have addressed the role of Toll-like receptors (TLRs), which are early pathogen associated molecular pattern receptors, in pathogen uptake and clearance by alveolar macr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Descamps D,Le Gars M,Balloy V,Barbier D,Maschalidi S,Tohme M,Chignard M,Ramphal R,Manoury B,Sallenave JM

更新日期：2012-01-31 00:00:00

abstract：:Most human tumors are clonal in origin, although the cells may be diverse in their properties. Since the tumors evolve through progressive stages over decades of time, it is possible that the conditions that induced the tumor transform many cells, but that selective overgrowth of the fastest growing lead to a clonal p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yao A,Rubin H

更新日期：1993-11-15 00:00:00