Efficient transduction of neural cells in vitro and in vivo by a baculovirus-derived vector.

abstract：:It is well established that assimilatory nitrate reductase (ANR) activity in soil is inhibited by ammonium (NH4+). To elucidate the mechanism of this inhibition, we studied the effect of L-methionine sulfoximine (MSX), an inhibitor of NH4+ assimilation by microorganisms, on assimilatory reduction of nitrate (NO3-) in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCarty GW,Bremner JM

更新日期：1992-01-15 00:00:00

abstract：:Knowledge of how insects are actually affected by sex pheromones deployed throughout a crop so as to disrupt mating has lacked a mechanistic framework sufficient for guiding optimization of this environmentally friendly pest-control tactic. Major hypotheses are competitive attraction, desensitization, and camouflage. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller JR,McGhee PS,Siegert PY,Adams CG,Huang J,Grieshop MJ,Gut LJ

更新日期：2010-01-05 00:00:00

abstract：:Cell surface receptor membrane localization is strongly dependent on protein-protein interactions often involving regulation by phosphorylation/dephosphorylation of the intracellular domains of membrane proteins. The present study was carried out to identify metabotropic glutamate receptor (mGluR) 3 regulatory binding...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flajolet M,Rakhilin S,Wang H,Starkova N,Nuangchamnong N,Nairn AC,Greengard P

更新日期：2003-12-23 00:00:00

abstract：:The oligodendrocyte (Od), a glial cell that produces myelin in the central nervous system, may be a target for autoreactive T cells in autoimmune demyelinating processes, although not expressing major histocompatibility complex (MHC) products. To analyze Od-T-cell interactions, we selected from normal SJL/J mouse sple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jewtoukoff V,Lebar R,Bach MA

更新日期：1989-04-01 00:00:00

abstract：:Identified over a dozen years ago in the brain and pancreatic islet, βIV-spectrin is critical for the local organization of protein complexes throughout the nervous system. βIV-Spectrin targets ion channels and adapter proteins to axon initial segments and nodes of Ranvier in neurons, and βIV-spectrin dysfunction unde...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kline CF,Wright PJ,Koval OM,Zmuda EJ,Johnson BL,Anderson ME,Hai T,Hund TJ,Mohler PJ

更新日期：2013-10-22 00:00:00

abstract：:A Mr 70,000 protein was isolated from cytotoxic human large granular lymphocytes and shown to have cytotoxic activity. The protein was demonstrated to be immunochemically related to the ninth component (C9) of complement and was therefore designated C9-related protein (C9RP). This finding suggests that C9RP and C9 sha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zalman LS,Brothers MA,Chiu FJ,Müller-Eberhard HJ

更新日期：1986-07-01 00:00:00

abstract：:The intracellular signaling mechanisms regulating the generation and long-term persistence of memory T cells in vivo remain unclear. In this study, we used mouse models with conditional deletion of the key T cell receptor (TCR)-coupled adaptor molecule SH2-domain-containing phosphoprotein of 76 kDa (SLP-76), to analyz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bushar ND,Corbo E,Schmidt M,Maltzman JS,Farber DL

更新日期：2010-01-12 00:00:00

abstract：:Unfolded RNase A is known to contain an equilibrium mixture of two forms, a slow-folding form (U(1)) and a fast-folding form (U(2)). If U(1) is produced after unfolding by the slow cis-trans isomerization of proline residues about X-Pro imide bonds, then the formation of U(1) should be catalyzed by strong acids. There...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmid FX,Baldwin RL

更新日期：1978-10-01 00:00:00

abstract：:Predicting climate change impact on ecosystem structure and services is one of the most important challenges in ecology. Until now, plant species response to climate change has been described at the level of fixed plant functional types, an approach limited by its inflexibility as there is much interspecific functiona...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soudzilovskaia NA,Elumeeva TG,Onipchenko VG,Shidakov II,Salpagarova FS,Khubiev AB,Tekeev DK,Cornelissen JH

更新日期：2013-11-05 00:00:00

abstract：:Redistribution of specialized molecules in migrating cells develops asymmetry between two opposite cell poles, the leading edge and the uropod. We show that acquisition of a motile phenotype in T lymphocytes results in the asymmetric redistribution of ganglioside GM3- and GM1-enriched raft domains to the leading edge ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gómez-Móuton C,Abad JL,Mira E,Lacalle RA,Gallardo E,Jiménez-Baranda S,Illa I,Bernad A,Mañes S,Martínez-A C

更新日期：2001-08-14 00:00:00

abstract：:Although current theories of color vision differ in many respects, they all assume the existence of a uniquely defined neutral point in chromaticity space. It generally is assumed that this point satisfies several criteria simultaneously. One of these criteria is that it is perceived as achromatic. A further criterion...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ekroll V,Faul F,Niederée R,Richter E

更新日期：2002-10-01 00:00:00

abstract：:Cell-based or pharmacological approaches for promoting tendon repair are currently not available because the molecular mechanisms of tendon development and healing are not well understood. Although analysis of knockout mice provides many critical insights, small animals such as mice have some limitations. In particula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suzuki H,Ito Y,Shinohara M,Yamashita S,Ichinose S,Kishida A,Oyaizu T,Kayama T,Nakamichi R,Koda N,Yagishita K,Lotz MK,Okawa A,Asahara H

更新日期：2016-07-12 00:00:00

abstract：:Betidamino acids (a contraction of "beta" position and "amide") are N'-monoacylated (optionally, N'-monoacylated and N-mono- or N,N'-dialkylated) aminoglycine derivatives in which each N'acyl/alkyl group may mimic naturally occurring amino acid side chains or introduce novel functionalities. Betidamino acids are most ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rivier JE,Jiang G,Koerber SC,Porter J,Simon L,Craig AG,Hoeger CA

更新日期：1996-03-05 00:00:00

abstract：:Rotavirus is considered a directly transmitted disease due to its high infectivity. Environmental pathways have, therefore, largely been ignored. Rotavirus, however, persists in water sources, and both its surface water concentrations and infection incidence vary with temperature. Here, we examine the potential for wa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kraay ANM,Brouwer AF,Lin N,Collender PA,Remais JV,Eisenberg JNS

更新日期：2018-03-20 00:00:00

abstract：:Helper T (Th) cells are classified as Th1 or Th2 cells by virtue of cytokine secretion and function as mediators of cellular or humoral immunity, respectively. Cytokines also regulate the differentiation of Th cells. For example, interleukin (IL)-12 promotes Th1 and suppresses Th2 cell development, suggesting that IL-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Milich DR,Wolf SF,Hughes JL,Jones JE

更新日期：1995-07-18 00:00:00

abstract：:Recent technical advances allow detection of several hundred volatile organic compounds (VOCs) in human exhaled air, many of which reflect unidentified endogenous pathways. Our group has previously estimated plasma glucose levels in healthy adults during a standard oral glucose tolerance test via exhaled VOC analysis....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Novak BJ,Blake DR,Meinardi S,Rowland FS,Pontello A,Cooper DM,Galassetti PR

更新日期：2007-10-02 00:00:00

abstract：:Major histocompatibility complex (MHC) class II molecules are heterodimeric glycoproteins with one alpha and one beta polypeptide chain of similar molecular size. In this report, we describe the binding of an acetylated N-terminal peptide of myelin basic protein, [Ala4]MBP-(1-14), to purified individual alpha and beta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nag B,Wada HG,Deshpande SV,Passmore D,Kendrick T,Sharma SD,Clark BR,McConnell HM

更新日期：1993-02-15 00:00:00

abstract：:The way general anesthetics cause their clinically useful effects on the nervous system is not known. Since the principal determinant of the potency of these agents is their solubility in oil, the role of chemical structure in affecting anesthetic targets has been obscured. In this work, we use an intense beam of ligh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Campbell DB,Nash HA

更新日期：1994-03-15 00:00:00

abstract：:We have investigated the role of cAMP in NO- and prostanoid-independent relaxations that are widely attributed to an endothelium-derived hyperpolarizing factor (EDHF). Under control conditions EDHF-type relaxations evoked by acetylcholine (ACh) in rabbit iliac arteries were transient, but in the presence of the cAMP p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Griffith TM,Chaytor AT,Taylor HJ,Giddings BD,Edwards DH

更新日期：2002-04-30 00:00:00

abstract：:When a local mechanical perturbation is applied to the surface of a thin film of a mechanically interlocked molecule (a rotaxane), the molecules self-organize into periodic arrays of discrete dots or lines. The dimensionality of the nanostructures depends on whether the mechanical stimulus acts along a 1D line or over...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biscarini F,Cavallini M,Kshirsagar R,Bottari G,Leigh DA,León S,Zerbetto F

更新日期：2006-11-21 00:00:00

abstract：:Prolonged incubation of NIH 3T3 cells under the growth constraint of confluence results in a persistent impairment of proliferation when the cells are subcultured at low density and a greatly increased probability of neoplastic transformation in assays for transformation. These properties, along with the large accumul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chow M,Rubin H

更新日期：1996-09-03 00:00:00

abstract：:The role of rising atmospheric CO2 in modulating estuarine carbonate system dynamics remains poorly characterized, likely due to myriad processes driving the complex chemistry in these habitats. We reconstructed the full carbonate system of an estuarine seagrass habitat for a summer period of 2.5 months utilizing a co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pacella SR,Brown CA,Waldbusser GG,Labiosa RG,Hales B

更新日期：2018-04-10 00:00:00

abstract：:Guanylyl cyclase C (GCC) has been detected only in intestinal mucosa and colon carcinoma cells of placental mammals. However, this receptor has been identified in several tissues in marsupials, and its expression has been suggested in tissues other than intestine in placental mammals. Selective expression of GCC by co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carrithers SL,Barber MT,Biswas S,Parkinson SJ,Park PK,Goldstein SD,Waldman SA

更新日期：1996-12-10 00:00:00

abstract：:To gain further insight into the mode of action of S-locus receptor kinase (SRK), a receptor-like kinase involved in the self-incompatibility response in Brassica, different recombinant SRK proteins have been expressed in a membranous environment using the insect cell/baculovirus system. Recombinant SRK proteins exhib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Giranton JL,Dumas C,Cock JM,Gaude T

更新日期：2000-03-28 00:00:00

abstract：:On illuminating chloroplasts with "short-wavelength" monochromatic light that supports oxygen evolution, spectral evidence was obtained for a new photoreactive chloroplast component, provisionally designated C550, which shows a reversible decrease of absorbance with a maximum at 550 mmu. The light-induced absorbance c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knaff DB,Arnon DI

更新日期：1969-07-01 00:00:00

abstract：:A whole genome cattle-hamster radiation hybrid cell panel was used to construct a map of 54 markers located on bovine chromosome 5 (BTA5). Of the 54 markers, 34 are microsatellites selected from the cattle linkage map and 20 are genes. Among the 20 mapped genes, 10 are new assignments that were made by using the compa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ozawa A,Band MR,Larson JH,Donovan J,Green CA,Womack JE,Lewin HA

更新日期：2000-04-11 00:00:00

abstract：:The elevation of Ca2+ levels in the cytoplasm inactivates inward-rectifying K+ channels that play a central role in regulating the apertures of stomatal pores in higher plants. However, the mechanism for the Ca(2+)-mediated inhibition of K(+)-channel function is unknown. Using patch-clamp techniques, we show that cycl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luan S,Li W,Rusnak F,Assmann SM,Schreiber SL

更新日期：1993-03-15 00:00:00

abstract：:The phytohormone cytokinin influences many aspects of plant growth and development, several of which also involve the cellular process of autophagy, including leaf senescence, nutrient remobilization, and developmental transitions. The Arabidopsis type-A response regulators (type-A ARR) are negative regulators of cyto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acheampong AK,Shanks C,Cheng CY,Schaller GE,Dagdas Y,Kieber JJ

更新日期：2020-10-27 00:00:00

abstract：:The levels of c-myc, c-fos, and JE mRNAs accumulate in a biphasic pattern following infection of quiescent BALB/c 3T3 mouse cells with polyomavirus. Maximal levels of c-myc and c-fos mRNAs were seen within 1 hr and were nearly undetectable at 6 hr after infection. At 12 hr after infection mRNA levels were again maxima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zullo J,Stiles CD,Garcea RL

更新日期：1987-03-01 00:00:00

abstract：:Excess activation of glutamate receptors and production of free radicals including nitric oxide may result in severe and irreversible damage to the mammalian central nervous system (CNS), but endogenous defense systems that protect neurons from these insults are poorly understood. Here, we purified and isolated a neur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kume T,Asai N,Nishikawa H,Mano N,Terauchi T,Taguchi R,Shirakawa H,Osakada F,Mori H,Asakawa N,Yonaga M,Nishizawa Y,Sugimoto H,Shimohama S,Katsuki H,Kaneko S,Akaike A

更新日期：2002-03-05 00:00:00