Use of Drosophila mutants to distinguish among volatile general anesthetics.

abstract：:Immunoglobulin- or multiple myeloma-associated amyloidosis has been distinguished by the tissue deposition of Congophilic, fibrillar protein consisting of light chains or light-chain fragments (AL amyloidosis). We now report the isolation and characterization of another form of immunoglobulin-associated amyloid obtain...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eulitz M,Weiss DT,Solomon A

更新日期：1990-09-01 00:00:00

abstract：:DNA replication intermediates in human melanoma cells have been investigated by using the drug aphidicolin, which inhibits DNA polymerase alpha. In untreated cells, Okazaki fragments and 10-kilobase (kb) DNA intermediates are formed. In aphidicolin-treated cells, the replication fork is stopped and there is no formati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lönn U,Lönn S

更新日期：1983-07-01 00:00:00

abstract：:Compared with understanding of biological shape and form, knowledge is sparse regarding what regulates growth and body size of a species. For example, the genetic and physiological causes of heterosis (hybrid vigor) have remained elusive for nearly a century. Here, we investigate gene-expression patterns underlying gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hedgecock D,Lin JZ,DeCola S,Haudenschild CD,Meyer E,Manahan DT,Bowen B

更新日期：2007-02-13 00:00:00

abstract：:Two RNA fractions have been isolated and purified from both oncogenic and nononcogenic strains of Agrobacterium tumefaciens. Both RNAs are capable of inducing the formation of transplantable tumors when introduced at wound sites in stems of Datura stramonium plants. One of these RNA fractions was found to be bound to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beljanski M,Aaron-da Cunha MI,Beljanski MS,Manigault P,Bourgarel P

更新日期：1974-05-01 00:00:00

abstract：:Loquacious-PD (Loqs-PD) is required for biogenesis of many endogenous siRNAs in Drosophila In vitro, Loqs-PD enhances the rate of dsRNA cleavage by Dicer-2 and also enables processing of substrates normally refractory to cleavage. Using purified components, and Loqs-PD truncations, we provide a mechanistic basis for L...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trettin KD,Sinha NK,Eckert DM,Apple SE,Bass BL

更新日期：2017-09-19 00:00:00

abstract：:We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted int...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suggs SV,Wallace RB,Hirose T,Kawashima EH,Itakura K

更新日期：1981-11-01 00:00:00

abstract：:Mice lacking the beta1-subunit (gene, Kcnmb1; protein, BK-beta1) of the large Ca-activated K channel (BK) are hypertensive. This phenotype is thought to result from diminished BK currents in vascular smooth muscle where BK-beta1 is an ancillary subunit. However, the beta1-subunit is also expressed in the renal connect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grimm PR,Irsik DL,Settles DC,Holtzclaw JD,Sansom SC

更新日期：2009-07-14 00:00:00

abstract：:We investigated the structural and functional alterations of active lamellae during initial cell-cell collision and establishment of cell-cell contacts in wounded cultures of nontransformed rat epitheliocytes (IAR-2 line) and their ras-transformed descendants (C4 line). Typically, the leading edges of nontransformed c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gloushankova NA,Alieva NA,Krendel MF,Bonder EM,Feder HH,Vasiliev JM,Gelfand IM

更新日期：1997-02-04 00:00:00

abstract：:A 32-base-pair (bp) DNA duplex with deletions in one strand, and thus extra bases in the opposing strand, was ligated head-to-tail to produce linear and circular multimers. The electrophoretic mobility of the linear multimers was analyzed in polyacrylamide gels and the size of the circular DNA was determined by electr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsieh CH,Griffith JD

更新日期：1989-07-01 00:00:00

abstract：:The highly homologous Rnf2 (Ring1b) and Ring1 (Ring1a) proteins were identified as in vivo interactors of the Polycomb Group (PcG) protein Bmi1. Functional ablation of Rnf2 results in gastrulation arrest, in contrast to relatively mild phenotypes in most other PcG gene null mutants belonging to the same functional gro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voncken JW,Roelen BA,Roefs M,de Vries S,Verhoeven E,Marino S,Deschamps J,van Lohuizen M

更新日期：2003-03-04 00:00:00

abstract：:R. A. Fisher's 1930 "reproductive value" is defined as the contribution made by a population's initial age elements to its asymptotically dominating exponential growth mode. For the Leslie discrete-time model, it is the characteristic row vector of the Leslie matrix, and for the integral-equation model of Lotka the si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samuelson PA

更新日期：1977-11-01 00:00:00

abstract：:A climate/vegetation model simulates episodic wetter and drier periods at the 21,000-y precession period in eastern North Africa, the Arabian Peninsula, and the Levant over the past 140,000 y. Large orbitally forced wet/dry extremes occur during interglacial time, ∼130 to 80 ka, and conditions between these two extrem...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kutzbach JE,Guan J,He F,Cohen AS,Orland IJ,Chen G

更新日期：2020-02-04 00:00:00

abstract：:Oncogenic mutations in the small GTPase KRAS are frequently found in human cancers, and, currently, there are no effective targeted therapies for these tumors. Using a combinatorial siRNA approach, we analyzed a panel of KRAS mutant colorectal and pancreatic cancer cell lines for their dependency on 28 gene nodes that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee CS,Lee LC,Yuan TL,Chakka S,Fellmann C,Lowe SW,Caplen NJ,McCormick F,Luo J

更新日期：2019-03-05 00:00:00

abstract：:Interferon γ-inducible protein 16 (IFI16) and cGMP-AMP synthase (cGAS) have both been proposed to detect herpesviral DNA directly in herpes simplex virus (HSV)-infected cells and initiate interferon regulatory factor-3 signaling, but it has been unclear how two DNA sensors could both be required for this response. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Orzalli MH,Broekema NM,Diner BA,Hancks DC,Elde NC,Cristea IM,Knipe DM

更新日期：2015-04-07 00:00:00

abstract：:Rat liver glutamine synthetase (s(20,w) 15.0 S; MW about 352,000) resembles ovine brain glutamine synthetase in that it (a) has 8 subunits, (b) acts on both L- and D-glutamate and certain glutamate analogs (e.g., beta-glutamate, cis-cycloglutamate, and alpha-methyl-L-glutamate), and (c) is irreversibly inhibited by L-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tate SS,Meister A

更新日期：1971-04-01 00:00:00

abstract：:Some patients with a lesion to the primary visual cortex (V1) show "blindsight": the remarkable ability to guess correctly about attributes of stimuli presented to the blind hemifield. Here, we show that blindsight can be induced in normal observers by using transcranial magnetic stimulation of the occipital cortex bu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jolij J,Lamme VA

更新日期：2005-07-26 00:00:00

abstract：:The necroptosis cell death pathway has been implicated in host defense and in the pathology of inflammatory diseases. While phosphorylation of the necroptotic effector pseudokinase Mixed Lineage Kinase Domain-Like (MLKL) by the upstream protein kinase RIPK3 is a hallmark of pathway activation, the precise checkpoints ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petrie EJ,Birkinshaw RW,Koide A,Denbaum E,Hildebrand JM,Garnish SE,Davies KA,Sandow JJ,Samson AL,Gavin X,Fitzgibbon C,Young SN,Hennessy PJ,Smith PPC,Webb AI,Czabotar PE,Koide S,Murphy JM

更新日期：2020-04-14 00:00:00

abstract：:Gene amplification may be visualized within a chromosome as a homogeneously stained region (HSR) and HSRs have rarely been reported in human tumor cells with identification of the amplified gene. A parental line and seven clones derived from KB cells resistant to methotrexate (MTX) contain dihydrofolate reductase (DHF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wolman SR,Craven ML,Grill SP,Domin BA,Cheng YC

更新日期：1983-02-01 00:00:00

abstract：:The lacY gene of Escherichia coli was cut into two approximately equal-size fragments with Afl II and subcloned individually or together under separate lac operator/promoters in plasmid pT7-5. Under these conditions, lac permease is expressed in two portions: (i) the N-terminal portion (the N terminus, the first six p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bibi E,Kaback HR

更新日期：1990-06-01 00:00:00

abstract：:The decrease with age of the adrenal-secreted dehydroepiandrosterone sulfate (DHEAS) in serum has suggested that it may be causally related to longevity. For the PAQUID [People (Personnes) Aged (Agées) About What (Quid, in Latin)] cohort of elderly subjects, we have previously reported higher DHEAS in men than in wome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mazat L,Lafont S,Berr C,Debuire B,Tessier JF,Dartigues JF,Baulieu EE

更新日期：2001-07-03 00:00:00

abstract：:Fibronectin mediates the adhesion of cells to collagen by first binding to the collagen substrate, followed by attachment of the cells to the fibronectin-collagen complex. Bovine brain gangliosides were found to block fibronectin-mediated cell adhesion to collagen in a concentration-dependent manner. The gangliosides ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kleinman HK,Martin GR,Fishman PH

更新日期：1979-07-01 00:00:00

abstract：:The gene encoding the receptor for hyaluronan-mediated motility (RHAMM) is overexpressed in many human cancers. However, it is unclear whether RHAMM plays a causal role in tumor initiation or progression. Using somatic gene transfer in a mouse model of islet cell tumorigenesis, we demonstrate that RHAMM isoform B (RHA...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Du YC,Chou CK,Klimstra DS,Varmus H

更新日期：2011-10-04 00:00:00

abstract：:The reduction in levels of the potentially toxic amyloid-beta peptide (Abeta) has emerged as one of the most important therapeutic goals in Alzheimer's disease. Key targets for this goal are factors that affect the expression and processing of the Abeta precursor protein (betaAPP). Earlier reports from our laboratory ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shaw KT,Utsuki T,Rogers J,Yu QS,Sambamurti K,Brossi A,Ge YW,Lahiri DK,Greig NH

更新日期：2001-06-19 00:00:00

abstract：:Receptors for platelet-derived growth factor (PDGF) have previously only been found on cells of mesenchymal and glial origin. This study shows PDGF receptors on an anaplastic thyroid carcinoma cell line, C 643, that was found to express thyroglobulin mRNA, confirming its origin from thyroid epithelium. Northern blot a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heldin NE,Gustavsson B,Claesson-Welsh L,Hammacher A,Mark J,Heldin CH,Westermark B

更新日期：1988-12-01 00:00:00

abstract：:This perspective addresses recent advances in lipid transport across the Gram-negative inner and outer membranes. While we include a summary of previously existing literature regarding this topic, we focus on the maintenance of lipid asymmetry (Mla) pathway. Discovered in 2009 by the Silhavy group [J. C. Malinverni, T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Powers MJ,Trent MS

更新日期：2019-08-27 00:00:00

abstract：:Many bacteria use acyl homoserine lactone signals to monitor cell density in a type of gene regulation termed quorum sensing and response. Synthesis of these signals is directed by homologs of the luxi gene of Vibrio fischeri. This communication resolves two critical issues concerning the synthesis of the V. fischeri ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaefer AL,Val DL,Hanzelka BL,Cronan JE Jr,Greenberg EP

更新日期：1996-09-03 00:00:00

abstract：:The ribonucleotide reductase system of Escherichia coli B participates in the biosynthesis of DNA by reducing ribonucleoside diphosphates to the corresponding deoxyribonucleotides. The enzyme is regulated in a complicated way by allosteric modifiers. We now find that infection of E. coli with the bacteriophage T4 resu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berglund O,Karlström O,Reichard P

更新日期：1969-03-01 00:00:00

abstract：:The brain disturbances that place a person at risk for developing depression are unknown. We imaged the brains of 131 individuals, ages 6 to 54 years, who were biological descendants (children or grandchildren) of individuals identified as having either moderate to severe, recurrent, and functionally debilitating depr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peterson BS,Warner V,Bansal R,Zhu H,Hao X,Liu J,Durkin K,Adams PB,Wickramaratne P,Weissman MM

更新日期：2009-04-14 00:00:00

abstract：:If climate change outpaces the rate of adaptive evolution within a site, populations previously well adapted to local conditions may decline or disappear, and banked seeds from those populations will be unsuitable for restoring them. However, if such adaptational lag has occurred, immigrants from historically warmer c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilczek AM,Cooper MD,Korves TM,Schmitt J

更新日期：2014-06-03 00:00:00

abstract：:Preincubation of quiescent BALB/C3T3 cells with uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) in conditioned medium resulted in a time- and concentration-dependent stimulation of uptake of 2-deoxyglucose, uridine, and alpha-aminoisobutyric acid over that seen in conditioned medium alone. The apparent Km values ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Natraj CV,Datta P

更新日期：1978-08-01 00:00:00