Phenserine regulates translation of beta -amyloid precursor protein mRNA by a putative interleukin-1 responsive element, a target for drug development.

abstract：:The biosynthesis of beta-nerve growth factor (betaNGF) was studied in mouse submaxillary glands incubated with L-[(35)S]cystine. betaNGF was isolated from tissue extracts by the addition of antiserum against betaNGF and the washed immunoprecipitates were analyzed by sodium dodecyl sulfate gel electrophoresis. With sho...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berger EA,Shooter EM

更新日期：1977-09-01 00:00:00

abstract：:We are interested in using recombinant adeno-associated viral vectors in the treatment of hemophilia A. Because of the size constraints of recombinant adeno-associated viral vectors, we delivered the heavy and light chains of the human factor 8 (hFVIII) cDNA independently by using two separate vectors. Recombinant AAV...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burton M,Nakai H,Colosi P,Cunningham J,Mitchell R,Couto L

更新日期：1999-10-26 00:00:00

abstract：:The GTPase-activating protein (GAP) has been postulated to function either as a negative regulator or as a possible target protein of Ras in mammalian cells and Xenopus oocytes. Ras must be localized in the plasma membrane of vertebrate cells to function, but GAP is localized in the cytosol. To test whether Ras functi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gibbs JB,Schaber MD,Schofield TL,Scolnick EM,Sigal IS

更新日期：1989-09-01 00:00:00

abstract：:Melanocyte stimulating hormone coupled to Sepharose effects an increase in tryosinase (EC 1.14.18.1; monophenol monoxygenase) activity of cultivated mouse melanoma cells. Synchronized cells are found to respond to melanocyte stimulating hormone only in the G2 phase of the cell cycle, although their response to cyclic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Varga JM,Dipasquale A,Pawelek J,McGuire JS,Lerner AB

更新日期：1974-05-01 00:00:00

abstract：:A readily accessible new class of near infrared (NIR) molecular probes has been synthesized and evaluated. Specific fluorophores in this unique xanthene based regioisomeric seminaphthofluorone dye series exhibit a combination of desirable characteristics including (i) low molecular weight (339 amu), (ii) aqueous solub...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yang Y,Lowry M,Xu X,Escobedo JO,Sibrian-Vazquez M,Wong L,Schowalter CM,Jensen TJ,Fronczek FR,Warner IM,Strongin RM

更新日期：2008-07-01 00:00:00

abstract：:We have developed an all-electronic digital microfluidic device for microscale chemical synthesis in organic solvents, operated by electrowetting-on-dielectric (EWOD). As an example of the principles, we demonstrate the multistep synthesis of [(18)F]FDG, the most common radiotracer for positron emission tomography (PE...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keng PY,Chen S,Ding H,Sadeghi S,Shah GJ,Dooraghi A,Phelps ME,Satyamurthy N,Chatziioannou AF,Kim CJ,van Dam RM

更新日期：2012-01-17 00:00:00

abstract：:Previous studies have established that incubation of low density lipoprotein (LDL) with cultured endothelial cells (EC) converts it to a new form (EC-modified LDL) that is now recognized by a specific receptor on macrophages (the acetyl LDL receptor) and is taken up and degraded 3-10 times more rapidly than native LDL...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Parthasarathy S,Steinbrecher UP,Barnett J,Witztum JL,Steinberg D

更新日期：1985-05-01 00:00:00

abstract：:An obstacle in the utilization of catalytic Abs for selective prodrug activation in cancer therapy has been systemic tumor targeting. Here we report the generation of catalytic Abs that effectively target tumor cells with undiminished prodrug activation capability. Ab conjugates were prepared by covalent conjugation o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abraham S,Guo F,Li LS,Rader C,Liu C,Barbas CF 3rd,Lerner RA,Sinha SC

更新日期：2007-03-27 00:00:00

abstract：:Sialic acid-recognizing Ig-like lectins (Siglecs) are signaling receptors that modulate immune responses, and are targeted for interactions by certain pathogens. We describe two primate Siglecs that were rendered nonfunctional by single genetic events during hominin evolution after our common ancestor with the chimpan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang X,Mitra N,Secundino I,Banda K,Cruz P,Padler-Karavani V,Verhagen A,Reid C,Lari M,Rizzi E,Balsamo C,Corti G,De Bellis G,Longo L,NISC Comparative Sequencing Program.,Beggs W,Caramelli D,Tishkoff SA,Hayakawa T,Gree

更新日期：2012-06-19 00:00:00

abstract：:Retinitis pigmentosa (RP) is an inherited neurodegenerative disease, in which the death of mutant rod photoreceptors leads secondarily to the non-cell autonomous death of cone photoreceptors. Gene therapy is a promising treatment strategy. Unfortunately, current methods of gene delivery treat only a fraction of diseas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koch SF,Duong JK,Hsu CW,Tsai YT,Lin CS,Wahl-Schott CA,Tsang SH

更新日期：2017-05-16 00:00:00

abstract：:An evolutionarily ancient plant hormone receptor complex comprising the α/β-fold hydrolase receptor KARRIKIN INSENSITIVE 2 (KAI2) and the F-box protein MORE AXILLARY GROWTH 2 (MAX2) mediates a range of developmental responses to smoke-derived butenolides called karrikins (KARs) and to yet elusive endogenous KAI2 ligan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carbonnel S,Das D,Varshney K,Kolodziej MC,Villaécija-Aguilar JA,Gutjahr C

更新日期：2020-09-01 00:00:00

abstract：:Communication about prescription drugs ought to be a paragon of public science communication. Unfortunately, it is not. Consumers see $4 billion of direct-to-consumer advertising annually, which typically fails to present data about how well drugs work. The professional label--the Food and Drug Administration's (FDA) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz LM,Woloshin S

更新日期：2013-08-20 00:00:00

abstract：:A predictive coarse-grained protein force field [associative memory, water-mediated, structure, and energy model for molecular dynamics (AWSEM)-MD] is used to study the energy landscapes and relative stabilities of amyloid-β protein (1-40) in the monomer and all of its oligomeric forms up to an octamer. We find that a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng W,Tsai MY,Chen M,Wolynes PG

更新日期：2016-10-18 00:00:00

abstract：:ARGONAUTE (AGO) RNA-binding proteins are involved in RNA silencing. They bind to short interfering RNAs (siRNAs) and microRNAs (miRNAs) through a conserved PAZ domain, and, in animals, they assemble into a multisubunit RNA-induced silencing complex (RISC). The mammalian AGO2, termed Slicer, directs siRNA- and miRNA-me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baumberger N,Baulcombe DC

更新日期：2005-08-16 00:00:00

abstract：:Swiftlets are small insectivorous birds, many of which nest in caves and are known to echolocate. Due to a lack of distinguishing morphological characters, the taxonomy of swiftlets is primarily based on the presence or absence of echolocating ability, together with nest characters. To test the reliability of these be...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee PL,Clayton DH,Griffiths R,Page RD

更新日期：1996-07-09 00:00:00

abstract：:Retrieval of recently acquired declarative memories depends on the hippocampus, but with time, retrieval is increasingly sustainable by neocortical representations alone. This process has been conceptualized as system-level consolidation. Using functional magnetic resonance imaging, we assessed over the course of thre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takashima A,Petersson KM,Rutters F,Tendolkar I,Jensen O,Zwarts MJ,McNaughton BL,Fernández G

更新日期：2006-01-17 00:00:00

abstract：:Brain serotonin (5-HT) deficiency and exposure to psychosocial stress have both been implicated in the etiology of depression and anxiety disorders, but whether 5-HT deficiency influences susceptibility to depression- and anxiety-like phenotypes induced by psychosocial stress has not been formally established. Most cl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sachs BD,Ni JR,Caron MG

更新日期：2015-02-24 00:00:00

abstract：:Art forgeries have existed since antiquity, but with the recent rapidly expanding commercialization of art, the approach to art authentication has demanded increasingly sophisticated detection schemes. So far, the most conclusive criterion in the field of counterfeit detection is the scientific proof of material anach...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hendriks L,Hajdas I,Ferreira ESB,Scherrer NC,Zumbühl S,Smith GD,Welte C,Wacker L,Synal HA,Günther D

更新日期：2019-07-02 00:00:00

abstract：:Hepatitis C virus (HCV) is a global epidemic manifested mainly by chronic infection. One strategy that HCV employs to establish chronic infection is to use the viral Ser protease NS3/4A to cleave some unknown cellular targets involved in innate immunity. Here we show that the target of NS3/4A is the mitochondrial anti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li XD,Sun L,Seth RB,Pineda G,Chen ZJ

更新日期：2005-12-06 00:00:00

abstract：:Mouse lymphocytic cells of the established line GF-14 were allowed to accumulate intracellular 3H-labeled aminoisobutyric acid (AIB), frozen, and stored over liquid N2. After internal radiation had reduced survival to 1 in 10(4), survivors were plated and tested for their ability to transport AIB. Out of 200 clones te...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finkelstein MC,Slayman CW,Adelberg EA

更新日期：1977-10-01 00:00:00

abstract：:We determined the frequency of integrated viral DNA in the livers of three woodchucks chronically infected with the woodchuck hepatitis virus before and during 30 weeks of therapy with the nucleoside analog L-FMAU [1-(2-fluoro-5-methyl-beta, L-arabinofuranosyl)uracil, clevudine]. We found that although viral covalentl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Summers J,Mason WS

更新日期：2004-01-13 00:00:00

abstract：:Although melanomas with mutant v-Raf murine sarcoma viral oncogene homolog B1 (BRAF) can now be effectively targeted, there is no molecular target for most melanomas expressing wild-type BRAF. Here, we show that the activation of Pleckstrin homology domain-interacting protein (PHIP), promotes melanoma metastasis, can ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： De Semir D,Nosrati M,Bezrookove V,Dar AA,Federman S,Bienvenu G,Venna S,Rangel J,Climent J,Meyer Tamgüney TM,Thummala S,Tong S,Leong SP,Haqq C,Billings P,Miller JR 3rd,Sagebiel RW,Debs R,Kashani-Sabet M

更新日期：2012-05-01 00:00:00

abstract：:Antisera directed against human dopamine beta-hydroxylase or serotonin were used to characterize the noradrenergic and serotonergic innervation patterns within the primary visual cortex of the squirrel monkey. The noradrenergic and serotonergic projections exhibit a high degree of laminar complementarity: layers V and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrison JH,Foote SL,Molliver ME,Bloom FE,Lidov HG

更新日期：1982-04-01 00:00:00

abstract：:A major challenge in DNA microarray analysis is to effectively dissociate actual gene expression values from experimental noise. We report here a detailed noise analysis for oligonuleotide-based microarray experiments involving reverse transcription, generation of labeled cRNA (target) through in vitro transcription, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tu Y,Stolovitzky G,Klein U

更新日期：2002-10-29 00:00:00

abstract：:Teratocarcinoma is a germ-line carcinoma giving rise to an embryoid tumor with structures derived from the three embryonic layers: mesoderm, endoderm, and ectoderm. Teratocarcinoma is widely used as an in vitro model system to study regulation of cell determination and differentiation during mammalian embryogenesis. M...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Trojan J,Johnson TR,Rudin SD,Blossey BK,Kelley KM,Shevelev A,Abdul-Karim FW,Anthony DD,Tykocinski ML,Ilan J

更新日期：1994-06-21 00:00:00

abstract：:Osteonectin is a prominent noncollagenous protein of developing bone. A 2150-base-pair cDNA coding for osteonectin, isolated from a bovine bone cell lambda gt11 expression library, was sequenced and identified by comparison with protein sequence data. The nucleotide sequence predicts that osteonectin contains 304 amin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bolander ME,Young MF,Fisher LW,Yamada Y,Termine JD

更新日期：1988-05-01 00:00:00

abstract：:Because synaptic vesicle exocytosis is a nano-mechanical process, it should be influenced by the mechanical properties of the cell membrane to which the vesicle fuses. By dissolving surfactants at various concentrations in the neuronal membrane, we have perturbed mechanical properties of the membrane and have found th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu Y,Stevens CF

更新日期：2008-11-18 00:00:00

abstract：:Recently, it has been reported that there is a differential subcellular distribution of components of the minor U12-dependent and major U2-dependent spliceosome, and further that the minor spliceosome functions in the cytoplasm. To study the subcellular localization of the snRNA components of both the major and minor ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pessa HK,Will CL,Meng X,Schneider C,Watkins NJ,Perälä N,Nymark M,Turunen JJ,Lührmann R,Frilander MJ

更新日期：2008-06-24 00:00:00

abstract：:Purified fractions of human apotransferrin, monoferric transferrins with iron on the acid-labile binding site and on the acid-stable binding site, and diferric transferrin have been prepared. The iron loading and unloading behavior of these preparations has been examined by isoelectric focusing. Iron release from the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huebers H,Josephson B,Huebers E,Csiba E,Finch C

更新日期：1981-04-01 00:00:00

abstract：:Nonribosomal peptides and polyketides are a diverse group of natural products with complex chemical structures and enormous pharmaceutical potential. They are synthesized on modular nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzyme complexes by a conserved thiotemplate mechanism. Here, we rep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang H,Fewer DP,Holm L,Rouhiainen L,Sivonen K

更新日期：2014-06-24 00:00:00