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A new ribonucleotide reductase system after infection with phage T4.

Abstract：

:The ribonucleotide reductase system of Escherichia coli B participates in the biosynthesis of DNA by reducing ribonucleoside diphosphates to the corresponding deoxyribonucleotides. The enzyme is regulated in a complicated way by allosteric modifiers. We now find that infection of E. coli with the bacteriophage T4 results in the appearance of a new ribonucleotide reductase system which shows a somewhat different pattern of regulation. Two new proteins, provisionally called fractions A and B, were purified from the extracts of infected bacteria. The reduction of ribonucleotides by these two fractions required the addition of either dithiothreitol or TPNH and E. coli thioredoxin reductase. Mutants of T4 which lacked fraction A activity were obtained. Fraction B may be a virus-induced thioredoxin.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Berglund O,Karlström O,Reichard P

doi

10.1073/pnas.62.3.829

subject

Has Abstract

pub_date

1969-03-01 00:00:00

pages

829-35

issue

3

eissn

0027-8424

issn

1091-6490

journal_volume

62

pub_type

杂志文章

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