当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > A region of the insulin receptor important for ligand binding (residues 450-601) is recognized by patients' autoimmune antibodies and inhibitory monoclonal antibodies.

A region of the insulin receptor important for ligand binding (residues 450-601) is recognized by patients' autoimmune antibodies and inhibitory monoclonal antibodies.

Abstract：

:Chimeric receptors containing different portions of the homologous human insulin receptor, insulin-like growth factor I receptor, and insulin receptor-related receptor were utilized to identify the epitopes recognized by various anti-insulin receptor antibodies. The antibodies studied included 12 monoclonal antibodies to the extracellular domain of the human insulin receptor as well as 15 patients' sera with autoimmune anti-insulin receptor antibodies. All of the patients' sera and all 8 monoclonal antibodies that inhibit insulin binding were found to recognize an epitope contained within residues 450-601 of the alpha subunit of the receptor. In contrast, 2 monoclonal antibodies that do not inhibit insulin binding were found to recognize the cysteine-rich region of the alpha subunit. Chimeric insulin receptors that had residues 450-601 replaced with the homologous residues of the insulin-like growth factor I receptor exhibited a decreased ability to bind insulin. In contrast, insulin-like growth factor I receptors that have had the comparable region replaced with that of the insulin receptor showed no decrease in their ability to bind ligand. These results indicate that residues 450-601 of the insulin receptor are important for insulin binding and include the major site for recognition by inhibitory monoclonal antibodies and patients' autoimmune anti-receptor antibodies.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Zhang B,Roth RA

doi

10.1073/pnas.88.21.9858

subject

Has Abstract

pub_date

1991-11-01 00:00:00

pages

9858-62

issue

21

eissn

0027-8424

issn

1091-6490

journal_volume

88

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Carbon starvation and growth rate-dependent regulation of the Escherichia coli ribosomal RNA promoters: differential control of dual promoters.

    abstract：:We studied the effects of carbon starvation and of varying the growth rate on the activity of each of the two tandem ribosomal RNA promoters from the rrnA operon of Escherichia coli. The cellular abundance of plasmid-encoded transcripts arising at promoters P1 and P2 and terminating at the ribosomal RNA terminator in ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.80.22.7010

    authors： Sarmientos P,Cashel M

    更新日期：1983-11-01 00:00:00

  • Growing optic nerve fibers follow neighbors during embryogenesis.

    abstract：:The embryonic development of the optic nerve of the zebrafish, Brachydanio rerio, was studied by three-dimensional computer reconstruction from serial section electron micrographs. Growing fibers from retinal ganglion cells had growth cones in contact with more mature fibers from adjacent cell bodies. In the observed ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.7.4374

    authors： Bodick N,Levinthal C

    更新日期：1980-07-01 00:00:00

  • From the Cover: Directed, efficient, and versatile modifications of the Drosophila genome by genomic engineering.

    abstract：:With the completion of genome sequences of major model organisms, increasingly sophisticated genetic tools are necessary for investigating the complex and coordinated functions of genes. Here we describe a genetic manipulation system termed "genomic engineering" in Drosophila. Genomic engineering is a 2-step process t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0900641106

    authors： Huang J,Zhou W,Dong W,Watson AM,Hong Y

    更新日期：2009-05-19 00:00:00

  • Full-length CD4 electroinserted in the erythrocyte membrane as a long-lived inhibitor of infection by human immunodeficiency virus.

    abstract：:Recombinant full-length CD4 expressed in Spodoptera frugiperda 9 cells with the baculovirus system was electroinserted in erythrocyte (RBC) membranes. Of the inserted CD4, 70% was "correctly" oriented as shown by fluorescence quenching experiments with fluorescein-labeled CD4. The inserted CD4 displayed the same epito...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.88.10.4409

    authors： Zeira M,Tosi PF,Mouneimne Y,Lazarte J,Sneed L,Volsky DJ,Nicolau C

    更新日期：1991-05-15 00:00:00

  • Assessment of normal and mutant human presenilin function in Caenorhabditis elegans.

    abstract：:We provide evidence that normal human presenilins can substitute for Caenorhabditis elegans SEL-12 protein in functional assays in vivo. In addition, six familial Alzheimer disease-linked mutant human presenilins were tested and found to have reduced ability to rescue the sel-12 mutant phenotype, suggesting that they ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.25.14940

    authors： Levitan D,Doyle TG,Brousseau D,Lee MK,Thinakaran G,Slunt HH,Sisodia SS,Greenwald I

    更新日期：1996-12-10 00:00:00

  • Purification and characterization of three types of protein kinase C from rabbit brain cytosol.

    abstract：:Three types of protein kinase C were purified from rabbit brain cytosol. Each type has a molecular mass of approximately 80 kDa and serves as a receptor for phorbol esters. Polyclonal antibodies produced to two protein kinase C types were relatively type-specific, indicating that these proteins have unique antigenic d...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.13.4418

    authors： Jaken S,Kiley SC

    更新日期：1987-07-01 00:00:00

  • Evidence for an ancient adaptive episode of convergent molecular evolution.

    abstract：:Documented cases of convergent molecular evolution due to selection are fairly unusual, and examples to date have involved only a few amino acid positions. However, because convergence mimics shared ancestry and is not accommodated by current phylogenetic methods, it can strongly mislead phylogenetic inference when it...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0900233106

    authors： Castoe TA,de Koning AP,Kim HM,Gu W,Noonan BP,Naylor G,Jiang ZJ,Parkinson CL,Pollock DD

    更新日期：2009-06-02 00:00:00

  • Lignin content in natural Populus variants affects sugar release.

    abstract：:The primary obstacle to producing renewable fuels from lignocellulosic biomass is a plant's recalcitrance to releasing sugars bound in the cell wall. From a sample set of wood cores representing 1,100 individual undomesticated Populus trichocarpa trees, 47 extreme phenotypes were selected across measured lignin conten...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1009252108

    authors： Studer MH,DeMartini JD,Davis MF,Sykes RW,Davison B,Keller M,Tuskan GA,Wyman CE

    更新日期：2011-04-12 00:00:00

  • Structure-guided enzymology of the lipid A acyltransferase LpxM reveals a dual activity mechanism.

    abstract：:Gram-negative bacteria possess a characteristic outer membrane, of which the lipid A constituent elicits a strong host immune response through the Toll-like receptor 4 complex, and acts as a component of the permeability barrier to prevent uptake of bactericidal compounds. Lipid A species comprise the bulk of the oute...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1610746113

    authors： Dovala D,Rath CM,Hu Q,Sawyer WS,Shia S,Elling RA,Knapp MS,Metzger LE 4th

    更新日期：2016-10-11 00:00:00

  • In vitro reconstitution of sortase-catalyzed pilus polymerization reveals structural elements involved in pilin cross-linking.

    abstract：:Covalently cross-linked pilus polymers displayed on the cell surface of Gram-positive bacteria are assembled by class C sortase enzymes. These pilus-specific transpeptidases located on the bacterial membrane catalyze a two-step protein ligation reaction, first cleaving the LPXTG motif of one pilin protomer to form an ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1800954115

    authors： Chang C,Amer BR,Osipiuk J,McConnell SA,Huang IH,Hsieh V,Fu J,Nguyen HH,Muroski J,Flores E,Ogorzalek Loo RR,Loo JA,Putkey JA,Joachimiak A,Das A,Clubb RT,Ton-That H

    更新日期：2018-06-12 00:00:00

  • The first day of the Cenozoic.

    abstract：:Highly expanded Cretaceous-Paleogene (K-Pg) boundary section from the Chicxulub peak ring, recovered by International Ocean Discovery Program (IODP)-International Continental Scientific Drilling Program (ICDP) Expedition 364, provides an unprecedented window into the immediate aftermath of the impact. Site M0077 inclu...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1909479116

    authors： Gulick SPS,Bralower TJ,Ormö J,Hall B,Grice K,Schaefer B,Lyons S,Freeman KH,Morgan JV,Artemieva N,Kaskes P,de Graaff SJ,Whalen MT,Collins GS,Tikoo SM,Verhagen C,Christeson GL,Claeys P,Coolen MJL,Goderis S,Goto K,

    更新日期：2019-09-24 00:00:00

  • Sphingosine-1-phosphate receptor 1 activation in astrocytes contributes to neuropathic pain.

    abstract：:Neuropathic pain afflicts millions of individuals and represents a major health problem for which there is limited effective and safe therapy. Emerging literature links altered sphingolipid metabolism to nociceptive processing. However, the neuropharmacology of sphingolipid signaling in the central nervous system in t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1820466116

    authors： Chen Z,Doyle TM,Luongo L,Largent-Milnes TM,Giancotti LA,Kolar G,Squillace S,Boccella S,Walker JK,Pendleton A,Spiegel S,Neumann WL,Vanderah TW,Salvemini D

    更新日期：2019-05-21 00:00:00

  • Chromatin conformation remains stable upon extensive transcriptional changes driven by heat shock.

    abstract：:Heat shock (HS) initiates rapid, extensive, and evolutionarily conserved changes in transcription that are accompanied by chromatin decondensation and nucleosome loss at HS loci. Here we have employed in situ Hi-C to determine how heat stress affects long-range chromatin conformation in human and Drosophila cells. We ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1901244116

    authors： Ray J,Munn PR,Vihervaara A,Lewis JJ,Ozer A,Danko CG,Lis JT

    更新日期：2019-09-24 00:00:00

  • Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity.

    abstract：:The exquisite selectivity and catalytic activity of enzymes have been shaped by the effects of positive and negative selection pressure during the course of evolution. In contrast, enzyme variants engineered by using in vitro screening techniques to accept novel substrates typically display a higher degree of catalyti...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0500063102

    authors： Varadarajan N,Gam J,Olsen MJ,Georgiou G,Iverson BL

    更新日期：2005-05-10 00:00:00

  • Partly native epitopes are already present on early intermediates in the folding of tryptophan synthase.

    abstract：:Two monoclonal antibodies directed against the native beta 2 subunit of Escherichia coli tryptophan synthase [L-serine hydro-lyase (adding indoleglycerol-phosphate), EC 4.2.1.20], one recognizing the C-terminal F1 domain and the other the N-terminal F2 domain, were used to detect immunoreactive intermediates in the fo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.5.1147

    authors： Blond S,Goldberg M

    更新日期：1987-03-01 00:00:00

  • Foxg1 localizes to mitochondria and coordinates cell differentiation and bioenergetics.

    abstract：:Forkhead box g1 (Foxg1) is a nuclear-cytosolic transcription factor essential for the forebrain development and involved in neurodevelopmental and cancer pathologies. Despite the importance of this protein, little is known about the modalities by which it exerts such a large number of cellular functions. Here we show ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1515190112

    authors： Pancrazi L,Di Benedetto G,Colombaioni L,Della Sala G,Testa G,Olimpico F,Reyes A,Zeviani M,Pozzan T,Costa M

    更新日期：2015-11-10 00:00:00

  • Cross-cultural recognition of basic emotions through nonverbal emotional vocalizations.

    abstract：:Emotional signals are crucial for sharing important information, with conspecifics, for example, to warn humans of danger. Humans use a range of different cues to communicate to others how they feel, including facial, vocal, and gestural signals. We examined the recognition of nonverbal emotional vocalizations, such a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0908239106

    authors： Sauter DA,Eisner F,Ekman P,Scott SK

    更新日期：2010-02-09 00:00:00

  • Ultraviolet-stimulated DNA synthesis in toluenzied Escherichia coli deficient in DNA polymerase I.

    abstract：:Ultraviolet (UV)-stimulated nonconservative DNA synthesis has been observed in E. coli rendered permeable to nucleoside triphosphates by exposure to toluene. This synthesis is detected in toluenized cells in which the background of UV-independent DNA synthesis is reduced by use of dnaB mutants temperature-sensitive fo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.70.1.129

    authors： Masker WE,Hanawalt PC

    更新日期：1973-01-01 00:00:00

  • Resistance-associated point mutations in insecticide-insensitive acetylcholinesterase.

    abstract：:Extensive utilization of pesticides against insects provides us with a good model for studying the adaptation of a eukaryotic genome to a strong selective pressure. One mechanism of resistance is the alteration of acetylcholinesterase (EC 3.1.1.7), the molecular target for organophosphates and carbamates. Here, we rep...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.13.5922

    authors： Mutero A,Pralavorio M,Bride JM,Fournier D

    更新日期：1994-06-21 00:00:00

  • Plant mating system transitions drive the macroevolution of defense strategies.

    abstract：:Understanding the factors that shape macroevolutionary patterns in functional traits is a central goal of evolutionary biology. Alternative strategies of sexual reproduction (inbreeding vs. outcrossing) have divergent effects on population genetic structure and could thereby broadly influence trait evolution. However,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1213867110

    authors： Campbell SA,Kessler A

    更新日期：2013-03-05 00:00:00

  • Structural basis for gating mechanisms of a eukaryotic P-glycoprotein homolog.

    abstract：:P-glycoprotein is an ATP-binding cassette multidrug transporter that actively transports chemically diverse substrates across the lipid bilayer. The precise molecular mechanism underlying transport is not fully understood. Here, we present crystal structures of a eukaryotic P-glycoprotein homolog, CmABCB1 from Cyanidi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1321562111

    authors： Kodan A,Yamaguchi T,Nakatsu T,Sakiyama K,Hipolito CJ,Fujioka A,Hirokane R,Ikeguchi K,Watanabe B,Hiratake J,Kimura Y,Suga H,Ueda K,Kato H

    更新日期：2014-03-18 00:00:00

  • Synthesis of adenosine triphosphate in respiration-inhibited submitochondrial particles induced by microsecond electric pulses.

    abstract：:Phosphorylation of ADP to ATP was induced in nonrespiring submitochondrial particles (SMP) from rat liver by the application of electric pulses with field strengths of 10-35 kV/cm and a decay time of 60 mus. In all cases respiration was inhibited completely by using cyanide or rotenone. Newly formed ATP was measured b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.12.7473

    authors： Teissie J,Knox BE,Tsong TY,Wehrle J

    更新日期：1981-12-01 00:00:00

  • Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography.

    abstract：:Activation of G protein-coupled receptors (GPCRs) is triggered and regulated by structural rearrangement of the transmembrane heptahelical bundle containing a number of highly conserved residues. In rhodopsin, a prototypical GPCR, the helical bundle accommodates an intrinsic inverse-agonist 11-cis-retinal, which under...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.082666399

    authors： Okada T,Fujiyoshi Y,Silow M,Navarro J,Landau EM,Shichida Y

    更新日期：2002-04-30 00:00:00

  • Adenosine diphosphate glucose pyrophosphatase: A plastidial phosphodiesterase that prevents starch biosynthesis.

    abstract：:A distinct phosphodiesterasic activity (EC 3.1.4) was found in both mono- and dicotyledonous plants that catalyzes the hydrolytic breakdown of ADPglucose (ADPG) to produce equimolar amounts of glucose-1-phosphate and AMP. The enzyme responsible for this activity, referred to as ADPG pyrophosphatase (AGPPase), was puri...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.120168097

    authors： Rodriguez-López M,Baroja-Fernández E,Zandueta-Criado A,Pozueta-Romero J

    更新日期：2000-07-18 00:00:00

  • Transdermal cold atmospheric plasma-mediated immune checkpoint blockade therapy.

    abstract：:Despite the promise of immune checkpoint blockade (ICB) therapy against cancer, challenges associated with low objective response rates and severe systemic side effects still remain and limit its clinical applications. Here, we described a cold atmospheric plasma (CAP)-mediated ICB therapy integrated with microneedles...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1917891117

    authors： Chen G,Chen Z,Wen D,Wang Z,Li H,Zeng Y,Dotti G,Wirz RE,Gu Z

    更新日期：2020-02-18 00:00:00

  • Patterned deposition of cells and proteins onto surfaces by using three-dimensional microfluidic systems.

    abstract：:Three-dimensional microfluidic systems were fabricated and used to pattern proteins and mammalian cells on a planar substrate. The three-dimensional topology of the microfluidic network in the stamp makes this technique a versatile one with which to pattern multiple types of proteins and cells in complex, discontinuou...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.040562297

    authors： Chiu DT,Jeon NL,Huang S,Kane RS,Wargo CJ,Choi IS,Ingber DE,Whitesides GM

    更新日期：2000-03-14 00:00:00

  • Separation of functional subsets of human T cells by a monoclonal antibody.

    abstract：:A monoclonal antibody was produced to human peripheral blood T cells. This hybridoma antibody, termed OKT4, was reactive by indirect immunofluorescence with only 55-60% of the peripheral blood T cell population (OKT4+) and unreactive with normal B cells, null cells, and macrophages. The OKT4- T cell population contain...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.8.4061

    authors： Reinherz EL,Kung PC,Goldstein G,Schlossman SF

    更新日期：1979-08-01 00:00:00

  • Escherichia coli rho factor induces release of yeast RNA polymerase II but not polymerase I or III.

    abstract：:Purified RNA polymerase II (pol II) from the yeast Saccharomyces cerevisiae pauses without releasing at many locations during in vitro transcription. Pausing can be induced by intrinsic DNA sequence as well as by specific DNA bound proteins such as the RNA pol I termination factor, Reb1p, or lac repressor. Addition of...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.9.4900

    authors： Lang WH,Platt T,Reeder RH

    更新日期：1998-04-28 00:00:00

  • Evidence that DNA is present in abnormal tubulofilamentous structures found in scrapie.

    abstract：:Abnormal tubulofilamentous structures have been identified in electron micrographs of thin sections and negatively stained impression grids prepared from brains of animals with scrapie and other spongiform encephalopathies, and we showed that such tubules contain a core of filamentous structures resembling scrapie-ass...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.10.3575

    authors： Narang HK,Asher DM,Gajdusek DC

    更新日期：1988-05-01 00:00:00

  • Isolation of immortal cell lines from the first stage of murine leukemia virus-induced leukemia.

    abstract：:Friend murine leukemia virus (F-MuLV) is a replication-competent retrovirus that induces a rapidly fetal leukemia in susceptible mice (stage I disease). Leukemia cells obtained from these animals do not grow in cell culture using standard tissue culture conditions. However, in the presence of WEHI-3 cell-conditioned m...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.81.17.5464

    authors： Oliff A,Oliff I,Schmidt B,Famulari N

    更新日期：1984-09-01 00:00:00