Foxg1 localizes to mitochondria and coordinates cell differentiation and bioenergetics.

Abstract:

:Forkhead box g1 (Foxg1) is a nuclear-cytosolic transcription factor essential for the forebrain development and involved in neurodevelopmental and cancer pathologies. Despite the importance of this protein, little is known about the modalities by which it exerts such a large number of cellular functions. Here we show that a fraction of Foxg1 is localized within the mitochondria in cell lines, primary neuronal or glial cell cultures, and in the mouse cortex. Import of Foxg1 in isolated mitochondria appears to be membrane potential-dependent. Amino acids (aa) 277-302 were identified as critical for mitochondrial localization. Overexpression of full-length Foxg1 enhanced mitochondrial membrane potential (ΔΨm) and promoted mitochondrial fission and mitosis. Conversely, overexpression of the C-term Foxg1 (aa 272-481), which is selectively localized in the mitochondrial matrix, enhanced organelle fusion and promoted the early phase of neuronal differentiation. These findings suggest that the different subcellular localizations of Foxg1 control the machinery that brings about cell differentiation, replication, and bioenergetics, possibly linking mitochondrial functions to embryonic development and pathological conditions.

authors

Pancrazi L,Di Benedetto G,Colombaioni L,Della Sala G,Testa G,Olimpico F,Reyes A,Zeviani M,Pozzan T,Costa M

doi

10.1073/pnas.1515190112

subject

Has Abstract

pub_date

2015-11-10 00:00:00

pages

13910-5

issue

45

eissn

0027-8424

issn

1091-6490

pii

1515190112

journal_volume

112

pub_type

杂志文章
  • Phosphorescence/microwave double-resonance spectra of tryptophan perturbed by methylmercury(II).

    abstract::Amplitude-modulated phosphorescence/microwave double-resonance (AM-PMDR) spectra are reported for complexes of methylmercury(II) cation, designated CH3Hg(II), with tryptophan and glyceraldehyde-3-phosphate dehydrogenase (GPDHase; from rabbit muscle). Wavelength shifts are observed in the AM-PMDR spectra of CH3Hg(II)-t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.79.14.4313

    authors: Davis JM,Maki AH

    更新日期:1982-07-01 00:00:00

  • LXRs control lipid-inducible expression of the apolipoprotein E gene in macrophages and adipocytes.

    abstract::Apolipoprotein E (apoE) secreted by macrophages in the artery wall exerts an important protective effect against the development of atherosclerosis, presumably through its ability to promote lipid efflux. Previous studies have shown that increases in cellular free cholesterol levels stimulate apoE transcription in mac...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.021488798

    authors: Laffitte BA,Repa JJ,Joseph SB,Wilpitz DC,Kast HR,Mangelsdorf DJ,Tontonoz P

    更新日期:2001-01-16 00:00:00

  • Elevated serum ribonuclease in patients with pancreatic cancer.

    abstract::Serum RNase (ribonuclease) of normal persons and of patients with pancreatitis, carcinoma of pancreas, or other neoplasms was determined with poly(C) as substrate. Strikingly abnormal elevations occur in the serum RNase of patients with pancreatic cancer. There is no elevation in the serum RNase level of patients with...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.73.7.2308

    authors: Reddi KK,Holland JF

    更新日期:1976-07-01 00:00:00

  • On the mechanism of actomyosin ATPase from fast muscle.

    abstract::The labeled inorganic phosphate formed by enzymatic hydrolysis of [gamma-18O]ATP in normal water was derivatized to trimethyl phosphate and analyzed for the proportions of [18O3]Pi, [18O2]Pi, [18O1]Pi, and [18O0]Pi. The proportions observed were correlated with the kinetics of intermediate exchange by using a kinetic ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.4.2067

    authors: Midelfort CF

    更新日期:1981-04-01 00:00:00

  • Nuclear receptor RORα regulates pathologic retinal angiogenesis by modulating SOCS3-dependent inflammation.

    abstract::Pathologic ocular angiogenesis is a leading cause of blindness, influenced by both dysregulated lipid metabolism and inflammation. Retinoic-acid-receptor-related orphan receptor alpha (RORα) is a lipid-sensing nuclear receptor with diverse biologic function including regulation of lipid metabolism and inflammation; ho...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1504387112

    authors: Sun Y,Liu CH,SanGiovanni JP,Evans LP,Tian KT,Zhang B,Stahl A,Pu WT,Kamenecka TM,Solt LA,Chen J

    更新日期:2015-08-18 00:00:00

  • Genomewide transcriptional changes associated with genetic alterations and nutritional supplementation affecting tryptophan metabolism in Bacillus subtilis.

    abstract::DNA microarrays comprising approximately 95% of the Bacillus subtilis annotated protein coding ORFs were deployed to generate a series of snapshots of genomewide transcriptional changes that occur when cells are grown under various conditions that are expected to increase or decrease transcription of the trp operon se...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1031606100

    authors: Berka RM,Cui X,Yanofsky C

    更新日期:2003-05-13 00:00:00

  • Similar DNA recognition properties of alternatively spliced Drosophila POU factors.

    abstract::The POU-IV or "Brn-3" class of POU-domain transcription factors is represented in Drosophila by I-POU and twin-of-I-POU, alternative splice products of the I-POU gene. I-POU has been previously reported to inhibit DNA binding by the POU-III class factor drifter/Cf1a via the formation of heterodimeric complexes. Here w...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.26.15097

    authors: Turner EE

    更新日期:1996-12-24 00:00:00

  • Mapping activation and repression domains of the vnd/NK-2 homeodomain protein.

    abstract::A transient transfection assay using Drosophila S2 tissue culture cells and WT and mutant Drosophila vnd/NK-2 homeobox cDNAs was used to localize repression and activation domains of vnd/NK-2 homeodomain protein. A repression domain was identified near the N terminus of vnd/NK-2 homeodomain protein (amino acid residue...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404775101

    authors: Stepchenko A,Nirenberg M

    更新日期:2004-09-07 00:00:00

  • Cultivation of a human-associated TM7 phylotype reveals a reduced genome and epibiotic parasitic lifestyle.

    abstract::The candidate phylum TM7 is globally distributed and often associated with human inflammatory mucosal diseases. Despite its prevalence, the TM7 phylum remains recalcitrant to cultivation, making it one of the most enigmatic phyla known. In this study, we cultivated a TM7 phylotype (TM7x) from the human oral cavity. Th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1419038112

    authors: He X,McLean JS,Edlund A,Yooseph S,Hall AP,Liu SY,Dorrestein PC,Esquenazi E,Hunter RC,Cheng G,Nelson KE,Lux R,Shi W

    更新日期:2015-01-06 00:00:00

  • Base insertion and deletion mutations induced in an Escherichia coli plasmid by benzo[a]pyrene-7,8-dihydrodiol-9,10-epoxide.

    abstract::Mutations induced by (+/-)trans-benzo-[a]pyrene-7,8-dihydrodiol-9,10-epoxide (BaP-diol epoxide-1) were selected by using a recombinant plasmid vector system designed for the study of transcription termination signals. The plasmid contains a transcription terminator positioned between a promotor signal and the Escheric...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.11.6817

    authors: Mizusawa H,Lee CH,Kakefuda T,McKenney K,Shimatake H,Rosenberg M

    更新日期:1981-11-01 00:00:00

  • Simultaneous fluorescence-activated cell sorter analysis of two distinct transcriptional elements within a single cell using engineered green fluorescent proteins.

    abstract::Green fluorescent protein (GFP) is widely used as a reporter gene in both prokaryotes and eukaryotes. However, the fluorescence levels of wild-type GFP (wtGFP) are not bright enough for fluorescence-activated cell sorting or flow cytometry. Several GFP variants were generated that are brighter or have altered excitati...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.16.8508

    authors: Anderson MT,Tjioe IM,Lorincz MC,Parks DR,Herzenberg LA,Nolan GP,Herzenberg LA

    更新日期:1996-08-06 00:00:00

  • Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant.

    abstract::The lipid monogalactosyl diacylglycerol (MGD) is a major structural component of photosynthetic membranes in chloroplasts. Its formation is catalyzed by the enzyme MGD synthase. In many plants, MGD derives from two different biosynthetic pathways: the prokaryotic pathway, which operates entirely within the plastid, an...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.100132197

    authors: Jarvis P,Dörmann P,Peto CA,Lutes J,Benning C,Chory J

    更新日期:2000-07-05 00:00:00

  • Ordered upwind methods for static Hamilton-Jacobi equations.

    abstract::We introduce a family of fast ordered upwind methods for approximating solutions to a wide class of static Hamilton-Jacobi equations with Dirichlet boundary conditions. Standard techniques often rely on iteration to converge to the solution of a discretized version of the partial differential equation. Our fast method...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.201222998

    authors: Sethian JA,Vladimirsky A

    更新日期:2001-09-25 00:00:00

  • Searching for missing heritability: designing rare variant association studies.

    abstract::Genetic studies have revealed thousands of loci predisposing to hundreds of human diseases and traits, revealing important biological pathways and defining novel therapeutic hypotheses. However, the genes discovered to date typically explain less than half of the apparent heritability. Because efforts have largely foc...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1322563111

    authors: Zuk O,Schaffner SF,Samocha K,Do R,Hechter E,Kathiresan S,Daly MJ,Neale BM,Sunyaev SR,Lander ES

    更新日期:2014-01-28 00:00:00

  • Gene control of tyrosine kinase TIE2 and vascular manifestations of infections.

    abstract::Ligands of the endothelial-enriched tunica interna endothelial cell kinase 2 (Tie2) are markedly imbalanced in severe infections associated with vascular leakage, yet regulation of the receptor itself has been understudied in this context. Here, we show that TIE2 gene expression may constitute a novel vascular barrier...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1519467113

    authors: Ghosh CC,David S,Zhang R,Berghelli A,Milam K,Higgins SJ,Hunter J,Mukherjee A,Wei Y,Tran M,Suber F,Kobzik L,Kain KC,Lu S,Santel A,Yano K,Guha P,Dumont DJ,Christiani DC,Parikh SM

    更新日期:2016-03-01 00:00:00

  • Recent warming by latitude associated with increased length of ragweed pollen season in central North America.

    abstract::A fundamental aspect of climate change is the potential shifts in flowering phenology and pollen initiation associated with milder winters and warmer seasonal air temperature. Earlier floral anthesis has been suggested, in turn, to have a role in human disease by increasing time of exposure to pollen that causes aller...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1014107108

    authors: Ziska L,Knowlton K,Rogers C,Dalan D,Tierney N,Elder MA,Filley W,Shropshire J,Ford LB,Hedberg C,Fleetwood P,Hovanky KT,Kavanaugh T,Fulford G,Vrtis RF,Patz JA,Portnoy J,Coates F,Bielory L,Frenz D

    更新日期:2011-03-08 00:00:00

  • Recombinational repair of gaps in DNA is asymmetric in Ustilago maydis and can be explained by a migrating D-loop model.

    abstract::Recombinational repair of double-stranded DNA gaps was investigated in Ustilago maydis. The experimental system was designed for analysis of repair of an autonomously replicating plasmid containing a cloned gene disabled by an internal deletion. It was discovered that crossing over rarely accompanied gap repair. The s...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.11.5419

    authors: Ferguson DO,Holloman WK

    更新日期:1996-05-28 00:00:00

  • Alcohol dehydrogenase gene of Drosophila melanogaster: relationship of intervening sequences to functional domains in the protein.

    abstract::The gene that codes for Drosophila alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase EC 1.1.1.1) was identified in a bacteriophage lambda library of genomic Drosophila DNA by using ADH cDNA cloned DNA as a probe. The DNA sequence of the protein encoding region was shown to be in agreement with the amino acid seq...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.5.2717

    authors: Benyajati C,Place AR,Powers DA,Sofer W

    更新日期:1981-05-01 00:00:00

  • Heparin- and sulfatide-binding peptides from the type I repeats of human thrombospondin promote melanoma cell adhesion.

    abstract::Peptides from the three type I repeats of human endothelial cell thrombospondin, containing the consensus sequence-Trp-Ser-Xaa-Trp-, bind to sulfated glycoconjugates including heparin and sulfatide. The peptides are potent inhibitors for the binding of thrombospondin, laminin, or apolipoprotein E to these ligands. The...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.7.3040

    authors: Guo NH,Krutzsch HC,Nègre E,Vogel T,Blake DA,Roberts DD

    更新日期:1992-04-01 00:00:00

  • HSP90 is a chaperone for DLK and is required for axon injury signaling.

    abstract::Peripheral nerve injury induces a robust proregenerative program that drives axon regeneration. While many regeneration-associated genes are known, the mechanisms by which injury activates them are less well-understood. To identify such mechanisms, we performed a loss-of-function pharmacological screen in cultured adu...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1805351115

    authors: Karney-Grobe S,Russo A,Frey E,Milbrandt J,DiAntonio A

    更新日期:2018-10-16 00:00:00

  • Herpes simplex virus glycoproteins gB and gH function in fusion between the virion envelope and the outer nuclear membrane.

    abstract::Herpesviruses must traverse the nuclear envelope to gain access to the cytoplasm and, ultimately, to exit cells. It is believed that herpesvirus nucleocapsids enter the perinuclear space by budding through the inner nuclear membrane (NM). To reach the cytoplasm these enveloped particles must fuse with the outer NM and...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0703790104

    authors: Farnsworth A,Wisner TW,Webb M,Roller R,Cohen G,Eisenberg R,Johnson DC

    更新日期:2007-06-12 00:00:00

  • Obesity-linked regulation of the adipsin gene promoter in transgenic mice.

    abstract::The mouse adipsin gene encodes a member of the serine protease family that is expressed predominantly in adipose tissue and is secreted into the bloodstream. Adipsin expression is sharply down-regulated in several models of genetic and acquired obesity, representing the first example of an adipocyte gene whose express...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.19.7490

    authors: Platt KA,Min HY,Ross SR,Spiegelman BM

    更新日期:1989-10-01 00:00:00

  • Inhibition of human immunodeficiency virus type 1 expression by a hairpin ribozyme.

    abstract::Ribozymes are RNAs that possess the dual properties of RNA sequence-specific recognition, analogous to conventional antisense molecules, and RNA substrate destruction via site-specific cleavage. The cleavage reaction is catalytic in that more than one substrate molecule is processed per ribozyme molecule. We have desi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.22.10802

    authors: Ojwang JO,Hampel A,Looney DJ,Wong-Staal F,Rappaport J

    更新日期:1992-11-15 00:00:00

  • Deuterium nuclear magnetic resonance investigation of the effects of proteins and polypeptides on hydrocarbon chain order in model membrane systems.

    abstract::Deuterium Fourier-transform nuclear magnetic resonance spectra have been obtained of 1-myristoyl 2-(14,14,14-trideutero)myristoyl phosphatidylcholine bilayers at 34.1 MHz by using the quadrupole echo pulse technique. Thereby, we have investigated the effects upon the deuterated dimyristoyl phosphatidylcholine bilayers...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.75.10.4657

    authors: Oldfield E,Gilmore R,Glaser M,Gutowsky HS,Hshung JC,Kang SY,King TE,Meadows M,Rice D

    更新日期:1978-10-01 00:00:00

  • The nonequilibrium mechanism for ultrasensitivity in a biological switch: sensing by Maxwell's demons.

    abstract::The Escherichia coli flagellar motor senses the intracellular concentration of the response regulator CheY-P and responds by varying the bias between its counterclockwise (CCW) and clockwise (CW) rotational states. The response is ultrasensitive with a large Hill coefficient (approximately 10). Recently, the detailed ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0804641105

    authors: Tu Y

    更新日期:2008-08-19 00:00:00

  • Sodium-coupled glycine uptake by Ehrlich ascites tumor cells results in an increase in cell volume and plasma membrane channel activities.

    abstract::The addition of 10 mM glycine to a physiological saline bathing Ehrlich ascites tumor cells is followed by a slow increase in cell volume that plateaus between 15 and 30 min at a level approximately equal to 17% greater than the control volume; this increase is not observed when glycine is added to cells suspended in ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.1.279

    authors: Hudson RL,Schultz SG

    更新日期:1988-01-01 00:00:00

  • Peroxynitrite inhibition of Coxsackievirus infection by prevention of viral RNA entry.

    abstract::Although peroxynitrite is harmful to the host, the beneficial effects of peroxynitrite are less well understood. We explored the role of peroxynitrite in the host immune response to Coxsackievirus infection. Peroxynitrite inhibits viral replication in vitro, in part by inhibiting viral RNA entry into the host cell. Ni...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0400518101

    authors: Padalko E,Ohnishi T,Matsushita K,Sun H,Fox-Talbot K,Bao C,Baldwin WM 3rd,Lowenstein CJ

    更新日期:2004-08-10 00:00:00

  • Chromatographically isolated CD63+CD81+ extracellular vesicles from mesenchymal stromal cells rescue cognitive impairments after TBI.

    abstract::Extracellular vesicles (EVs) secreted by cells present an attractive strategy for developing new therapies, but progress in the field is limited by several issues: The quality of the EVs varies with the type and physiological status of the producer cells; protocols used to isolate the EVs are difficult to scale up; an...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1522297113

    authors: Kim DK,Nishida H,An SY,Shetty AK,Bartosh TJ,Prockop DJ

    更新日期:2016-01-05 00:00:00

  • Antigen induces chronic idiotype suppression.

    abstract::Hapten coupled to isologous gamma globulin can substitute for anti-idiotypic antibody in the induction of neonatal chronic idiotype suppression. The immunologically suppressed animals fail to express an idiotypically defined subset of anti-hapten antibodies in later life and harbor regulatory T cells that prevent the ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.12.4217

    authors: Tokuhisa T,Rajewsky K

    更新日期:1985-06-01 00:00:00

  • Chemical synthesis and X-ray structure of a heterochiral {D-protein antagonist plus vascular endothelial growth factor} protein complex by racemic crystallography.

    abstract::Total chemical synthesis was used to prepare the mirror image (D-protein) form of the angiogenic protein vascular endothelial growth factor (VEGF-A). Phage display against D-VEGF-A was used to screen designed libraries based on a unique small protein scaffold in order to identify a high affinity ligand. Chemically syn...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1210483109

    authors: Mandal K,Uppalapati M,Ault-Riché D,Kenney J,Lowitz J,Sidhu SS,Kent SB

    更新日期:2012-09-11 00:00:00