Crystal structures of native and thrombin-complexed heparin cofactor II reveal a multistep allosteric mechanism.

abstract：:Bacteriorhodopsin from Halobacterium halobium has been solubilized in the nonionic detergent Triton X-100. The circular dichroic spectrum and hydrodynamic properties indicate that the structure of this protein in the detergent is not significantly altered from that of the native membrane-bound form. Bacteriorhodopsin ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reyenolds JA,Stoeckenius W

更新日期：1977-07-01 00:00:00

abstract：:pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli. Fragment A (41.7 kilobases), but not fragment B (14.4 kilobases), can be cloned by insertion into the unrelated plasmids mini-F and ColE1. Fragment B...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Figurski DH,Helinski DR

更新日期：1979-04-01 00:00:00

abstract：:Despite the ubiquitous use of phosphoramidite chemistry in the synthesis of biophosphates, catalytic asymmetric phosphoramidite transfer remains largely unexplored for phosphate ester synthesis. We have discovered that a tetrazole-functionalized peptide, in the presence of 10-Å molecular sieves, functions as an enanti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jordan PA,Kayser-Bricker KJ,Miller SJ

更新日期：2010-11-30 00:00:00

abstract：:R. A. Fisher's 1930 "reproductive value" is defined as the contribution made by a population's initial age elements to its asymptotically dominating exponential growth mode. For the Leslie discrete-time model, it is the characteristic row vector of the Leslie matrix, and for the integral-equation model of Lotka the si...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samuelson PA

更新日期：1977-11-01 00:00:00

abstract：:The circular dichroism of hypothetical, water-filled, spherical shells, 75-3500 nm in radius, with walls 7.5 nm thick, composed of poly(L-lysine) in various conformational proportions, and suspended in water, were computed from the known optical properties of this polypeptide by classical general light-scattering theo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallach DF,Low DA,Bertland AV

更新日期：1973-11-01 00:00:00

abstract：:In classical knot theory and the theory of quantum invariants substantial effort was directed toward the search for unknotting moves on links. We solve, in this article, several classical problems concerning unknotting moves. Our approach uses a concept, Burnside groups of links, that establishes an unexpected relatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dabkowski MK,Przytycki JH

更新日期：2004-12-14 00:00:00

abstract：:Neuropeptide B (NPB) and neuropeptide W (NPW) are endogenous neuropeptide ligands for the G protein-coupled receptors NPBWR1 and NPBWR2. Here we report that the majority of NPW neurons in the mesolimbic region possess tyrosine hydroxylase immunoreactivity, indicating that a small subset of dopaminergic neurons coexpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Motoike T,Long JM,Tanaka H,Sinton CM,Skach A,Williams SC,Hammer RE,Sakurai T,Yanagisawa M

更新日期：2016-05-24 00:00:00

abstract：:Cancer patients and tumor-bearing mice possess serum antibodies that recognize antigens expressed by cancer cells at the time of diagnosis. After diagnosis, cancers progress to more aggressive stages, most often by acquiring new genetic changes that can give rise to new proteins, some of which are antigenic. However, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spiotto MT,Reth MA,Schreiber H

更新日期：2003-04-29 00:00:00

abstract：:Protein-DNA interactions were studied in vivo at the region containing a human DNA replication origin, located at the 3' end of the lamin B2 gene and partially overlapping the promoter of another gene, located downstream. DNase I treatment of nuclei isolated from both exponentially growing and nonproliferating HL-60 c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dimitrova DS,Giacca M,Demarchi F,Biamonti G,Riva S,Falaschi A

更新日期：1996-02-20 00:00:00

abstract：:Living organisms detect changes in temperature using thermosensory molecules. However, these molecules and/or their mechanisms for sensing temperature differ among organisms. To identify thermosensory molecules in plants, we investigated chloroplast positioning in response to temperature changes and identified a blue-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujii Y,Tanaka H,Konno N,Ogasawara Y,Hamashima N,Tamura S,Hasegawa S,Hayasaki Y,Okajima K,Kodama Y

更新日期：2017-08-22 00:00:00

abstract：:We demonstrate the use of self-assembly for the integration of freestanding micrometer-scale components, including single-crystal, silicon field-effect transistors (FETs) and diffusion resistors, onto flexible plastic substrates. Preferential self-assembly of multiple microcomponent types onto a common platform is ach...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stauth SA,Parviz BA

更新日期：2006-09-19 00:00:00

abstract：:Synthesis of mouse metallothionein (MT)-I and MT-II is transcriptionally induced by the synthetic glucocorticoid, dexamethasone (DEX) or both in vivo as well as in numerous cell lines. However, the location(s) of a glucocorticoid response element (GRE) has not been described. The observation that a marked MT-I gene, a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kelly EJ,Sandgren EP,Brinster RL,Palmiter RD

更新日期：1997-09-16 00:00:00

abstract：:The synthesis of the proteins laminin and collagen IV is stimulated approximately equal to 20-fold in F9 mouse teratocarcinoma stem cells after treatment of the cells with retinoic acid and N6, O2'-dibutyryl-cAMP (Bt2cAMP). A cDNA library from F9 cells treated with retinoic acid, Bt2cAMP, and theophylline (F9-R + DBC ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang SY,Gudas LJ

更新日期：1983-10-01 00:00:00

abstract：:The in vitro biological activities of several rabies virus-neutralizing monoclonal antibodies (mAbs) were compared with their ability to prevent a lethal rabies virus encephalomyelitis. The protective activity of a particular mAb in vivo did not correlate with its virus-neutralizing activity in vitro; rather it was re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dietzschold B,Kao M,Zheng YM,Chen ZY,Maul G,Fu ZF,Rupprecht CE,Koprowski H

更新日期：1992-08-01 00:00:00

abstract：:The default mode network (DMN) has been suggested to support a variety of self-referential functions in humans and has been fractionated into subsystems based on distinct responses to cognitive tasks and functional connectivity architecture. Such subsystems are thought to reflect functional hierarchy and segregation w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu LM,Liang X,Gu H,Brynildsen JK,Stark JA,Ash JA,Lin CP,Lu H,Rapp PR,Stein EA,Yang Y

更新日期：2016-08-02 00:00:00

abstract：:CTCF sites are abundant in the genomes of diverse species but their function is enigmatic. We used chromosome conformation capture to determine long-range interactions among CTCF/cohesin sites over 2 Mb on human chromosome 11 encompassing the beta-globin locus and flanking olfactory receptor genes. Although CTCF occup...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hou C,Dale R,Dean A

更新日期：2010-02-23 00:00:00

abstract：:A major goal for developmental biologists is to define the behaviors and molecular contents of differentiating cells. We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown JL,Snir M,Noushmehr H,Kirby M,Hong SK,Elkahloun AG,Feldman B

更新日期：2008-08-26 00:00:00

abstract：:Deamidation of asparaginyl and glutaminyl residues causes time-dependent changes in charge and conformation of peptides and proteins. Quantitative and experimentally verified predictive calculations of the deamidation rates of 1,371 asparaginyl residues in a representative collection of 126 human proteins have been pe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson NE,Robinson AB

更新日期：2001-10-23 00:00:00

abstract：:Hatchlings of the painted turtle (Chrysemys picta marginata) are unique as the only reptile and highest vertebrate life form known to tolerate the natural freezing of extracellular body fluids during winter hibernation. Turtles survived frequent exposures to temperatures as low as -6 degrees C to -8 degrees C in their...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Storey KB,Storey JM,Brooks SP,Churchill TA,Brooks RJ

更新日期：1988-11-01 00:00:00

abstract：:Unprecedented population declines and extinctions because of human activities, combined with a growing recognition that such losses affect the stability of ecosystems, underscore the need to better understand how populations persist naturally. We provide field experimental evidence that high biodiversity-in particular...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carr MH,Anderson TW,Hixon MA

更新日期：2002-08-20 00:00:00

abstract：:FIP-3 (14.7K interacting protein) was discovered during a search for cell proteins that could interact with an adenovirus protein (Ad E3-14.7K) that had been shown to prevent tumor necrosis factor (TNF)-alpha-induced cytolysis. FIP-3, which contains leucine zippers and a zinc finger domain, inhibits both basal and ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Y,Kang J,Friedman J,Tarassishin L,Ye J,Kovalenko A,Wallach D,Horwitz MS

更新日期：1999-02-02 00:00:00

abstract：:In diabetes, the death of insulin-producing beta-cells by apoptosis leads to insulin deficiency. The lower prevalence of diabetes in females suggests that female sex steroids protect from beta-cell injury. Consistent with this hypothesis, 17beta-estradiol (estradiol) manifests antidiabetic actions in humans and rodent...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le May C,Chu K,Hu M,Ortega CS,Simpson ER,Korach KS,Tsai MJ,Mauvais-Jarvis F

更新日期：2006-06-13 00:00:00

abstract：:An unusual cAMP signaling system mediates many of the events that prepare spermatozoa to meet the egg. Its components include the atypical, bicarbonate-stimulated, sperm adenylyl cyclase and a cAMP-dependent protein kinase (PKA) with the unique catalytic subunit termed Calpha(2) or C(s). We generated mice that lack Ca...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nolan MA,Babcock DF,Wennemuth G,Brown W,Burton KA,McKnight GS

更新日期：2004-09-14 00:00:00

abstract：:Incubation of sickle cells with cystamine, a thiol reagnet, resulted in the formation of an intracellular S-ethylamine derivative. The rate of the reaction was dependent upon the cystamine concentration, the temperature, and the duration of the incubation. The cystamine-treated cells demonstrated a marked inhibition o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hassan W,Beuzard Y,Rosa J

更新日期：1976-09-01 00:00:00

abstract：:Buffering variability in morphogen distribution is essential for reproducible patterning. A theoretically proposed class of mechanisms, termed "distal pinning," achieves robustness by combining local sensing of morphogen levels with global modulation of gradient spread. Here, we demonstrate a critical role for morphog...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rahimi N,Carmon S,Averbukh I,Khajouei F,Sinha S,Schejter ED,Barkai N,Shilo BZ

更新日期：2020-01-21 00:00:00

abstract：:We have determined the nucleotide sequence of the dihydrofolate reductase-thymidylate synthetase (DHFR-TS) gene of the protozoan parasite Leishmania major (dihydrofolate reductase, EC 1.5.1.3 and thymidylate synthase, EC 2.1.1.45). The DHFR-TS protein is encoded by a single 1560-base-pair open reading frame within gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beverley SM,Ellenberger TE,Cordingley JS

更新日期：1986-04-01 00:00:00

abstract：:Metazoan arrestins bind to seven-transmembrane (7TM) receptors to regulate function. Aspergillus nidulans PalF, a protein involved in the fungal ambient pH signaling pathway, contains arrestin N-terminal and C-terminal domains and binds strongly to two different regions within the C-terminal cytoplasmic tail of the 7T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Herranz S,Rodríguez JM,Bussink HJ,Sánchez-Ferrero JC,Arst HN Jr,Peñalva MA,Vincent O

更新日期：2005-08-23 00:00:00

abstract：:A study has been made of Cauchy's problem for a class of abstract parabolic differential equations. Our study is based upon a transformation that maps solutions of second-order abstract Cauchy problems into solutions of first-order. This is a preliminary report on some of the results obtained. These results include (1...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donaldson JA

更新日期：1971-09-01 00:00:00

abstract：:The putative oxidation of hydroethidine (HE) has become a widely used fluorescent assay for the detection of superoxide in cultured cells. By covalently joining HE to a hexyl triphenylphosphonium cation (Mito-HE), the HE moiety can be targeted to mitochondria. However, the specificity of HE and Mito-HE for superoxide ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson KM,Janes MS,Pehar M,Monette JS,Ross MF,Hagen TM,Murphy MP,Beckman JS

更新日期：2006-10-10 00:00:00

abstract：:Cubic cage compounds composed of Co-CN-Ru linkages have been prepared which illustrate the following features: (i) new motifs for alkali metal ion complexation (i.e., cationic receptors for cations), (ii) a new family of triaza-metalloligands, and (iii) a double box-like cage. The cages are synthesized by the condensa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Contakes SM,Kuhlman ML,Ramesh M,Wilson SR,Rauchfuss TB

更新日期：2002-04-16 00:00:00