In vivo protein-DNA interactions at human DNA replication origin.

abstract：:Staphylococcus aureus surface protein SasG promotes cell-cell adhesion during the accumulation phase of biofilm formation, but the molecular basis of this interaction remains poorly understood. Here, we unravel the mechanical properties of SasG on the surface of living bacteria, that is, in its native cellular environ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Formosa-Dague C,Speziale P,Foster TJ,Geoghegan JA,Dufrêne YF

更新日期：2016-01-12 00:00:00

abstract：:The PsaF-deficient mutant 3bF of Chlamydomonas reinhardtii was used to modify PsaF by nuclear transformation and site-directed mutagenesis. Four lysine residues in the N-terminal domain of PsaF, which have been postulated to form the positively charged face of a putative amphipathic alpha-helical structure were altere...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hippler M,Drepper F,Haehnel W,Rochaix JD

更新日期：1998-06-23 00:00:00

abstract：:DNA replication is a core biological process that occurs in prokaryotic cells at high speeds (∼1 nucleotide residue added per millisecond) and with high fidelity (fewer than one misincorporation event per 107 nucleotide additions). The ssDNA binding protein [gene product 32 (gp32)] of the T4 bacteriophage is a central...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phelps C,Israels B,Jose D,Marsh MC,von Hippel PH,Marcus AH

更新日期：2017-05-02 00:00:00

abstract：:Autonomously replicating sequence (ARS) elements of the fission yeast Schizosaccharomyces pombe contain multiple imperfect copies of the consensus sequence reported by Maundrell et al. [Maundrell K., Hutchison, A. & Shall, S. (1988) EMBO J. 7, 2203-2209]. When cell free extracts of S. pombe were incubated with a dimer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murakami Y,Huberman JA,Hurwitz J

更新日期：1996-01-09 00:00:00

abstract：:Mutations in the DAX-1 [dosage-sensitive sex reversal-adrenal hypoplasia congenita (AHC) critical region on the X chromosome; NR0B1] gene cause X-linked AHC associated with hypogonadotropic hypogonadism. DAX-1 encodes an unusual orphan member of the nuclear hormone receptor superfamily, acting as a transcriptional rep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lehmann SG,Lalli E,Sassone-Corsi P

更新日期：2002-06-11 00:00:00

abstract：:The sigma-N (sigmaN) subunit of the bacterial RNA polymerase is a sequence specific DNA-binding protein. The RNA polymerase holoenzyme formed with sigmaN binds to promoters in an inactive form and only initiates transcription when activated by enhancer-binding positive control proteins. We now provide evidence to show...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cannon WV,Chaney MK,Wang X,Buck M

更新日期：1997-05-13 00:00:00

abstract：:The Zika virus (ZIKV) causes microcephaly and the Guillain-Barré syndrome. Little is known about how ZIKV causes these conditions or which ZIKV viral protein(s) is responsible for the associated ZIKV-induced cytopathic effects, including cell hypertrophy, growth restriction, cell-cycle dysregulation, and cell death. W...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li G,Poulsen M,Fenyvuesvolgyi C,Yashiroda Y,Yoshida M,Simard JM,Gallo RC,Zhao RY

更新日期：2017-01-17 00:00:00

abstract：:The role played by oligodendrocytes (OLs), the myelinating cells of the CNS, during brain development has not been fully explored. We have addressed this question by inducing a temporal and reversible ablation of OLs on postnatal CNS development. OL ablation in newborn mice leads to a profound alteration in the struct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Collin L,Usiello A,Erbs E,Mathis C,Borrelli E

更新日期：2004-01-06 00:00:00

abstract：:Focal electric stimulation of the auditory cortex is well suited for exploration of the function of the corticofugal (descending) system and the neural mechanism of plasticity in the central auditory system, because it evokes changes in frequency-tuning, called best frequency (BF) shifts, as does auditory fear conditi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao Z,Suga N

更新日期：2005-12-27 00:00:00

abstract：:Epidermal growth factor (EGF) stimulates membrane protein phosphorylation in a human cell line, A-431. The known hepatic mitogenic action of EGF and the reduction in EGF receptor number that occurs during liver regeneration led us to study whether EGF-dependent protein kinase activity was present in rat liver and whet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rubin RA,O'Keefe EJ,Earp HS

更新日期：1982-02-01 00:00:00

abstract：:The effects of predator intimidation on habitat use and behavior of prey species are rarely quantified for large marine vertebrates over ecologically relevant scales. Using state space movement models followed by a series of step selection functions, we analyzed movement data of concurrently tracked prey, bowhead whal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matthews CJD,Breed GA,LeBlanc B,Ferguson SH

更新日期：2020-03-24 00:00:00

abstract：:A somatic mutation in the X linked PIGA gene is responsible for the deficiency of glycosyl phosphatidylinositol (GPI)-anchored proteins on blood cells from patients with paroxysmal nocturnal hemoglobinuria. No inherited form of GPI-anchor deficiency has been described. Because conventional Piga gene knockout is associ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keller P,Tremml G,Rosti V,Bessler M

更新日期：1999-06-22 00:00:00

abstract：:Navigation, the ability to reach desired goal locations, is critical for animals and humans. Animal navigation has been studied extensively in birds, insects, and some marine vertebrates and invertebrates, yet we are still far from elucidating the underlying mechanisms in other taxonomic groups, especially mammals. He...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsoar A,Nathan R,Bartan Y,Vyssotski A,Dell'Omo G,Ulanovsky N

更新日期：2011-09-13 00:00:00

abstract：:We measured the superconducting transition temperature of (6)Li between 16 and 26 GPa, and report the lightest system to exhibit superconductivity to date. The superconducting phase diagram of (6)Li is compared with that of (7)Li through simultaneous measurement in a diamond anvil cell (DAC). Below 21 GPa, Li exhibits...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaeffer AM,Temple SR,Bishop JK,Deemyad S

更新日期：2015-01-06 00:00:00

abstract：:Volume changes associated with protein folding reactions contain valuable information about the folding mechanism and the nature of the transition state. However, meaningful interpretation of such data requires that overall volume changes be deconvoluted into individual contributions from different structural componen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neumaier S,Büttner M,Bachmann A,Kiefhaber T

更新日期：2013-12-24 00:00:00

abstract：:To study the mechanism of arrest of DNA synthesis at d(TC)n and d(GA)n sequences, single-stranded DNA molecules including d(TC)27 or d(TC)31 tracts or a d(GA)27 tract were used as templates for in vitro assays of complementary DNA synthesis performed by extension of a primer with the Klenow polymerase or the Taq polym...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baran N,Lapidot A,Manor H

更新日期：1991-01-15 00:00:00

abstract：:Although sexual selection theory has proved successful in explaining a wide array of male ornaments, the function of ornaments occurring in females is largely unknown. Traditionally, female ornaments have been considered nonfunctional, being merely a genetically correlated response to selection for male ornamentation....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Amundsen T,Forsgren E

更新日期：2001-11-06 00:00:00

abstract：:Human peripheral blood lymphocytes (PBLs) were transduced with a number of recombinant retroviruses including RRz2, an LNL6-based virus with a ribozyme targeted to the human immunodeficiency virus (HIV) tat gene transcript inserted within the 3' region of the neomycin-resistance gene; RASH5, and LNHL-based virus conta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun LQ,Pyati J,Smythe J,Wang L,Macpherson J,Gerlach W,Symonds G

更新日期：1995-08-01 00:00:00

abstract：:Proteins can bind target molecules through either induced fit or conformational selection pathways. In the conformational selection model, a protein samples a scarcely populated high-energy state that resembles a target-bound conformation. In enzymatic catalysis, such high-energy states have been identified as crucial...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kovermann M,Grundström C,Sauer-Eriksson AE,Sauer UH,Wolf-Watz M

更新日期：2017-06-13 00:00:00

abstract：:In 1989, populations of North American gypsy moth, Lymantria dispar, in seven contiguous northeastern states were severely reduced by a fungal pathogen. Based on morphology, development, and pathology, this organism appeared to be Entomophaga maimaiga. We have now used allozyme and restriction fragment length polymorp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hajek AE,Humber RA,Elkinton JS,May B,Walsh SR,Silver JC

更新日期：1990-09-01 00:00:00

abstract：:Life-long protection from disease through immunization can be accomplished through individual or community protection. Individual protection is the goal for vaccination against diseases that have inanimate or animal reservoirs or that pose risks for certain populations. Community protection is the goal for vaccination...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dowdle WR,Orenstein WA

更新日期：1994-03-29 00:00:00

abstract：:Absorbance changes in untreated intact leaf discs, produced upon excitation with high-intensity red light, were shown to be due to the photooxidation of cytochrome b-559. At low intensities (<100 W/m2), photooxidation was almost undetectable. Photooxidation occurred with a half-time of 4.3 sec and an extent of 0.64 mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Canaani O,Havaux M

更新日期：1990-12-01 00:00:00

abstract：:Many native proteins occasionally form partially unfolded forms (PUFs), which can be detected by hydrogen/deuterium exchange and NMR spectroscopy. Knowledge about these metastable states is required to better understand the onset of folding-related diseases. So far, not much is known about where PUFs reside within the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bollen YJ,Kamphuis MB,van Mierlo CP

更新日期：2006-03-14 00:00:00

abstract：:Millions of individuals routinely remain awake for more than 18 h daily, which causes performance decrements. It is unknown if these functional impairments are the result of that extended wakefulness or from the associated shortened sleep durations. We therefore examined changes in objective reaction time performance ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McHill AW,Hull JT,Wang W,Czeisler CA,Klerman EB

更新日期：2018-06-05 00:00:00

abstract：:Dopamine causes a significant retraction of neurites of bull-head catfish horizontal cells maintained in culture. The effects of dopamine are blocked by haloperidol and SCH 23390, a D1 antagonist, but not by sulpiride, a D2 antagonist. The dopamine-induced morphological changes were mimicked by SKF 38393, a D1 agonist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rodrigues Pdos S,Dowling JE

更新日期：1990-12-01 00:00:00

abstract：:We have determined the general constraints that govern sequence divergence in proteins that retain entirely, or very largely, the same structure and function. To do this we collected data from three different groups of orthologous sequences: those found in humans and mice, in humans and chickens, and in Escherichia co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sasidharan R,Chothia C

更新日期：2007-06-12 00:00:00

abstract：:The Fbw7 (F-box/WD repeat-containing protein 7) ubiquitin ligase targets multiple oncoproteins for degradation and is commonly mutated in cancers. Like other pleiotropic tumor suppressors, Fbw7's complex biology has impeded our understanding of how Fbw7 mutations promote tumorigenesis and hindered the development of t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis RJ,Gönen M,Margineantu DH,Handeli S,Swanger J,Hoellerbauer P,Paddison PJ,Gu H,Raftery D,Grim JE,Hockenbery DM,Margolin AA,Clurman BE

更新日期：2018-05-22 00:00:00

abstract：:The Landau spectrum of bismuth is complex and includes many angle-dependent lines in the extreme quantum limit. The adequacy of single-particle theory to describe this spectrum in detail has been an open issue. Here, we present a study of angle-resolved Nernst effect in bismuth, which maps the angle-resolved Landau sp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhu Z,Fauqué B,Malone L,Antunes AB,Fuseya Y,Behnia K

更新日期：2012-09-11 00:00:00

abstract：:Several hypolipidemic drugs and certain industrial plasticizers induce proliferation of peroxisomes, enhance the activity of peroxisome-associated beta-oxidation of fatty acids, and produce hepatocellular carcinomas in the livers of rodents. Because these chemicals themselves are not mutagens and do not covalently mod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fahl WE,Lalwani ND,Watanabe T,Goel SK,Reddy JK

更新日期：1984-12-01 00:00:00

abstract：:Current issues pertaining to theoretical simulations of materials, with a focus on systems of nanometer-scale dimensions, are discussed. The use of atomistic simulations as high-resolution numerical experiments, enabling and guiding formulation and testing of analytic theoretical descriptions, is demonstrated through ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Landman U

更新日期：2005-05-10 00:00:00