Protein phosphatase 2C binds selectively to and dephosphorylates metabotropic glutamate receptor 3.

abstract：:Sleep is essential for basic survival, and insufficient sleep leads to a variety of dysfunctions. In humans, one of the most profound consequences of sleep deprivation is imprecise or irrational communication, demonstrated by degradation in signaling as well as in receiving information. Communication in nonhuman anima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klein BA,Klein A,Wray MK,Mueller UG,Seeley TD

更新日期：2010-12-28 00:00:00

abstract：:Almost all democratic societies evolved socially and politically out of authoritarian and nondemocratic regimes. These changes not only altered the allocation of economic resources in society but also the structure of political power. In this paper, we develop a framework for studying the dynamics of political and soc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acemoglu D,Egorov G,Sonin K

更新日期：2011-12-27 00:00:00

abstract：:Current issues pertaining to theoretical simulations of materials, with a focus on systems of nanometer-scale dimensions, are discussed. The use of atomistic simulations as high-resolution numerical experiments, enabling and guiding formulation and testing of analytic theoretical descriptions, is demonstrated through ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Landman U

更新日期：2005-05-10 00:00:00

abstract：:Clostridium kluyveri grown in the presence of 1 muM Na2(75)SeO3 produces a thiolase that copurifies with 75Se. Based on several criteria, the selenium moiety in this protein is selenomethionine. The 75Se-labeled amino acid in acid hydrolysates of the radioactive protein cochromatographed with authentic selenomethionin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hartmanis MG,Stadtman TC

更新日期：1982-08-01 00:00:00

abstract：:Lateral organ distribution at the shoot apical meristem defines specific and robust phyllotaxis patterns that have intrigued biologists and mathematicians for centuries. In silico studies have revealed that this self-organizing process can be recapitulated by modeling the polar transport of the phytohormone auxin. Phy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pinon V,Prasad K,Grigg SP,Sanchez-Perez GF,Scheres B

更新日期：2013-01-15 00:00:00

abstract：:A technique for systematic peptide variation by a combination of rational and evolutionary approaches is presented. The design scheme consists of five consecutive steps: (i) identification of a "seed peptide" with a desired activity, (ii) generation of variants selected from a physicochemical space around the seed pep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schneider G,Schrödl W,Wallukat G,Müller J,Nissen E,Rönspeck W,Wrede P,Kunze R

更新日期：1998-10-13 00:00:00

abstract：:Molecular junctions are essentially modified electrodes familiar to electrochemists where the electrolyte is replaced by a conducting "contact." It is generally hypothesized that changing molecular structure will alter system energy levels leading to a change in the transport barrier. Here, we show the conductance of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sayed SY,Fereiro JA,Yan H,McCreery RL,Bergren AJ

更新日期：2012-07-17 00:00:00

abstract：:We report on preparation of rhodopsin proteoliposomes with the cytoplasmic domain of rhodopsin facing the exterior of the proteoliposomes. Rhodopsin purified from rod outer segments of bovine retinae by immunoaffinity chromatography in octyl glucoside was reconstituted into liposomes prepared from soybean phospholipid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Niu L,Kim JM,Khorana HG

更新日期：2002-10-15 00:00:00

abstract：:We have constructed three different fusion genes containing the herpes simplex virus thymidine kinase (HSV tk) and the bacterial neomycin phosphotransferase (neo) genes. All three fusion genes utilize the HSV tk promoter but differ at the junction of their components. We have determined if the fusion genes are bifunct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz F,Maeda N,Smithies O,Hickey R,Edelmann W,Skoultchi A,Kucherlapati R

更新日期：1991-12-01 00:00:00

abstract：:The regulatory mechanisms underlying the uptake and utilization of multiple types of carbohydrates in actinomycetes remain poorly understood. In this study, we show that GlnR (central regulator of nitrogen metabolism) serves as a universal regulator of nitrogen metabolism and plays an important, previously unknown rol...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liao CH,Yao L,Xu Y,Liu WB,Zhou Y,Ye BC

更新日期：2015-12-22 00:00:00

abstract：:Protein S (ProS) and growth arrest-specific 6 (Gas6) bind to phosphatidylserine (PtdSer) and induce efferocytosis upon binding TAM-family receptors (Tyro3, Axl, and Mer). Here, we produced mouse ProS, Gas6, and TAM-receptor extracellular region fused to IgG fragment crystallizable region in HEK293T cells. ProS and Gas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yanagihashi Y,Segawa K,Maeda R,Nabeshima YI,Nagata S

更新日期：2017-08-15 00:00:00

abstract：:Alpha-actinin-4 is a widely expressed protein that employs an actin-binding site with two calponin homology domains to crosslink actin filaments (F-actin) in a Ca(2+)-sensitive manner in vitro. An inherited, late-onset form of kidney failure is caused by point mutations in the alpha-actinin-4 actin-binding domain. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weins A,Schlondorff JS,Nakamura F,Denker BM,Hartwig JH,Stossel TP,Pollak MR

更新日期：2007-10-09 00:00:00

abstract：:Developmental constraint is indicated when one finds that similar genetic mechanisms are responsible for independent origins of the same derived phenotype. We studied three independent origins of rosette flowering within the mustard family and attempted to evaluate the extent to which the same mechanisms were involved...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yoon HS,Baum DA

更新日期：2004-04-27 00:00:00

abstract：:Mice lacking the beta1-subunit (gene, Kcnmb1; protein, BK-beta1) of the large Ca-activated K channel (BK) are hypertensive. This phenotype is thought to result from diminished BK currents in vascular smooth muscle where BK-beta1 is an ancillary subunit. However, the beta1-subunit is also expressed in the renal connect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grimm PR,Irsik DL,Settles DC,Holtzclaw JD,Sansom SC

更新日期：2009-07-14 00:00:00

abstract：:Whole-cell DNA preparations isolated from SC-1 cells chronically infected with N- or B-tropic murine leukemia viruses (MuLV) were tested for infectious activity in an Fv-1n (NIH-3T3) and two Fv-Ib (C57BL/6 and SV-A31) cell cultures. Efficiency of transfection for all DNAs was better in the NIH-3T3 cells than in C57BL/...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu IC,Yang WK,Tennant RW,Brown A

更新日期：1978-03-01 00:00:00

abstract：:Clear-cut inherited Mendelian traits, such as familial adenomatous polyposis or hereditary nonpolyposis colorectal cancer, account for <4% of colorectal cancers. Another 20% of all colorectal cancers are thought to occur in individuals with a significant inherited multifactorial susceptibility to colorectal cancer tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fearnhead NS,Wilding JL,Winney B,Tonks S,Bartlett S,Bicknell DC,Tomlinson IP,Mortensen NJ,Bodmer WF

更新日期：2004-11-09 00:00:00

abstract：:The supposition that agricultural intensification results in land sparing for conservation has become central to policy formulations across the tropics. However, underlying assumptions remain uncertain and have been little explored in the context of conservation incentive schemes such as policies for Reducing Emission...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phelps J,Carrasco LR,Webb EL,Koh LP,Pascual U

更新日期：2013-05-07 00:00:00

abstract：:We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang S,Shan X,Patel U,Huang X,Lu J,Li J,Tao N

更新日期：2010-09-14 00:00:00

abstract：:Identification of the consensus sequence for termination of replication (ter) in Escherichia coli and the isolation of the ter-binding protein (TBP) allowed us to test their effects on replication forks initiated at the unique origin of the E. coli chromosome (oriC) in a purified enzyme system. Replication was severel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee EH,Kornberg A,Hidaka M,Kobayashi T,Horiuchi T

更新日期：1989-12-01 00:00:00

abstract：:CD2 (T11, sheep erythrocyte receptor) is a surface antigen of the human T-lymphocyte lineage. cDNA clones encoding CD2 have been isolated by using the purified, denatured CD2 to raise a rat antiserum. Positive clones were recognized in a phage lambda gt11 expression library prepared from the human leukemia T-cell line...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sewell WA,Brown MH,Dunne J,Owen MJ,Crumpton MJ

更新日期：1986-11-01 00:00:00

abstract：:The spectra of the alpha, beta, and gamma forms of zinc monoarsanilazotyrosine-248 carboxypeptidase A are indistinguishable. At pH 8.2 their crystals are yellow, while their solutions are red, lambda(max) 510 nm. Absorption and circular dichroism-pH titrations of the modified zinc and apoenzymes demonstrate that the a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Johansen JT,Vallee BL

更新日期：1973-07-01 00:00:00

abstract：:A pluripotent cell line, C3H10T1/2, is induced to undergo adipogenesis by a mixture of factors that includes a glucocorticoid such as dexamethasone. We found that expression of myostatin (MSTN), a TGF-beta family member extensively studied in muscle, was induced by dexamethasone under those differentiation conditions....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feldman BJ,Streeper RS,Farese RV Jr,Yamamoto KR

更新日期：2006-10-17 00:00:00

abstract：:N-methyl-d-aspartate receptors (NMDARs) mediate critical CNS functions, whereas excessive activity contributes to neuronal damage. At physiological glycine concentrations, NMDAR currents recorded from cultured rodent hippocampal neurons exhibited strong desensitization in the continued presence of NMDA, thus protectin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： You H,Tsutsui S,Hameed S,Kannanayakal TJ,Chen L,Xia P,Engbers JD,Lipton SA,Stys PK,Zamponi GW

更新日期：2012-01-31 00:00:00

abstract：:The heterodimeric antimicrobial peptide distinctin is composed of 2 linear peptide chains of 22- and 25-aa residues that are connected by a single intermolecular S-S bond. This heterodimer has been considered to be a unique example of a previously unrecorded class of bioactive peptides. Here the 2 distinctin chains we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Resende JM,Moraes CM,Munhoz VH,Aisenbrey C,Verly RM,Bertani P,Cesar A,Piló-Veloso D,Bechinger B

更新日期：2009-09-29 00:00:00

abstract：:Down syndrome (DS) is the most common form of intellectual disability. The cognitive alterations in DS are thought to depend on brain regions critical for learning and memory such as the prefrontal cortex (PFC) and the hippocampus (HPC). Neuroimaging studies suggest that increased brain connectivity correlates with lo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alemany-González M,Gener T,Nebot P,Vilademunt M,Dierssen M,Puig MV

更新日期：2020-05-26 00:00:00

abstract：:Many pathological conditions of the central nervous system involve damage to and removal of myelin membrane. Very little is known about initiation of this membrane damage and the mechanisms of disposal of the damaged tissue. We are interested in the interaction between complement (the components of complement are desi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vanguri P,Koski CL,Silverman B,Shin ML

更新日期：1982-05-01 00:00:00

abstract：:Alzheimer's disease is characterized by two primary pathological features: amyloid plaques and neurofibrillary tangles. The interconnection between amyloid and tau aggregates is of intense interest, but mouse models have yet to reveal a direct interrelationship. We now show that NO may be a key factor that connects am...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colton CA,Vitek MP,Wink DA,Xu Q,Cantillana V,Previti ML,Van Nostrand WE,Weinberg JB,Dawson H

更新日期：2006-08-22 00:00:00

abstract：:We have measured the fidelity of bidirectional, semiconservative DNA synthesis by a human DNA replication complex in vitro. Replication was performed by extracts of HeLa cells in the presence of simian virus 40 (SV40) large tumor antigen by using a double-stranded phage M13mp2 DNA template containing the SV40 origin o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roberts JD,Kunkel TA

更新日期：1988-10-01 00:00:00

abstract：:A method for assessing genomic similarity based on relative abundances of short oligonucleotides in large DNA samples is introduced. The method requires neither homologous sequences nor prior sequence alignments. The analysis centers on (i) dinucleotide (and tri- and tetra-) relative abundance extremes in genomic sequ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Karlin S,Ladunga I

更新日期：1994-12-20 00:00:00

abstract：:The translocation of lipids from the inner to the outer membrane of Escherichia coli has been investigated by pulse-chase experiments. After a pulse with [2-3H]glycerol, the specific activity of the newly synthesized [3H]phosphatidylethanolamine was 5 times greater in the inner than in the outer membrane. During the c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Donohue-Rolfe AM,Schaechter M

更新日期：1980-04-01 00:00:00