Abstract:
:We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect the binding of the viral particles to surfaces functionalized with and without antibodies. We show that the intensity of the particle image is related to the mass of the particle, from which we determine the mass and mass distribution of influenza viral particles with a mass detection limit of approximately 1 ag (or 0.2 fg/mm(2)). This work demonstrates a multiplexed method to measure the masses of individual viral particles and to study the binding activity of the viral particles.
journal_name
Proc Natl Acad Sci U S Aauthors
Wang S,Shan X,Patel U,Huang X,Lu J,Li J,Tao Ndoi
10.1073/pnas.1005264107subject
Has Abstractpub_date
2010-09-14 00:00:00pages
16028-32issue
37eissn
0027-8424issn
1091-6490pii
1005264107journal_volume
107pub_type
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