当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Simultaneous, accurate measurement of the 3D position and orientation of single molecules.

Simultaneous, accurate measurement of the 3D position and orientation of single molecules.

Abstract：

:Recently, single molecule-based superresolution fluorescence microscopy has surpassed the diffraction limit to improve resolution to the order of 20 nm or better. These methods typically use image fitting that assumes an isotropic emission pattern from the single emitters as well as control of the emitter concentration. However, anisotropic single-molecule emission patterns arise from the transition dipole when it is rotationally immobile, depending highly on the molecule's 3D orientation and z position. Failure to account for this fact can lead to significant lateral (x, y) mislocalizations (up to ∼50-200 nm). This systematic error can cause distortions in the reconstructed images, which can translate into degraded resolution. Using parameters uniquely inherent in the double-lobed nature of the Double-Helix Point Spread Function, we account for such mislocalizations and simultaneously measure 3D molecular orientation and 3D position. Mislocalizations during an axial scan of a single molecule manifest themselves as an apparent lateral shift in its position, which causes the standard deviation (SD) of its lateral position to appear larger than the SD expected from photon shot noise. By correcting each localization based on an estimated orientation, we are able to improve SDs in lateral localization from ∼2× worse than photon-limited precision (48 vs. 25 nm) to within 5 nm of photon-limited precision. Furthermore, by averaging many estimations of orientation over different depths, we are able to improve from a lateral SD of 116 (∼4× worse than the photon-limited precision; 28 nm) to 34 nm (within 6 nm of the photon limit).

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Backlund MP,Lew MD,Backer AS,Sahl SJ,Grover G,Agrawal A,Piestun R,Moerner WE

doi

10.1073/pnas.1216687109

subject

Has Abstract

pub_date

2012-11-20 00:00:00

pages

19087-92

issue

47

eissn

0027-8424

issn

1091-6490

pii

1216687109

journal_volume

109

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Chloroplast SRP43 acts as a chaperone for glutamyl-tRNA reductase, the rate-limiting enzyme in tetrapyrrole biosynthesis.

    abstract：:Assembly of light-harvesting complexes requires synchronization of chlorophyll (Chl) biosynthesis with biogenesis of light-harvesting Chl a/b-binding proteins (LHCPs). The chloroplast signal recognition particle (cpSRP) pathway is responsible for transport of nucleus-encoded LHCPs in the stroma of the plastid and thei...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1719645115

    authors： Wang P,Liang FC,Wittmann D,Siegel A,Shan SO,Grimm B

    更新日期：2018-04-10 00:00:00

  • Probing the evolution of appendage specialization by Hox gene misexpression in an emerging model crustacean.

    abstract：:Changes in the expression of Hox genes have been widely linked to the evolution of animal body plans, but functional demonstrations of this relationship have been impeded by the lack of suitable model organisms. A classic case study involves the repeated evolution of specialized feeding appendages, called maxillipeds,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0902804106

    authors： Pavlopoulos A,Kontarakis Z,Liubicich DM,Serano JM,Akam M,Patel NH,Averof M

    更新日期：2009-08-18 00:00:00

  • Model for the role of macromolecular crowding in regulation of cellular volume.

    abstract：:A simple model is proposed to account for large increases in transporter-mediated ion flux across cell membranes that are elicited by small fractional changes of cell volume. The model is based upon the concept that, as a result of large excluded volume effects in cytoplasm (macromolecular crowding), the tendency of s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.21.10504

    authors： Minton AP,Colclasure GC,Parker JC

    更新日期：1992-11-01 00:00:00

  • Molecular characterization of two mammalian bHLH-PAS domain proteins selectively expressed in the central nervous system.

    abstract：:Here we describe two mammalian transcription factors selectively expressed in the central nervous system. Both proteins, neuronal PAS domain protein (NPAS) 1 and NPAS2, are members of the basic helix-loop-helix-PAS family of transcription factors. cDNAs encoding mouse and human forms of NPAS1 and NPAS2 have been isola...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.2.713

    authors： Zhou YD,Barnard M,Tian H,Li X,Ring HZ,Francke U,Shelton J,Richardson J,Russell DW,McKnight SL

    更新日期：1997-01-21 00:00:00

  • A TNFSF15 disease-risk polymorphism increases pattern-recognition receptor-induced signaling through caspase-8-induced IL-1.

    abstract：:Inflammatory diseases are characterized by dysregulated cytokine production. Altered functions for most risk loci, including the inflammatory bowel disease and leprosy-associated tumor necrosis factor ligand superfamily member 15 (TNFSF15) region, are unclear. Regulation of pattern-recognition-receptor (PRR)-induced s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1404178111

    authors： Hedl M,Abraham C

    更新日期：2014-09-16 00:00:00

  • Electroconvulsive seizure and VEGF increase the proliferation of neural stem-like cells in rat hippocampus.

    abstract：:All classes of antidepressants increase hippocampal cell proliferation and neurogenesis, which contributes, in part, to the behavioral actions of these treatments. Among antidepressant treatments, electroconvulsive seizure (ECS) is the most robust stimulator of hippocampal cell proliferation and the most efficacious t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0710858105

    authors： Segi-Nishida E,Warner-Schmidt JL,Duman RS

    更新日期：2008-08-12 00:00:00

  • Disparate foundations of scientists' policy positions on contentious biomedical research.

    abstract：:What drives scientists' position taking on matters where empirical answers are unavailable or contradictory? We examined the contentious debate on whether to limit experiments involving the creation of potentially pandemic pathogens. Hundreds of scientists, including Nobel laureates, have signed petitions on the debat...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1613580114

    authors： Edelmann A,Moody J,Light R

    更新日期：2017-06-13 00:00:00

  • Phosphoinositol lipids bind to phosphatidylinositol 3 (PI3)-kinase enhancer GTPase and mediate its stimulatory effect on PI3-kinase and Akt signalings.

    abstract：:Phosphatidylinositol 3 (PI3)-kinase enhancer (PIKE) is a nuclear GTPase that enhances PI3-kinase activity in a GTP-dependent manner. Both PIKE-L and -A isoforms contain GTPase, pleckstrin homology (PH), ADP ribosylation factor-GTPase-activating protein, and two ankyrin repeats domains, and C-terminal ADP ribosylation ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0507365102

    authors： Hu Y,Liu Z,Ye K

    更新日期：2005-11-15 00:00:00

  • Human leukemia-associated anti-nuclear reactivity.

    abstract：:A brilliant, coarsely granular nuclear antigen was detected by anti-complement immunofluorescence in the nuclei of acute myeloid leukemia myeloblasts. Designated as LANA (leukemia-associated nuclear antigen), the reactivity differs from that of the Epstein-Barr-virus-determined nuclear antigen (EBNA) in immunological ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.71.3.685

    authors： Klein G,Steiner M,Wiener F,Klein E

    更新日期：1974-03-01 00:00:00

  • Intrathymic maturation of murine T lymphocytes from CD8+ precursors.

    abstract：:The CD4-8- thymocyte subset contains immature precursors for phenotypically and functionally mature CD4+8- and CD4-8+ thymocytes and peripheral T cells, as well as nonmature CD4+8+ thymocytes, most of which die in situ. The intrathymic death of most thymocytes is probably related to selective influences that ensure th...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.19.7542

    authors： Guidos CJ,Weissman IL,Adkins B

    更新日期：1989-10-01 00:00:00

  • Nephrin is specifically located at the slit diaphragm of glomerular podocytes.

    abstract：:We describe here the size and location of nephrin, the first protein to be identified at the glomerular podocyte slit diaphragm. In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.14.7962

    authors： Ruotsalainen V,Ljungberg P,Wartiovaara J,Lenkkeri U,Kestilä M,Jalanko H,Holmberg C,Tryggvason K

    更新日期：1999-07-06 00:00:00

  • Vibrio cholerae type 6 secretion system effector trafficking in target bacterial cells.

    abstract：:The type 6 secretion system (T6SS) is used by many Gram-negative bacterial species to deliver toxic effector proteins into nearby bacteria prey cells to kill or inhibit their growth. VgrG proteins are core conserved secretion substrates of the T6SS and one subset of T6SS effectors consists of VgrG proteins with C-term...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1711219114

    authors： Ho BT,Fu Y,Dong TG,Mekalanos JJ

    更新日期：2017-08-29 00:00:00

  • Genes for synapsin I, a neuronal phosphoprotein, map to conserved regions of human and murine X chromosomes.

    abstract：:Synapsin I is a neuron-specific phosphoprotein associated with the membranes of small synaptic vesicles. Its function is not entirely clear, but evidence points to a possible role in the regulation of neurotransmitter release. Its biosynthesis is under developmental control. Assignment of the human synapsin I gene to ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.22.8679

    authors： Yang-Feng TL,DeGennaro LJ,Francke U

    更新日期：1986-11-01 00:00:00

  • Sir-two-homolog 2 (Sirt2) modulates peripheral myelination through polarity protein Par-3/atypical protein kinase C (aPKC) signaling.

    abstract：:The formation of myelin by Schwann cells (SCs) occurs via a series of orchestrated molecular events. We previously used global expression profiling to examine peripheral nerve myelination and identified the NAD(+)-dependent deacetylase Sir-two-homolog 2 (Sirt2) as a protein likely to be involved in myelination. Here, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1104969108

    authors： Beirowski B,Gustin J,Armour SM,Yamamoto H,Viader A,North BJ,Michán S,Baloh RH,Golden JP,Schmidt RE,Sinclair DA,Auwerx J,Milbrandt J

    更新日期：2011-10-25 00:00:00

  • ATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing.

    abstract：:Matching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP:NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin-Benson-Bassham (CBB) cycle. Several different mechanisms to increase ATP availabi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1711497115

    authors： Voon CP,Guan X,Sun Y,Sahu A,Chan MN,Gardeström P,Wagner S,Fuchs P,Nietzel T,Versaw WK,Schwarzländer M,Lim BL

    更新日期：2018-11-06 00:00:00

  • Dermorphin-related peptides from the skin of Phyllomedusa bicolor and their amidated analogs activate two mu opioid receptor subtypes that modulate antinociception and catalepsy in the rat.

    abstract：:Three naturally occurring dermorphin-like peptides from the skin of the frog Phyllomedusa bicolor, the related carboxyl-terminal amides, and some substituted analogs were synthesized, their binding profiles to opioid receptors were determined, and their biological activities were studied in isolated organ preparations...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.15.7203

    authors： Negri L,Erspamer GF,Severini C,Potenza RL,Melchiorri P,Erspamer V

    更新日期：1992-08-01 00:00:00

  • Dominant-negative action of the jimpy mutation in mice complemented with an autosomal transgene for myelin proteolipid protein.

    abstract：:Mutations in genes encoding membrane proteins have been associated with cell death of unknown cause from invertebrate development to human degenerative diseases. A point mutation in the gene for myelin proteolipid protein (PLP) underlies oligodendrocyte death and dysmyelination in jimpy mice, an accurate model for Pel...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.10.4447

    authors： Schneider AM,Griffiths IR,Readhead C,Nave KA

    更新日期：1995-05-09 00:00:00

  • Phylogenetic analyses reveal the shady history of C4 grasses.

    abstract：:Grasslands cover more than 20% of the Earth's terrestrial surface, and their rise to dominance is one of the most dramatic events of biome evolution in Earth history. Grasses possess two main photosynthetic pathways: the C(3) pathway that is typical of most plants and a specialized C(4) pathway that minimizes photores...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0909672107

    authors： Edwards EJ,Smith SA

    更新日期：2010-02-09 00:00:00

  • Two insulin-like growth factor I messenger RNAs are expressed in human liver.

    abstract：:Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precur...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.1.77

    authors： Rotwein P

    更新日期：1986-01-01 00:00:00

  • A proposed common allosteric mechanism for active transport, muscle contraction, and ribosomal translocation.

    abstract：:It is suggested that active transport, muscle contraction, and ribosomal translocation may all make use of a common allosteric mechanism in which ATP or GTP serves as both the effector and substrate and in which a conformational change in a protein (enzyme) moves or exerts a force on a second ligand. The enzymatic spl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.64.1.267

    authors： Hill TL

    更新日期：1969-09-01 00:00:00

  • Modulation of epidermal growth factor receptors by human alpha interferon.

    abstract：:Treatment of Madin-Darby bovine kidney (MDBK) cells with human interferon (IFN)-alpha 2 at 37 degrees C results in a dose-dependent inhibition of cell growth and a reduction of the subsequent binding of 125I-labeled epidermal growth factor (EGF) at 4 degrees C. Human IFN-beta and -gamma, which exhibit little antiviral...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.83.21.8226

    authors： Zoon KC,Karasaki Y,zur Nedden DL,Hu RQ,Arnheiter H

    更新日期：1986-11-01 00:00:00

  • Legionella pneumophila mip gene potentiates intracellular infection of protozoa and human macrophages.

    abstract：:Legionella pneumophila is an intracellular parasite of freshwater protozoa and human macrophages. Recent studies determined that the macrophage infectivity potentiator (Mip) surface protein, a prokaryotic homolog of the FK506-binding proteins, is required for optimal infection of macrophages. To determine whether Mip ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.11.5188

    authors： Cianciotto NP,Fields BS

    更新日期：1992-06-01 00:00:00

  • Junctional adhesion molecule-A-deficient polymorphonuclear cells show reduced diapedesis in peritonitis and heart ischemia-reperfusion injury.

    abstract：:Junctional Adhesion Molecule-A (JAM-A) is a transmembrane adhesive protein expressed at endothelial junctions and in leukocytes. Here we report that JAM-A is required for the correct infiltration of polymorphonuclear leukocytes (PMN) into an inflamed peritoneum or in the heart upon ischemia-reperfusion injury. The def...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0500147102

    authors： Corada M,Chimenti S,Cera MR,Vinci M,Salio M,Fiordaliso F,De Angelis N,Villa A,Bossi M,Staszewsky LI,Vecchi A,Parazzoli D,Motoike T,Latini R,Dejana E

    更新日期：2005-07-26 00:00:00

  • RANK is the intrinsic hematopoietic cell surface receptor that controls osteoclastogenesis and regulation of bone mass and calcium metabolism.

    abstract：:We have generated RANK (receptor activator of NF-kappaB) nullizygous mice to determine the molecular genetic interactions between osteoprotegerin, osteoprotegerin ligand, and RANK during bone resorption and remodeling processes. RANK(-/-) mice lack osteoclasts and have a profound defect in bone resorption and remodeli...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.4.1566

    authors： Li J,Sarosi I,Yan XQ,Morony S,Capparelli C,Tan HL,McCabe S,Elliott R,Scully S,Van G,Kaufman S,Juan SC,Sun Y,Tarpley J,Martin L,Christensen K,McCabe J,Kostenuik P,Hsu H,Fletcher F,Dunstan CR,Lacey DL,Boyle WJ

    更新日期：2000-02-15 00:00:00

  • Aberrantly elevated microRNA-34a in obesity attenuates hepatic responses to FGF19 by targeting a membrane coreceptor β-Klotho.

    abstract：:MicroRNA-34a (miR-34a) is the most highly elevated hepatic miR in obese mice and is also substantially elevated in patients who have steatosis, but its role in obesity and metabolic dysfunction remains unclear. After a meal, FGF19 is secreted from the ileum; binds to a hepatic membrane receptor complex, FGF19 receptor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1205951109

    authors： Fu T,Choi SE,Kim DH,Seok S,Suino-Powell KM,Xu HE,Kemper JK

    更新日期：2012-10-02 00:00:00

  • Top performers are not the most impressive when extreme performance indicates unreliability.

    abstract：:The relationship between performance and ability is a central concern in the social sciences: Are the most successful much more able than others, and are failures unskilled? Prior research has shown that noise and self-reinforcing dynamics make performance unpredictable and lead to a weak association between ability a...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1116048109

    authors： Denrell J,Liu C

    更新日期：2012-06-12 00:00:00

  • Hydrodynamic narrowing of tubes extruded from cells.

    abstract：:We discuss the pulling force f required to extrude a lipid tube from a living cell as a function of the extrusion velocity L. The main feature is membrane friction on the cytoskeleton. As recently observed for neutrophils, the tether force exhibits a "shear thinning" response over a large range of pulling velocities, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0602012103

    authors： Brochard-Wyart F,Borghi N,Cuvelier D,Nassoy P

    更新日期：2006-05-16 00:00:00

  • [4Fe-4S]-cluster-depleted Azotobacter vinelandii ferredoxin I: a new 3Fe iron-sulfur protein.

    abstract：:Fe(CN)6(-3) oxidation of the aerobically isolated 7Fe Azotobacter vinelandii ferredoxin I, (7Fe)FdI, is a degradative reaction. Destruction of the [4Fe-4S] cluster occurs first, followed by destruction of the [3Fe-3S] cluster. At a Fe(CN)6(-3)/(7Fe)FdI concentration ratio of 20, the product is a mixture of apoprotein ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.17.5661

    authors： Stephens PJ,Morgan TV,Devlin F,Penner-Hahn JE,Hodgson KO,Scott RA,Stout CD,Burgess BK

    更新日期：1985-09-01 00:00:00

  • Mathematical model of renal interstitial fibrosis.

    abstract：:Lupus nephritis (LN) is an autoimmune disease that occurs when autoantibodies complex with self-antigen and form immune complexes that accumulate in the glomeruli. These immune complexes initiate an inflammatory response resulting in glomerular injury. LN often concomitantly affects the tubulointerstitial compartment ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1413970111

    authors： Hao W,Rovin BH,Friedman A

    更新日期：2014-09-30 00:00:00

  • Insects and recent climate change.

    abstract：:Insects have diversified through more than 450 million y of Earth's changeable climate, yet rapidly shifting patterns of temperature and precipitation now pose novel challenges as they combine with decades of other anthropogenic stressors including the conversion and degradation of land. Here, we consider how insects ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2002543117

    authors： Halsch CA,Shapiro AM,Fordyce JA,Nice CC,Thorne JH,Waetjen DP,Forister ML

    更新日期：2021-01-12 00:00:00