Intrathymic maturation of murine T lymphocytes from CD8+ precursors.

abstract：:Nucleosomes in eukaryotes act as platforms for the dynamic integration of epigenetic information. Posttranslational modifications are reversibly added or removed and core histones exchanged for paralogous variants, in concert with changing demands on transcription and genome accessibility. Histones are also common in ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stevens KM,Swadling JB,Hocher A,Bang C,Gribaldo S,Schmitz RA,Warnecke T

更新日期：2020-12-29 00:00:00

abstract：:Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rotwein P

更新日期：1986-01-01 00:00:00

abstract：:Supernatant fractions (300,000 x g, 60 min) from homogenates of rat liver, heart, and skeletal muscle, dog liver, and rabbit liver prepared without detergent in the homogenization medium (referred to as S300) are shown to contain an activity that restores Mg2+-dependent fluoride- and guanine nucleotide-stimulated cycl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhat MK,Iyengar R,Abramowitz J,Bordelon-Riser ME,Birnbaumer L

更新日期：1980-07-01 00:00:00

abstract：:Water oxidation by cyanobacteria, algae, and plants is pivotal in oxygenic photosynthesis, the process that powers life on Earth, and is the paradigm for engineering solar fuel-production systems. Each complete reaction cycle of photosynthetic water oxidation requires the removal of four electrons and four protons fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klauss A,Haumann M,Dau H

更新日期：2012-10-02 00:00:00

abstract：:The actin cytoskeleton is a critical regulator of cytoplasmic architecture and mechanics, essential in a myriad of physiological processes. Here we demonstrate a liquid phase of actin filaments in the presence of the physiological cross-linker, filamin. Filamin condenses short actin filaments into spindle-shaped dropl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Weirich KL,Banerjee S,Dasbiswas K,Witten TA,Vaikuntanathan S,Gardel ML

更新日期：2017-02-28 00:00:00

abstract：:Our previous work [Levinger, L. & Varshavsky, A. (1982) Cell 28, 375-385] has shown that D1, a 50-kilodalton chromosomal protein of Drosophila melanogaster, is specifically associated with isolated nucleosomes that contain a complex A + T-rich satellite DNA with buoyant density of 1.688 g/ml. We show here that D1 is a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levinger L,Varshavsky A

更新日期：1982-12-01 00:00:00

abstract：:The Brazilian Amazon is one of the most rapidly developing agricultural areas in the world and represents a potentially large future source of greenhouse gases from land clearing and subsequent agricultural management. In an integrated approach, we estimate the greenhouse gas dynamics of natural ecosystems and agricul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Galford GL,Melillo JM,Kicklighter DW,Cronin TW,Cerri CE,Mustard JF,Cerri CC

更新日期：2010-11-16 00:00:00

abstract：:Site-directed spin labeling EPR (SDSL-EPR) was used to determine the structure of the inhibitory region of TnI in the intact cardiac troponin ternary complex. Maeda and collaborators have modeled the inhibitory region of TnI (skeletal 96-112: the structural motif that communicates the Ca(2+) signal to actin) as a kink...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown LJ,Sale KL,Hills R,Rouviere C,Song L,Zhang X,Fajer PG

更新日期：2002-10-01 00:00:00

abstract：:The interaction of interleukin 8 (IL-8) with heparin was studied by using synthetic IL-8 analogs with C- and N-terminal truncations. Elimination of the N-terminal region preceding the first cysteine, which constitutes the IL-8 receptor binding site, did not affect the affinity to heparin-Sepharose. Affinity, however, ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Webb LM,Ehrengruber MU,Clark-Lewis I,Baggiolini M,Rot A

更新日期：1993-08-01 00:00:00

abstract：:The very low density lipoprotein (VLDL) receptor is a recently cloned member of the low density lipoprotein (LDL) receptor family that mediates the binding and uptake of VLDL when overexpressed in animal cells. Its sequence is 94% identical in humans and rabbits and 84% identical in humans and chickens, implying a con...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Frykman PK,Brown MS,Yamamoto T,Goldstein JL,Herz J

更新日期：1995-08-29 00:00:00

abstract：:We identified a viral IL-10 homolog encoded by an ORF (UL111a) within the human cytomegalovirus (CMV) genome, which we designated cmvIL-10. cmvIL-10 can bind to the human IL-10 receptor and can compete with human IL-10 for binding sites, despite the fact that these two proteins are only 27% identical. cmvIL-10 require...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kotenko SV,Saccani S,Izotova LS,Mirochnitchenko OV,Pestka S

更新日期：2000-02-15 00:00:00

abstract：:Multiple epidemic diseases have been designated as emerging or reemerging because the numbers of clinical cases have increased. Emerging diseases are often suspected to be driven by increased virulence or fitness, possibly associated with the gain of novel genes or mutations. However, the time period over which humans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou Z,McCann A,Weill FX,Blin C,Nair S,Wain J,Dougan G,Achtman M

更新日期：2014-08-19 00:00:00

abstract：:p53 is the most frequently mutated gene in human cancers and mutant p53 has a gain of function (GOF) that promotes tumor progression and therapeutic resistance. One of the major GOF activities of mutant p53 is to suppress 2 other p53 family proteins, p63 and p73. However, the molecular basis is not fully understood. H...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang J,Sun W,Kong X,Zhang Y,Yang HJ,Ren C,Jiang Y,Chen M,Chen X

更新日期：2019-11-26 00:00:00

abstract：:The effects of phorbol 12-myristate 13-acetate (PMA), a potent activator of protein kinase C, on Na+ influx were investigated in cultured human foreskin fibroblasts (HSWP cells). We report here that in serum-deprived HSWP cells the addition of PMA alone has no significant effect on Na+ influx. However, the addition of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vicentini LM,Villereal ML

更新日期：1985-12-01 00:00:00

abstract：:The involvement of methylation in the chemosensory response of bacteria to many attractants has been clearly established by studies in several laboratories. It has been assumed that adaptation of Salmonella typhimurium and Escherichia coli to all attractants involves methylation of a transmembrane methyl-accepting che...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Niwano M,Taylor BL

更新日期：1982-01-01 00:00:00

abstract：:While the concept of intercellular mechanical communication has been revealed, the mechanistic insights have been poorly evidenced in the context of myofibroblast-fibroblast interaction during fibrosis expansion. Here we report and systematically investigate the mechanical force-mediated myofibroblast-fibroblast cross...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu L,Yu H,Zhao H,Wu Z,Long Y,Zhang J,Yan X,You Z,Zhou L,Xia T,Shi Y,Xiao B,Wang Y,Huang C,Du Y

更新日期：2020-05-19 00:00:00

abstract：:A procedure using ion exchange chromatography has been developed to detect alterations in a polysaccharide produced by Neurospora crassa. The polysaccharide, isolated from medium that has supported the growth of a culture, is highly responsive to the 3-methyl-2-benzothiazolinone hydrazone assay, indicating a high hexo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Springer WD,Srb AM

更新日期：1978-03-01 00:00:00

abstract：:Recent research on rainforest speciation has highlighted the importance of habitat variation in generating population diversification but lacks evidence of an associated reduction in gene flow. This paper describes a study in which molecular markers were used to examine the effects of allopatric divergence and habitat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ogden R,Thorpe RS

更新日期：2002-10-15 00:00:00

abstract：:Ischemia of the myocardium and lower limbs is a common consequence of arterial disease and a major source of morbidity and mortality in modernized countries. Inducing neovascularization for the treatment of ischemia is an appealing therapeutic strategy for patients for whom traditional treatment modalities cannot be p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jang E,Albadawi H,Watkins MT,Edelman ER,Baker AB

更新日期：2012-01-31 00:00:00

abstract：:We have isolated, cloned, and expressed a male antennae-specific pheromone-degrading enzyme (PDE) [Antheraea polyphemus PDE (ApolPDE), formerly known as Sensillar Esterase] from the wild silkmoth, A. polyphemus, which seems essential for the rapid inactivation of pheromone during flight. The onset of enzymatic activit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ishida Y,Leal WS

更新日期：2005-09-27 00:00:00

abstract：:It has long been recognized that the renal proximal tubular epithelium of the hamster is a bona fide estrogen target tissue. The effect of estrogens on the growth of proximal tubule cell explants and dissociated single cells derived from these explant outgrowths has been studied in culture. Renal tubular cells were gr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oberley TD,Lauchner LJ,Pugh TD,Gonzalez A,Goldfarb S,Li SA,Li JJ

更新日期：1989-03-01 00:00:00

abstract：:Infection of newborn rats with Borne disease virus (BDV) results in selective degeneration of granule cell neurons of the dentate gyrus (DG). To study cellular countermechanisms that might prevent this pathology, we screened for rat strains resistant to this BDV-induced neuronal degeneration. To this end, we infected ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu YJ,Schulz H,Lin CC,Saar K,Patone G,Fischer H,Hübner N,Heimrich B,Schwemmle M

更新日期：2013-01-29 00:00:00

abstract：:To understand the biogeochemical roles of microorganisms in the environment, it is important to determine when and under which conditions they are metabolically active. Bioorthogonal noncanonical amino acid tagging (BONCAT) can reveal active cells by tracking the incorporation of synthetic amino acids into newly synth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hatzenpichler R,Connon SA,Goudeau D,Malmstrom RR,Woyke T,Orphan VJ

更新日期：2016-07-12 00:00:00

abstract：:Nations in the 21st century face a complex mix of environmental and social challenges, as highlighted by the on-going Sustainable Development Goals process. The "planetary boundaries" concept [Rockström J, et al. (2009) Nature 461(7263):472-475], and its extension through the addition of social well-being indicators t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cole MJ,Bailey RM,New MG

更新日期：2014-10-21 00:00:00

abstract：:Programmed cell death (apoptosis) is a normal physiological process, which could in principle be manipulated to play an important role in cancer therapy. The key importance of p53 expression in the apoptotic response to DNA-damaging agents has been stressed because mutant or deleted p53 is so common in most kinds of c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu C,Meikrantz W,Schlegel R,Sager R

更新日期：1995-08-15 00:00:00

abstract：:The multisubunit enzyme aspartate transcarbamylase consists of six copies of two types of polypeptide chains, catalytic (C) and regulatory (R). A complex formed by the partial dissociation of this enzyme has been isolated. This species, which has the structure C(6)R(4), is a likely intermediate in the stepwise dissoci...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Evans DR,Pastra-Landis SC,Lipscomb WN

更新日期：1974-04-01 00:00:00

abstract：:For decades, scientists have debated whether supercooled liquids stop flowing below a glass transition temperature [Formula: see text] or whether motion continues to slow gradually down to zero temperature. Answering this question is challenging because human time scales set a limit on the largest measurable viscosity...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jadhao V,Robbins MO

更新日期：2017-07-25 00:00:00

abstract：:Inosine monophosphate dehydrogenase (IMPDH) controls a key metabolic step in the regulation of cell growth and differentiation. This step is the NAD-dependent oxidation of inosine 5' monophosphate (IMP) to xanthosine 5' monophosphate, the rate-limiting step in the synthesis of the guanine nucleotides. Two isoforms of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colby TD,Vanderveen K,Strickler MD,Markham GD,Goldstein BM

更新日期：1999-03-30 00:00:00

abstract：:Several tissue culture cell lines that were transformed by a tumor virus have been found to react with an agglutinin, while under identical conditions their untransformed parent cell lines did not agglutinate. Since a short treatment of the parent cell line with low concentrations of proteases exposed the same aggluti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Burger MM

更新日期：1969-03-01 00:00:00

abstract：:We investigated the effect of actin filament barbed end uncapping on Arp23 complex function both in vivo and in vitro. Arp23 complex redistributes rapidly and uniformly to the lamellar edge of activated wild-type platelets and fibroblasts but clusters in marginal actin filament clumps in gelsolin-null cells. Treatment...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Falet H,Hoffmeister KM,Neujahr R,Italiano JE Jr,Stossel TP,Southwick FS,Hartwig JH

更新日期：2002-12-24 00:00:00