Measuring single-cell density.

Abstract:

:We have used a microfluidic mass sensor to measure the density of single living cells. By weighing each cell in two fluids of different densities, our technique measures the single-cell mass, volume, and density of approximately 500 cells per hour with a density precision of 0.001 g mL(-1). We observe that the intrinsic cell-to-cell variation in density is nearly 100-fold smaller than the mass or volume variation. As a result, we can measure changes in cell density indicative of cellular processes that would be otherwise undetectable by mass or volume measurements. Here, we demonstrate this with four examples: identifying Plasmodium falciparum malaria-infected erythrocytes in a culture, distinguishing transfused blood cells from a patient's own blood, identifying irreversibly sickled cells in a sickle cell patient, and identifying leukemia cells in the early stages of responding to a drug treatment. These demonstrations suggest that the ability to measure single-cell density will provide valuable insights into cell state for a wide range of biological processes.

authors

Grover WH,Bryan AK,Diez-Silva M,Suresh S,Higgins JM,Manalis SR

doi

10.1073/pnas.1104651108

subject

Has Abstract

pub_date

2011-07-05 00:00:00

pages

10992-6

issue

27

eissn

0027-8424

issn

1091-6490

pii

1104651108

journal_volume

108

pub_type

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