Design of a mechanical clutch-based needle-insertion device.

abstract：:The embryonic sites in which progenitors of the hematopoietic lineages first emerge are ideal regions to characterize both the cells and environment needed to initiate blood cell development. For a number of years both the murine yolk sac and embryo have been recognized to contain progenitors of B lymphocytes. However...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cumano A,Furlonger C,Paige CJ

更新日期：1993-07-15 00:00:00

abstract：:[3H]Puromycin and N-(ethyl-2-diazomalonyl)[3H]puromycin are incorporated into E. coli ribosomes on irradiation at 253.7 nm. Both compounds incorporate into both protein and nucleic acid. Two-dimensional gel electrophoresis of ribosomal protein shows that L23 is the major protein labeled by puromycin. Although incorpor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cooperman BS,Jaynes EN,Brunswick DJ,Luddy MA

更新日期：1975-08-01 00:00:00

abstract：:A unified structure theory of icosahedral quasicrystals, combining the twinned-cubic-crystal theory and the Penrose-tiling-six-dimensional-projection theory, is described. Values of the primitive-cubic lattice constant for several quasicrystals are evaluated from x-ray and neutron diffraction data. The fact that the l...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pauling L

更新日期：1988-11-01 00:00:00

abstract：:Pertussis toxin (PT), a protein produced by Bordetella pertussis, was studied for its effect on lipolysis in isolated rat epididymal adipocytes. Exposure of adipocytes to pertussis toxin resulted in a significant increase in cyclic AMP levels and lipolysis after a lag of 1-2 hr. Both the maximal rate of lipolysis and ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olansky L,Myers GA,Pohl SL,Hewlett EL

更新日期：1983-11-01 00:00:00

abstract：:Misalignment between internal circadian rhythmicity and externally imposed behavioral schedules, such as occurs in shift workers, has been implicated in elevated risk of metabolic disorders. To determine underlying mechanisms, it is essential to assess whether and how peripheral clocks are disturbed during shift work ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Skene DJ,Skornyakov E,Chowdhury NR,Gajula RP,Middleton B,Satterfield BC,Porter KI,Van Dongen HPA,Gaddameedhi S

更新日期：2018-07-24 00:00:00

abstract：:Increased cardiovascular mortality occurs in diabetic patients with or without coronary artery disease and is attributed to the presence of diabetic cardiomyopathy. One potential mechanism is hyperglycemia that has been reported to activate protein kinase C (PKC), preferentially the beta isoform, which has been associ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wakasaki H,Koya D,Schoen FJ,Jirousek MR,Ways DK,Hoit BD,Walsh RA,King GL

更新日期：1997-08-19 00:00:00

abstract：:A monomorphic anti-HLA-G monoclonal antibody (mAb) was obtained by immunization of HLA-B27/human beta 2-microglobulin double-transgenic mice with transfected murine L cells expressing both HLA-G and human beta 2-microglobulin. This mAb, designated BFL.1, specifically recognizes, by flow cytometry analysis, the immuniz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bensussan A,Mansur IG,Mallet V,Rodriguez AM,Girr M,Weiss EH,Brem G,Boumsell L,Gluckman E,Dausset J

更新日期：1995-10-24 00:00:00

abstract：:30S ribosomal proteins from Bacillus stearothermophilus (B. proteins) have been fractionated and characterized with respect to their ability to replace various E. coli 30S proteins (E. proteins) in the E. coli 30S ribosome reconstitution system. The functional counterparts of all the E. proteins, except S1, S6, S9, an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higo K,Held W,Kahan L,Nomura M

更新日期：1973-03-01 00:00:00

abstract：:Thymic epithelial cells (TECs), the major component of the thymic microenvironment, can be modulated by pituitary hormones. We have shown previously that prolactin (PRL) can influence the endocrine activity of TECs and stimulate TEC proliferation as well as cytokeratin expression, suggesting the existence of PRL recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dardenne M,Kelly PA,Bach JF,Savino W

更新日期：1991-11-01 00:00:00

abstract：:Relatively little is known about neurobiological changes attributable to early-life stressors (e.g., orphanage rearing), even though they have been associated with a heightened risk for later psychopathology. Human neuroimaging and animal studies provide complementary insights into the neural basis of problem behavior...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malter Cohen M,Jing D,Yang RR,Tottenham N,Lee FS,Casey BJ

更新日期：2013-11-05 00:00:00

abstract：:Many first-line cancer drugs are natural products or are derived from them by chemical modification. The trioxacarcins are an emerging class of molecules of microbial origin with potent antiproliferative effects, which may derive from their ability to covalently modify duplex DNA. All trioxacarcins appear to be deriva...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Švenda J,Hill N,Myers AG

更新日期：2011-04-26 00:00:00

abstract：:Representations of scientific knowledge must reflect the dynamic nature of knowledge construction and the evolving networks of relations between scientific concepts. In this article, we describe initial work toward dynamic, visual methods and tools that support the construction, communication, revision, and applicatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： MacEachren AM,Gahegan M,Pike W

更新日期：2004-04-06 00:00:00

abstract：:The rotational strengths of the four longer wavelength transitions, B(2u), B(1u), and the two E(1u), of adenosine derivatives constrained in the syn form have been investigated theoretically and experimentally. The theory combines a complete neglect of differential overlap version S (CNDO/S) description of the base wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles DW,Farmer M,Eyring H

更新日期：1980-06-01 00:00:00

abstract：:Adaptation to hypoxia is mediated by transactivation of hypoxia-responsive genes by hypoxia-inducible factor-1 (HIF-1) in complex with the CBP and p300 transcriptional coactivators. We report the solution structure of the cysteine/histidine-rich 1 (CH1) domain of p300 bound to the C-terminal transactivation domain of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Freedman SJ,Sun ZY,Poy F,Kung AL,Livingston DM,Wagner G,Eck MJ

更新日期：2002-04-16 00:00:00

abstract：:A purified rabbit liver membrane protein that binds desialylated glycoproteins has been shown to be a mitogen for human peripheral lymphocytes. The mitogenic activity is specific for desialylated thymus-derived (T)-cells. The loss of mitogenicity upon exposure of the binding protein to neuraminidase and the inhibitory...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Novogrodsky A,Ashwell G

更新日期：1977-02-01 00:00:00

abstract：:ATP-sensitive potassium channels (K(ATP)) are involved in a diverse array of physiologic functions including protection of tissue against ischemic insult, regulation of vascular tone, and modulation of insulin secretion. To improve our understanding of the role of K(ATP) in these processes, we used a gene-targeting st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chutkow WA,Samuel V,Hansen PA,Pu J,Valdivia CR,Makielski JC,Burant CF

更新日期：2001-09-25 00:00:00

abstract：:Interleukin 3 (IL-3) is a hematopoietic growth factor that supports the proliferation and differentiation of early hematopoietic-lineage progenitors. By using growth factor-dependent cell lines, IL-3 has been shown to induce the appearance of several phosphotyrosine-containing proteins, including a 140-kDa cell surfac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Isfort RJ,Stevens D,May WS,Ihle JN

更新日期：1988-11-01 00:00:00

abstract：:Forkhead box class O (FoxO) transcription factors are key regulators of growth, metabolism, life span, and stress resistance. FoxOs integrate signals from different pathways and guide the cellular response to varying energy and stress conditions. FoxOs are modulated by several signaling pathways, e.g., the insulin-TOR...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mattila J,Kallijärvi J,Puig O

更新日期：2008-09-30 00:00:00

abstract：:Dual-color fluorescence cross-correlation spectroscopy (dual-color FCS) has previously been shown to be a suitable tool not only for binding but also for catalytic rate studies. In this work, its application as a rapid method for high-throughput screening (HTS) and evolutionary biotechnology is described. This applica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koltermann A,Kettling U,Bieschke J,Winkler T,Eigen M

更新日期：1998-02-17 00:00:00

abstract：:Cities are concentrated areas of CO2 emissions and have become the foci of policies for mitigation actions. However, atmospheric measurement networks suitable for evaluating urban emissions over time are scarce. Here we present a unique long-term (decadal) record of CO2 mole fractions from five sites across Utah's met...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell LE,Lin JC,Bowling DR,Pataki DE,Strong C,Schauer AJ,Bares R,Bush SE,Stephens BB,Mendoza D,Mallia D,Holland L,Gurney KR,Ehleringer JR

更新日期：2018-03-20 00:00:00

abstract：:Allostatic load (AL) is a measure of overall physiological wear-and-tear over the life course, which could partially be the consequence of early life exposures. AL could allow a better understanding of the potential biological pathways playing a role in the construction of the social gradient in adult health. To explo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barboza Solís C,Kelly-Irving M,Fantin R,Darnaudéry M,Torrisani J,Lang T,Delpierre C

更新日期：2015-02-17 00:00:00

abstract：:Human infections with Ebola virus (EBOV) result in a deadly viral disease known as Ebola hemorrhagic fever. Up to 90% of infected patients die, and there is no available treatment or vaccine. The sporadic human outbreaks are believed to result when EBOV "jumps" from an infected animal to a person and is subsequently t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Strong JE,Wong G,Jones SE,Grolla A,Theriault S,Kobinger GP,Feldmann H

更新日期：2008-11-18 00:00:00

abstract：:The transporter associated with antigen processing (TAP) is essential for intracellular transport of protein fragments into the endoplasmic reticulum for loading of major histocompatibility complex (MHC) class I molecules. On the cell surface, these peptide-MHC complexes are monitored by cytotoxic T lymphocytes. To st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gorbulev S,Abele R,Tampé R

更新日期：2001-03-27 00:00:00

abstract：:Several groups have attempted to develop gene therapy strategies to treat cancer via introduction of the wild-type (wt) p53 cDNA into cancer cells. Unfortunately, these approaches do not result in regulated expression of the p53 gene and do not reduce expression of the mutant p53 that is overexpressed in cancerous cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe T,Sullenger BA

更新日期：2000-07-18 00:00:00

abstract：:Defining the processes of autoimmune attack of tissues is important for inhibiting continued tissue destruction. In type 1 diabetes, it is not known how cytotoxic effector T cell responses evolve over time in the pancreatic islets targeted for destruction. We used two-photon microscopy of live, intact, individual isle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedman RS,Lindsay RS,Lilly JK,Nguyen V,Sorensen CM,Jacobelli J,Krummel MF

更新日期：2014-06-24 00:00:00

abstract：:Factor VIII (antihemophilic factor) is a high molecular weight plasma glycoprotein that participates in the blood clotting cascade. The recent cloning and sequence analysis of the cDNA encoding human factor VIII revealed an obvious domain structure for the protein, which can be represented as A1-A2-B-A3-C1-C2. We now ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toole JJ,Pittman DD,Orr EC,Murtha P,Wasley LC,Kaufman RJ

更新日期：1986-08-01 00:00:00

abstract：:Reward-guided decision-making depends on a network of brain regions. Among these are the orbitofrontal and the anterior cingulate cortex. However, it is difficult to ascertain if these areas constitute anatomical and functional unities, and how these areas correspond between monkeys and humans. To address these questi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Neubert FX,Mars RB,Sallet J,Rushworth MF

更新日期：2015-05-19 00:00:00

abstract：:In response to vascular injury, vascular smooth muscle cells (VSMCs) may switch from a contractile to a proliferative phenotype thereby contributing to neointima formation. Previous studies showed that the long noncoding RNA (lncRNA) NEAT1 is critical for paraspeckle formation and tumorigenesis by promoting cell proli...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahmed ASI,Dong K,Liu J,Wen T,Yu L,Xu F,Kang X,Osman I,Hu G,Bunting KM,Crethers D,Gao H,Zhang W,Liu Y,Wen K,Agarwal G,Hirose T,Nakagawa S,Vazdarjanova A,Zhou J

更新日期：2018-09-11 00:00:00

abstract：:Evolution of new enzymatic activities is believed to require a period of gene sharing in which a single enzyme must serve both its original function and a new function that has become advantageous to the organism. Subsequent gene duplication allows one copy to maintain the original function, while the other diverges t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McLoughlin SY,Copley SD

更新日期：2008-09-09 00:00:00

abstract：:Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A "code" for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cheong CG,Hall TM

更新日期：2006-09-12 00:00:00