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Engineering RNA sequence specificity of Pumilio repeats.

Abstract：

:Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A "code" for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here, we report the creation of seven soluble mutant proteins with predictably altered sequence specificity, including one that binds tightly to adenosine-uracil-rich element RNA. These data show that Pumilio1 can be used as a scaffold to engineer RNA-binding proteins with designed sequence specificity.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Cheong CG,Hall TM

doi

10.1073/pnas.0606294103

subject

Has Abstract

pub_date

2006-09-12 00:00:00

pages

13635-9

issue

37

eissn

0027-8424

issn

1091-6490

pii

0606294103

journal_volume

103

pub_type

杂志文章

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