A large region (approximately equal to 95 kDa) of human factor VIII is dispensable for in vitro procoagulant activity.

abstract：:ALS is a terminal disease of motor neurons that is characterized by accumulation of proteinaceous deposits in affected cells. Pathological deposition of mutated Cu/Zn superoxide dismutase (SOD1) accounts for ∼20% of the familial ALS (fALS) cases. However, understanding the molecular link between mutation and disease h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ivanova MI,Sievers SA,Guenther EL,Johnson LM,Winkler DD,Galaleldeen A,Sawaya MR,Hart PJ,Eisenberg DS

更新日期：2014-01-07 00:00:00

abstract：:The transcription factor caudal-type homeobox 1 (CDX1) is a key regulator of differentiation in the normal colon and in colorectal cancer (CRC). CDX1 activates the expression of enterocyte genes, but it is not clear how the concomitant silencing of stem cell genes is achieved. MicroRNAs (miRNAs) are important mediator...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jones MF,Hara T,Francis P,Li XL,Bilke S,Zhu Y,Pineda M,Subramanian M,Bodmer WF,Lal A

更新日期：2015-03-31 00:00:00

abstract：:Myosins play countless critical roles in the cell, each requiring it to be activated at a specific location and time. To control myosin VI with this specificity, we created an optogenetic tool for activating myosin VI by fusing the light-sensitive Avena sativa phototropin1 LOV2 domain to a peptide from Dab2 (LOVDab), ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： French AR,Sosnick TR,Rock RS

更新日期：2017-02-28 00:00:00

abstract：:Cyclic electron flow is increasingly recognized as being essential in plant growth, generating a pH gradient across thylakoid membrane (ΔpH) that contributes to ATP synthesis and triggers the protective process of nonphotochemical quenching (NPQ) under stress conditions. Here, we report experiments demonstrating the i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Joliot P,Johnson GN

更新日期：2011-08-09 00:00:00

abstract：:The great mass extinctions of the fossil record were a major creative force that provided entirely new kinds of opportunities for the subsequent explosive evolution and diversification of surviving clades. Today, the synergistic effects of human impacts are laying the groundwork for a comparably great Anthropocene mas...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jackson JB

更新日期：2008-08-12 00:00:00

abstract：:The structure and the expression of 11 cellular oncogenes (protooncogenes) were analyzed in primary cells from 20 acute lymphocytic (ALL) and 31 acute myelogenous (AML) leukemia patients. Neoplastic cells, obtained prior to initiation of therapy, were purified and classified, on the basis of both surface antigen patte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mavilio F,Sposi NM,Petrini M,Bottero L,Marinucci M,De Rossi G,Amadori S,Mandelli F,Peschle C

更新日期：1986-06-01 00:00:00

abstract：:High sensitivity differential scanning calorimetry is applied to the study of the thermotropic behavior of mixtures of synthetic phospholipids in multilamellar aqueous suspensions. The systems dimyristoylphosphatidylcholine dipalmitoylphosphatidylcholine, and dimyristoylphosphatidylethanolamine-distearoylphosphatidyl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mabrey S,Sturtevant JM

更新日期：1976-11-01 00:00:00

abstract：:MicroRNAs (miRNAs) play essential, conserved roles in diverse developmental processes through association with the miRNA-induced silencing complex (miRISC). Whereas fundamental insights into the mechanistic framework of miRNA biogenesis and target gene silencing have been established, posttranslational modifications t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alessi AF,Khivansara V,Han T,Freeberg MA,Moresco JJ,Tu PG,Montoye E,Yates JR 3rd,Karp X,Kim JK

更新日期：2015-12-29 00:00:00

abstract：:Dysregulation of cyclin-dependent kinase 5 (cdk5) per relative concentrations of its activators p35 and p25 is implicated in neurodegenerative diseases. P35 has a short t½ and undergoes rapid proteasomal degradation in its membrane-bound myristoylated form. P35 is converted by calpain to p25, which, along with an exte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai SY,Pokrass MJ,Klauer NR,Nohara H,Su TP

更新日期：2015-05-26 00:00:00

abstract：:Quinate:NAD(+) 3-oxidoreductase (EC 1.1.1.24) from carrot cell suspension cultures has previously been shown to be activated by phosphorylation and inactivated by dephosphorylation. Here it is shown that the reactivation of the inactivated quinate:NAD(+) oxidoreductase is an enzyme-mediated process that requires ATP a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ranjeva R,Refeno G,Boudet AM,Marmé D

更新日期：1983-09-01 00:00:00

abstract：:Mesothelin is a differentiation antigen present on the surface of ovarian cancers, mesotheliomas, and several other types of human cancers. Because among normal tissues, mesothelin is present only on mesothelial cells, it represents a good target for antibody-mediated delivery of cytotoxic agents. In the present study...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chowdhury PS,Viner JL,Beers R,Pastan I

更新日期：1998-01-20 00:00:00

abstract：:Extreme animal movements are usually associated with a single, high-performance behavior. However, the remarkably rapid mandible strikes of the trap-jaw ant, Odontomachus bauri, can yield multiple functional outcomes. Here we investigate the biomechanics of mandible strikes in O. bauri and find that the extreme mandib...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patek SN,Baio JE,Fisher BL,Suarez AV

更新日期：2006-08-22 00:00:00

abstract：:The Escherichia coli cytosolic homotetrameric protein SecB is known to be involved in protein export across the plasma membrane. A currently prevalent view holds that SecB functions exclusively as a chaperone interacting nonspecifically with unfolded proteins, not necessarily exported proteins, whereas a contrary view...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe M,Blobel G

更新日期：1995-10-24 00:00:00

abstract：:Tunas are migratory fishes in offshore habitats and top predators with unique features. Despite their ecological importance and high market values, the open-ocean lifestyle of tuna, in which effective sensing systems such as color vision are required for capture of prey, has been poorly understood. To elucidate the ge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakamura Y,Mori K,Saitoh K,Oshima K,Mekuchi M,Sugaya T,Shigenobu Y,Ojima N,Muta S,Fujiwara A,Yasuike M,Oohara I,Hirakawa H,Chowdhury VS,Kobayashi T,Nakajima K,Sano M,Wada T,Tashiro K,Ikeo K,Hattori M,Kuhara S,

更新日期：2013-07-02 00:00:00

abstract：:The cytokines interleukin (IL) 4 and IL-13 induce many of the same biological responses, including class switching to IgE and induction of major histocompatibility complex class II antigens and CD23 on human B cells. It has recently been shown that IL-4 induces the tyrosine phosphorylation of a 170-kDa protein, a subs...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Keegan AD,Johnston JA,Tortolani PJ,McReynolds LJ,Kinzer C,O'Shea JJ,Paul WE

更新日期：1995-08-15 00:00:00

abstract：:Whether G protein-coupled receptors signal from endosomes to control important pathophysiological processes and are therapeutic targets is uncertain. We report that opioids from the inflamed colon activate δ-opioid receptors (DOPr) in endosomes of nociceptors. Biopsy samples of inflamed colonic mucosa from patients an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jimenez-Vargas NN,Gong J,Wisdom MJ,Jensen DD,Latorre R,Hegron A,Teng S,DiCello JJ,Rajasekhar P,Veldhuis NA,Carbone SE,Yu Y,Lopez-Lopez C,Jaramillo-Polanco J,Canals M,Reed DE,Lomax AE,Schmidt BL,Leong KW,Vanner SJ,

更新日期：2020-06-30 00:00:00

abstract：:A full-length placental alkaline phosphatase (PLAP) cDNA was used to identify and clone the PLAP-like Nagao isozyme gene from human genomic libraries. The entire nucleotide sequence of the gene reveals the existence of 11 exons interrupted by 10 small introns (76-427 base pairs). Putative regulatory sequences have bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Millán JL,Manes T

更新日期：1988-05-01 00:00:00

abstract：:The exceptional sensitivity of Mycobacterium tuberculosis to isonicotinic acid hydrazide (INH) lacks satisfactory definition. M. tuberculosis is a natural mutant in oxyR, a central regulator of peroxide stress response. The ahpC gene, which encodes a critical subunit of alkyl hydroperoxide reductase, is one of the tar...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Dhandayuthapani S,Deretic V

更新日期：1996-11-12 00:00:00

abstract：:Accumulating evidence suggests that α-synuclein (α-syn) occurs physiologically as a helically folded tetramer that resists aggregation. However, the mechanisms underlying the regulation of formation of α-syn tetramers are still mostly unknown. Cellular membrane lipids are thought to play an important role in the regul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kim S,Yun SP,Lee S,Umanah GE,Bandaru VVR,Yin X,Rhee P,Karuppagounder SS,Kwon SH,Lee H,Mao X,Kim D,Pandey A,Lee G,Dawson VL,Dawson TM,Ko HS

更新日期：2018-01-23 00:00:00

abstract：:Mammalian CLC proteins function as Cl(-) channels or as electrogenic Cl(-)/H(+) exchangers and are present in the plasma membrane and intracellular vesicles. We now show that the ClC-6 protein is almost exclusively expressed in neurons of the central and peripheral nervous systems, with a particularly high expression ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poët M,Kornak U,Schweizer M,Zdebik AA,Scheel O,Hoelter S,Wurst W,Schmitt A,Fuhrmann JC,Planells-Cases R,Mole SE,Hübner CA,Jentsch TJ

更新日期：2006-09-12 00:00:00

abstract：:The functional significance of the portal vascular link between the beta cells of the pancreatic islets and the liver has not been established. Previous studies indicated that insulin does not acutely regulate glucose metabolism by a direct hepatic effect. More recent observations suggest that the role of insulin in r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Griffen SC,Russell SM,Katz LS,Nicoll CS

更新日期：1987-10-01 00:00:00

abstract：:Normal human cells whether embryonic, neonatal, or adult are resistant to experimentally induced tumorigenesis in contrast to rodent or chicken cells. We showed previously that neither transformation with simian virus 40 DNA nor transfection with human mutant HRAS DNA immortalized FS-2 cells (diploid, neonatal human f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Brien W,Stenman G,Sager R

更新日期：1986-11-01 00:00:00

abstract：:Theory predicts that double-core-hole (DCH) spectroscopy can provide a new powerful means of differentiating between similar chemical systems with a sensitivity not hitherto possible. Although DCH ionization on a single site in molecules was recently measured with double- and single-photon absorption, double-core hole...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Berrah N,Fang L,Murphy B,Osipov T,Ueda K,Kukk E,Feifel R,van der Meulen P,Salen P,Schmidt HT,Thomas RD,Larsson M,Richter R,Prince KC,Bozek JD,Bostedt C,Wada S,Piancastelli MN,Tashiro M,Ehara M

更新日期：2011-10-11 00:00:00

abstract：:Antigenic variation of infectious organisms is a major factor in evasion of the host immune response. However, there has been no definitive demonstration of this phenomenon in the malaria parasite Plasmodium falciparum. In this study, cloned parasites were examined serologically and biochemically for the expression of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Biggs BA,Goozé L,Wycherley K,Wollish W,Southwell B,Leech JH,Brown GV

更新日期：1991-10-15 00:00:00

abstract：:Achieving long-term pancellular expression of a transferred gene at therapeutic level in a given hematopoietic lineage remains an important goal of gene therapy. Advances have recently been made in the genetic correction of the hemoglobinopathies by means of lentiviral vectors and large locus control region (LCR) deri...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Imren S,Payen E,Westerman KA,Pawliuk R,Fabry ME,Eaves CJ,Cavilla B,Wadsworth LD,Beuzard Y,Bouhassira EE,Russell R,London IM,Nagel RL,Leboulch P,Humphries RK

更新日期：2002-10-29 00:00:00

abstract：:The tumor suppressor activity of p53 is regulated by interactions with the ubiquitin ligase HDM2 and the general transcriptional coactivators CBP and p300. Using NMR spectroscopy and isothermal titration calorimetry, we have dissected the binding interactions between the N-terminal transactivation domain (TAD) of p53,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferreon JC,Lee CW,Arai M,Martinez-Yamout MA,Dyson HJ,Wright PE

更新日期：2009-04-21 00:00:00

abstract：:The highly ordered and reproducible structure of the fungal prion HET-s makes it an excellent model system for studying the inherent properties of prions, self-propagating infectious proteins that have been implicated in a number of fatal diseases. In particular, the HET-s prion-forming domain readily folds into a rel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wan W,Stubbs G

更新日期：2014-04-08 00:00:00

abstract：:Natural killer T (NKT) cells provide an innate-type immune response upon T cell receptor interaction with CD1d-presented antigens. We demonstrate through equilibrium tetramer binding and antigen presentation assays with Valpha14i-positive NKT cell hybridomas that the Sphingomonas glycolipid alpha-galacturonosyl cerami...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu D,Zajonc DM,Fujio M,Sullivan BA,Kinjo Y,Kronenberg M,Wilson IA,Wong CH

更新日期：2006-03-14 00:00:00

abstract：:Prions are mammalian proteins (PrPs) with a unique pathogenic property: a nonendogenous isoform PrP(Sc) can catalyze conversion of the endogenous PrP(C) isoform into additional PrP(Sc). In this work, we demonstrate that PrP(C) helix 1 has certain properties (hydrophilicity, charge distribution) that make it unique amo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morrissey MP,Shakhnovich EI

更新日期：1999-09-28 00:00:00

abstract：:Platelet-derived growth factor receptor (PDGFR) signaling is essential for normal embryonic development in many organisms, including frog, mouse, zebrafish, and sea urchin. The mode of action of PDGFR signaling during early development is poorly understood, however, mostly because inhibition of signaling through eithe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Van Stry M,Kazlauskas A,Schreiber SL,Symes K

更新日期：2005-06-07 00:00:00