Distinct effectors of platelet-derived growth factor receptor-alpha signaling are required for cell survival during embryogenesis.

abstract：:One of the advantages of the production of monoclonal antibodies by tissue culture methods is that they can be internally labeled by using appropriate radioactive amino acid in the culture fluid. Thus, radioactive immunological probes of high specific activity can be prepared. Here we report applications of these inte...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cuello AC,Priestley JV,Milstein C

更新日期：1982-01-01 00:00:00

abstract：:Do negative feelings in general trigger addictive behavior, or do specific emotions play a stronger role? Testing these alternative accounts of emotion and decision making, we drew on the Appraisal Tendency Framework to predict that sadness, specifically, rather than negative mood, generally, would 1) increase craving...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dorison CA,Wang K,Rees VW,Kawachi I,Ericson KMM,Lerner JS

更新日期：2020-01-14 00:00:00

abstract：:CDK7 is a cyclin-dependent kinase proposed to function in two essential cellular processes: transcription and cell cycle regulation. CDK7 is the kinase subunit of the general transcription factor TFIIH that phosphorylates the C-terminal domain (CTD) of RNA polymerase II, and has been shown to be broadly required for t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallenfang MR,Seydoux G

更新日期：2002-04-16 00:00:00

abstract：:The het-s locus of Podospora anserina is a heterokaryon incompatibility locus. The coexpression of the antagonistic het-s and het-S alleles triggers a lethal reaction that prevents the formation of viable heterokaryons. Strains that contain the het-s allele can display two different phenotypes, [Het-s] or [Het-s*], ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Coustou V,Deleu C,Saupe S,Begueret J

更新日期：1997-09-02 00:00:00

abstract：:Constitutive cyclooxygenase (COX-1; prostaglandin-endoperoxide synthase, EC 1.14.99.1) is present in cells under physiological conditions, whereas COX-2 is induced by some cytokines, mitogens, and endotoxin presumably in pathological conditions, such as inflammation. Therefore, we have assessed the relative inhibitory...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitchell JA,Akarasereenont P,Thiemermann C,Flower RJ,Vane JR

更新日期：1993-12-15 00:00:00

abstract：:Visual deficits caused by abnormal visual experience during development are hard to recover in adult animals. Removal of chondroitin sulfate proteoglycans from the mature extracellular matrix with chondroitinase ABC promotes plasticity in the adult visual cortex. We tested whether chondroitinase ABC treatment of adult...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pizzorusso T,Medini P,Landi S,Baldini S,Berardi N,Maffei L

更新日期：2006-05-30 00:00:00

abstract：:We report direct evidence for the existence of an iron(IV)-hydroxide. Resonance Raman measurements on chloroperoxidase compound II (CPO-II) reveal an isotope ((18)O and (2)H)-sensitive band at nu(Fe-O) = 565 cm(-1). Preparation of CPO-II in H(2)O using H(2)(18)O(2) results in a red-shift of 22 cm(-1), while preparatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stone KL,Behan RK,Green MT

更新日期：2006-08-15 00:00:00

abstract：:Clostridium difficile, a causative agent of antibiotic-associated diarrhea and its potentially lethal form, pseudomembranous colitis, produces two large protein toxins that are responsible for the cellular damage associated with the disease. The level of toxin production appears to be critical for determining the seve...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mani N,Dupuy B

更新日期：2001-05-08 00:00:00

abstract：:30S ribosomal proteins from Bacillus stearothermophilus (B. proteins) have been fractionated and characterized with respect to their ability to replace various E. coli 30S proteins (E. proteins) in the E. coli 30S ribosome reconstitution system. The functional counterparts of all the E. proteins, except S1, S6, S9, an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Higo K,Held W,Kahan L,Nomura M

更新日期：1973-03-01 00:00:00

abstract：:The fdhF gene encoding the 80-kDa selenopolypeptide subunit of formate dehydrogenase H from Escherichia coli contains an in-frame TGA codon at amino acid position 140, which encodes selenocysteine. We have analyzed how this UGA "sense codon" is discriminated from a UGA codon signaling polypeptide chain termination. De...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zinoni F,Heider J,Böck A

更新日期：1990-06-01 00:00:00

abstract：:The histidine decarboxylase (L-histidine carboxylase, EC 4.1.1.22) activity of whole W/W mouse embryos, which are devoid of mast cells, remained very low and did not show the rapid increase before birth (17-20 days of gestation) seen in wild-type +/+ embryos. During the same period, the histamine content also remained...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe T,Kitamura Y,Maeyama K,Go S,Yamatodani A,Wada H

更新日期：1981-07-01 00:00:00

abstract：:Mutants of Escherichia coli sensitive to phage T4 gene 2 mutants were obtained following ethyl methanesulfonate mutagenesis. By mapping and complementation analysis, the mutations in each of the six mutants are in recB and recC. By both in vivo and in vitro analyses, the nuclease activity of RecBC enzyme is undetectab...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chaudhury AM,Smith GR

更新日期：1984-12-01 00:00:00

abstract：:Global energy demand and environmental concerns have stimulated increasing efforts to produce carbon-neutral fuels directly from renewable resources. Microbially derived aliphatic hydrocarbons, the petroleum-replica fuels, have emerged as promising alternatives to meet this goal. However, engineering metabolic pathway...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu P,Li L,Zhang F,Stephanopoulos G,Koffas M

更新日期：2014-08-05 00:00:00

abstract：:Future nuclear arms reduction efforts will require technologies to verify that warheads slated for dismantlement are authentic without revealing any sensitive weapons design information to international inspectors. Despite several decades of research, no technology has met these requirements simultaneously. Recent wor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vavrek JR,Henderson BS,Danagoulian A

更新日期：2018-04-24 00:00:00

abstract：:Nonesterified fatty acids accumulate at sites of tissue injury and necrosis. In cardiac tissue the concentrations of oleic acid, arachidonic acid, leukotrienes, and other fatty acids increase greatly during ischemia due to receptor or nonreceptor-mediated activation of phospholipases and/or diminished reacylation. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang JM,Xian H,Bacaner M

更新日期：1992-07-15 00:00:00

abstract：:The role of cell surface immunoglobulin in helper T-cell-dependent B-cell activation was analyzed using a B-cell lymphoma, CH12, with known antigen specificity and activation properties similar to those of a resting B cell. Two sources of helper T cells were used, both selected such that they interact with H-2-encoded...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： LoCascio NJ,Haughton G,Arnold LW,Corley RB

更新日期：1984-04-01 00:00:00

abstract：:Ras is a guanine nucleotide-binding protein that cycles between inactive GDP-bound and active GTP-bound states to regulate a diverse array of cellular processes, including cell growth, apoptosis, and differentiation. The guanine nucleotide-bound state of Ras is tightly maintained by regulatory factors to promote regul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Williams JG,Pappu K,Campbell SL

更新日期：2003-05-27 00:00:00

abstract：:Normal rat kidney cells were nonproductively infected either with CP27, a mutant of Moloney sarcoma virus that is temperature-sensitive for maintenance of transformation, or with the parental wild-type virus. The nonproducer cells were inoculated into the tails of athymic nude mice that were subsequently incubated at ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Klarlund JK,Forchhammer J

更新日期：1980-03-01 00:00:00

abstract：:In the search for selective and long-acting analogs of somatostatin, nearly 200 compounds were synthesized by solid-phase methods, purified, and tested biologically. Among these octapeptides, some contained N-terminal (Formula: see text) were 177 times and 113 times more potent, respectively, than somatostatin in test...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cai RZ,Szoke B,Lu R,Fu D,Redding TW,Schally AV

更新日期：1986-03-01 00:00:00

abstract：:The growth of 3T3 and SV101 3T3 cells in a lipid-depleted medium is enhanced by the addition of biotin or some fatty acids. The extent of enhancement depends on the fatty acid(s) supplied. The presence of linoleate is unique, since it induces a morphological alteration in 3T3 cells resulting in a cell similar to an SV...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Horwitz AF,Hatten ME,Burger MM

更新日期：1974-08-01 00:00:00

abstract：:Phenotypic alterations induced by the cytokinin 6-benzylaminopurine in cell suspensions of Nicotiana plumbaginifolia were studied at the level of the NH(2)-terminal sequence of the constituent proteins. Total protein extracts were separated by classical two-dimensional PAGE, and the proteins were recovered by electrob...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bauw G,De Loose M,Inzé D,Van Montagu M,Vandekerckhove J

更新日期：1987-07-01 00:00:00

abstract：:The SKW-3 cell line, which was established from the malignant cells of a patient with T-cell chronic lymphocytic leukemia, is characterized by a translocation involving chromosome 8 (band q24) and chromosome 14 (band q11) [t(8;14)(q24;q11)]. To determine the position of the gene encoding the alpha chain of the T-cell ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shima EA,Le Beau MM,McKeithan TW,Minowada J,Showe LC,Mak TW,Minden MD,Rowley JD,Diaz MO

更新日期：1986-05-01 00:00:00

abstract：:Mesothelin is a differentiation antigen present on the surface of ovarian cancers, mesotheliomas, and several other types of human cancers. Because among normal tissues, mesothelin is present only on mesothelial cells, it represents a good target for antibody-mediated delivery of cytotoxic agents. In the present study...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chowdhury PS,Viner JL,Beers R,Pastan I

更新日期：1998-01-20 00:00:00

abstract：:Here, we identified and characterized a Ly49 family member, designated as Ly49Q. The Ly49q gene encodes a 273-aa protein with an immunoreceptor tyrosine-based inhibitory motif (ITIM) at the N terminus of its cytoplasmic domain. We show that the ITIM of Ly49Q can recruit SHP-2 and SHP-1 in a tyrosine phosphorylation-de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Toyama-Sorimachi N,Tsujimura Y,Maruya M,Onoda A,Kubota T,Koyasu S,Inaba K,Karasuyama H

更新日期：2004-01-27 00:00:00

abstract：:The role of the serum proteolytic system plasminogen/plasminogen activator as a biochemical tool used by the glia or neurons, or both, for maintaining their temporary and flexible cellular interactions during histogenesis of the nervous system is under study. The present report identifies a glia cell type, the Schwann...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalderon N

更新日期：1984-11-01 00:00:00

abstract：:Down syndrome (DS) is the most common form of intellectual disability. The cognitive alterations in DS are thought to depend on brain regions critical for learning and memory such as the prefrontal cortex (PFC) and the hippocampus (HPC). Neuroimaging studies suggest that increased brain connectivity correlates with lo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alemany-González M,Gener T,Nebot P,Vilademunt M,Dierssen M,Puig MV

更新日期：2020-05-26 00:00:00

abstract：:Based on the hypothesis that features of the molecular program of normal wound healing might play an important role in cancer metastasis, we previously identified consistent features in the transcriptional response of normal fibroblasts to serum, and used this "wound-response signature" to reveal links between wound h...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang HY,Nuyten DS,Sneddon JB,Hastie T,Tibshirani R,Sørlie T,Dai H,He YD,van't Veer LJ,Bartelink H,van de Rijn M,Brown PO,van de Vijver MJ

更新日期：2005-03-08 00:00:00

abstract：:Light activates rod outer segment (ROS) phosphodiesterase (PDEase), as shown by previous biochemical and physiological studies. We have further investigated the role of PDEase in this system by injecting trypsin-activated PDEase, purified from bovine ROS, into single ROS of the isolated retina of the toad Bufo marinus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shimoda Y,Hurley JB,Miller WH

更新日期：1984-01-01 00:00:00

abstract：:Human fibroblast cells treated with a combination of inhibitors of protein and RNA synthesis [cycloheximide and actinomycin D as used to superinduce interferon beta (IFN-beta)] secrete two proteins with molecular masses of 22000 and 27000 kilodaltons (called 22-kDal and 27-kDal) that are precipitable with an antiserum...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Content J,De Wit L,Pierard D,Derynck R,De Clercq E,Fiers W

更新日期：1982-05-01 00:00:00

abstract：:Millions of individuals routinely remain awake for more than 18 h daily, which causes performance decrements. It is unknown if these functional impairments are the result of that extended wakefulness or from the associated shortened sleep durations. We therefore examined changes in objective reaction time performance ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McHill AW,Hull JT,Wang W,Czeisler CA,Klerman EB

更新日期：2018-06-05 00:00:00