Expression of cellular oncogenes in primary cells from human acute leukemias.

Abstract:

:The structure and the expression of 11 cellular oncogenes (protooncogenes) were analyzed in primary cells from 20 acute lymphocytic (ALL) and 31 acute myelogenous (AML) leukemia patients. Neoplastic cells, obtained prior to initiation of therapy, were purified and classified, on the basis of both surface antigen pattern and morphology, into pre-B, B, and T ALL and M1-M5 AML. RNA was extracted and analyzed for expression of cellular oncogenes coding for nuclear proteins (c-myc, c-myb, c-fos), the beta-chain of platelet-derived growth factor (c-sis), growth factor receptors or related proteins (c-src, c-abl, c-fes, c-erbB), or putative intermediate transducers of mitogenic signals (c-Ha-ras, c-Ki-ras, c-N-ras). Quantitative analysis of total RNA was carried out by dot blot hybridization to specific cDNA or genomic probes. Number and size of transcripts were evaluated by blot hybridization of electrophoretically fractionated poly(A)+ RNA. Expression of c-myc and c-myb was detected in all leukemic cells at variable levels and was characterized by well-defined patterns within ALL subtypes. Conversely, significant levels of c-fos transcripts were detected only in myelomonocytic (M4) and monocytic (M5) leukemias. Among the "src-family," c-fes was expressed more in AML than ALL, and c-abl was expressed at variable but not elevated levels in all leukemia types. c-Ha-ras was uniformly expressed at low levels, as in non-neoplastic cells. c-Ki-ras transcription was detected only in T ALL; N-ras expression was barely demonstrable. The structure of these protooncogenes was not grossly modified, as evaluated by Southern analysis, except for c-myc rearrangement in B ALL. These studies indicate that cellular oncogene expression in specific subtypes of leukemic cells may relate to either the proliferative activity (c-myc, c-myb) or the differentiation state (c-fos) of the cells, or possibly to expression of receptors for putative hemopoiesis-related growth factors (c-fes, c-abl). Our data provide a basis for in-depth analysis of protooncogene expression in normal and neoplastic hemopoiesis.

authors

Mavilio F,Sposi NM,Petrini M,Bottero L,Marinucci M,De Rossi G,Amadori S,Mandelli F,Peschle C

doi

10.1073/pnas.83.12.4394

subject

Has Abstract

pub_date

1986-06-01 00:00:00

pages

4394-8

issue

12

eissn

0027-8424

issn

1091-6490

journal_volume

83

pub_type

杂志文章
  • ATP-dependent bioluminescence in the firefly squid, Watasenia scintillans.

    abstract::The Japanese firefly squid, Watasenia scintillans, emits intense flashes of light from three tiny luminous organs that are located at the tip of each of a pair of ventral arms. Light is also produced from hundreds of other minute organs that are scattered over the body. The luminescence is due to an ATP-dependent reac...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.14.4629

    authors: Tsuji FI

    更新日期:1985-07-01 00:00:00

  • Somatocrinin receptor coupled with cAMP-dependent protein kinase on anterior pituitary granules.

    abstract::The molecular mechanism of growth hormone release by synthetic somatocrinin was investigated on purified hog anterior pituitary secretory granules; the granules were found to contain a cAMP-dependent protein kinase that catalyzed [gamma-32P]-ATP histone phosphorylation with maximal rates ranging from 1 to 5 nmol of Pi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.21.6538

    authors: Lewin MJ,Reyl-Desmars F,Ling N

    更新日期:1983-11-01 00:00:00

  • DNA bending and unbending by MutS govern mismatch recognition and specificity.

    abstract::DNA mismatch repair is central to the maintenance of genomic stability. It is initiated by the recognition of base-base mismatches and insertion/deletion loops by the family of MutS proteins. Subsequently, ATP induces a unique conformational change in the MutS-mismatch complex but not in the MutS-homoduplex complex th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2433654100

    authors: Wang H,Yang Y,Schofield MJ,Du C,Fridman Y,Lee SD,Larson ED,Drummond JT,Alani E,Hsieh P,Erie DA

    更新日期:2003-12-09 00:00:00

  • Structure-assisted redesign of a protein-zinc-binding site with femtomolar affinity.

    abstract::We have inserted a fourth protein ligand into the zinc coordination polyhedron of carbonic anhydrase II (CAII) that increases metal affinity 200-fold (Kd = 20 fM). The three-dimensional structures of threonine-199-->aspartate (T199D) and threonine-199-->glutamate (T199E) CAIIs, determined by x-ray crystallographic met...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.11.5017

    authors: Ippolito JA,Baird TT Jr,McGee SA,Christianson DW,Fierke CA

    更新日期:1995-05-23 00:00:00

  • Generating sparse and selective third-order responses in the olfactory system of the fly.

    abstract::In the antennal lobe of Drosophila, information about odors is transferred from olfactory receptor neurons (ORNs) to projection neurons (PNs), which then send axons to neurons in the lateral horn of the protocerebrum (LHNs) and to Kenyon cells (KCs) in the mushroom body. The transformation from ORN to PN responses can...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1005635107

    authors: Luo SX,Axel R,Abbott LF

    更新日期:2010-06-08 00:00:00

  • IL-2 negatively regulates IL-7 receptor alpha chain expression in activated T lymphocytes.

    abstract::Interleukin (IL)-2 is a type I four-alpha-helical bundle cytokine that plays vital roles in antigen-mediated proliferation of peripheral blood T cells and also is critical for activation-induced cell death. We now demonstrate that IL-2 potently decreases expression of IL-7 receptor alpha chain (IL-7Ralpha) mRNA and pr...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.212214999

    authors: Xue HH,Kovanen PE,Pise-Masison CA,Berg M,Radovich MF,Brady JN,Leonard WJ

    更新日期:2002-10-15 00:00:00

  • High levels of nuclear heat-shock factor 1 (HSF1) are associated with poor prognosis in breast cancer.

    abstract::Heat-shock factor 1 (HSF1) is the master transcriptional regulator of the cellular response to heat and a wide variety of other stressors. We previously reported that HSF1 promotes the survival and proliferation of malignant cells. At this time, however, the clinical and prognostic significance of HSF1 in cancer is un...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1115031108

    authors: Santagata S,Hu R,Lin NU,Mendillo ML,Collins LC,Hankinson SE,Schnitt SJ,Whitesell L,Tamimi RM,Lindquist S,Ince TA

    更新日期:2011-11-08 00:00:00

  • Structural flexibility at a major conserved antibody target on hepatitis C virus E2 antigen.

    abstract::Hepatitis C virus (HCV) is a major cause of liver disease, affecting over 2% of the world's population. The HCV envelope glycoproteins E1 and E2 mediate viral entry, with E2 being the main target of neutralizing antibody responses. Structural investigations of E2 have produced templates for vaccine design, including t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1609780113

    authors: Kong L,Lee DE,Kadam RU,Liu T,Giang E,Nieusma T,Garces F,Tzarum N,Woods VL Jr,Ward AB,Li S,Wilson IA,Law M

    更新日期:2016-11-08 00:00:00

  • Contraction characteristics and ATPase activity of skeletal muscle fibers in the presence of antibody to myosin subfragment 2.

    abstract::To investigate the role of the myosin hinge region in muscle contraction, we examined the contraction characteristics and Mg-ATPase activity of glycerinated muscle fibers prepared from rabbit psoas in the presence and absence of polyclonal antibody directed against the subfragment 2 (S-2) region of myosin. The antibod...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.13.6134

    authors: Sugi H,Kobayashi T,Gross T,Noguchi K,Karr T,Harrington WF

    更新日期:1992-07-01 00:00:00

  • Serum proteome profiling detects myelodysplastic syndromes and identifies CXC chemokine ligands 4 and 7 as markers for advanced disease.

    abstract::Myelodysplastic syndromes (MDS) are among the most frequent hematologic malignancies. Patients have a short survival and often progress to acute myeloid leukemia. The diagnosis of MDS can be difficult; there is a paucity of molecular markers, and the pathophysiology is largely unknown. Therefore, we conducted a multic...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章,多中心研究

    doi:10.1073/pnas.0610330104

    authors: Aivado M,Spentzos D,Germing U,Alterovitz G,Meng XY,Grall F,Giagounidis AA,Klement G,Steidl U,Otu HH,Czibere A,Prall WC,Iking-Konert C,Shayne M,Ramoni MF,Gattermann N,Haas R,Mitsiades CS,Fung ET,Libermann TA

    更新日期:2007-01-23 00:00:00

  • Label-free imaging, detection, and mass measurement of single viruses by surface plasmon resonance.

    abstract::We report on label-free imaging, detection, and mass/size measurement of single viral particles in solution by high-resolution surface plasmon resonance microscopy. Diffraction of propagating plasmon waves along a metal surface by the viral particles creates images of the individual particles, which allow us to detect...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1005264107

    authors: Wang S,Shan X,Patel U,Huang X,Lu J,Li J,Tao N

    更新日期:2010-09-14 00:00:00

  • Anthropogenic strath terrace formation caused by reduced sediment retention.

    abstract::Across North America, human activities have been shown to cause river incision into unconsolidated alluvium. Human-caused erosion through bedrock, however, has only been observed in local and isolated outcrops. Here, we test whether splash-dam logging, which decreased in-stream alluvial cover by removing much of the a...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1814627116

    authors: Schanz SA,Montgomery DR,Collins BD

    更新日期:2019-04-30 00:00:00

  • Oligouridylate stretches in heterogeneous nuclear RNA.

    abstract::Three classes of heterogeneous nuclear RNA (HnRNA) alpha, beta, and gamma, with different chemical and physical properties, can be identified in the early sea urchin embryo by hybridization with poly(U). The relative amounts of these classes vary as a function of embryonic development. It is demonstrated here that the...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.74.6.2217

    authors: Dubroff LM

    更新日期:1977-06-01 00:00:00

  • Fate of the replisome following arrest by UV-induced DNA damage in Escherichia coli.

    abstract::Accurate replication in the presence of DNA damage is essential to genome stability and viability in all cells. In Escherichia coli, DNA replication forks blocked by UV-induced damage undergo a partial resection and RecF-catalyzed regression before synthesis resumes. These processing events generate distinct structura...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1300624110

    authors: Jeiranian HA,Schalow BJ,Courcelle CT,Courcelle J

    更新日期:2013-07-09 00:00:00

  • Toward high-energy-density, high-efficiency, and moderate-temperature chip-scale thermophotovoltaics.

    abstract::The challenging problem of ultra-high-energy-density, high-efficiency, and small-scale portable power generation is addressed here using a distinctive thermophotovoltaic energy conversion mechanism and chip-based system design, which we name the microthermophotovoltaic (μTPV) generator. The approach is predicted to be...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1301004110

    authors: Chan WR,Bermel P,Pilawa-Podgurski RC,Marton CH,Jensen KF,Senkevich JJ,Joannopoulos JD,Soljacic M,Celanovic I

    更新日期:2013-04-02 00:00:00

  • Stability of local rings of dimension 2.

    abstract::The notion of stability of a local ring arises naturally in investigating completed moduli spaces via geometric invariant theory. A scheme corresponding to a boundary point of a moduli space of nonsingular varieties cannot have unstable singular points. This paper reports some results concerning the stability of surfa...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.75.9.4085

    authors: Shah J

    更新日期:1978-09-01 00:00:00

  • Protein-solvent preferential interactions, protein hydration, and the modulation of biochemical reactions by solvent components.

    abstract::Solvent additives (cosolvents, osmolytes) modulate biochemical reactions if, during the course of the reaction, there is a change in preferential interactions of solvent components with the reacting system. Preferential interactions can be expressed in terms of preferential binding of the cosolvent or its preferential...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.122225399

    authors: Timasheff SN

    更新日期:2002-07-23 00:00:00

  • Functional relevance of the histone gammaH2Ax in the response to DNA damaging agents.

    abstract::The phosphorylation of H2Ax on its S139 site, γH2Ax, is important during DNA double-strand repair and is considered necessary for assembly of repair complexes, but its functional role after other kinds of DNA damage is less clear. We have measured the survival of isogenic mouse cell lines with the H2Ax gene knocked ou...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1105866108

    authors: Revet I,Feeney L,Bruguera S,Wilson W,Dong TK,Oh DH,Dankort D,Cleaver JE

    更新日期:2011-05-24 00:00:00

  • An empirical basis for Mach bands.

    abstract::Mach bands, the illusory brightness maxima and minima perceived at the initiation and termination of luminance gradients, respectively, are generally considered a direct perceptual manifestation of lateral inhibitory interactions among retinal or other lower order visual neurons. Here we examine an alternative explana...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.9.5239

    authors: Lotto RB,Williams SM,Purves D

    更新日期:1999-04-27 00:00:00

  • Transcription of the polyoma virus genome: synthesis and cleavage of giant late polyoma-specific RNA.

    abstract::The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized "late" polyoma-specific RNA appears as "giant" molecules of heterogeneous size, up to several times large...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.68.9.2231

    authors: Acheson NH,Buetti E,Scherrer K,Weil R

    更新日期:1971-09-01 00:00:00

  • Evidence for a significant urbanization effect on climate in China.

    abstract::China has experienced rapid urbanization and dramatic economic growth since its reform process started in late 1978. In this article, we present evidence for a significant urbanization effect on climate based on analysis of impacts of land-use changes on surface temperature in southeast China, where rapid urbanization...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0400357101

    authors: Zhou L,Dickinson RE,Tian Y,Fang J,Li Q,Kaufmann RK,Tucker CJ,Myneni RB

    更新日期:2004-06-29 00:00:00

  • The cytoplasmic component of the bacterial flagellar motor.

    abstract::We have used electron microscopy to examine freshly isolated Salmonella typhimurium and Escherichia coli basal flagellar fragments, purified without resort to extremes of pH or ionic strength. Such fragments contain the large bell-like basal structures visualized recently in freeze-substituted or fixed preparations. W...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.13.5956

    authors: Khan IH,Reese TS,Khan S

    更新日期:1992-07-01 00:00:00

  • A randomized controlled design reveals barriers to citizenship for low-income immigrants.

    abstract::Citizenship endows legal protections and is associated with economic and social gains for immigrants and their communities. In the United States, however, naturalization rates are relatively low. Yet we lack reliable knowledge as to what constrains immigrants from applying. Drawing on data from a public/private natura...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1714254115

    authors: Hainmueller J,Lawrence D,Gest J,Hotard M,Koslowski R,Laitin DD

    更新日期:2018-01-30 00:00:00

  • DNA hybridization evidence for the Australasian affinity of the American marsupial Dromiciops australis.

    abstract::DNA hybridization was used to compare representatives of the major groups of marsupials and a eutherian outgroup. Because of the large genetic distances separating marsupial families, trees were calculated from normalized percentages of hybridization; thermal-melting statistics, however, gave identical topologies for ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.23.10465

    authors: Kirsch JA,Dickerman AW,Reig OA,Springer MS

    更新日期:1991-12-01 00:00:00

  • RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection.

    abstract::AIDS remains incurable due to the permanent integration of HIV-1 into the host genome, imparting risk of viral reactivation even after antiretroviral therapy. New strategies are needed to ablate the viral genome from latently infected cells, because current methods are too inefficient and prone to adverse off-target e...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1405186111

    authors: Hu W,Kaminski R,Yang F,Zhang Y,Cosentino L,Li F,Luo B,Alvarez-Carbonell D,Garcia-Mesa Y,Karn J,Mo X,Khalili K

    更新日期:2014-08-05 00:00:00

  • Use of Monte Carlo calculations in the study of microtubule subunit kinetics.

    abstract::GTP-tubulin forms a cap on microtubule ends during aggregation. The bulk of the microtubule is GDP-tubulin. This complicates the usual simple kinetic theory of subunit exchange at microtubule ends to such an extent that Monte Carlo calculations are needed to handle the complications, except in special cases. The Monte...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.80.24.7520

    authors: Chen Y,Hill TL

    更新日期:1983-12-01 00:00:00

  • Direct high-resolution mapping of electrocatalytic activity of semi-two-dimensional catalysts with single-edge sensitivity.

    abstract::The catalytic activity of low-dimensional electrocatalysts is highly dependent on their local atomic structures, particularly those less-coordinated sites found at edges and corners; therefore, a direct probe of the electrocatalytic current at specified local sites with true nanoscopic resolution has become critically...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1821091116

    authors: Sun T,Wang D,Mirkin MV,Cheng H,Zheng JC,Richards RM,Lin F,Xin HL

    更新日期:2019-06-11 00:00:00

  • Sticky ions in biological systems.

    abstract::Aqueous gel sieving chromatography on Sephadex G-10 of the Group IA cations (Li+, Na+, K+, Rb+, Cs+) plus NH4+ as the Cl- salts, in combination with previous results for the halide anions (F-, Cl-, Br-, I-) as the Na+ salts [Washabaugh, M.W. & Collins, K.D. (1986) J. Biol. Chem. 261, 12477-12485], leads to the followi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.12.5553

    authors: Collins KD

    更新日期:1995-06-06 00:00:00

  • Isolation and expression of rat liver sepiapterin reductase cDNA.

    abstract::Sepiapterin reductase (7,8-dihydrobiopterin: NADP+ oxidoreductase, EC 1.1.1.153) catalyzes the terminal step in the biosynthetic pathway for tetrahydrobiopterin, the cofactor necessary for aromatic amino acid hydroxylation. We report here the isolation of a cDNA clone for rat liver sepiapterin reductase. The cDNA has ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.16.6436

    authors: Citron BA,Milstien S,Gutierrez JC,Levine RA,Yanak BL,Kaufman S

    更新日期:1990-08-01 00:00:00

  • Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1.

    abstract::Transcription, splicing, and translation are potentially coordinately regulatable in a temporospatial-dependent manner, although supporting experimental evidence for this notion is scarce. Yeast two-hybrid screening of a mammary gland cDNA library with human p21-activated kinase 1 (Pak1) as bait identified polyC-RNA-b...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0701065104

    authors: Meng Q,Rayala SK,Gururaj AE,Talukder AH,O'Malley BW,Kumar R

    更新日期:2007-04-03 00:00:00