Casein kinase II promotes target silencing by miRISC through direct phosphorylation of the DEAD-box RNA helicase CGH-1.

abstract：:The amino-terminal amino acid sequence and several internal peptide sequences of angiotensin I-converting enzyme (ACE; peptidyl-dipeptidase A, kininase II; EC 3.4.15.1) purified from human kidney were used to design oligonucleotide probes. The nucleotide sequence of ACE mRNA was determined by molecular cloning of the ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Soubrier F,Alhenc-Gelas F,Hubert C,Allegrini J,John M,Tregear G,Corvol P

更新日期：1988-12-01 00:00:00

abstract：:Membranes were purified from rat muscle by a differential centrifugation method that avoids the use of salt extraction or incubations at elevated temperature. Three populations of membrane-limited vesicles were defined having average densities of 1.112 (fraction I), 1.141 (fraction II), and 1.158 (fraction III) g/ml i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barchi RL,Bonilla E,Wong M

更新日期：1977-01-01 00:00:00

abstract：:The receptor 2B4 belongs to the Ig superfamily and is found on the surface of all murine natural killer (NK) cells as well as T cells displaying non-MHC-restricted cytotoxicity. Previous studies have suggested that 2B4 is an activating molecule because cross-linking of this receptor results in increased cytotoxicity a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schatzle JD,Sheu S,Stepp SE,Mathew PA,Bennett M,Kumar V

更新日期：1999-03-30 00:00:00

abstract：:Prototrophic hybrids formed from an adenine-requiring Chinese hamster cell and human fibroblasts uniformly display new esterase activity that differs from that of either parental cell in electrophoretic mobility and substrate specificity. The hybrids that grew in the selective medium and possessed the new esterase act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kao FT,Puck TT

更新日期：1972-11-01 00:00:00

abstract：:We have used a membrane-leak mass spectrometer to follow the time courses of H2 evolution and substrate reduction by nitrogenase [reduced ferredoxin:dinitrogen oxidoreductase (ATP-hydrolyzing), EC 1.18.6.1]. In the absence of added substrates, dinitrogenase passes all of its electrons to protons to form H2, but when a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liang J,Burris RH

更新日期：1988-12-01 00:00:00

abstract：:Current theory indicates that mitochondria were obtained 1.5 billion years ago from an ancient prokaryote. The mitochondria provided the capacity for aerobic respiration, the creation of the eukaryotic cell, and eventually complex multicellular organisms. Recent reports have found that mitochondria play essential role...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Spees JL,Olson SD,Whitney MJ,Prockop DJ

更新日期：2006-01-31 00:00:00

abstract：:We have isolated and determined the sequence of a genomic clone containing the gene for a human class I transplantation antigen. The gene contains seven exons. The first five exons code respectively for a signal peptide, for the first, second, and third extracellular domains of the protein molecule, and for the transm...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Malissen M,Malissen B,Jordan BR

更新日期：1982-02-01 00:00:00

abstract：:Tumor-specific molecules are needed across diverse areas of oncology for use in early detection, diagnosis, prognosis and therapy. Large and growing public databases of transcriptome sequencing data (RNA-seq) derived from tumors and normal tissues hold the potential of yielding tumor-specific molecules, but because th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barrett CL,DeBoever C,Jepsen K,Saenz CC,Carson DA,Frazer KA

更新日期：2015-06-09 00:00:00

abstract：:Animal mitochondrial DNA (mtDNA) maintains a displacement loop (D loop) at the heavy strand origin of replication. These D loops represent sharply limited synthesis of heavy strands and provide a unique opportunity to examine the termination of DNA synthesis. Direct sizing at the nucleotide level indicates that the 3'...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Doda JN,Wright CT,Clayton DA

更新日期：1981-10-01 00:00:00

abstract：:The gene networks underlying closure of the optic fissure during vertebrate eye development are poorly understood. Here, we profile global gene expression during optic fissure closure using laser capture microdissected (LCM) tissue from the margins of the fissure. From these data, we identify a unique role for the C(2...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown JD,Dutta S,Bharti K,Bonner RF,Munson PJ,Dawid IB,Akhtar AL,Onojafe IF,Alur RP,Gross JM,Hejtmancik JF,Jiao X,Chan WY,Brooks BP

更新日期：2009-02-03 00:00:00

abstract：:A spectroscopic interpretation of incoherent neutron scattering experiments is presented which is based on Franck-Condon-type probabilities for scattering-induced transitions between quantum states of the target. The resulting expressions for the scattering functions enable an energy landscape-oriented analysis of neu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kneller GR

更新日期：2018-09-18 00:00:00

abstract：:The formation of viable genetic chimeras in mammals through the transfer of cells between siblings in utero is rare. Using microsatellite DNA markers, we show here that chimerism in marmoset (Callithrix kuhlii) twins is not limited to blood-derived hematopoietic tissues as was previously described. All somatic tissue ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ross CN,French JA,Ortí G

更新日期：2007-04-10 00:00:00

abstract：:Variants of isovaleryl-CoA dehydrogenase (IVDHase, EC 1.3.99.10) in 15 isovaleric acidemia fibroblast lines were analyzed using [35S]methionine labeling, immunoprecipitation with anti-rat IVDHase antiserum, and NaDodSo4/polyacrylamide gel electrophoresis. Five distinct variants of IVDHase were detected. The molecular ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ikeda Y,Keese SM,Tanaka K

更新日期：1985-10-01 00:00:00

abstract：:We have isolated lentivirus strains that are related to the human immunodeficiency virus (HIV) from African green monkeys (Cercopithecus aethiops; AGM). Although immunologically related, these SIVagm are clearly distinct from other simian immunodeficiency virus (SIV) isolates, including isolates from Macaca mulatta (S...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kraus G,Werner A,Baier M,Binniger D,Ferdinand FJ,Norley S,Kurth R

更新日期：1989-04-01 00:00:00

abstract：:By using differential display PCR, we have identified 98 cDNA fragments from the rat dorsal hippocampus that are expressed differentially between the fast learners and slow learners in the water maze learning task. One of these cDNA fragments encodes the rat serum- and glucocorticoid-inducible kinase (sgk) gene. North...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tsai KJ,Chen SK,Ma YL,Hsu WL,Lee EH

更新日期：2002-03-19 00:00:00

abstract：:Eye blinking is one of the most frequent human actions. The control of blinking is thought to reflect complex interactions between maintaining clear and healthy vision and influences tied to central dopaminergic functions including cognitive states, psychological factors, and medical conditions. The most imminent cons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoppe D,Helfmann S,Rothkopf CA

更新日期：2018-02-27 00:00:00

abstract：:Cytochrome c oxidase (COX) is a 13-subunit protein complex that catalyzes the last step in mitochondrial electron transfer in mammals. Of the 10 subunits encoded by nuclear DNA (three are mtDNA products), some are expressed as tissue- and/or development-specific isoforms. For COX subunit VIII, previous work showed tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goldberg A,Wildman DE,Schmidt TR,Huttemann M,Goodman M,Weiss ML,Grossman LI

更新日期：2003-05-13 00:00:00

abstract：:Receptor-mediated endocytosis proceeds by transfer of receptor-ligand complexes from clathrin-coated pits at the cell surface to uncoated endocytic vesicles termed receptosomes (or endosomes). These vesicles have now been purified more than 37-fold based on their content of newly internalized epidermal growth factor. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dickson RB,Beguinot L,Hanover JA,Richert ND,Willingham MC,Pastan I

更新日期：1983-09-01 00:00:00

abstract：:Acquired resistance against antimalarial drugs has further increased the need for an effective malaria vaccine. The current leading candidate, RTS,S, is a recombinant circumsporozoite protein (CSP)-based vaccine against Plasmodium falciparum that contains 19 NANP repeats followed by a thrombospondin repeat domain. Alt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oyen D,Torres JL,Wille-Reece U,Ockenhouse CF,Emerling D,Glanville J,Volkmuth W,Flores-Garcia Y,Zavala F,Ward AB,King CR,Wilson IA

更新日期：2017-11-28 00:00:00

abstract：:Neuronal hyperexcitability in both injured and adjacent uninjured neurons is associated with states of chronic injury and pain and is likely subject to neuroinflammatory processes. Chronic inflammatory responses are largely orchestrated by chemokines. One chemokine, monocyte chemoattractant protein-1 (MCP-1), in the p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： White FA,Sun J,Waters SM,Ma C,Ren D,Ripsch M,Steflik J,Cortright DN,Lamotte RH,Miller RJ

更新日期：2005-09-27 00:00:00

abstract：:The endometrial epithelium of the uterus regenerates periodically. The cellular source of newly regenerated endometrial epithelia during a mouse estrous cycle or a human menstrual cycle is presently unknown. Here, I have used single-cell lineage tracing in the whole mouse uterus to demonstrate that epithelial stem cel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jin S

更新日期：2019-04-02 00:00:00

abstract：:Previous work shows that the adolescent reward system is hyperactive, but this finding may be confounded by differences in how teens value money. To address this, we examined the neural ontogeny of objective value representation. Adolescent and adult participants performed a monetary gambling task in which they chose ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barkley-Levenson E,Galván A

更新日期：2014-01-28 00:00:00

abstract：:The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion. We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bruce WB,Christensen AH,Klein T,Fromm M,Quail PH

更新日期：1989-12-01 00:00:00

abstract：:N6-methyladenosine (m6A) represents one of the most common RNA modifications in eukaryotes. Specific m6A writer, eraser, and reader proteins have been identified. As an m6A eraser, ALKBH5 specifically removes m6A from target mRNAs and inactivation of Alkbh5 leads to male infertility in mice. However, the underlying mo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tang C,Klukovich R,Peng H,Wang Z,Yu T,Zhang Y,Zheng H,Klungland A,Yan W

更新日期：2018-01-09 00:00:00

abstract：:Deleting an important active-site residue of diphtheria toxin, glutamic acid-148, reduces the toxin's ADP-ribosyltransferase activity by a factor of greater than 10(4). We considered using this mutation to construct a recombinant toxoid for expression by live attenuated vaccines and explored second-site mutations that...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Killeen KP,Escuyer V,Mekalanos JJ,Collier RJ

更新日期：1992-07-01 00:00:00

abstract：:The release of urine, or micturition, serves a fundamental physiological function and, in many species, is critical for social communication. In mice, the pattern of urine release is modulated by external and internal factors and transmitted to the spinal cord via the pontine micturition center (PMC). Here, we exploit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hyun M,Taranda J,Radeljic G,Miner L,Wang W,Ochandarena N,Huang KW,Osten P,Sabatini BL

更新日期：2021-01-05 00:00:00

abstract：:A remarkable property of Ca2+ fluxes through the inositol 1,4,5-trisphosphate (InsP3)-gated Ca2+ channel is that successive increments of InsP3 induce repeated transient release of accumulated Ca2+. The initial aim of this study was to propose a model, based on hypotheses compatible with the current description of thi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swillens S,Combettes L,Champeil P

更新日期：1994-10-11 00:00:00

abstract：:The effect of pH on the membrane-fusion activity of Sendai virus was examined (pH 5.0-9.5) by using, as assays of activity, hemolysis of chicken erythrocytes and the fusion of baby hamster kidney (BHK-21) cells. Exposure of virus to basic pH increased fusion activity; the optimum pH was found to be approximately equal...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hsu MC,Scheid A,Choppin PW

更新日期：1982-10-01 00:00:00

abstract：:Pith parenchyma cells store water in various plant organs. These cells are especially important for producing sugar and ethanol from the sugar juice of grass stems. In many plants, the death of pith parenchyma cells reduces their stem water content. Previous studies proposed that a hypothetical D gene might be respons...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fujimoto M,Sazuka T,Oda Y,Kawahigashi H,Wu J,Takanashi H,Ohnishi T,Yoneda JI,Ishimori M,Kajiya-Kanegae H,Hibara KI,Ishizuna F,Ebine K,Ueda T,Tokunaga T,Iwata H,Matsumoto T,Kasuga S,Yonemaru JI,Tsutsumi N

更新日期：2018-09-11 00:00:00

abstract：:T cell-mediated autoimmune diseases such as type 1 diabetes (T1D) are believed to be the result in part of inefficient negative selection of self-specific thymocytes. However, the events regulating thymic negative selection are not fully understood. In the current study, we demonstrate that nonobese diabetic (NOD) mic...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wallet MA,Flores RR,Wang Y,Yi Z,Kroger CJ,Mathews CE,Earp HS,Matsushima G,Wang B,Tisch R

更新日期：2009-03-24 00:00:00