Abstract:
:Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 micrograms/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from human plasma. These studies provide evidence that gamma-carboxylation can occur at high levels in the mammary gland of a pig.
journal_name
Proc Natl Acad Sci U S Aauthors
Velander WH,Johnson JL,Page RL,Russell CG,Subramanian A,Wilkins TD,Gwazdauskas FC,Pittius C,Drohan WNdoi
10.1073/pnas.89.24.12003keywords:
subject
Has Abstractpub_date
1992-12-15 00:00:00pages
12003-7issue
24eissn
0027-8424issn
1091-6490journal_volume
89pub_type
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