Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers.

abstract：:The rigidity of numerous biological filaments and crafted microrods has been conveniently deduced from the analysis of their thermal fluctuations. However, the difficulty of measuring nanometric displacements with an optical microscope has so far limited such studies to sufficiently flexible rods, of which the persist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gerbal F,Wang Y

更新日期：2017-03-07 00:00:00

abstract：:Duchenne muscular dystrophy (DMD) is a genetic disorder caused by loss of the protein dystrophin. In humans, DMD has early onset, causes developmental delays, muscle necrosis, loss of ambulation, and death. Current animal models have been challenged by their inability to model the early onset and severity of the disea...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hughes KJ,Rodriguez A,Flatt KM,Ray S,Schuler A,Rodemoyer B,Veerappan V,Cuciarone K,Kullman A,Lim C,Gutta N,Vemuri S,Andriulis V,Niswonger D,Barickman L,Stein W,Singhvi A,Schroeder NE,Vidal-Gadea AG

更新日期：2019-02-26 00:00:00

abstract：:Lethal factor, the principal virulence factor of Bacillus anthracis, inhibits mitogen-activated protein kinase (MAPK) signaling by proteolytically cleaving MAPK kinases. Edema factor, another component of anthrax toxin, is an adenylate cyclase, which increases intracellular cAMP. Inhibition of MAPK signaling with eith...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Koo HM,VanBrocklin M,McWilliams MJ,Leppla SH,Duesbery NS,Vande Woude GF

更新日期：2002-03-05 00:00:00

abstract：:Single molecule fluorescence polarization techniques have been used for three-dimensional (3D) orientation measurements to observe the dynamic properties of single molecules. However, only few techniques can simultaneously measure 3D orientation and position. Furthermore, these techniques often require complex equipme...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohmachi M,Komori Y,Iwane AH,Fujii F,Jin T,Yanagida T

更新日期：2012-04-03 00:00:00

abstract：:The enzyme-AMP reaction intermediate of the 102-kDa bovine DNA ligase I was digested with trypsin, and the adenylylated peptide was isolated by chromatography under conditions that maintain the acid-labile phosphoramidate bond. Microsequencing of the peptide showed that it contains an internal trypsin-resistant lysine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tomkinson AE,Totty NF,Ginsburg M,Lindahl T

更新日期：1991-01-15 00:00:00

abstract：:Phenylglyoxal is an arginine-specific reagent that inactivates creatine kinase (CK). Previous results suggest that modification of the dimeric enzyme at a single arginine residue per subunit causes complete inactivation accompanied by the loss of nucleotide binding; the actual site of modification was not identified. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wood TD,Guan Z,Borders CL Jr,Chen LH,Kenyon GL,McLafferty FW

更新日期：1998-03-31 00:00:00

abstract：:The specificity of human skin collagenase and of an enzyme from an invasive tumor were studied by using types I, II, III, IV, and V (AB) collagen as substrates. Human skin collagenase degraded types I, II, and III collagen, producing the characteristic 3/4 and 1/4 cleavage products, but failed to degrade type IV or V ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liotta LA,Abe S,Robey PG,Martin GR

更新日期：1979-05-01 00:00:00

abstract：:Despite high structural homology between NO reductases (NORs) and heme-copper oxidases (HCOs), factors governing their reaction specificity remain to be understood. Using a myoglobin-based model of NOR (FeBMb) and tuning its heme redox potentials (E°') to cover the native NOR range, through manipulating hydrogen bondi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bhagi-Damodaran A,Reed JH,Zhu Q,Shi Y,Hosseinzadeh P,Sandoval BA,Harnden KA,Wang S,Sponholtz MR,Mirts EN,Dwaraknath S,Zhang Y,Moënne-Loccoz P,Lu Y

更新日期：2018-06-12 00:00:00

abstract：:While we have come to appreciate the architectural complexity of microbially synthesized secondary metabolites, far less attention has been paid to linking their structural features with possible modes of action. This is certainly the case with tropodithietic acid (TDA), a broad-spectrum antibiotic generated by marine...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wilson MZ,Wang R,Gitai Z,Seyedsayamdost MR

更新日期：2016-02-09 00:00:00

abstract：:The Bacillus subtilis phage SP01, whose DNA contains 5-hydroxymethyluracil (hmUra) in place of thymine, codes for an abundant, small, basic protein called TF1. TF1 binds preferentially to hydroxymethyluracil-containing DNA and thereby selectively inhibits transcription of such DNA in vitro. The gene for TF1 has been s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Greene JR,Brennan SM,Andrew DJ,Thompson CC,Richards SH,Heinrikson RL,Geiduschek EP

更新日期：1984-11-01 00:00:00

abstract：:The primate visual motion system performs numerous functions essential for survival in a dynamic visual world. Prominent among these functions is the ability to recover and represent the trajectories of objects in a form that facilitates behavioral responses to those movements. The first step toward this goal, which c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Albright TD,Stoner GR

更新日期：1995-03-28 00:00:00

abstract：:Rapid and efficient protocols to generate oligodendrocytes (OL) from human induced pluripotent stem cells (iPSC) are currently lacking, but may be a key technology to understand the biology of myelin diseases and to develop treatments for such disorders. Here, we demonstrate that the induction of three transcription f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ehrlich M,Mozafari S,Glatza M,Starost L,Velychko S,Hallmann AL,Cui QL,Schambach A,Kim KP,Bachelin C,Marteyn A,Hargus G,Johnson RM,Antel J,Sterneckert J,Zaehres H,Schöler HR,Baron-Van Evercooren A,Kuhlmann T

更新日期：2017-03-14 00:00:00

abstract：:Model compositions of Earth, Venus, and Mercury are calculated from the premise that planets and chondrites underwent four identical fractionation processes in the solar nebula. Because elements of similar properties stay together in these processes, five constraints suffice to define the composition of a planet: mass...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Morgan JW,Anders E

更新日期：1980-12-01 00:00:00

abstract：:The influx of Ca ions into the presynaptic nerve terminal through ion channels is a key link between the action potential and the release of chemical transmitters. It is not clear, however, which types of Ca channel are involved in neurosecretion at vertebrate synapses. In particular, there is disagreement as to wheth...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stanley EF,Atrakchi AH

更新日期：1990-12-01 00:00:00

abstract：:(3)H-actinomycin D, a guanine-binding agent, labels fixed human chromosomes nonrandomly. Actinomycin D added in G2 inhibits secondary constrictions and breaks chromosomes. There is some tendency for label to be concentrated at the ends of chromosomes and near the centromere. Labeling with (3)H-thymidine in the late st...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miles CP

更新日期：1970-03-01 00:00:00

abstract：:The fluxes of arginine and citrulline through plasma and the rate of conversion of labeled citrulline to arginine were estimated in two pilot studies (with a total of six adult subjects) and in a dietary study with five healthy young men. These latter subjects received an L-amino acid-based diet that was arginine-rich...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castillo L,Chapman TE,Sanchez M,Yu YM,Burke JF,Ajami AM,Vogt J,Young VR

更新日期：1993-08-15 00:00:00

abstract：:In previous studies we showed that 5 days of treatment with granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) mobilized murine repopulating cells to the peripheral blood (PB) and that these cells could be efficiently transduced with retroviral vectors. We also found that, 7-14 days after cytokin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dunbar CE,Seidel NE,Doren S,Sellers S,Cline AP,Metzger ME,Agricola BA,Donahue RE,Bodine DM

更新日期：1996-10-15 00:00:00

abstract：:Intrinsically disordered proteins (IDPs) adopt heterogeneous ensembles of conformations under physiological conditions. Understanding the relationship between amino acid sequence and conformational ensembles of IDPs can help clarify the role of disorder in physiological function. Recent studies revealed that polar IDP...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mao AH,Crick SL,Vitalis A,Chicoine CL,Pappu RV

更新日期：2010-05-04 00:00:00

abstract：:The accumulation of misfolded proteins in the endoplasmic reticulum (ER) causes ER stress and triggers the unfolded protein response (UPR). Failure to resolve ER stress leads to apoptotic cell death via a yet unclear mechanism. Here, we show that RNF183, a membrane-spanning RING finger protein, localizes to the ER and...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu Y,Li X,Jia J,Zhang Y,Li J,Zhu Z,Wang H,Tang J,Hu J

更新日期：2018-03-20 00:00:00

abstract：:Radiation dose rates were evaluated in three areas neighboring a restricted area within a 20- to 50-km radius of the Fukushima Daiichi Nuclear Power Plant in August-September 2012 and projected to 2022 and 2062. Study participants wore personal dosimeters measuring external dose equivalents, almost entirely from depos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harada KH,Niisoe T,Imanaka M,Takahashi T,Amako K,Fujii Y,Kanameishi M,Ohse K,Nakai Y,Nishikawa T,Saito Y,Sakamoto H,Ueyama K,Hisaki K,Ohara E,Inoue T,Yamamoto K,Matsuoka Y,Ohata H,Toshima K,Okada A,Sato H,Kuwa

更新日期：2014-03-11 00:00:00

abstract：:Unit-resolution mass spectra have been obtained for peptides as large as 17 kDa, providing information on impurities and adduct ions, as well as accurate molecular weight values. Electrospray ionization produces many multiply-charged species of the same mass; isotopic peak resolution provides direct charge state assig...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loo JA,Quinn JP,Ryu SI,Henry KD,Senko MW,McLafferty FW

更新日期：1992-01-01 00:00:00

abstract：:Alternaria solani, the causal agent of early blight disease in potato, produces two host-specific, lipidlike toxins in culture. Both compounds are required in the leaf bioassay for the elicitation of typical early blight symptoms, but the compounds are individually inactive. The procedures for the preparation of both ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matern U,Strobel G,Shepard J

更新日期：1978-10-01 00:00:00

abstract：:We have investigated the regulation of the expression of two growth factors found in vascular smooth muscle, transforming growth factor alpha (TGF alpha) and basic fibroblast growth factor (bFGF). Cells cultured in medium containing 30 mM glucose exhibited a 2-fold increase in TGF alpha mRNA and a 3-fold increase in b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McClain DA,Paterson AJ,Roos MD,Wei X,Kudlow JE

更新日期：1992-09-01 00:00:00

abstract：:Acid-sensing ion channels (ASICs) are trimeric cation-selective ion channels activated by protons in the physiological range. Recent reports have revealed that postsynaptically localized ASICs contribute to the excitatory postsynaptic current by responding to the transient acidification of the synaptic cleft that acco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： MacLean DM,Jayaraman V

更新日期：2017-03-21 00:00:00

abstract：:Proteins synthesized in the cell can begin to fold during translation before the entire polypeptide has been produced, which may be particularly relevant to the folding of multidomain proteins. Here, we study the cotranslational folding of adjacent domains from the cytoskeletal protein α-spectrin using force profile a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kemp G,Nilsson OB,Tian P,Best RB,von Heijne G

更新日期：2020-06-23 00:00:00

abstract：:The nematode Caenorhabditis elegans exhibits behavioral responses to many volatile odorants. Chemotaxis toward one such odorant, diacetyl (butanedione), requires the function of a seven-transmembrane receptor protein encoded by the odr-10 gene. To determine directly whether ODR-10 protein is an odorant receptor, it is...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang Y,Chou JH,Bradley J,Bargmann CI,Zinn K

更新日期：1997-10-28 00:00:00

abstract：:A key element for the development of suitable anti-cancer drugs is the identification of cancer-specific enzymatic activities that can be therapeutically targeted. Mucosa-associated lymphoid tissue transformation protein 1 (MALT1) is a proto-oncogene that contributes to tumorigenesis in diffuse large B-cell lymphoma (...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hailfinger S,Lenz G,Ngo V,Posvitz-Fejfar A,Rebeaud F,Guzzardi M,Penas EM,Dierlamm J,Chan WC,Staudt LM,Thome M

更新日期：2009-11-24 00:00:00

abstract：:There exist a limited but growing number of biological metal centers whose properties lie conspicuously outside the realm of known inorganic chemistry. The synthetic analogue approach, broadly directed, offers a powerful exploratory tool that can define intrinsic chemical possibilities for these sites while simultaneo...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee SC,Holm RH

更新日期：2003-04-01 00:00:00

abstract：:There is no effective treatment for the cardiomyopathy of the most common autosomal recessive ataxia, Friedreich's ataxia (FA). The identification of potentially toxic mitochondrial (MIT) iron (Fe) deposits in FA suggests that Fe plays a role in its pathogenesis. This study used the muscle creatine kinase conditional ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Whitnall M,Suryo Rahmanto Y,Sutak R,Xu X,Becker EM,Mikhael MR,Ponka P,Richardson DR

更新日期：2008-07-15 00:00:00

abstract：:A synchronized heart beat is controlled by pacemaking impulses conducted through Purkinje fibers. In chicks, these impulse-conducting cells are recruited during embryogenesis from myocytes in direct association with developing coronary arteries. In culture, the vascular cytokine endothelin converts embryonic myocytes ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hyer J,Johansen M,Prasad A,Wessels A,Kirby ML,Gourdie RG,Mikawa T

更新日期：1999-11-09 00:00:00