当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Deactivation kinetics of acid-sensing ion channel 1a are strongly pH-sensitive.

Deactivation kinetics of acid-sensing ion channel 1a are strongly pH-sensitive.

Abstract：

:Acid-sensing ion channels (ASICs) are trimeric cation-selective ion channels activated by protons in the physiological range. Recent reports have revealed that postsynaptically localized ASICs contribute to the excitatory postsynaptic current by responding to the transient acidification of the synaptic cleft that accompanies neurotransmission. In response to such brief acidic transients, both recombinant and native ASICs show extremely rapid deactivation in outside-out patches when jumping from a pH 5 stimulus to a single resting pH of 8. Given that the resting pH of the synaptic cleft is highly dynamic and depends on recent synaptic activity, we explored the kinetics of ASIC1a and 1a/2a heteromers to such brief pH transients over a wider [H+] range to approximate neuronal conditions better. Surprisingly, the deactivation of ASICs was steeply dependent on the pH, spanning nearly three orders of magnitude from extremely fast (<1 ms) at pH 8 to very slow (>300 ms) at pH 7. This study provides an example of a ligand-gated ion channel whose deactivation is sensitive to agonist concentrations that do not directly activate the receptor. Kinetic simulations and further mutagenesis provide evidence that ASICs show such steeply agonist-dependent deactivation because of strong cooperativity in proton binding. This capacity to signal across such a large synaptically relevant bandwidth enhances the response to small-amplitude acidifications likely to occur at the cleft and may provide ASICs with the ability to shape activity in response to the recent history of the synapse.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

MacLean DM,Jayaraman V

doi

10.1073/pnas.1620508114

subject

Has Abstract

pub_date

2017-03-21 00:00:00

pages

E2504-E2513

issue

12

eissn

0027-8424

issn

1091-6490

pii

1620508114

journal_volume

114

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Activation of the epsilon isoform of protein kinase C in the mammalian nerve terminal.

    abstract：:Activation of protein kinase C (PKC) by phorbol ester facilitates hormonal secretion and transmitter release, and phorbol ester-induced synaptic potentiation (PESP) is a model for presynaptic facilitation. A variety of PKC isoforms are expressed in the central nervous system, but the isoform involved in the PESP has n...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.241333598

    authors： Saitoh N,Hori T,Takahashi T

    更新日期：2001-11-20 00:00:00

  • Mycobacterium tuberculosis glycosylated phosphatidylinositol causes phagosome maturation arrest.

    abstract：:The tubercle bacillus parasitizes macrophages by inhibiting phagosome maturation into the phagolysosome. This phenomenon underlies the tuberculosis pandemic involving 2 billion people. We report here how Mycobacterium tuberculosis causes phagosome maturation arrest. A glycosylated M. tuberculosis phosphatidylinositol ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0737613100

    authors： Fratti RA,Chua J,Vergne I,Deretic V

    更新日期：2003-04-29 00:00:00

  • Recombinant human Fab fragments neutralize human type 1 immunodeficiency virus in vitro.

    abstract：:A panel of 20 recombinant Fab fragments reactive with the surface glycoprotein gp120 of human type 1 immunodeficiency virus (HIV-1) were examined for their ability to neutralize MN and IIIB strains of the virus. Neutralization was determined as the ability of the Fab fragments to inhibit infection as measured in both ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.19.9339

    authors： Barbas CF 3rd,Björling E,Chiodi F,Dunlop N,Cababa D,Jones TM,Zebedee SL,Persson MA,Nara PL,Norrby E

    更新日期：1992-10-01 00:00:00

  • An original adaptation of photosynthesis in the marine green alga Ostreococcus.

    abstract：:Adaptation of photosynthesis in marine environment has been examined in two strains of the green, picoeukaryote Ostreococcus: OTH95, a surface/high-light strain, and RCC809, a deep-sea/low-light strain. Differences between the two strains include changes in the light-harvesting capacity, which is lower in OTH95, and i...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0802762105

    authors： Cardol P,Bailleul B,Rappaport F,Derelle E,Béal D,Breyton C,Bailey S,Wollman FA,Grossman A,Moreau H,Finazzi G

    更新日期：2008-06-03 00:00:00

  • Enhanced chemistry-climate feedbacks in past greenhouse worlds.

    abstract：:Trace greenhouse gases are a fundamentally important component of Earth's global climate system sensitive to global change. However, their concentration in the pre-Pleistocene atmosphere during past warm greenhouse climates is highly uncertain because we lack suitable geochemical or biological proxies. This long-stand...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1102409108

    authors： Beerling DJ,Fox A,Stevenson DS,Valdes PJ

    更新日期：2011-06-14 00:00:00

  • Functional genetic expression of eukaryotic DNA in Escherichia coli.

    abstract：:We have isolated a segment of DNA from the eukaryote Saccharomyces cerevisiae (baker's yeast) as a viable molecular hybrid of bacteriophage lambda DNA which, when integrated into the chromosome of an E. coli histidine auxotroph, allows this bacterium to grow in the absence of histidine. The nonrevertable, histidine au...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.73.5.1471

    authors： Struhl K,Cameron JR,Davis RW

    更新日期：1976-05-01 00:00:00

  • A voltage-driven switch for ion-independent signaling by ether-à-go-go K+ channels.

    abstract：:Voltage-gated channels maintain cellular resting potentials and generate neuronal action potentials by regulating ion flux. Here, we show that Ether-à-go-go (EAG) K+ channels also regulate intracellular signaling pathways by a mechanism that is independent of ion flux and depends on the position of the voltage sensor....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0505909103

    authors： Hegle AP,Marble DD,Wilson GF

    更新日期：2006-02-21 00:00:00

  • Effects of past, present, and future ocean carbon dioxide concentrations on the growth and survival of larval shellfish.

    abstract：:The combustion of fossil fuels has enriched levels of CO(2) in the world's oceans and decreased ocean pH. Although the continuation of these processes may alter the growth, survival, and diversity of marine organisms that synthesize CaCO(3) shells, the effects of ocean acidification since the dawn of the industrial re...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0913804107

    authors： Talmage SC,Gobler CJ

    更新日期：2010-10-05 00:00:00

  • Functional characterization of piggyBat from the bat Myotis lucifugus unveils an active mammalian DNA transposon.

    abstract：:A revelation of the genomic age has been the contributions of the mobile DNA segments called transposable elements to chromosome structure, function, and evolution in virtually all organisms. Substantial fractions of vertebrate genomes derive from transposable elements, being dominated by retroelements that move via R...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1217548110

    authors： Mitra R,Li X,Kapusta A,Mayhew D,Mitra RD,Feschotte C,Craig NL

    更新日期：2013-01-02 00:00:00

  • RET signaling does not modulate MPTP toxicity but is required for regeneration of dopaminergic axon terminals.

    abstract：:Activation of the RET (rearranged during transfection) receptor by glial cell-line-derived neurotrophic factor (GDNF) has been identified as an important differentiation and survival factor for dopaminergic neurons of the midbrain in preclinical experiments. These encouraging results have led to clinical trials of GDN...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0706177104

    authors： Kowsky S,Pöppelmeyer C,Kramer ER,Falkenburger BH,Kruse A,Klein R,Schulz JB

    更新日期：2007-12-11 00:00:00

  • Correlated dynamics of consecutive residues reveal transient and cooperative unfolding of secondary structure in proteins.

    abstract：:Nuclear spin relaxation is a powerful method for studying molecular dynamics at atomic resolution. Recent methods development in biomolecular NMR spectroscopy has enabled detailed investigations of molecular dynamics that are critical for biological function, with prominent examples addressing allostery, enzyme cataly...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0504361102

    authors： Lundström P,Mulder FA,Akke M

    更新日期：2005-11-22 00:00:00

  • A proposed common allosteric mechanism for active transport, muscle contraction, and ribosomal translocation.

    abstract：:It is suggested that active transport, muscle contraction, and ribosomal translocation may all make use of a common allosteric mechanism in which ATP or GTP serves as both the effector and substrate and in which a conformational change in a protein (enzyme) moves or exerts a force on a second ligand. The enzymatic spl...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.64.1.267

    authors： Hill TL

    更新日期：1969-09-01 00:00:00

  • Role of social wasps in Saccharomyces cerevisiae ecology and evolution.

    abstract：:Saccharomyces cerevisiae is one of the most important model organisms and has been a valuable asset to human civilization. However, despite its extensive use in the last 9,000 y, the existence of a seasonal cycle outside human-made environments has not yet been described. We demonstrate the role of social wasps as vec...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1208362109

    authors： Stefanini I,Dapporto L,Legras JL,Calabretta A,Di Paola M,De Filippo C,Viola R,Capretti P,Polsinelli M,Turillazzi S,Cavalieri D

    更新日期：2012-08-14 00:00:00

  • Gene activation in somatic nuclei after injection into amphibian oocytes.

    abstract：:Genes that are unexpressed in somatic cells have been activated by injecting cultured cell nuclei of the frog Xenopus laevis into oocytes of the newt Pleurodeles waltlii. The genes that were activated are normally expressed in oocytes but not in cultured cells. Conversely, genes that are normally expressed in cultured...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.6.2470

    authors： De Robertis EM,Gurdon JB

    更新日期：1977-06-01 00:00:00

  • High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization.

    abstract：:We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in l...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1313368111

    authors： Holden SJ,Pengo T,Meibom KL,Fernandez Fernandez C,Collier J,Manley S

    更新日期：2014-03-25 00:00:00

  • MicroRNA-126-mediated control of cell fate in B-cell myeloid progenitors as a potential alternative to transcriptional factors.

    abstract：:Lineage specification is thought to be largely regulated at the level of transcription, where lineage-specific transcription factors drive specific cell fates. MicroRNAs (miR), vital to many cell functions, act posttranscriptionally to decrease the expression of target mRNAs. MLL-AF4 acute lymphocytic leukemia exhibit...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1220710110

    authors： Okuyama K,Ikawa T,Gentner B,Hozumi K,Harnprasopwat R,Lu J,Yamashita R,Ha D,Toyoshima T,Chanda B,Kawamata T,Yokoyama K,Wang S,Ando K,Lodish HF,Tojo A,Kawamoto H,Kotani A

    更新日期：2013-08-13 00:00:00

  • Local adaptation in Trinidadian guppies alters ecosystem processes.

    abstract：:Theory suggests evolutionary change can significantly influence and act in tandem with ecological forces via ecological-evolutionary feedbacks. This theory assumes that significant evolutionary change occurs over ecologically relevant timescales and that phenotypes have differential effects on the environment. Here we...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0908023107

    authors： Bassar RD,Marshall MC,López-Sepulcre A,Zandonà E,Auer SK,Travis J,Pringle CM,Flecker AS,Thomas SA,Fraser DF,Reznick DN

    更新日期：2010-02-23 00:00:00

  • Immunoglobulins D and M mediate signals that are qualitatively different in B cells with an immature phenotype.

    abstract：:The CH family of murine B-cell lymphomas includes several members that are sensitive to growth inhibition when their membrane IgM (mIgM) receptors are cross-linked by anti-mu chain, anti-kappa chain, or anti-idiotypic antibodies. These lymphomas are IgM+, Ia+, and IgD +/- and resemble neonatal B cells in terms of thei...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.85.18.6919

    authors： Alés-Martínez JE,Warner GL,Scott DW

    更新日期：1988-09-01 00:00:00

  • Rapid evolution of male-biased gene expression in Drosophila.

    abstract：:A number of genes associated with sexual traits and reproduction evolve at the sequence level faster than the majority of genes coding for non-sex-related traits. Whole genome analyses allow this observation to be extended beyond the limited set of genes that have been studied thus far. We use cDNA microarrays to demo...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1630690100

    authors： Meiklejohn CD,Parsch J,Ranz JM,Hartl DL

    更新日期：2003-08-19 00:00:00

  • Allosteric activation of apicomplexan calcium-dependent protein kinases.

    abstract：:Calcium-dependent protein kinases (CDPKs) comprise the major group of Ca2+-regulated kinases in plants and protists. It has long been assumed that CDPKs are activated, like other Ca2+-regulated kinases, by derepression of the kinase domain (KD). However, we found that removal of the autoinhibitory domain from Toxoplas...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1505914112

    authors： Ingram JR,Knockenhauer KE,Markus BM,Mandelbaum J,Ramek A,Shan Y,Shaw DE,Schwartz TU,Ploegh HL,Lourido S

    更新日期：2015-09-08 00:00:00

  • Mixed-power scaling of whole-plant respiration from seedlings to giant trees.

    abstract：:The scaling of respiratory metabolism with body mass is one of the most pervasive phenomena in biology. Using a single allometric equation to characterize empirical scaling relationships and to evaluate alternative hypotheses about mechanisms has been controversial. We developed a method to directly measure respiratio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0902554107

    authors： Mori S,Yamaji K,Ishida A,Prokushkin SG,Masyagina OV,Hagihara A,Hoque AT,Suwa R,Osawa A,Nishizono T,Ueda T,Kinjo M,Miyagi T,Kajimoto T,Koike T,Matsuura Y,Toma T,Zyryanova OA,Abaimov AP,Awaya Y,Araki MG,Kawasaki T

    更新日期：2010-01-26 00:00:00

  • Evidence for a recent increase in forest growth.

    abstract：:Forests and their soils contain the majority of the earth's terrestrial carbon stocks. Changes in patterns of tree growth can have a huge impact on atmospheric cycles, biogeochemical cycles, climate change, and biodiversity. Recent studies have shown increases in biomass across many forest types. This increase has bee...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0912376107

    authors： McMahon SM,Parker GG,Miller DR

    更新日期：2010-02-23 00:00:00

  • How stereotypes impair women's careers in science.

    abstract：:Women outnumber men in undergraduate enrollments, but they are much less likely than men to major in mathematics or science or to choose a profession in these fields. This outcome often is attributed to the effects of negative sex-based stereotypes. We studied the effect of such stereotypes in an experimental market, ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1314788111

    authors： Reuben E,Sapienza P,Zingales L

    更新日期：2014-03-25 00:00:00

  • Regulation of the galactose pathway in Saccharomyces cerevisiae: induction of uridyl transferase mRNA and dependency on GAL4 gene function.

    abstract：:In Saccharomyces cerevisiae, utilization of galactose requires four inducible enzyme activities. Three of these activities (galactose-1-phosphate uridyl transferase, EC 2.7.7.10; uridine diphosphogalactose 4-epimerase, EC 5.1.3.2; and galactokinase, EC 2.7.1.6) are specified by three tightly linked genes (GAL7, GAL10,...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.75.6.2878

    authors： Hopper JE,Broach JR,Rowe LB

    更新日期：1978-06-01 00:00:00

  • An auxin-driven polarized transport model for phyllotaxis.

    abstract：:Recent studies show that plant organ positioning may be mediated by localized concentrations of the plant hormone auxin. Auxin patterning in the shoot apical meristem is in turn brought about by the subcellular polar distribution of the putative auxin efflux mediator, PIN1. However, the question of what signals determ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0509839103

    authors： Jönsson H,Heisler MG,Shapiro BE,Meyerowitz EM,Mjolsness E

    更新日期：2006-01-31 00:00:00

  • Effects of environmental stressors on daily governance.

    abstract：:Human workers ensure the functioning of governments around the world. The efficacy of human workers, in turn, is linked to the climatic conditions they face. Here we show that the same weather that amplifies human health hazards also reduces street-level government workers' oversight of these hazards. To do so, we emp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1803765115

    authors： Obradovich N,Tingley D,Rahwan I

    更新日期：2018-08-28 00:00:00

  • Prospective measurements of dehydroepiandrosterone sulfate in a cohort of elderly subjects: relationship to gender, subjective health, smoking habits, and 10-year mortality.

    abstract：:The decrease with age of the adrenal-secreted dehydroepiandrosterone sulfate (DHEAS) in serum has suggested that it may be causally related to longevity. For the PAQUID [People (Personnes) Aged (Agées) About What (Quid, in Latin)] cohort of elderly subjects, we have previously reported higher DHEAS in men than in wome...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.121177998

    authors： Mazat L,Lafont S,Berr C,Debuire B,Tessier JF,Dartigues JF,Baulieu EE

    更新日期：2001-07-03 00:00:00

  • Deficiency of acyl CoA:cholesterol acyltransferase 2 prevents atherosclerosis in apolipoprotein E-deficient mice.

    abstract：:Deficiency of acyl CoA:cholesterol acyltransferase 2 (ACAT2) in mice results in a reduction in cholesterol ester synthesis in the small intestine and liver, which in turn limits intestinal cholesterol absorption, hepatic cholesterol gallstone formation, and the accumulation of cholesterol esters in the plasma lipoprot...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0336398100

    authors： Willner EL,Tow B,Buhman KK,Wilson M,Sanan DA,Rudel LL,Farese RV Jr

    更新日期：2003-02-04 00:00:00

  • Potent inhibition of huntingtin aggregation and cytotoxicity by a disulfide bond-free single-domain intracellular antibody.

    abstract：:Huntington's disease (HD) is a progressive neurodegenerative disorder caused by an expansion in the number of polyglutamine-encoding CAG repeats in the gene that encodes the huntingtin (htt) protein. A property of the mutant protein that is intimately involved in the development of the disease is the propensity of the...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0408134101

    authors： Colby DW,Chu Y,Cassady JP,Duennwald M,Zazulak H,Webster JM,Messer A,Lindquist S,Ingram VM,Wittrup KD

    更新日期：2004-12-21 00:00:00

  • Monopoles and fractional vortices in chiral superconductors.

    abstract：:I discuss two exotic objects that must be experimentally identified in chiral superfluids and superconductors. These are (i) the vortex with a fractional quantum number (N = 1/2 in chiral superfluids, and N = 1/2 and N = 1/4 in chiral superconductors), which plays the part of the Alice string in relativistic theories ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.97.6.2431

    authors： Volovik GE

    更新日期：2000-03-14 00:00:00