High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization.

Abstract:

:We created a high-throughput modality of photoactivated localization microscopy (PALM) that enables automated 3D PALM imaging of hundreds of synchronized bacteria during all stages of the cell cycle. We used high-throughput PALM to investigate the nanoscale organization of the bacterial cell division protein FtsZ in live Caulobacter crescentus. We observed that FtsZ predominantly localizes as a patchy midcell band, and only rarely as a continuous ring, supporting a model of "Z-ring" organization whereby FtsZ protofilaments are randomly distributed within the band and interact only weakly. We found evidence for a previously unidentified period of rapid ring contraction in the final stages of the cell cycle. We also found that DNA damage resulted in production of high-density continuous Z-rings, which may obstruct cytokinesis. Our results provide a detailed quantitative picture of in vivo Z-ring organization.

authors

Holden SJ,Pengo T,Meibom KL,Fernandez Fernandez C,Collier J,Manley S

doi

10.1073/pnas.1313368111

subject

Has Abstract

pub_date

2014-03-25 00:00:00

pages

4566-71

issue

12

eissn

0027-8424

issn

1091-6490

pii

1313368111

journal_volume

111

pub_type

杂志文章
  • Ethionamide activation and sensitivity in multidrug-resistant Mycobacterium tuberculosis.

    abstract::Ethionamide (ETA) is an important component of second-line therapy for the treatment of multidrug-resistant tuberculosis. Synthesis of radiolabeled ETA and an examination of drug metabolites formed by whole cells of Mycobacterium tuberculosis (MTb) have allowed us to demonstrate that ETA is activated by S-oxidation be...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.97.17.9677

    authors: DeBarber AE,Mdluli K,Bosman M,Bekker LG,Barry CE 3rd

    更新日期:2000-08-15 00:00:00

  • Probing the instabilities in the dynamics of helical fragments from mouse PrPC.

    abstract::The first step in the formation of the protease resistant form (PrPSc) of prion proteins involves a conformational transition of the monomeric cellular form of PrPC to a more stable aggregation prone state PrPC*. A search of PDBselect and Escherichia coli and yeast genomes shows that the exact pattern of charges in he...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0404235101

    authors: Dima RI,Thirumalai D

    更新日期:2004-10-26 00:00:00

  • Separation between cytosolic calcium and secretion in chromaffin cells superfused with calcium ramps.

    abstract::This paper describes experiments in which cytosolic Ca2+ concentrations ([Ca2+]i) and catecholamine release were measured in two populations of chromaffin cells stimulated with a solution enriched in K+ (100 mM). Once depolarized, external Ca2+ or Ba2+ ions were offered to cells either as a single 2.5 mM step or as a ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.90.8.3284

    authors: Michelena P,García-Pérez LE,Artalejo AR,García AG

    更新日期:1993-04-15 00:00:00

  • Induction of colitis causes inflammatory responses in fat depots: evidence for substance P pathways in human mesenteric preadipocytes.

    abstract::Intracolonic administration of trinitrobenzene sulfonic acid in mice causes inflammation in the colon that is accompanied by increased expression of proinflammatory cytokines and of the substance P (SP), neurokinin 1 receptor (NK-1R) in the proximal mesenteric fat depot. We also investigated whether human mesenteric p...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0600821103

    authors: Karagiannides I,Kokkotou E,Tansky M,Tchkonia T,Giorgadze N,O'Brien M,Leeman SE,Kirkland JL,Pothoulakis C

    更新日期:2006-03-28 00:00:00

  • Ergodic and nonergodic processes coexist in the plasma membrane as observed by single-molecule tracking.

    abstract::Diffusion in the plasma membrane of living cells is often found to display anomalous dynamics. However, the mechanism underlying this diffusion pattern remains highly controversial. Here, we study the physical mechanism underlying Kv2.1 potassium channel anomalous dynamics using single-molecule tracking. Our analysis ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1016325108

    authors: Weigel AV,Simon B,Tamkun MM,Krapf D

    更新日期:2011-04-19 00:00:00

  • Binding between the Niemann-Pick C1 protein and a photoactivatable cholesterol analog requires a functional sterol-sensing domain.

    abstract::Niemann-Pick type C (NPC) 1 protein plays important roles in moving cholesterol and other lipids out of late endosomes by means of vesicular trafficking, but it is not known whether NPC1 directly interacts with cholesterol. We performed photoaffinity labeling of intact cells expressing fluorescent protein (FP)-tagged ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0405255101

    authors: Ohgami N,Ko DC,Thomas M,Scott MP,Chang CC,Chang TY

    更新日期:2004-08-24 00:00:00

  • Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan:galacturonosyltransferase complex.

    abstract::Plant cell wall pectic polysaccharides are arguably the most complex carbohydrates in nature. Progress in understanding pectin synthesis has been slow due to its complex structure and difficulties in purifying and expressing the low-abundance, Golgi membrane-bound pectin biosynthetic enzymes. Arabidopsis galacturonosy...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1112816108

    authors: Atmodjo MA,Sakuragi Y,Zhu X,Burrell AJ,Mohanty SS,Atwood JA 3rd,Orlando R,Scheller HV,Mohnen D

    更新日期:2011-12-13 00:00:00

  • Identification of the Fc gamma receptor class I binding site in human IgG through the use of recombinant IgG1/IgG2 hybrid and point-mutated antibodies.

    abstract::To characterize the region on human IgG1 responsible for its high-affinity interaction with the human Fc gamma receptor class I (Fc gamma RI), we have analyzed the binding properties of a series of genetically engineered chimeric antidinitrophenyl antibodies with identical murine antibody combining sites and hybrid Ig...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.20.9036

    authors: Chappel MS,Isenman DE,Everett M,Xu YY,Dorrington KJ,Klein MH

    更新日期:1991-10-15 00:00:00

  • Knockin of mutant PIK3CA activates multiple oncogenic pathways.

    abstract::The phosphatidylinositol 3-kinase subunit PIK3CA is frequently mutated in human cancers. Here we used gene targeting to "knock in" PIK3CA mutations into human breast epithelial cells to identify new therapeutic targets associated with oncogenic PIK3CA. Mutant PIK3CA knockin cells were capable of epidermal growth facto...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0813351106

    authors: Gustin JP,Karakas B,Weiss MB,Abukhdeir AM,Lauring J,Garay JP,Cosgrove D,Tamaki A,Konishi H,Konishi Y,Mohseni M,Wang G,Rosen DM,Denmeade SR,Higgins MJ,Vitolo MI,Bachman KE,Park BH

    更新日期:2009-02-24 00:00:00

  • Hydrogen sulfide (H2S) metabolism in mitochondria and its regulatory role in energy production.

    abstract::Although many types of ancient bacteria and archea rely on hydrogen sulfide (H(2)S) for their energy production, eukaryotes generate ATP in an oxygen-dependent fashion. We hypothesize that endogenous H(2)S remains a regulator of energy production in mammalian cells under stress conditions, which enables the body to co...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1115634109

    authors: Fu M,Zhang W,Wu L,Yang G,Li H,Wang R

    更新日期:2012-02-21 00:00:00

  • The selection of acceptable protein mutations.

    abstract::We have determined the general constraints that govern sequence divergence in proteins that retain entirely, or very largely, the same structure and function. To do this we collected data from three different groups of orthologous sequences: those found in humans and mice, in humans and chickens, and in Escherichia co...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0703737104

    authors: Sasidharan R,Chothia C

    更新日期:2007-06-12 00:00:00

  • Structure of a cytochrome P450-redox partner electron-transfer complex.

    abstract::The crystal structure of the complex between the heme- and FMN-binding domains of bacterial cytochrome P450BM-3, a prototype for the complex between eukaryotic microsomal P450s and P450 reductase, has been determined at 2.03 A resolution. The flavodoxin-like flavin domain is positioned at the proximal face of the heme...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.96.5.1863

    authors: Sevrioukova IF,Li H,Zhang H,Peterson JA,Poulos TL

    更新日期:1999-03-02 00:00:00

  • Genomewide transcriptional changes associated with genetic alterations and nutritional supplementation affecting tryptophan metabolism in Bacillus subtilis.

    abstract::DNA microarrays comprising approximately 95% of the Bacillus subtilis annotated protein coding ORFs were deployed to generate a series of snapshots of genomewide transcriptional changes that occur when cells are grown under various conditions that are expected to increase or decrease transcription of the trp operon se...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1031606100

    authors: Berka RM,Cui X,Yanofsky C

    更新日期:2003-05-13 00:00:00

  • Chloroplast DNA inversions and the origin of the grass family (Poaceae).

    abstract::The phylogenetic affinities of the grass family (Poaceae) have long been debated. The chloroplast genomes of at least some grasses have been known to possess three inversions relative to the typical gene arrangement found in most flowering plants. We have surveyed for the presence of these inversions in grasses and ot...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.89.16.7722

    authors: Doyle JJ,Davis JI,Soreng RJ,Garvin D,Anderson MJ

    更新日期:1992-08-15 00:00:00

  • A role for Arf1 in mitotic Golgi disassembly, chromosome segregation, and cytokinesis.

    abstract::In mitosis, chromosome, cytoskeleton, and organelle dynamics must be coordinated for successful cell division. Here, we present evidence for a role for Arf1, a small GTPase associated with the Golgi apparatus, in the orchestration of mitotic Golgi breakdown, chromosome segregation, and cytokinesis. We show that early ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.2234055100

    authors: Altan-Bonnet N,Phair RD,Polishchuk RS,Weigert R,Lippincott-Schwartz J

    更新日期:2003-11-11 00:00:00

  • Expression of GATA-binding proteins during embryonic development in Xenopus laevis.

    abstract::Proteins that recognize the core sequence GATA are important regulators of hematopoietic-specific gene transcription. We have characterized cDNAs encoding the Xenopus laevis homologues of three related transcription factors, designated GATA-1, -2, and -3. Comparative sequence analysis reveals strong conservation of th...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.23.10642

    authors: Zon LI,Mather C,Burgess S,Bolce ME,Harland RM,Orkin SH

    更新日期:1991-12-01 00:00:00

  • Different mouse mast cell populations express various combinations of at least six distinct mast cell serine proteases.

    abstract::Mouse serosal mast cells (SMCs) and Kirsten sarcoma virus-immortalized mast cells store large amounts of mast cell carboxypeptidase A and serine proteases in their secretory granules. Secretory granule proteins from 2.6 x 10(6) purified SMCs were separated by NaDodSO4/PAGE, trans-blotted to poly(vinylidine difluoride)...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.8.3230

    authors: Reynolds DS,Stevens RL,Lane WS,Carr MH,Austen KF,Serafin WE

    更新日期:1990-04-01 00:00:00

  • Oscillatory activity in the infant brain reflects object maintenance.

    abstract::The apparent failure of infants to understand "object permanence" by reaching for hidden objects is perhaps the most striking and debated phenomenon in cognitive development. Of particular interest is the extent to which infants perceive and remember objects in a similar way to that of adults. Here we report two findi...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0507626102

    authors: Kaufman J,Csibra G,Johnson MH

    更新日期:2005-10-18 00:00:00

  • Role of the hypothalamus in the control of atrial natriuretic peptide release.

    abstract::Stimulation of the region antero-ventral to the third cerebral ventricle (AV3V) by a cholinergic drug, carbachol, and lesions of the AV3V have been demonstrated in previous studies to either augment or decrease sodium excretion, respectively. Atrial natriuretic peptide (ANP) dramatically increases renal sodium excreti...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.23.9621

    authors: Baldissera S,Menani JW,dos Santos LF,Favaretto AL,Gutkowska J,Turrin MQ,McCann SM,Antunes-Rodrigues J

    更新日期:1989-12-01 00:00:00

  • Structural basis for the extended CAP-Gly domains of p150(glued) binding to microtubules and the implication for tubulin dynamics.

    abstract::p150(glued) belongs to a group of proteins accumulating at microtubule plus ends (+TIPs). It plays a key role in initiating retrograde transport by recruiting and tethering endosomes and dynein to microtubules. p150(glued) contains an N-terminal microtubule-binding cytoskeleton-associated protein glycine-rich (CAP-Gly...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1403135111

    authors: Wang Q,Crevenna AH,Kunze I,Mizuno N

    更新日期:2014-08-05 00:00:00

  • Detection of atomic spin labels in a lipid bilayer using a single-spin nanodiamond probe.

    abstract::Magnetic field fluctuations arising from fundamental spins are ubiquitous in nanoscale biology, and are a rich source of information about the processes that generate them. However, the ability to detect the few spins involved without averaging over large ensembles has remained elusive. Here, we demonstrate the detect...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1300640110

    authors: Kaufmann S,Simpson DA,Hall LT,Perunicic V,Senn P,Steinert S,McGuinness LP,Johnson BC,Ohshima T,Caruso F,Wrachtrup J,Scholten RE,Mulvaney P,Hollenberg L

    更新日期:2013-07-02 00:00:00

  • Cooperation and control in multiplayer social dilemmas.

    abstract::Direct reciprocity and conditional cooperation are important mechanisms to prevent free riding in social dilemmas. However, in large groups, these mechanisms may become ineffective because they require single individuals to have a substantial influence on their peers. However, the recent discovery of zero-determinant ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1407887111

    authors: Hilbe C,Wu B,Traulsen A,Nowak MA

    更新日期:2014-11-18 00:00:00

  • Binding and transcription of relaxed DNA templates by fractions of maize chloroplast extracts.

    abstract::Preparations of partially purified chloroplast DNA-dependent RNA polymerase from maize and some other plants transcribe cloned chloroplast genes preferentially and much more actively from appropriately negatively supercoiled templates than from relaxed templates. We have found that the polymerase in such fractions doe...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.86.3.876

    authors: Zaitlin D,Hu J,Bogorad L

    更新日期:1989-02-01 00:00:00

  • Characterization of cultured rat oligodendrocytes proliferating in a serum-free, chemically defined medium.

    abstract::A serumless, chemically defined medium has been developed for the culture of oligodendrocytes isolated from primary neonatal rat cerebral cultures. Combined together, insulin, transferrin, and fibroblast growth factor synergistically induced an essentially homogenous population (95-98%) of cells expressing glycerol-3-...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.82.10.3509

    authors: Saneto RP,de Vellis J

    更新日期:1985-05-01 00:00:00

  • Higher-Order Curvature and Local Solvability of D(theta).

    abstract::Let E and F be vector bundles and D:E --> F an operator of order k. We associate a sequence of invariants ((l))(D), l >/= 0 with D which generalize the concept of curvature in a natural way. In the case where D = D(theta) is the differential operator of a connection theta on a vector bundle E, ((1))(D(theta)) is the c...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.70.3.797

    authors: Knapp RE

    更新日期:1973-03-01 00:00:00

  • A new high molecular weight immunoglobulin class from the carcharhine shark: implications for the properties of the primordial immunoglobulin.

    abstract::All immunoglobulins and T-cell receptors throughout phylogeny share regions of highly conserved amino acid sequence. To identify possible primitive immunoglobulins and immunoglobulin-like molecules, we utilized 3' RACE (rapid amplification of cDNA ends) and a highly conserved constant region consensus amino acid seque...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.93.8.3289

    authors: Berstein RM,Schluter SF,Shen S,Marchalonis JJ

    更新日期:1996-04-16 00:00:00

  • A perceptual metric for photo retouching.

    abstract::In recent years, advertisers and magazine editors have been widely criticized for taking digital photo retouching to an extreme. Impossibly thin, tall, and wrinkle- and blemish-free models are routinely splashed onto billboards, advertisements, and magazine covers. The ubiquity of these unrealistic and highly idealize...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1110747108

    authors: Kee E,Farid H

    更新日期:2011-12-13 00:00:00

  • Selective growth in serum-free hormone-supplemented medium of tumor cells obtained by biopsy from patients with small cell carcinoma of the lung.

    abstract::Fifteen tumor-containing specimens were obtained directly from patients with small cell carcinoma of the lung and tested for their ability to grow in serum-supplemented medium and in serum-free medium supplemented with hydrocortisone, insulin, transferrin, estrogen, and selenium (HITES). The tumor cells replicated in ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.5.3185

    authors: Carney DN,Bunn PA Jr,Gazdar AF,Pagan JA,Minna JD

    更新日期:1981-05-01 00:00:00

  • Molecular mapping of the heparin-binding exosite of thrombin.

    abstract::Thrombin contains electropositive patches at opposite poles of the molecule which represent potential exosites for the binding of macromolecular ligands. The function of anion-binding exosite I, the fibrin(ogen) recognition site, has been well described. Anion-binding exosite II, located near the carboxyl terminus of ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.12.5518

    authors: Sheehan JP,Sadler JE

    更新日期:1994-06-07 00:00:00

  • Adaptive evolution of cytochrome c oxidase subunit VIII in anthropoid primates.

    abstract::Cytochrome c oxidase (COX) is a 13-subunit protein complex that catalyzes the last step in mitochondrial electron transfer in mammals. Of the 10 subunits encoded by nuclear DNA (three are mtDNA products), some are expressed as tissue- and/or development-specific isoforms. For COX subunit VIII, previous work showed tha...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0931463100

    authors: Goldberg A,Wildman DE,Schmidt TR,Huttemann M,Goodman M,Weiss ML,Grossman LI

    更新日期:2003-05-13 00:00:00