Abstract:
:We have isolated a segment of DNA from the eukaryote Saccharomyces cerevisiae (baker's yeast) as a viable molecular hybrid of bacteriophage lambda DNA which, when integrated into the chromosome of an E. coli histidine auxotroph, allows this bacterium to grow in the absence of histidine. The nonrevertable, histidine auxotroph lacks the enzymatic activity of imidazole glycerol phosphate (IGP) dehydratase (EC 4.2.1.19). From genetic experiments, we conclude that expression of the segment of yeast DNA results in the production of a diffusible substance and that transcription necessary for the complementation is most likely initiated from the segment of eukaryotic DNA.
journal_name
Proc Natl Acad Sci U S Aauthors
Struhl K,Cameron JR,Davis RWdoi
10.1073/pnas.73.5.1471subject
Has Abstractpub_date
1976-05-01 00:00:00pages
1471-5issue
5eissn
0027-8424issn
1091-6490journal_volume
73pub_type
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