Recombinant human Fab fragments neutralize human type 1 immunodeficiency virus in vitro.

abstract：:Therapeutic cancer vaccines can be effective for treating patients, but clinical responses vary considerably from patient to patient. Early indicators of a favorable response are crucial for making individualized treatment decisions and advancing vaccine design, but no validated biomarkers are currently available. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Campbell CT,Gulley JL,Oyelaran O,Hodge JW,Schlom J,Gildersleeve JC

更新日期：2014-04-29 00:00:00

abstract：:Although it is known that the c-myc oncogene is rearranged in a head-to-head fashion with the immunoglobulin heavy chain locus in mouse plasmacytomas, it has not been clear whether the c-myc oncogene is translocated to the heavy chain locus on mouse chromosome 12 or whether the heavy chain locus is translocated to the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Erikson J,Miller DA,Miller OJ,Abcarian PW,Skurla RM,Mushinski JF,Croce CM

更新日期：1985-06-01 00:00:00

abstract：:We are designing simple peptide ion channels as model systems for the study of the physical principles controlling conduction through ion-channel proteins. Here we report on an uncharged peptide, Ac-(Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2, designed to form an aggregate of parallel, amphiphilic, membrane-spanning alpha-he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kienker PK,DeGrado WF,Lear JD

更新日期：1994-05-24 00:00:00

abstract：:A method is described for monitoring the enzyme-mediated unwinding of duplex DNA spectrophotometrically. The assay employs a partially duplex oligonucleotide substrate modified at the complementary end with coumarin and fluorescein moieties. When in close proximity the fluorescein quenches the fluorescence of coumarin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Houston P,Kodadek T

更新日期：1994-06-07 00:00:00

abstract：:The phosphodiester bond formation by DNA-dependent RNA-polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) can in principle result in retention, inversion, or racemization of configuration at the alpha-phosphorus of the nucleoside 5'-triphosphate being polymerized. As...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Eckstein F,Armstrong VW,Sternbach H

更新日期：1976-09-01 00:00:00

abstract：:Indirect immunofluorescent analysis revealed that sera from five patients with subacute sclerosing panencephalitis possessed IgD antibodies directed against measles virus components in persistently infected HeLa cells. IgD levels in these sera were within the normal range. Control studies indicated that the reaction w...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luster MI,Armen RC,Hallum JV,Leslie GA

更新日期：1976-04-01 00:00:00

abstract：:Many bacteria use acyl homoserine lactone signals to monitor cell density in a type of gene regulation termed quorum sensing and response. Synthesis of these signals is directed by homologs of the luxi gene of Vibrio fischeri. This communication resolves two critical issues concerning the synthesis of the V. fischeri ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schaefer AL,Val DL,Hanzelka BL,Cronan JE Jr,Greenberg EP

更新日期：1996-09-03 00:00:00

abstract：:Dysregulation of signaling pathways in multiple sclerosis (MS) can be analyzed by phosphoproteomics in peripheral blood mononuclear cells (PBMCs). We performed in vitro kinetic assays on PBMCs in 195 MS patients and 60 matched controls and quantified the phosphorylation of 17 kinases using xMAP assays. Phosphoprotein ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kotelnikova E,Kiani NA,Messinis D,Pertsovskaya I,Pliaka V,Bernardo-Faura M,Rinas M,Vila G,Zubizarreta I,Pulido-Valdeolivas I,Sakellaropoulos T,Faigle W,Silberberg G,Masso M,Stridh P,Behrens J,Olsson T,Martin R,Paul F

更新日期：2019-05-07 00:00:00

abstract：:Ovine pulmonary surfactant is bactericidal for Pasteurella haemolytica when surfactant and bacteria mixtures are incubated with normal ovine serum. To isolate this component, surfactant (1 mg/ml) was centrifuged at 100,000 x gav, and the supernatant was fractionated by HPLC. Fractions were eluted with acetonitrile (10...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brogden KA,De Lucca AJ,Bland J,Elliott S

更新日期：1996-01-09 00:00:00

abstract：:Mutations in the iPLA2-VIA/PLA2G6 gene are responsible for PARK14-linked Parkinson's disease (PD) with α-synucleinopathy. However, it is unclear how iPLA2-VIA mutations lead to α-synuclein (α-Syn) aggregation and dopaminergic (DA) neurodegeneration. Here, we report that iPLA2-VIA-deficient Drosophila exhibits defects ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mori A,Hatano T,Inoshita T,Shiba-Fukushima K,Koinuma T,Meng H,Kubo SI,Spratt S,Cui C,Yamashita C,Miki Y,Yamamoto K,Hirabayashi T,Murakami M,Takahashi Y,Shindou H,Nonaka T,Hasegawa M,Okuzumi A,Imai Y,Hattori N

更新日期：2019-10-08 00:00:00

abstract：:The E1A gene of adenovirus type 5 encodes two major proteins of 289 and 243 amino acid residues, which are identical except that the larger protein has an internal stretch of 46 amino acids required for efficient trans-activation of early viral promoters. This domain contains a consensus zinc finger motif (Cys-Xaa2-Cy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Culp JS,Webster LC,Friedman DJ,Smith CL,Huang WJ,Wu FY,Rosenberg M,Ricciardi RP

更新日期：1988-09-01 00:00:00

abstract：:The recently discovered potato tuber (Solanum tuberosum) alpha-glucan, water dikinase (GWD) (formerly known as R1) catalyzes the phosphorylation of starch by a dikinase-type reaction mechanism in which the beta-phosphate of ATP is transferred to either the C-6 or the C-3 position of the glucosyl residue of starch. In ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mikkelsen R,Mutenda KE,Mant A,Schürmann P,Blennow A

更新日期：2005-02-01 00:00:00

abstract：:A perversion in an otherwise uniform helical structure, such as a climbing plant tendril, refers to a kink that connects two helices with opposite chiralities. Such singularity structures are widely seen in natural and artificial mechanical systems, and they provide the fundamental mechanism of helical symmetry breaki...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu S,Yao Z,Chiou K,Stupp SI,Olvera de la Cruz M

更新日期：2016-06-28 00:00:00

abstract：:Although the apoptotic role of caspases has been largely understood, accumulating evidence in Drosophila suggests that caspases also control other processes than apoptotic cell death. However, how caspases contribute to the development of the mammalian nervous system remains obscure. Here, we provide unique evidence t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohsawa S,Hamada S,Kuida K,Yoshida H,Igaki T,Miura M

更新日期：2010-07-27 00:00:00

abstract：:Visual awareness has been proposed to depend on recurrent processing in early visual cortex areas including the primary visual cortex (V1). Here, we address this hypothesis with high spatiotemporal resolution magnetoencephalographic recordings in subjects performing a substitution masking paradigm. Neural activity ref...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boehler CN,Schoenfeld MA,Heinze HJ,Hopf JM

更新日期：2008-06-24 00:00:00

abstract：:Bitemporal injections of puromycin that primarily affect the hippocampal-entorhinal area induce amnesia of aversive maze-learning in mice for 3 days after training but are ineffective 6 or more days after training. At these later times, additional puromycin sites covering widespread forebrain areas are necessary to in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Flexner JB,Flexner LB,Church AC,Rainbow TC,Brunswick DJ

更新日期：1985-11-01 00:00:00

abstract：:Continuous intravenous infusion of heparin suppresses smooth muscle cell proliferation in rats after endothelial injury but may lead to hemorrhage and other complications. The anticoagulant property has been removed from chemically modified heparin without loss of antiproliferative effect but use of such compounds is ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Edelman ER,Adams DH,Karnovsky MJ

更新日期：1990-05-01 00:00:00

abstract：:A compound of simple structure, 2,4-dichlorobenzyl thiocyanate (DCBT), is an antimitotic agent with a number of unusual properties. The drug causes an extreme reorganization of microtubules in cells in culture. Most normal microtubules disappear, and remaining tubulin-containing structures appear to be bundled or aggr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Abraham I,Dion RL,Chi DM,Gottesman MM,Hamel E

更新日期：1986-09-01 00:00:00

abstract：:Emotional signals are crucial for sharing important information, with conspecifics, for example, to warn humans of danger. Humans use a range of different cues to communicate to others how they feel, including facial, vocal, and gestural signals. We examined the recognition of nonverbal emotional vocalizations, such a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sauter DA,Eisner F,Ekman P,Scott SK

更新日期：2010-02-09 00:00:00

abstract：:The immunoglobulin heavy-chain-associated tetrapeptide, tuftsin (Thr-Lys-Pro-Arg), known for its phagocytosis-stimulating activity, was found to augment the antigen-specific, macrophage-dependent education of T lymphocytes. The investigation of stereospecific characteristics of the tetrapeptide, by use of structural a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tzehoval E,Segal S,Stabinsky Y,Fridkin M,Spirer Z,Feldman M

更新日期：1978-07-01 00:00:00

abstract：:It was shown in an earlier paper how to connect, in principle, the biochemical states of a cross-bridge with the mechanics of muscular contraction, by the methods of statistical mechanics. The treatment applies to cross-bridges that are able to interact with only one actin site at a time. The present paper shows that ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill TL

更新日期：1973-10-01 00:00:00

abstract：:We report here the crystal structure of the RuvB motor protein from Thermus thermophilus HB8, which drives branch migration of the Holliday junction during homologous recombination. RuvB has a crescent-like architecture consisting of three consecutive domains, the first two of which are involved in ATP binding and hyd...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada K,Kunishima N,Mayanagi K,Ohnishi T,Nishino T,Iwasaki H,Shinagawa H,Morikawa K

更新日期：2001-02-13 00:00:00

abstract：:The basis for the 200-fold difference in affinity between two hybridoma antibodies specific for the hapten p-azophenylarsonate (Ars) that have diversified by somatic hypermutation was examined. Oligonucleotide-directed mutagenesis was used to sequentially convert the nucleotide sequence of the lower-affinity antibody ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sharon J

更新日期：1990-06-01 00:00:00

abstract：:The recognition of carrier proteins by multiple catalytic partners occurs in every cycle of chain elongation in the biosynthesis of fatty acids and of the pharmacologically important polyketide and nonribosomal peptide natural products. To dissect the features of carrier proteins that determine specific recognition at...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou Z,Lai JR,Walsh CT

更新日期：2007-07-10 00:00:00

abstract：:Estrogen receptor beta (ER-beta) regulates diverse physiological functions in the human body. Current studies are confined to ER-beta1, and the functional roles of isoforms 2, 4, and 5 remain unclear. Full-length ER-beta4 and -beta5 isoforms were obtained from a prostate cell line, and they exhibit differential expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leung YK,Mak P,Hassan S,Ho SM

更新日期：2006-08-29 00:00:00

abstract：:Cells of the craniofacial skeleton are derived from a common mesenchymal progenitor. The regulatory factors that control their differentiation into various cell lineages are unknown. To investigate the biological function of dentin matrix protein 1 (DMP1), an extracellular matrix gene involved in calcified tissue form...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Narayanan K,Srinivas R,Ramachandran A,Hao J,Quinn B,George A

更新日期：2001-04-10 00:00:00

abstract：:Loss-of-function mutations in the regulatory gene areA of Aspergillus nidulans prevent the utilization of a wide variety of nitrogen sources. The phenotypes of nit-2 mutants of Neurospora crassa suggest that this gene may be analogous to the areA gene. Transformation has been used to introduce a plasmid containing the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Davis MA,Hynes MJ

更新日期：1987-06-01 00:00:00

abstract：:An association of the dopamine receptor D4 (DRD4) gene located on chromosome 11p15.5 and attention deficit/hyperactivity disorder (ADHD) has been demonstrated and replicated by multiple investigators. A specific allele [the 7-repeat of a 48-bp variable number of tandem repeats (VNTR) in exon 3] has been proposed as an...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swanson J,Oosterlaan J,Murias M,Schuck S,Flodman P,Spence MA,Wasdell M,Ding Y,Chi HC,Smith M,Mann M,Carlson C,Kennedy JL,Sergeant JA,Leung P,Zhang YP,Sadeh A,Chen C,Whalen CK,Babb KA,Moyzis R,Posner MI

更新日期：2000-04-25 00:00:00

abstract：:The mammalian AML/CBFalpha runt domain (RD) transcription factors regulate hematopoiesis and osteoblast differentiation. Like their Drosophila counterparts, most mammalian RD proteins terminate in a common pentapeptide, VWRPY, which serves to recruit the corepressor Groucho (Gro). Using a yeast two-hybrid assay, in vi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levanon D,Goldstein RE,Bernstein Y,Tang H,Goldenberg D,Stifani S,Paroush Z,Groner Y

更新日期：1998-09-29 00:00:00

abstract：:Homing endonucleases stimulate gene conversion by generating site-specific DNA double-strand breaks that are repaired by homologous recombination. These enzymes are potentially valuable tools for targeted gene correction and genome engineering. We have engineered a variant of the I-AniI homing endonuclease that nicks ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McConnell Smith A,Takeuchi R,Pellenz S,Davis L,Maizels N,Monnat RJ Jr,Stoddard BL

更新日期：2009-03-31 00:00:00