Abstract:
:Homing endonucleases stimulate gene conversion by generating site-specific DNA double-strand breaks that are repaired by homologous recombination. These enzymes are potentially valuable tools for targeted gene correction and genome engineering. We have engineered a variant of the I-AniI homing endonuclease that nicks its cognate target site. This variant contains a mutation of a basic residue essential for proton transfer and solvent activation in one active site. The cleavage mechanism, DNA-binding affinity, and substrate specificity profile of the nickase are similar to the wild-type enzyme. I-AniI nickase stimulates targeted gene correction in human cells, in cis and in trans, at approximately 1/4 the efficiency of the wild-type enzyme. The development of sequence-specific nicking enzymes like the I-AniI nickase will facilitate comparative analyses of DNA repair and mutagenesis induced by single- or double-strand breaks.
journal_name
Proc Natl Acad Sci U S Aauthors
McConnell Smith A,Takeuchi R,Pellenz S,Davis L,Maizels N,Monnat RJ Jr,Stoddard BLdoi
10.1073/pnas.0810588106subject
Has Abstractpub_date
2009-03-31 00:00:00pages
5099-104issue
13eissn
0027-8424issn
1091-6490pii
0810588106journal_volume
106pub_type
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