当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Creatine kinase: essential arginine residues at the nucleotide binding site identified by chemical modification and high-resolution tandem mass spectrometry.

Creatine kinase: essential arginine residues at the nucleotide binding site identified by chemical modification and high-resolution tandem mass spectrometry.

Abstract：

:Phenylglyoxal is an arginine-specific reagent that inactivates creatine kinase (CK). Previous results suggest that modification of the dimeric enzyme at a single arginine residue per subunit causes complete inactivation accompanied by the loss of nucleotide binding; the actual site of modification was not identified. Here, high-resolution tandem mass spectrometry (MS/MS) was used to identify three phenylglyoxal-modified Arg residues in monomeric rabbit muscle CK. Electrospray ionizaton Fourier-transform MS of the phenylglyoxal-modified CK that had lost approximately 80% activity identified three species: unmodified, once-modified (+116 Da), and twice-modified (+232 Da) enzyme in a ratio of approximately 1:4:1. MS/MS restricts the derivatized sites to P122-P212 and P283-V332, whereas MS of Lys-C digestions revealed two modified peptides, A266-K297 and G116-K137. The only Arg in A266-K297 is Arg-291 (invariant), whereas MS/MS of modified G116-K137 shows that two of the three sites Arg-129, Arg-131, or Arg-134 (all invariant) can contain the modification. The recently reported x-ray crystal structure for the octameric chicken mitochondrial CK indicates that its nucleotide triphosphate-binding site indeed contains the equivalent of R291, R129, and R131 reported here to be at the active site of rabbit muscle CK.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Wood TD,Guan Z,Borders CL Jr,Chen LH,Kenyon GL,McLafferty FW

doi

10.1073/pnas.95.7.3362

subject

Has Abstract

pub_date

1998-03-31 00:00:00

pages

3362-5

issue

7

eissn

0027-8424

issn

1091-6490

journal_volume

95

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Spliced segments at the 5' terminus of adenovirus 2 late mRNA.

    abstract：:An mRNA fraction coding for hexon polypeptide, the major virion structural protein, was purified by gel electrophoresis from extracts of adenovirus 2-infected cells late in the lytic cycle. The mRNA sequences in this fraction were mapped between 51.7 and 61.3 units on the genome by visualizing RNA-DNA hybrids in the e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.74.8.3171

    authors： Berget SM,Moore C,Sharp PA

    更新日期：1977-08-01 00:00:00

  • Identification of the yeast 20S proteasome catalytic centers and subunit interactions required for active-site formation.

    abstract：:The proteasome is responsible for degradation of substrates of the ubiquitin pathway. 20S proteasomes are cylindrical particles with subunits arranged in a stack of four heptameric rings. The outer rings are composed of alpha subunits, and the inner rings are composed of beta subunits. A well-characterized archaeal pr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.94.14.7156

    authors： Arendt CS,Hochstrasser M

    更新日期：1997-07-08 00:00:00

  • Theory for normal and impaired experience-dependent plasticity in neocortex of adult rats.

    abstract：:We model experience-dependent plasticity in the cortical representation of whiskers (the barrel cortex) in normal adult rats, and in adult rats that were prenatally exposed to alcohol. Prenatal exposure to alcohol (PAE) caused marked deficits in experience-dependent plasticity in a cortical barrel-column. Cortical pla...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.051346398

    authors： Benusková L,Rema V,Armstrong-James M,Ebner FF

    更新日期：2001-02-27 00:00:00

  • Translocation of synapsin I in response to depolarization of isolated nerve terminals.

    abstract：:Depolarization of isolated nerve terminals (synaptosomes) has been shown to stimulate neurotransmitter release and to increase the phosphorylation state of a number of proteins, including synapsin I, in a Ca2+-dependent manner. Synapsin I, a prominent nerve terminal phosphoprotein, interacts with the cytoplasmic surfa...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.20.8108

    authors： Sihra TS,Wang JK,Gorelick FS,Greengard P

    更新日期：1989-10-01 00:00:00

  • The wound repair response controls outcome to cutaneous leishmaniasis.

    abstract：:Chronic microbial infections are associated with fibrotic and inflammatory reactions known as granulomas showing similarities to wound-healing and tissue repair processes. We have previously mapped three leishmaniasis susceptibility loci, designated lmr1, -2, and -3, which exert their effect independently of T cell im...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0505630102

    authors： Sakthianandeswaren A,Elso CM,Simpson K,Curtis JM,Kumar B,Speed TP,Handman E,Foote SJ

    更新日期：2005-10-25 00:00:00

  • IL-15 in tumor microenvironment causes rejection of large established tumors by T cells in a noncognate T cell receptor-dependent manner.

    abstract：:A major challenge of cancer immunotherapy is the persistence and outgrowth of subpopulations that lose expression of the target antigen. IL-15 is a potent cytokine that can promote organ-specific autoimmunity when up-regulated on tissue cells. Here we report that T cells eradicated 2-wk-old solid tumors that expressed...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1301022110

    authors： Liu RB,Engels B,Schreiber K,Ciszewski C,Schietinger A,Schreiber H,Jabri B

    更新日期：2013-05-14 00:00:00

  • Three-dimensional structure of a protein from scorpion venom: a new structural class of neurotoxins.

    abstract：:The three-dimensional crystal structure of variant-3 toxin from the scorpion Centruroides sculpturatus Ewing has been determined at 3 A resolution. Phases were obtained by use of K2PtCl4 and K2IrCl6 derivatives. The most prominent secondary structural features are two and a half turns of alpha-helix and a three-strand...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.77.11.6496

    authors： Fontecilla-Camps JC,Almassy RJ,Suddath FL,Watt DD,Bugg CE

    更新日期：1980-11-01 00:00:00

  • In vitro synthesis of RNA that contains polyadenylate by virion-associated RNA polymerase of vesicular stomatitis virus.

    abstract：:The RNA synthesized in vitro by the virion-associated RNA-instructed RNA polymerase of purified vesicular stomatitis virus contains polyadenylate sequences. These have been demonstrated by their partial resistance to pancreatic and T(1) ribonucleases and their capacity to bind to poly(U) filters and oligo(dT)-cellulos...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.70.12.3566

    authors： Banerjee AK,Rhodes DP

    更新日期：1973-12-01 00:00:00

  • Inherent noise can facilitate coherence in collective swarm motion.

    abstract：:Among the most striking aspects of the movement of many animal groups are their sudden coherent changes in direction. Recent observations of locusts and starlings have shown that this directional switching is an intrinsic property of their motion. Similar direction switches are seen in self-propelled particle and othe...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0811195106

    authors： Yates CA,Erban R,Escudero C,Couzin ID,Buhl J,Kevrekidis IG,Maini PK,Sumpter DJ

    更新日期：2009-04-07 00:00:00

  • Conformational properties of the acetylcholine receptor as revealed by studies with constrained depolarizing ligands.

    abstract：:Conformational aspects of the acetylcholine receptor (AcChoR) of Electrophorus electricus have been examined by studies of its interaction with structurally related, constrained aromatic bis quaternary compounds. Among the compounds synthesized was 3,3'-bis[alpha-(trimethylammonium)-methyl]azobenzene dibromide (3,3'-b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.76.1.256

    authors： Wassermann NH,Bartels E,Erlanger BF

    更新日期：1979-01-01 00:00:00

  • Negative selection on human genes underlying inborn errors depends on disease outcome and both the mode and mechanism of inheritance.

    abstract：:Genetic variants underlying life-threatening diseases, being unlikely to be transmitted to the next generation, are gradually and selectively eliminated from the population through negative selection. We study the determinants of this evolutionary process in human genes underlying monogenic diseases by comparing vario...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2001248118

    authors： Rapaport F,Boisson B,Gregor A,Béziat V,Boisson-Dupuis S,Bustamante J,Jouanguy E,Puel A,Rosain J,Zhang Q,Zhang SY,Gleeson JG,Quintana-Murci L,Casanova JL,Abel L,Patin E

    更新日期：2021-01-19 00:00:00

  • Parental imprinting studied by allele-specific primer extension after PCR: paternal X chromosome-linked genes are transcribed prior to preferential paternal X chromosome inactivation.

    abstract：:The preferential inactivation of the paternal X chromosome in extraembryonic cells during early mouse development is an example of parental imprinting, but it has not been studied at the transcriptional level because standard methods of measuring RNA levels do not allow detection of allele-specific RNAs in individual ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.21.10469

    authors： Singer-Sam J,Chapman V,LeBon JM,Riggs AD

    更新日期：1992-11-01 00:00:00

  • Caspase 3 activation is essential for neuroprotection in preconditioning.

    abstract：:Sublethal insults can induce tolerance to subsequent stressors in neurons. As cell death activators such as ROS generation and decreased ATP can initiate tolerance, we tested whether other cellular elements normally associated with neuronal injury could add to this process. In an in vivo model of ischemic tolerance, w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0232966100

    authors： McLaughlin B,Hartnett KA,Erhardt JA,Legos JJ,White RF,Barone FC,Aizenman E

    更新日期：2003-01-21 00:00:00

  • Cellular and humoral immune responses to adenoviral vectors containing factor IX gene: tolerization of factor IX and vector antigens allows for long-term expression.

    abstract：:Recombinant adenoviruses containing the canine factor IX (FIX) cDNA were directly introduced in the hind leg muscle of mice. We show that (i) in nude mice, high expression (1-5 micrograms/ml in plasma) of FIX protein can be detected for > 300 days; (ii) in contrast, expression of FIX protein was transient (7-10 days) ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.5.1401

    authors： Dai Y,Schwarz EM,Gu D,Zhang WW,Sarvetnick N,Verma IM

    更新日期：1995-02-28 00:00:00

  • Tree-structured supervised learning and the genetics of hypertension.

    abstract：:This paper is about an algorithm, FlexTree, for general supervised learning. It extends the binary tree-structured approach (Classification and Regression Trees, CART) although it differs greatly in its selection and combination of predictors. It is particularly applicable to assessing interactions: gene by gene and g...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0403794101

    authors： Huang J,Lin A,Narasimhan B,Quertermous T,Hsiung CA,Ho LT,Grove JS,Olivier M,Ranade K,Risch NJ,Olshen RA

    更新日期：2004-07-20 00:00:00

  • Positive regulatory role of strigolactone in plant responses to drought and salt stress.

    abstract：:This report provides direct evidence that strigolactone (SL) positively regulates drought and high salinity responses in Arabidopsis. Both SL-deficient and SL-response [more axillary growth (max)] mutants exhibited hypersensitivity to drought and salt stress, which was associated with shoot- rather than root-related t...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1322135111

    authors： Ha CV,Leyva-González MA,Osakabe Y,Tran UT,Nishiyama R,Watanabe Y,Tanaka M,Seki M,Yamaguchi S,Dong NV,Yamaguchi-Shinozaki K,Shinozaki K,Herrera-Estrella L,Tran LS

    更新日期：2014-01-14 00:00:00

  • RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells.

    abstract：:Duplexes of 21-nt RNAs, known as short-interfering RNAs (siRNAs), efficiently inhibit gene expression by RNA interference (RNAi) when introduced into mammalian cells. We show that siRNAs can be synthesized by in vitro transcription with T7 RNA polymerase, providing an economical alternative to chemical synthesis of si...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.092143499

    authors： Yu JY,DeRuiter SL,Turner DL

    更新日期：2002-04-30 00:00:00

  • Two types of magnetic shape-memory effects from twinned microstructure and magneto-structural coupling in Fe1+y Te.

    abstract：:A detailed experimental investigation of Fe1+y Te (y = 0.11, 0.12) using pulsed magnetic fields up to 60 T confirms remarkable magnetic shape-memory (MSM) effects. These effects result from magnetoelastic transformation processes in the low-temperature antiferromagnetic state of these materials. The observation of mod...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1905271116

    authors： Rößler S,Koz C,Wang Z,Skourski Y,Doerr M,Kasinathan D,Rosner H,Schmidt M,Schwarz U,Rößler UK,Wirth S

    更新日期：2019-08-20 00:00:00

  • Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing.

    abstract：:In a search for Polo-like kinase 1 (Plk1)-interacting proteins using a yeast two-hybrid system, we have identified histone acetyltransferase binding to the origin recognition complex 1 (Hbo1) as a potential Plk1 target. Here, we show that the interaction between Plk1 and Hbo1 is mitosis-specific and that Plk1 phosphor...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0712063105

    authors： Wu ZQ,Liu X

    更新日期：2008-02-12 00:00:00

  • COX-2, a synaptically induced enzyme, is expressed by excitatory neurons at postsynaptic sites in rat cerebral cortex.

    abstract：:Postnatal development and adult function of the central nervous system are dependent on the capacity of neurons to effect long-term changes of specific properties in response to neural activity. This neuronal response has been demonstrated to be tightly correlated with the expression of a set of regulatory genes which...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.93.6.2317

    authors： Kaufmann WE,Worley PF,Pegg J,Bremer M,Isakson P

    更新日期：1996-03-19 00:00:00

  • Sparse production but preferential incorporation of recently produced naive T cells in the human peripheral pool.

    abstract：:In mice, recent thymic emigrants (RTEs) make up a large part of the naïve T cell pool and have been suggested to be a distinct short-lived pool. In humans, however, the life span and number of RTEs are unknown. Although (2)H(2)O labeling in young mice showed high thymic-dependent daily naïve T cell production, long te...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0709713105

    authors： Vrisekoop N,den Braber I,de Boer AB,Ruiter AF,Ackermans MT,van der Crabben SN,Schrijver EH,Spierenburg G,Sauerwein HP,Hazenberg MD,de Boer RJ,Miedema F,Borghans JA,Tesselaar K

    更新日期：2008-04-22 00:00:00

  • Defective antitermination of rRNA transcription and derepression of rRNA and tRNA synthesis in the nusB5 mutant of Escherichia coli.

    abstract：:The nusB5 mutant of Escherichia coli was originally selected for reduced ability to support the antitermination of transcription that is mediated by the gene N product of bacteriophage lambda. By analyzing pulse-labeled RNA with an RNA.DNA filter hybridization technique, we have shown that, in the nusB5 mutant, the ra...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.82.16.5275

    authors： Sharrock RA,Gourse RL,Nomura M

    更新日期：1985-08-01 00:00:00

  • Harm to others outweighs harm to self in moral decision making.

    abstract：:Concern for the suffering of others is central to moral decision making. How humans evaluate others' suffering, relative to their own suffering, is unknown. We investigated this question by inviting subjects to trade off profits for themselves against pain experienced either by themselves or an anonymous other person....

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章,随机对照试验

    doi：10.1073/pnas.1408988111

    authors： Crockett MJ,Kurth-Nelson Z,Siegel JZ,Dayan P,Dolan RJ

    更新日期：2014-12-02 00:00:00

  • Growth hormone (GH) induces tyrosine-phosphorylated proteins in mouse L cells that express recombinant GH receptors.

    abstract：:Porcine and bovine GH receptor (GHR) cDNAs were stably expressed in mouse L cells, which normally do not possess detectable levels of mouse GHR. Expression of the GHR cDNAs resulted in specific binding of 125I-labeled GH by these cell lines. To study GHR-related signaling events in these cells, protein tyrosine phosph...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.4.1391

    authors： Wang X,Xu B,Souza SC,Kopchick JJ

    更新日期：1994-02-15 00:00:00

  • Detecting conserved regulatory elements with the model genome of the Japanese puffer fish, Fugu rubripes.

    abstract：:Comparative vertebrate genome sequencing offers a powerful method for detecting conserved regulatory sequences. We propose that the compact genome of the teleost Fugu rubripes is well suited for this purpose. The evolutionary distance of teleosts from other vertebrates offers the maximum stringency for such evolutiona...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.92.5.1684

    authors： Aparicio S,Morrison A,Gould A,Gilthorpe J,Chaudhuri C,Rigby P,Krumlauf R,Brenner S

    更新日期：1995-02-28 00:00:00

  • A fluorescence-based assay for monitoring helicase activity.

    abstract：:A continuous fluorescence-based assay is described for measuring helicase-mediated unwinding of duplex DNA. The assay utilizes an oligonucleotide substrate containing the fluorescent adenine analog, 2-aminopurine, at regular intervals. 2-Aminopurine forms a Watson-Crick-type base pair with thymine and does not distort...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.14.6644

    authors： Raney KD,Sowers LC,Millar DP,Benkovic SJ

    更新日期：1994-07-05 00:00:00

  • Global view of the protein universe.

    abstract：:To explore protein space from a global perspective, we consider 9,710 SCOP (Structural Classification of Proteins) domains with up to 70% sequence identity and present all similarities among them as networks: In the "domain network," nodes represent domains, and edges connect domains that share "motifs," i.e., signifi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1403395111

    authors： Nepomnyachiy S,Ben-Tal N,Kolodny R

    更新日期：2014-08-12 00:00:00

  • Inhibition of spinal microglial cathepsin S for the reversal of neuropathic pain.

    abstract：:A recent major conceptual advance has been the recognition of the importance of immune system-neuronal interactions in the modulation of brain function, one example of which is spinal pain processing in neuropathic states. Here, we report that in peripheral nerve-injured rats, the lysosomal cysteine protease cathepsin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0610811104

    authors： Clark AK,Yip PK,Grist J,Gentry C,Staniland AA,Marchand F,Dehvari M,Wotherspoon G,Winter J,Ullah J,Bevan S,Malcangio M

    更新日期：2007-06-19 00:00:00

  • Statistical learning of visual transitions in monkey inferotemporal cortex.

    abstract：:One of the most fundamental functions of the brain is to predict upcoming events on the basis of the recent past. A closely related function is to signal when a prediction has been violated. The identity of the brain regions that mediate these functions is not known. We set out to determine whether they are implemente...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1112895108

    authors： Meyer T,Olson CR

    更新日期：2011-11-29 00:00:00

  • A dual laminin/collagen receptor acts in peripheral nerve regeneration.

    abstract：:A regeneration chamber was created in vivo by suturing a synthetic tube sealed at its distal end onto the proximal stump of a severed rat sciatic nerve. Nerves regenerated into tubes coated with laminin at a rate of 0.33 mm/day after a lag of about 2 days. At 25 days, regenerating nerves had extended 23% farther into ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.87.4.1319

    authors： Toyota B,Carbonetto S,David S

    更新日期：1990-02-01 00:00:00