当前位置： SCI文献检索 > PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA期刊下所有文献 > Identification of the yeast 20S proteasome catalytic centers and subunit interactions required for active-site formation.

Identification of the yeast 20S proteasome catalytic centers and subunit interactions required for active-site formation.

Abstract：

:The proteasome is responsible for degradation of substrates of the ubiquitin pathway. 20S proteasomes are cylindrical particles with subunits arranged in a stack of four heptameric rings. The outer rings are composed of alpha subunits, and the inner rings are composed of beta subunits. A well-characterized archaeal proteasome has a single type of each subunit, and the N-terminal threonine of the beta subunit is the active-site nucleophile. Yeast proteasomes have seven different beta subunits and exhibit several distinct peptidase activities, which were proposed to derive from disparate active sites. We show that mutating the N-terminal threonine in the yeast Pup1 beta subunit eliminates cleavage after basic residues in peptide substrates, and mutating the corresponding threonine of Pre3 prevents cleavage after acidic residues. Surprisingly, neither mutation has a strong effect on cell growth, and they have at most minor effects on ubiquitin-dependent proteolysis. We show that Pup1 interacts with Pup3 in each beta subunit ring. Our data reveal that different proteasome active sites contribute very differently to protein breakdown in vivo, that contacts between particular subunits in each beta subunit ring are critical for active-site formation, and that active sites in archaea and different eukaryotes are highly similar.

journal_name

Proc Natl Acad Sci U S A

journal_title

Proceedings of the National Academy of Sciences of the United States of America

authors

Arendt CS,Hochstrasser M

doi

10.1073/pnas.94.14.7156

subject

Has Abstract

pub_date

1997-07-08 00:00:00

pages

7156-61

issue

14

eissn

0027-8424

issn

1091-6490

journal_volume

94

pub_type

杂志文章

相关文献

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA文献大全
  • Identification and purification of an irreversible presynaptic neurotoxin from the venom of the spider Hololena curta.

    abstract：:A search for potent toxins that inhibit neuronal calcium channels in Drosophila melanogaster has resulted in the identification of a presynaptic neurotoxin from the venom of the hunting spider Hololena curta. Using Drosophila neuromuscular junction as an assay, presynaptic inhibitory activity was purified using gel fi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.10.3506

    authors： Bowers CW,Phillips HS,Lee P,Jan YN,Jan LY

    更新日期：1987-05-01 00:00:00

  • Core disgust is attenuated by ingroup relations.

    abstract：:We present the first experimental evidence to our knowledge that ingroup relations attenuate core disgust and that this helps explain the ability of groups to coact. In study 1, 45 student participants smelled a sweaty t-shirt bearing the logo of another university, with either their student identity (ingroup conditio...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1517027113

    authors： Reicher SD,Templeton A,Neville F,Ferrari L,Drury J

    更新日期：2016-03-08 00:00:00

  • Cardiomyocyte cyclooxygenase-2 influences cardiac rhythm and function.

    abstract：:Nonsteroidal anti-inflammatory drugs selective for inhibition of COX-2 increase heart failure and elevate blood pressure. The COX-2 gene was floxed and crossed into merCremer mice under the alpha-myosin heavy-chain promoter. Tamoxifen induced selective deletion of COX-2 in cardiomyocytes depressed cardiac output, and ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0805806106

    authors： Wang D,Patel VV,Ricciotti E,Zhou R,Levin MD,Gao E,Yu Z,Ferrari VA,Lu MM,Xu J,Zhang H,Hui Y,Cheng Y,Petrenko N,Yu Y,FitzGerald GA

    更新日期：2009-05-05 00:00:00

  • Antibodies in cerebrospinal fluid of some Alzheimer disease patients recognize cholinergic neurons in the rat central nervous system.

    abstract：:The etiology of Alzheimer disease is unclear. However, immunological aberrations have been suggested to be critical factors in the pathogenesis of this neurodegenerative disease. This study was carried out to investigate if cerebrospinal fluid (CSF) from Alzheimer disease patients contains antibodies that recognize sp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.84.24.9214

    authors： McRae-Degueurce A,Booj S,Haglid K,Rosengren L,Karlsson JE,Karlsson I,Wallin A,Svennerholm L,Gottfries CG,Dahlstrom A

    更新日期：1987-12-01 00:00:00

  • Polynucleotide phosphorylase functions both as a 3' right-arrow 5' exonuclease and a poly(A) polymerase in Escherichia coli.

    abstract：:In vitro, polynucleotide phosphorylase of Escherichia coli can both synthesize RNA by using nucleotide diphosphates as precursors and exonucleolytically degrade RNA in the presence of inorganic phosphate. However, because of the high in vivo concentration of inorganic phosphate in exponentially growing cells, it has b...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.220295997

    authors： Mohanty BK,Kushner SR

    更新日期：2000-10-24 00:00:00

  • Ingestion of Lactobacillus strain regulates emotional behavior and central GABA receptor expression in a mouse via the vagus nerve.

    abstract：:There is increasing, but largely indirect, evidence pointing to an effect of commensal gut microbiota on the central nervous system (CNS). However, it is unknown whether lactic acid bacteria such as Lactobacillus rhamnosus could have a direct effect on neurotransmitter receptors in the CNS in normal, healthy animals. ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1102999108

    authors： Bravo JA,Forsythe P,Chew MV,Escaravage E,Savignac HM,Dinan TG,Bienenstock J,Cryan JF

    更新日期：2011-09-20 00:00:00

  • Modification of microtubule steady-state dynamics by phosphorylation of the microtubule-associated proteins.

    abstract：:Phosphorylation of purified microtubule-associated proteins (MAPs) inhibits the rate and extent of MAP-stimulated microtubule assembly. The extent of microtubule assembly is reduced as a result of a decrease in the fraction of tubulin polymerized, without a significant change in the critical protein concentration. The...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.6.3413

    authors： Jameson L,Caplow M

    更新日期：1981-06-01 00:00:00

  • Secretion of human hepatitis B virus is inhibited by the imino sugar N-butyldeoxynojirimycin.

    abstract：:The imino sugar N-butyldeoxynojirimycin (NBDNJ) is a potent inhibitor of the oligosaccharide-trimming enzyme alpha-glucosidase I. Hepatitis B virus (HBV) contains three surface proteins (HBs proteins) of different sizes that are singly or doubly N-glycosylated and are essential for the formation of infectious virus. T...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.6.2235

    authors： Block TM,Lu X,Platt FM,Foster GR,Gerlich WH,Blumberg BS,Dwek RA

    更新日期：1994-03-15 00:00:00

  • Transgenic mouse model for human gastric carcinoma.

    abstract：:To understand the pathogenesis that may be induced by human adenovirus type 12 (Ad12), we have generated transgenic mice carrying the Ad12 early region 1 under control of the mouse mammary tumor virus long terminal repeat. Eleven of 11 male founder mice, but only 2 of 12 females, died between 3 to 4 mo of age. Death w...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.86.14.5615

    authors： Koike K,Hinrichs SH,Isselbacher KJ,Jay G

    更新日期：1989-07-01 00:00:00

  • Valproic acid interactions with the NavMs voltage-gated sodium channel.

    abstract：:Valproic acid (VPA) is an anticonvulsant drug that is also used to treat migraines and bipolar disorder. Its proposed biological targets include human voltage-gated sodium channels, among other membrane proteins. We used the prokaryotic NavMs sodium channel, which has been shown to be a good exemplar for drug binding ...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1909696116

    authors： Zanatta G,Sula A,Miles AJ,Ng LCT,Torella R,Pryde DC,DeCaen PG,Wallace BA

    更新日期：2019-12-10 00:00:00

  • Tumor-promoting phorbol esters stimulate myelopoiesis and suppress erythropoiesis in cultures of mouse bone marrow cells.

    abstract：:Tumor-promoting phorbol esters affect the ability of mouse hematopoietic progenitor cells to form morphologically recognizable colonies in culture. They induce myeloid progenitor cells to form colonies of the monocyte/macrophage type in the absence of exogenous granulocyte/macrophage colony-stimulating factor. Convers...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.78.7.4402

    authors： Sieber F,Stuart RK,Spivak JL

    更新日期：1981-07-01 00:00:00

  • Long-term retention of learning-induced receptive-field plasticity in the auditory cortex.

    abstract：:Brief learning experience (classical conditioning) induces frequency-specific receptive-field (RF) plasticity in the auditory cortex, characterized as increased response to the frequency of the conditioned stimulus and decreased responses to most other frequencies, including the pretraining best frequency. This experi...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.90.6.2394

    authors： Weinberger NM,Javid R,Lepan B

    更新日期：1993-03-15 00:00:00

  • Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.

    abstract：:Molecular weights of the isolated proteins from the 30S and 50S ribosomal subunits of Escherichia coli were determined by two independent methods: polyacrylamide gel electrophoresis with sodium dodecylsulphate and equilibrium sedimentation. The values for the molecular weights determined by gel electrophoresis range f...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.67.4.1909

    authors： Dzionara M,Kaltschmidt E,Wittmann HG

    更新日期：1970-12-01 00:00:00

  • A cytochrome b5 is required for full activity of flavonoid 3', 5'-hydroxylase, a cytochrome P450 involved in the formation of blue flower colors.

    abstract：:The substitution pattern of anthocyanin pigments is a main determinant of flower color. Flavonoid 3',5'-hydroxylase (F3'5'H) is a cytochrome P450 enzyme (Cyt P450) that catalyzes the 3', 5'-hydroxylation of dihydroflavonols, the precursors of purple anthocyanins. Species such as rose and carnation lack F3'5'H activity...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.96.2.778

    authors： de Vetten N,ter Horst J,van Schaik HP,de Boer A,Mol J,Koes R

    更新日期：1999-01-19 00:00:00

  • Chemotaxis of sperm cells.

    abstract：:We develop a theoretical description of sperm chemotaxis. Sperm cells of many species are guided to the egg by chemoattractants, a process called chemotaxis. Motor proteins in the flagellum of the sperm generate a regular beat of the flagellum, which propels the sperm in a fluid. In the absence of a chemoattractant, s...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0703530104

    authors： Friedrich BM,Jülicher F

    更新日期：2007-08-14 00:00:00

  • Superantigenic character of an insert unique to SARS-CoV-2 spike supported by skewed TCR repertoire in patients with hyperinflammation.

    abstract：:Multisystem Inflammatory Syndrome in Children (MIS-C) associated with COVID-19 is a newly recognized condition in children with recent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. These children and adult patients with severe hyperinflammation present with a constellation of symptoms that st...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.2010722117

    authors： Cheng MH,Zhang S,Porritt RA,Noval Rivas M,Paschold L,Willscher E,Binder M,Arditi M,Bahar I

    更新日期：2020-10-13 00:00:00

  • Brief visual experience induces immediate early gene expression in the cat visual cortex.

    abstract：:Brief visual experience causes rapid physiological changes in the visual cortex during early postnatal development. A possible mediator of these effects is the immediate early genes whose protein products are involved in the rapid response of neurons to transsynaptic stimulation. Here we report evidence that the level...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.12.5437

    authors： Rosen KM,McCormack MA,Villa-Komaroff L,Mower GD

    更新日期：1992-06-15 00:00:00

  • Recurrent mismatch binding by MutS mobile clamps on DNA localizes repair complexes nearby.

    abstract：:DNA mismatch repair (MMR), the guardian of the genome, commences when MutS identifies a mismatch and recruits MutL to nick the error-containing strand, allowing excision and DNA resynthesis. Dominant MMR models posit that after mismatch recognition, ATP converts MutS to a hydrolysis-independent, diffusive mobile clamp...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1918517117

    authors： Hao P,LeBlanc SJ,Case BC,Elston TC,Hingorani MM,Erie DA,Weninger KR

    更新日期：2020-07-28 00:00:00

  • Propensity for a leucine zipper-like domain of human immunodeficiency virus type 1 gp41 to form oligomers correlates with a role in virus-induced fusion rather than assembly of the glycoprotein complex.

    abstract：:For a number of viruses, oligomerization is a critical component of envelope processing and surface expression. Previously, we reported that a synthetic peptide (DP-107) corresponding to the putative leucine zipper region (aa 553-590) of the transmembrane protein (gp41) of human immunodeficiency virus type 1 (HIV-1) e...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.91.26.12676

    authors： Wild C,Dubay JW,Greenwell T,Baird T Jr,Oas TG,McDanal C,Hunter E,Matthews T

    更新日期：1994-12-20 00:00:00

  • In vivo imaging of calcium accumulation in fly interneurons as elicited by visual motion stimulation.

    abstract：:The computation of motion plays a central role in visual orientation. The fly has been successfully used as a model system for analyzing the mechanisms underlying motion detection. Thereby, much attention has been paid to a neuronal circuit of individually identifiable neurons in the third visual ganglion that extract...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.9.4139

    authors： Borst A,Egelhaaf M

    更新日期：1992-05-01 00:00:00

  • Nuclear resonance vibrational spectroscopic and computational study of high-valent diiron complexes relevant to enzyme intermediates.

    abstract：:High-valent intermediates of binuclear nonheme iron enzymes are structurally unknown despite their importance for understanding enzyme reactivity. Nuclear resonance vibrational spectroscopy combined with density functional theory calculations has been applied to structurally well-characterized high-valent mono- and di...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1304238110

    authors： Park K,Bell CB 3rd,Liu LV,Wang D,Xue G,Kwak Y,Wong SD,Light KM,Zhao J,Alp EE,Yoda Y,Saito M,Kobayashi Y,Ohta T,Seto M,Que L Jr,Solomon EI

    更新日期：2013-04-16 00:00:00

  • Molecular dioxygen enters the active site of 12/15-lipoxygenase via dynamic oxygen access channels.

    abstract：:Cells contain numerous enzymes that use molecular oxygen for their reactions. Often, their active sites are buried deeply inside the protein, which raises the question whether there are specific access channels guiding oxygen to the site of catalysis. Choosing 12/15-lipoxygenase as a typical example for such oxygen-de...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0702401104

    authors： Saam J,Ivanov I,Walther M,Holzhütter HG,Kuhn H

    更新日期：2007-08-14 00:00:00

  • Measuring single-cell density.

    abstract：:We have used a microfluidic mass sensor to measure the density of single living cells. By weighing each cell in two fluids of different densities, our technique measures the single-cell mass, volume, and density of approximately 500 cells per hour with a density precision of 0.001 g mL(-1). We observe that the intrins...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1104651108

    authors： Grover WH,Bryan AK,Diez-Silva M,Suresh S,Higgins JM,Manalis SR

    更新日期：2011-07-05 00:00:00

  • Adversarial alignment enables competing models to engage in cooperative theory building toward cumulative science.

    abstract：:Crises in science concern not only methods, statistics, and results but also, theory development. Beyond the indispensable refinement of tools and procedures, resolving crises would also benefit from a deeper understanding of the concepts and processes guiding research. Usually, theories compete, and some lose, incent...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1906720117

    authors： Ellemers N,Fiske ST,Abele AE,Koch A,Yzerbyt V

    更新日期：2020-04-07 00:00:00

  • An essential role for RPE-derived soluble VEGF in the maintenance of the choriocapillaris.

    abstract：:Clinical and experimental observations indicate a role for VEGF secreted by the retinal pigment epithelium (RPE) in the maintenance of the choriocapillaris (CC). VEGF in mice is produced as three isoforms, VEGF120, VEGF164, and VEGF188, that differ in their ability to bind heparan sulfate proteoglycan. RPE normally pr...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0905010106

    authors： Saint-Geniez M,Kurihara T,Sekiyama E,Maldonado AE,D'Amore PA

    更新日期：2009-11-03 00:00:00

  • Metallacycle-cored supramolecular assemblies with tunable fluorescence including white-light emission.

    abstract：:Control over the fluorescence of supramolecular assemblies is crucial for the development of chemosensors and light-emitting materials. Consequently, the postsynthetic modification of supramolecular structures via host-guest interactions has emerged as an efficient strategy in recent years that allows the facile tunin...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1702510114

    authors： Zhang M,Yin S,Zhang J,Zhou Z,Saha ML,Lu C,Stang PJ

    更新日期：2017-03-21 00:00:00

  • Estimation of evolutionary distances under stationary and nonstationary models of nucleotide substitution.

    abstract：:Estimation of evolutionary distances has always been a major issue in the study of molecular evolution because evolutionary distances are required for estimating the rate of evolution in a gene, the divergence dates between genes or organisms, and the relationships among genes or organisms. Other closely related issue...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.95.11.5899

    authors： Gu X,Li WH

    更新日期：1998-05-26 00:00:00

  • Practical zero-shift tuning in geonium.

    abstract：:Compositeness of the electron may show up in a very small deviation of the measured electron g factor from one calculated for a point electron by quantum electrodynamics. The precision of our g measurements is currently limited by an interaction of the cyclotron motion with standing waves in the trap cavity containing...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.89.5.1681

    authors： Dehmelt H,Van Dyck R Jr,Palmer F

    更新日期：1992-03-01 00:00:00

  • Microbial biospherics: The experimental study of ecosystem function and evolution.

    abstract：:Awareness that our planet is a self-supporting biosphere with sunlight as its major source of energy for life has resulted in a long-term historical fascination with the workings of self-supporting ecological systems. However, the studies of such systems have never entered the canon of ecological or evolutionary tools...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.1904326116

    authors： Rillig MC,Antonovics J

    更新日期：2019-06-04 00:00:00

  • The identification of an osteoclastogenesis inhibitor through the inhibition of glyoxalase I.

    abstract：:Osteoclasts, bone-resorptive multinucleated cells derived from hematopoietic stem cells, are associated with many bone-related diseases, such as osteoporosis. Osteoclast-targeting small-molecule inhibitors are valuable tools for studying osteoclast biology and for developing antiresorptive agents. Here, we have discov...

    journal_title：Proceedings of the National Academy of Sciences of the United States of America

    pub_type： 杂志文章

    doi：10.1073/pnas.0712239105

    authors： Kawatani M,Okumura H,Honda K,Kanoh N,Muroi M,Dohmae N,Takami M,Kitagawa M,Futamura Y,Imoto M,Osada H

    更新日期：2008-08-19 00:00:00