In vitro synthesis of RNA that contains polyadenylate by virion-associated RNA polymerase of vesicular stomatitis virus.

abstract：:In Escherichia coli, AK I- HDH I and AK II- HDH II are two bifunctional proteins, derived from a common ancestor, that catalyze the first and third reactions of the common pathway leading to threonine and methionine. An extensive amino acid sequence comparison of both molecules reveals two main features on each of the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ferrara P,Duchange N,Zakin MM,Cohen GN

更新日期：1984-05-01 00:00:00

abstract：:We have screened a human DNA library using as probe a chimpanzee sequence that contains homology to the polymerase gene of the endogenous baboon virus. One set of overlapping clones spans about 20 kilobases and contains regions of DNA sequence homology to the gag p30, gag p15, and polymerase genes of Moloney murine le...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonner TI,O'Connell C,Cohen M

更新日期：1982-08-01 00:00:00

abstract：:The completion of the human genome project and the development of high-throughput approaches herald a dramatic acceleration in the pace of biological research. One of the most compelling next steps will be learning the functional roles of all proteins. Achievement of this goal depends in part on the rapid expression a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braun P,Hu Y,Shen B,Halleck A,Koundinya M,Harlow E,LaBaer J

更新日期：2002-03-05 00:00:00

abstract：:It is shown that a stereochemical correspondence between two molecular systems can be represented by a commuting diagram of the point groups and permutation groups involved. The effect of the diagrammatic condition on the mappings of the cosets, double cosets, subgroup lattice, and double coset algebra determined for ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nourse JG

更新日期：1975-06-01 00:00:00

abstract：:During vertebrate cytokinesis it is thought that contractile ring constriction is driven by nonmuscle myosin II (NM II) translocation of antiparallel actin filaments. Here we report in situ, in vitro, and in vivo observations that challenge this hypothesis. Graded knockdown of NM II in cultured COS-7 cells reveals tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ma X,Kovács M,Conti MA,Wang A,Zhang Y,Sellers JR,Adelstein RS

更新日期：2012-03-20 00:00:00

abstract：:The molecular size and stability of the nerve growth factor (NGF) secreted in culture by L cells have been studied by sedimentation and gel filtration chromatography. Results indicate that L cell NGF has a molecular weight of 160,000. It contains as part of its structure a protein component that is biologically, immun...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pantazis NJ,Blanchard MH,Arnason BG,Young M

更新日期：1977-04-01 00:00:00

abstract：:We provide evidence for an interaction between mouse class I major histocompatibility complex antigens and insulin receptors. Antibodies against class I but not class II major histocompatibility complex antigens immunoprecipitate photoaffinity-labeled hepatic insulin receptors. Haplotype specificity is demonstrated by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Phillips ML,Moule ML,Delovitch TL,Yip CC

更新日期：1986-05-01 00:00:00

abstract：:HD domain phosphohydrolase enzymes are characterized by a conserved set of histidine and aspartate residues that coordinate an active site metallocenter. Despite the important roles these enzymes play in nucleotide metabolism and signal transduction, few have been both biochemically and structurally characterized. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bridwell-Rabb J,Kang G,Zhong A,Liu HW,Drennan CL

更新日期：2016-11-29 00:00:00

abstract：:The biological function of Glu-181 in the photoactivation process of rhodopsin is explored through spectroscopic studies of site-specific mutants. Preresonance Raman vibrational spectra of the unphotolyzed E181Q mutant are nearly identical to spectra of the native pigment, supporting the view that Glu-181 is uncharged...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yan EC,Kazmi MA,Ganim Z,Hou JM,Pan D,Chang BS,Sakmar TP,Mathies RA

更新日期：2003-08-05 00:00:00

abstract：:Two pools of heparan sulfate proteoglycans have been selectively solubilized from rat liver plasma membranes by successive incubations with heparin and detergent. The two populations of proteoglycans have similar polyanionic properties as indicated by identical elution positions on ion-exchange chromatography on DEAE-...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kjellén L,Pettersson I,Höök M

更新日期：1981-09-01 00:00:00

abstract：:We have constructed and characterized a polyoma virus-based plasmid that is maintained as an autonomously replicating extrachromosomal element (episome) in mouse embryonic stem (ES) cells. Plasmid pMGD20neo contains the polyoma origin of replication harboring a mutated enhancer (PyF101), a modified polyoma early regio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gassmann M,Donoho G,Berg P

更新日期：1995-02-28 00:00:00

abstract：:The chemical speciation of arsenic in sediments and porewaters of aquifers is the critical factor that determines whether dissolved arsenic accumulates to potentially toxic levels. Sequestration of arsenic in solid phases, which may occur by adsorption or precipitation processes, controls dissolved concentrations. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Day PA,Vlassopoulos D,Root R,Rivera N

更新日期：2004-09-21 00:00:00

abstract：:Previous studies have shown that DNA methyltransferase (Dnmt) 1 is required for maintenance of bulk DNA methylation and is essential for mouse development. However, somatic disruption of DNMT1 in the human cancer cell line HCT116 was not lethal and caused only minor decreases in methylation. Here, we report the identi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Egger G,Jeong S,Escobar SG,Cortez CC,Li TW,Saito Y,Yoo CB,Jones PA,Liang G

更新日期：2006-09-19 00:00:00

abstract：:Four mouse POU domain genomic DNA clones--Brain-1, Brain-2, Brain-4, and Scip--and Brain-2 cDNA, which are expressed in adult brain, were cloned and the coding and noncoding regions of the genes were sequenced. The amino acid sequences of the four POU domains are highly conserved; sequences in other regions of the pro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hara Y,Rovescalli AC,Kim Y,Nirenberg M

更新日期：1992-04-15 00:00:00

abstract：:Opioid receptors are differentially coupled to ion channels. Mu- and delta-opioid receptors are coupled to calcium- and/or voltage-dependent potassium channels and kappa-opioid receptors are coupled to voltage-dependent calcium channels. Using the single-electrode voltage-clamp technique, we investigated the effect of...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gross RA,Macdonald RL

更新日期：1987-08-01 00:00:00

abstract：:We have cloned one of the two human alpha-globin genes and report its complete nucleotide sequence. The gene is 832 base pairs (bp) long from the 5'-cap site to the 3'-polyadenylylation site. The amino acid coding sequences are separated into three segments (exons) by two short (117 and 140 bp) intervening sequences. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liebhaber SA,Goossens MJ,Kan YW

更新日期：1980-12-01 00:00:00

abstract：:Cones show briefer light responses than rods and do not saturate even under very bright light. Using purified rod and cone homogenates, we measured the activity of guanylate cyclase (GC), an enzyme responsible for cGMP synthesis and therefore recovery of a light response. The basal GC activity was 36 times higher in c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Takemoto N,Tachibanaki S,Kawamura S

更新日期：2009-07-14 00:00:00

abstract：:High-volume hydraulic fracturing (HVHF) has revolutionized the oil and gas industry worldwide but has been accompanied by highly controversial incidents of reported water contamination. For example, groundwater contamination by stray natural gas and spillage of brine and other gas drilling-related fluids is known to o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Llewellyn GT,Dorman F,Westland JL,Yoxtheimer D,Grieve P,Sowers T,Humston-Fulmer E,Brantley SL

更新日期：2015-05-19 00:00:00

abstract：:We describe a protein family in Drosophila containing six adenosine deaminase-related growth factors (ADGFs), which are homologous to a mitogenic growth factor discovered in conditioned medium from cells of a different fly species, Sarcophaga. Closely related proteins have been identified in other animals, and a human...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zurovec M,Dolezal T,Gazi M,Pavlova E,Bryant PJ

更新日期：2002-04-02 00:00:00

abstract：:A library of amphiphilic Janus dendrimers including two that are fluorescent and one glycodendrimer presenting lactose were used to construct giant dendrimersomes and glycodendrimersomes. Coassembly with the components of bacterial membrane vesicles by a dehydration-rehydration process generated giant cell-like hybrid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xiao Q,Yadavalli SS,Zhang S,Sherman SE,Fiorin E,da Silva L,Wilson DA,Hammer DA,André S,Gabius HJ,Klein ML,Goulian M,Percec V

更新日期：2016-03-01 00:00:00

abstract：:The essential eukaryotic pre-mRNA splicing factor U2AF (U2 small nuclear ribonucleoprotein auxiliary factor) is required to specify the 3' splice at an early step in spliceosome assembly. U2AF binds site-specifically to the intron polypyrimidine tract and recruits U2 small nuclear ribonucleoprotein to the branch site....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rudner DZ,Kanaar R,Breger KS,Rio DC

更新日期：1996-09-17 00:00:00

abstract：:The human genome is made up of long DNA segments, the isochores, which are compositionally homogeneous and can be subdivided into a small number of families characterized by different G+C levels. Chromosome in situ suppression hybridization (in which excess unlabeled human DNA is added to suppress hybridization of rep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Saccone S,De Sario A,Wiegant J,Raap AK,Della Valle G,Bernardi G

更新日期：1993-12-15 00:00:00

abstract：:Atherosclerosis is a complex disease resulting from the interaction of multiple genes. We have used the Ldlr knockout mouse model in an interspecific genetic cross to map atherosclerosis susceptibility loci. A total of 174 (MOLF/Ei x B6.129S7-Ldlr(tm1Her)) x C57BL/6J-Ldlr(tm1Her) backcross mice, homozygous for the Ldl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Welch CL,Bretschger S,Latib N,Bezouevski M,Guo Y,Pleskac N,Liang CP,Barlow C,Dansky H,Breslow JL,Tall AR

更新日期：2001-07-03 00:00:00

abstract：:Calcific aortic valve disease is a chronic inflammatory process, and aortic valve interstitial cells (AVICs) from diseased aortic valves express greater levels of osteogenic factors in response to proinflammatory stimulation. Here, we report that lower cellular levels of IL-37 in AVICs of diseased human aortic valves ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zeng Q,Song R,Fullerton DA,Ao L,Zhai Y,Li S,Ballak DB,Cleveland JC Jr,Reece TB,McKinsey TA,Xu D,Dinarello CA,Meng X

更新日期：2017-02-14 00:00:00

abstract：:Activation of eukaryotic class II gene expression involves the formation of a transcription initiation complex that includes RNA polymerase II, general transcription factors, and SRB components of the holoenzyme. Negative regulators of transcription have been described, but it is not clear whether any are general repr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gadbois EL,Chao DM,Reese JC,Green MR,Young RA

更新日期：1997-04-01 00:00:00

abstract：:The peptide composition of purified Histocompatibility-2 (H-2) reactive glycoprotein fragments from two murine strains allelic at the H-2 locus (H-2(b) and H-2(d)) were compared by a cellulose thin-layer peptide mapping micro technique. Approximately 70 per cent of the theoretical maximum number of peptides produced b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shimada A,Yamane K,Nathenson SG

更新日期：1970-03-01 00:00:00

abstract：:In everyday life, a macroscopic valve is a device with a movable control element that regulates the flow of gases or liquids by blocking and opening passageways. Construction of such a device on the nanoscale level requires (i) suitably proportioned movable control elements, (ii) a method for operating them on demand,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nguyen TD,Tseng HR,Celestre PC,Flood AH,Liu Y,Stoddart JF,Zink JI

更新日期：2005-07-19 00:00:00

abstract：:Examples of finite-dimensional Hopf algebras over a field, whose antipodes have arbitrary even orders >/=4 as mappings, are furnished. The dimension of the Hopf algebra is q(n+1), where the antipode has order 2q, q >/= 2, and n is an arbitrary positive integer. The algebras are not semisimple, and neither they nor the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Taft EJ

更新日期：1971-11-01 00:00:00

abstract：:An antiserum was prepared in rabbits against an antigen obtained by density gradient sedimentation of centrifuged medium from monolayer cultures of spleens involved by Hodgkin's disease. The antiserum was tested by isotopic antibody techniques with cells from each of eight cultures derived from spleens involved by Hod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Long JC,Aisenberg AC,Zamecnik PC

更新日期：1974-07-01 00:00:00

abstract：:Studies of the accelerated aging disorder Hutchinson-Gilford progeria syndrome (HGPS) can potentially reveal cellular defects associated with physiological aging. HGPS results from expression and abnormal nuclear envelope association of a farnesylated, truncated variant of prelamin A called "progerin." We surveyed the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang W,Wang Y,Luxton GWG,Östlund C,Worman HJ,Gundersen GG

更新日期：2019-02-26 00:00:00