Identification of DNMT1 (DNA methyltransferase 1) hypomorphs in somatic knockouts suggests an essential role for DNMT1 in cell survival.

abstract：:Computational and biochemical studies implicate the blue-light sensor cryptochrome (CRY) as an endogenous light-dependent magnetosensor enabling migratory birds to navigate using the Earth's magnetic field. Validation of such a mechanism has been hampered by the absence of structures of vertebrate CRYs that have funct...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zoltowski BD,Chelliah Y,Wickramaratne A,Jarocha L,Karki N,Xu W,Mouritsen H,Hore PJ,Hibbs RE,Green CB,Takahashi JS

更新日期：2019-09-24 00:00:00

abstract：:The enterotoxigenic Escherichia coli (ETEC) are among the most common causes of diarrheal illness and death due to diarrhea among young children in low-/middle-income countries (LMICs). ETEC have also been associated with important sequelae including malnutrition and stunting, placing children at further risk of death...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sheikh A,Tumala B,Vickers TJ,Alvarado D,Ciorba MA,Bhuiyan TR,Qadri F,Singer BB,Fleckenstein JM

更新日期：2020-11-17 00:00:00

abstract：:Volumetric detection and accurate quantification of fluorescent proteins in entire animals would greatly enhance our ability to monitor biological processes in vivo. Here we present a quantitative tomographic technique for visualization of superficial and deep-seated (>2-3 mm) fluorescent protein activity in vivo. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zacharakis G,Kambara H,Shih H,Ripoll J,Grimm J,Saeki Y,Weissleder R,Ntziachristos V

更新日期：2005-12-20 00:00:00

abstract：:Thermal macrophysiology is an established research field that has led to well-described patterns in the global structuring of climate adaptation and risk. However, since it was developed primarily in animals, we lack information on how general these patterns are across organisms. This is alarming if we are to understa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lancaster LT,Humphreys AM

更新日期：2020-06-16 00:00:00

abstract：:Oxidative stress plays a central role in many human diseases and in aging. In Caenorhabditis elegans the SKN-1 protein induces phase II detoxification gene transcription, a conserved oxidative stress response, and is required for oxidative stress resistance and longevity. Oxidative stress induces SKN-1 to accumulate i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： An JH,Vranas K,Lucke M,Inoue H,Hisamoto N,Matsumoto K,Blackwell TK

更新日期：2005-11-08 00:00:00

abstract：:Fas, a member of the tumor necrosis factor receptor family, can induce apoptosis when activated by Fas ligand binding or anti-Fas antibody crosslinking. Genetic studies have shown that a defect in Fas-mediated apoptosis resulted in abnormal development and function of the immune system in mice. A point mutation in the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chu K,Niu X,Williams LT

更新日期：1995-12-05 00:00:00

abstract：:The rates of spontaneous mutation to 6-thioguanine resistance were determined in fibroblasts derived from normal and two Bloom syndrome individuals (GM 2548 and GM 1492). Two methods were utilized to determine the rates. Method I obtained the spontaneous mutation rate from the increase in the mutation frequency of a c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warren ST,Schultz RA,Chang CC,Wade MH,Trosko JE

更新日期：1981-05-01 00:00:00

abstract：:Mechanical injury to the adult mammalian spinal cord results in permanent loss of structural integrity at the lesion site and of the brain-controlled function distal to the lesion. Some of these consequences were permanently averted by altering the cellular constituents at the lesion site with x-irradiation delivered ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kalderon N,Fuks Z

更新日期：1996-10-01 00:00:00

abstract：:Mouse serosal mast cells (SMCs) and Kirsten sarcoma virus-immortalized mast cells store large amounts of mast cell carboxypeptidase A and serine proteases in their secretory granules. Secretory granule proteins from 2.6 x 10(6) purified SMCs were separated by NaDodSO4/PAGE, trans-blotted to poly(vinylidine difluoride)...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds DS,Stevens RL,Lane WS,Carr MH,Austen KF,Serafin WE

更新日期：1990-04-01 00:00:00

abstract：:The HIV envelope (Env) protein mediates entry into cells by binding CD4 and an appropriate coreceptor, which triggers structural changes in Env that lead to fusion between the viral and cellular membranes. The major HIV-1 coreceptors are the seven transmembrane domain chemokine receptors CCR5 and CXCR4. The type of co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hoffman TL,Canziani G,Jia L,Rucker J,Doms RW

更新日期：2000-10-10 00:00:00

abstract：:The inhibitory neurotransmitter gamma-aminobutyric acid (GABA) is involved in the generation of various brain rhythmic activities that can be modulated by benzodiazepines. Here, we assessed the contribution of alpha(2)GABA type A (GABA(A)) receptors to the effects of benzodiazepines on sleep and waking oscillatory pat...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kopp C,Rudolph U,Löw K,Tobler I

更新日期：2004-03-09 00:00:00

abstract：:The size of virus-specific RNA synthesized in cultured mouse kidney cells infected with polyoma virus was estimated by electrophoresis and sedimentation analysis of RNA extracts from whole cells. Newly synthesized "late" polyoma-specific RNA appears as "giant" molecules of heterogeneous size, up to several times large...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Acheson NH,Buetti E,Scherrer K,Weil R

更新日期：1971-09-01 00:00:00

abstract：:The relatively short read lengths associated with the most cost-effective DNA sequencing technologies have limited their use in de novo genome assembly, structural variation detection, and haplotype-resolved genome sequencing. Consequently, there is a strong need for methods that capture various scales of contiguity i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schwartz JJ,Lee C,Hiatt JB,Adey A,Shendure J

更新日期：2012-11-13 00:00:00

abstract：:A variety of agricultural plant species, including corn, respond to insect herbivore damage by releasing large quantities of volatile compounds and, as a result, become highly attractive to parasitic wasps that attack the herbivores. An elicitor of plant volatiles, N-(17-hydroxylinolenoyl)-L-glutamine, named volicitin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Paré PW,Alborn HT,Tumlinson JH

更新日期：1998-11-10 00:00:00

abstract：:We identified previously in vitro LRP4 (low-density lipoprotein receptor-related protein 4) as a facilitator of the WNT (Wingless-type) antagonist sclerostin and found mutations disrupting this function to be associated with high bone mass in humans similar to patients lacking sclerostin. To further delineate the role...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang MK,Kramer I,Huber T,Kinzel B,Guth-Gundel S,Leupin O,Kneissel M

更新日期：2014-12-02 00:00:00

abstract：:We report the complete sequence of an extreme halophile, Halobacterium sp. NRC-1, harboring a dynamic 2,571,010-bp genome containing 91 insertion sequences representing 12 families and organized into a large chromosome and 2 related minichromosomes. The Halobacterium NRC-1 genome codes for 2,630 predicted proteins, 36...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ng WV,Kennedy SP,Mahairas GG,Berquist B,Pan M,Shukla HD,Lasky SR,Baliga NS,Thorsson V,Sbrogna J,Swartzell S,Weir D,Hall J,Dahl TA,Welti R,Goo YA,Leithauser B,Keller K,Cruz R,Danson MJ,Hough DW,Maddocks DG,Jabl

更新日期：2000-10-24 00:00:00

abstract：:L-Gulono-gamma-lactone oxidase (L-gulono-gamma-lactone:oxygen 2-oxidoreductase, EC 1.1.3.8) is the enzyme that catalyzes the terminal step of L-ascorbic acid biosynthesis in mammalian liver. The absence of the oxidase activity in primates and guinea pigs is the reason why these animals are subject to scurvy, which mus...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nishikimi M,Udenfriend S

更新日期：1976-06-01 00:00:00

abstract：:The development of distinct regions in the amniote vertebral column results from somite formation and Hox gene expression, with the adult morphology displaying remarkable variation among lineages. Mammalian regionalization is reportedly very conservative or even constrained, but there has been no study investigating v...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Müller J,Scheyer TM,Head JJ,Barrett PM,Werneburg I,Ericson PG,Pol D,Sánchez-Villagra MR

更新日期：2010-02-02 00:00:00

abstract：:Ca(2+) signaling regulates cell function. This is subject to modulation by H(+) ions that are universal end-products of metabolism. Due to slow diffusion and common buffers, changes in cytoplasmic [Ca(2+)] ([Ca(2+)]i) or [H(+)] ([H(+)]i) can become compartmentalized, leading potentially to complex spatial Ca(2+)/H(+) ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Swietach P,Youm JB,Saegusa N,Leem CH,Spitzer KW,Vaughan-Jones RD

更新日期：2013-05-28 00:00:00

abstract：:The x-ray crystal structure of an oligomeric miniprotein has been determined to a 1.2-A resolution by means of multiwavelength anomalous diffraction phasing with selenomethionine analogs that retain the biophysical characteristics of the native peptide. Peptide 1, comprising alpha and beta secondary structure elements...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ali MH,Peisach E,Allen KN,Imperiali B

更新日期：2004-08-17 00:00:00

abstract：:The synaptic vesicle membrane protein synaptotagmin (tagmin) is essential for fast, calcium-dependent, neurotransmitter release and is likely to be the calcium sensor for exocytosis, because of its many calcium-dependent properties. Polyphosphoinositides are needed for exocytosis, but it has not been known why. We now...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schiavo G,Gu QM,Prestwich GD,Söllner TH,Rothman JE

更新日期：1996-11-12 00:00:00

abstract：:In this paper I review the ways in which the glassy state is obtained both in nature and in materials science and highlight a "new twist"--the recent recognition of polymorphism within the glassy state. The formation of glass by continuous cooling (viscous slowdown) is then examined, the strong/fragile liquids classif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Angell CA

更新日期：1995-07-18 00:00:00

abstract：:In vivo electroporation is a powerful technique for the introduction of genes into organisms. Temporal and spatial regulation of expression of introduced genes, or of RNAi, would further enhance the utility of this method. Here we demonstrate conditional regulation of gene expression from electroporated plasmids in th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsuda T,Cepko CL

更新日期：2007-01-16 00:00:00

abstract：:Selective inhibition of the intrinsic coagulation pathway is a promising strategy for developing safer anticoagulants that do not cause serious bleeding. Intrinsic tenase, the final and rate-limiting enzyme complex in the intrinsic coagulation pathway, is an attractive but less explored target for anticoagulants due t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhao L,Wu M,Xiao C,Yang L,Zhou L,Gao N,Li Z,Chen J,Chen J,Liu J,Qin H,Zhao J

更新日期：2015-07-07 00:00:00

abstract：:There are some ∼1,100 known antimicrobial peptides (AMPs), which permeabilize microbial membranes but have diverse sequences. Here, we develop a support vector machine (SVM)-based classifier to investigate ⍺-helical AMPs and the interrelated nature of their functional commonality and sequence homology. SVM is used to ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lee EY,Fulan BM,Wong GC,Ferguson AL

更新日期：2016-11-29 00:00:00

abstract：:We advance a theory of resilience as it applies to the challenges of international development. The conceptualization we advance for development resilience focuses on the stochastic dynamics of individual and collective human well-being, especially on the avoidance of and escape from chronic poverty over time in the f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barrett CB,Constas MA

更新日期：2014-10-07 00:00:00

abstract：:In all three domains of life, DNA replication begins at specialized loci termed replication origins. In bacteria, replication initiates from a single, clearly defined site. In contrast, eukaryotic organisms exploit a multitude of replication origins, dividing their genomes into an array of short contiguous units. Rece...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson NP,Bell SD

更新日期：2007-04-03 00:00:00

abstract：:Transgenic crops producing Bacillus thuringiensis (Bt) toxins kill some key insect pests and thus can reduce reliance on insecticides. Widespread planting of such Bt crops increased concerns that their usefulness would be cut short by rapid evolution of resistance to Bt toxins by pests. Pink bollworm (Pectinophora gos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tabashnik BE,Dennehy TJ,Carrière Y

更新日期：2005-10-25 00:00:00

abstract：:The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. By using somatic cell hybrids and in situ chromosomal hybridization, we localized this gene to human chromosome 5 at bands q23-31, a chromos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Le Beau MM,Epstein ND,O'Brien SJ,Nienhuis AW,Yang YC,Clark SC,Rowley JD

更新日期：1987-08-01 00:00:00

abstract：:Short oxygen-halogen interactions have been known in organic chemistry since the 1950s and recently have been exploited in the design of supramolecular assemblies. The present survey of protein and nucleic acid structures reveals similar halogen bonds as potentially stabilizing inter- and intramolecular interactions t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Auffinger P,Hays FA,Westhof E,Ho PS

更新日期：2004-11-30 00:00:00