The Order of the Antipode of Finite-dimensional Hopf Algebra.

abstract：:The basidiomycete Ustilago maydis is the causative agent of the disease corn smut. To be pathogenic, strains of U. maydis must be heterozygous for a locus called "b," which appears to control both pathogenicity and sexual development. Two alleles of the b locus of U. maydis were isolated by complementation and hybridi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kronstad JW,Leong SA

更新日期：1989-02-01 00:00:00

abstract：:Mutations at position 187 in secreted gelsolin enable aberrant proteolysis at the 172-173 and 243-244 amide bonds, affording the 71-residue amyloidogenic peptide deposited in Familial Amyloidosis of Finnish Type (FAF). Thermodynamic comparisons of two different domain 2 constructs were carried out to study possible ef...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ratnaswamy G,Huff ME,Su AI,Rion S,Kelly JW

更新日期：2001-02-27 00:00:00

abstract：:Although microtubules (MTs) frequently form highly dynamic networks, subsets of MTs become stabilized in response to environmental cues and function as specialized tracks for vesicle and macromolecular trafficking. MT stabilization is controlled by specialized plus-end tracking proteins (+TIPs) whose accumulation at t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Naghavi MH,Gundersen GG,Walsh D

更新日期：2013-11-05 00:00:00

abstract：:Linear porphyrin arrays self-assembled by either hydrogen bonding or metal ion coordination self-organize into lipid bilayer membranes. The length of the transmembrane assemblies is determined both by the thermodynamics of the intermolecular interactions in the supermolecule and by the dimension and physical chemical ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Drain CM

更新日期：2002-04-16 00:00:00

abstract：:Nuclear import of classical nuclear localization sequence-bearing proteins is mediated by karyopherin alpha/beta1 heterodimers. A second nuclear import pathway, mediated by karyopherin beta2 (transportin), recently was described for mRNA-binding proteins. Here we report the cloning and characterization of human karyop...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yaseen NR,Blobel G

更新日期：1997-04-29 00:00:00

abstract：:Electron cryomicroscopy (cryo-EM) has been used to determine the atomic coordinates (models) from density maps of biological assemblies. These models can be assessed by their overall fit to the experimental data and stereochemical information. However, these models do not annotate the actual density values of the atom...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hryc CF,Chen DH,Afonine PV,Jakana J,Wang Z,Haase-Pettingell C,Jiang W,Adams PD,King JA,Schmid MF,Chiu W

更新日期：2017-03-21 00:00:00

abstract：:A study was made of the effects of di- and trivalent cations on homomeric rho 1-type gamma-aminobutyrate (GABA rho 1) receptors expressed in Xenopus oocytes after injection of mRNA coding for the GABA rho 1 subunit. GABA elicited large currents with a Kd approximately 1 microM. The properties of these GABA rho 1 recep...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Calvo DJ,Vazquez AE,Miledi R

更新日期：1994-12-20 00:00:00

abstract：:Oligosaccharyltransferase transfers an oligosaccharide chain to the asparagine residues in proteins. The archaeal and eubacterial oligosaccharyltransferases are single subunit membrane enzymes, referred to as "AglB" (archaeal glycosylation B) and "PglB" (protein glycosylation B), respectively. Only one crystal structu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matsumoto S,Shimada A,Nyirenda J,Igura M,Kawano Y,Kohda D

更新日期：2013-10-29 00:00:00

abstract：:The minimal size of the recombination site required for efficient FLP recombinase-catalyzed recombination in vitro is no more than 28 base pairs, which includes parts of two 13-base-pair inverted repeats and all of an 8-base-pair spacer. The FLP recombinase cleaves the DNA at the boundaries of the spacer, becomes cova...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Senecoff JF,Bruckner RC,Cox MM

更新日期：1985-11-01 00:00:00

abstract：:A fraction of mouse RNA containing messenger RNA coding for mouse interferon was translated with high efficiency in a wheat germ system into a fully active product. This product fulfills the criteria for mouse interferon, namely: (1) it was active against vesicular stomatitis virus and herpes simplex virus in mouse ce...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thang MN,Thang DC,De Maeyer E,Montagnier L

更新日期：1975-10-01 00:00:00

abstract：:Infection of HeLa cells with poliovirus results in an inhibition of host-cell protein synthesis. Cytoplasmic extracts from infected cells contian an activity that inhibits initiation of protein synthesis in vitro by rabbit reticulocyte lysates. This inhibitory activity has been purified and characterized, and is shown...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ehrenfeld E,Hunt T

更新日期：1971-05-01 00:00:00

abstract：:The oncoproteins MDM2 and MDMX negatively regulate the activity and stability of the tumor suppressor protein p53--a cellular process initiated by MDM2 and/or MDMX binding to the N-terminal transactivation domain of p53. MDM2 and MDMX in many tumors confer p53 inactivation and tumor survival, and are important molecul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pazgier M,Liu M,Zou G,Yuan W,Li C,Li C,Li J,Monbo J,Zella D,Tarasov SG,Lu W

更新日期：2009-03-24 00:00:00

abstract：:To investigate whether caveolin-1 (cav-1) may modulate inducible nitric oxide synthase (iNOS) function in intact cells, the human intestinal carcinoma cell lines HT29 and DLD1 that have low endogenous cav-1 levels were transfected with cav-1 cDNA. In nontransfected cells, iNOS mRNA and protein levels were increased by...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felley-Bosco E,Bender FC,Courjault-Gautier F,Bron C,Quest AF

更新日期：2000-12-19 00:00:00

abstract：:Given their relatively small area, mangroves and their organic sediments are of disproportionate importance to global carbon sequestration and carbon storage. Peat deposition and preservation allows some mangroves to accrete vertically and keep pace with sea-level rise by growing on their own root remains. In this stu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ezcurra P,Ezcurra E,Garcillán PP,Costa MT,Aburto-Oropeza O

更新日期：2016-04-19 00:00:00

abstract：:Using an FK506 affinity column to identify mammalian immunosuppressant-binding proteins, we identified an immunophilin with an apparent M(r) approximately 55,000, which we have named FKBP52. We used chemically determined peptide sequence and a computerized algorithm to search GenPept, the translated GenBank data base,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peattie DA,Harding MW,Fleming MA,DeCenzo MT,Lippke JA,Livingston DJ,Benasutti M

更新日期：1992-11-15 00:00:00

abstract：:Adult rats were trained to detect the occurrence of a two-element sound sequence in a background of nine other nontarget sound pairs. Training resulted in a modest, enduring, static expansion of the cortical areas of representation of both target stimulus sounds. More importantly, once the initial stimulus A in the ta...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhou X,de Villers-Sidani E,Panizzutti R,Merzenich MM

更新日期：2010-08-17 00:00:00

abstract：:The nanoscale distributions of electron density and electric fields in GaAs semiconductor devices are displayed with NMR experiments. The spectra are sensitive to the changes to the nuclear-spin Hamiltonian that are induced by perturbations delivered in synchrony with a line-narrowing pulse sequence. This POWER (pertu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kempf JG,Miller MA,Weitekamp DP

更新日期：2008-12-23 00:00:00

abstract：:A method is described for monitoring the enzyme-mediated unwinding of duplex DNA spectrophotometrically. The assay employs a partially duplex oligonucleotide substrate modified at the complementary end with coumarin and fluorescein moieties. When in close proximity the fluorescein quenches the fluorescence of coumarin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Houston P,Kodadek T

更新日期：1994-06-07 00:00:00

abstract：:We present here a brief review of direct force measurements between hydrophobic surfaces in aqueous solutions. For almost 70 years, researchers have attempted to understand the hydrophobic effect (the low solubility of hydrophobic solutes in water) and the hydrophobic interaction or force (the unusually strong attract...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meyer EE,Rosenberg KJ,Israelachvili J

更新日期：2006-10-24 00:00:00

abstract：:A mitochondrial gene from Saccharomyces cerevisiae encoding a hydrophobic membrane protein, subunit 8 of the F0/F1-type mitochondrial ATPase complex, has been functionally replaced by an artificial nuclear gene specifying an imported version of this protein. The experiments reported here utilized a multicopy expressio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagley P,Farrell LB,Gearing DP,Nero D,Meltzer S,Devenish RJ

更新日期：1988-04-01 00:00:00

abstract：:Human diploid fibroblasts underwent lysis when resuspended in isotonic sucrose. After preparation of a granular fraction by differential centrifugation, lysosomes were almost completely purified by free-flow electrophoresis. Electron micrographs of the final fraction of fibroblast lysosomes (about 25-fold purified) sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Harms E,Kern H,Schneider JA

更新日期：1980-10-01 00:00:00

abstract：:The pattern of antibody specificities in sera from patients with myasthenia gravis (MG) was determined by the ability of monoclonal antibodies against defined determinants on the acetylcholine receptor molecule to inhibit binding of the serum antibodies to receptor from human muscle. We found that MG patients produce ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tzartos SJ,Seybold ME,Lindstrom JM

更新日期：1982-01-01 00:00:00

abstract：:Intact squid axons were injected with aequorin and bathed in 3 mM Ca seawater (a concentration close to that of squid blood). Sodium and potassium currents were pharmacologically blocked and repetitive voltage-clamp pulses of a duration of 1.5 ms were applied (to simulate the duration of an action potential) at amplit...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mullins LJ,Requena J,Whittembury J

更新日期：1985-03-01 00:00:00

abstract：:Five peptides corresponding to amino acid sequences predicted from all three reading frames of the nucleotide sequence of the third internal repeat array (IR3) of the Epstein-Barr virus (EBV) genome were synthesized chemically. All five peptides elicited antipeptide antibodies in rabbits. The antiserum raised against ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dillner J,Sternås L,Kallin B,Alexander H,Ehlin-Henriksson B,Jörnvall H,Klein G,Lerner R

更新日期：1984-08-01 00:00:00

abstract：:Acetyl-CoA synthase from Clostridium thermoaceticum (ACS(Ct)) is an alpha(2)beta(2) tetramer containing two novel Ni-X-Fe(4)S(4) active sites (the A and C clusters) and a standard Fe(4)S(4) cluster (the B cluster). The acsA and acsB genes encoding the enzyme were cloned into Escherichia coli strain JM109 and overexpre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Loke HK,Bennett GN,Lindahl PA

更新日期：2000-11-07 00:00:00

abstract：:Prostate cancer is a leading cause of cancer death in men over 50 years of age, and there is a characteristic marked decrease in Zn content in the malignant prostate cells. The cause and consequences of this loss have thus far been unknown. We found that in middle-aged rats a Zn-deficient diet reduces prostatic Zn lev...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fong LY,Jing R,Smalley KJ,Wang ZX,Taccioli C,Fan S,Chen H,Alder H,Huebner K,Farber JL,Fiehn O,Croce CM

更新日期：2018-11-20 00:00:00

abstract：:To investigate protein folding dynamics in terms of compactness, we developed a continuous-flow mixing device to make small-angle x-ray scattering measurements with the time resolution of 160 micros and characterized the radius of gyration (R(g)) of two folding intermediates of cytochrome c (cyt c). The early intermed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama S,Takahashi S,Kimura T,Ishimori K,Morishima I,Nishikawa Y,Fujisawa T

更新日期：2002-02-05 00:00:00

abstract：:RNA interference (RNAi) is a widely used method for analysis of gene function in tissue culture cells. However, to date there has been no reliable method for testing the specificity of any particular RNAi experiment. The ideal experiment is to rescue the phenotype by expression of the target gene in a form refractory ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kittler R,Pelletier L,Ma C,Poser I,Fischer S,Hyman AA,Buchholz F

更新日期：2005-02-15 00:00:00

abstract：:Herpes simplex virus type 1 (HSV-1) thymidine kinase is currently used as a suicide agent in the gene therapy of cancer. This therapy is based on the preferential phosphorylation of nucleoside analogs by tumor cells expressing HSV-1 thymidine kinase. However, the use of HSV-1 thymidine kinase is limited in part by the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Black ME,Newcomb TG,Wilson HM,Loeb LA

更新日期：1996-04-16 00:00:00

abstract：:X-linked hypophosphatemia is the most prevalent inherited form of rickets. In this disorder, rickets results from hyperphosphaturia and inappropriately normal levels of 1,25(OH)2-vitamin D. Current therapy with oral phosphate and vitamin D improves the rickets, but has significant morbidity and does not significantly ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Baum M,Loleh S,Saini N,Seikaly M,Dwarakanath V,Quigley R

更新日期：2003-09-16 00:00:00