Active acetyl-CoA synthase from Clostridium thermoaceticum obtained by cloning and heterologous expression of acsAB in Escherichia coli.

abstract：:The Ca(2+)-binding protein calbindin D28k (CB) is vital for the normal function of the central nervous system but its specific functional role is largely unclear. CB is typically described as a mobile Ca(2+)buffer that shapes the spatiotemporal extent of cellular Ca(2+)signals. Recent biochemical data, however, indica...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schmidt H,Schwaller B,Eilers J

更新日期：2005-04-19 00:00:00

abstract：:It is proved that the well known Nagata automorphism of the polynomial ring in three variables over a field of characteristic zero is wild, that is, it can not be decomposed into a product of elementary automorphisms. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shestakov IP,Umirbaev UU

更新日期：2003-10-28 00:00:00

abstract：:Many membrane channels and receptors exhibit adaptive, or desensitized, response to a strong sustained input stimulus. A key mechanism that underlies this response is the slow, activity-dependent removal of responding molecules to a pool which is unavailable to respond immediately to the input. This mechanism is imple...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Friedlander T,Brenner N

更新日期：2009-12-29 00:00:00

abstract：:Accurate protein folding is essential for proper cellular and organismal function. In the cell, protein folding is carefully regulated; changes in folding homeostasis (proteostasis) can disrupt many cellular processes and have been implicated in various neurodegenerative diseases and other pathologies. For many protei...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Samelson AJ,Jensen MK,Soto RA,Cate JH,Marqusee S

更新日期：2016-11-22 00:00:00

abstract：:Individual diastereomeric phosphoramidites and mixtures of diastereomeric phosphoramidites were evaluated in the iridium-catalyzed amination of allylic carbonates. The original process was conducted with a phosphoramidite ligand containing a resolved 2,2-dihydroxy-1,1-binaphthyl (BINOL) group and a diastereomerically ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Leitner A,Shu C,Hartwig JF

更新日期：2004-04-20 00:00:00

abstract：:Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) genes encode transcription factors belonging to the orphan subfamily of the steroid/thyroid hormone receptor superfamily. Two COUP-TF counterparts have been cloned from mouse. In an attempt to study the function of these genes in the developing central...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Qiu Y,Cooney AJ,Kuratani S,DeMayo FJ,Tsai SY,Tsai MJ

更新日期：1994-05-10 00:00:00

abstract：:Homologs of the chromatin-bound yeast silent information regulator 2 (SIR2) protein are found in organisms from all biological kingdoms. SIR2 itself was originally discovered to influence mating-type control in haploid cells by locus-specific transcriptional silencing. Since then, SIR2 and its homologs have been sugge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Landry J,Sutton A,Tafrov ST,Heller RC,Stebbins J,Pillus L,Sternglanz R

更新日期：2000-05-23 00:00:00

abstract：:Although ghrelin has been demonstrated to stimulate energy intake and storage through a central mechanism, its effect on hepatic lipid metabolism remains largely uncharacterized. Ghrelin receptor antagonism or gene deletion significantly decreased obesity-associated hepatic steatosis by suppression of de novo lipogene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Z,Xu G,Qin Y,Zhang C,Tang H,Yin Y,Xiang X,Li Y,Zhao J,Mulholland M,Zhang W

更新日期：2014-09-09 00:00:00

abstract：:Neural crest cell derivatives have been suggested to be involved in thymus development. We established nonlymphoid thymic stromal cell cultures capable of supporting T-cell differentiation. In these nonlymphoid cell cultures, we identified cells with phenotypic and biochemical markers specific for neuronal cells. Neur...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Screpanti I,Meco D,Scarpa S,Morrone S,Frati L,Gulino A,Modesti A

更新日期：1992-04-15 00:00:00

abstract：:A deficit in early clearance of Pseudomonas aeruginosa (P. aeruginosa) is crucial in nosocomial pneumonia and in chronic lung infections. Few studies have addressed the role of Toll-like receptors (TLRs), which are early pathogen associated molecular pattern receptors, in pathogen uptake and clearance by alveolar macr...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Descamps D,Le Gars M,Balloy V,Barbier D,Maschalidi S,Tohme M,Chignard M,Ramphal R,Manoury B,Sallenave JM

更新日期：2012-01-31 00:00:00

abstract：:Recent evidence indicates a specific role for vascular endothelial growth factor a (Vegfa) during artery development in both zebrafish and mouse embryos, whereas less is known about signals that govern vein formation. In zebrafish, loss of vegfa blocks segmental artery formation and reduces artery-specific gene expres...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Covassin LD,Villefranc JA,Kacergis MC,Weinstein BM,Lawson ND

更新日期：2006-04-25 00:00:00

abstract：:Ice-core records show that climate changes in the past have been large, rapid, and synchronous over broad areas extending into low latitudes, with less variability over historical times. These ice-core records come from high mountain glaciers and the polar regions, including small ice caps and the large ice sheets of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alley RB

更新日期：2000-02-15 00:00:00

abstract：:Little is known about the molecular mechanisms by which the embryo proper and suspensor of plant embryos activate specific gene sets shortly after fertilization. We analyzed the upstream region of the scarlet runner bean (Phaseolus coccineus) G564 gene to understand how genes are activated specifically within the susp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kawashima T,Wang X,Henry KF,Bi Y,Weterings K,Goldberg RB

更新日期：2009-03-03 00:00:00

abstract：:The sided effects of H+ on Ca2(+)-stimulated K+ transport (the Gardos channel) were studied in human red blood cells. Cells were loaded with Ca2+ during energy depletion with the internal pH adjusted to desired levels prior to treatment with the anion-exchange inhibitor 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heinz A,Hoffman JF

更新日期：1990-03-01 00:00:00

abstract：:Restricted fetal growth is associated with postnatal mortality and morbidity and may be directly related to alterations in the capacity of the placenta to supply nutrients. We proposed previously that imprinted genes can regulate nutrient supply by the placenta. Here, we tested the hypothesis that the insulin-like gro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sibley CP,Coan PM,Ferguson-Smith AC,Dean W,Hughes J,Smith P,Reik W,Burton GJ,Fowden AL,Constância M

更新日期：2004-05-25 00:00:00

abstract：:Chronic hypoxia causes pulmonary hypertension with smooth muscle cell proliferation and matrix deposition in the wall of the pulmonary arterioles. We demonstrate here that hypoxia also induces a pronounced inflammation in the lung before the structural changes of the vessel wall. The proinflammatory action of hypoxia ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Minamino T,Christou H,Hsieh CM,Liu Y,Dhawan V,Abraham NG,Perrella MA,Mitsialis SA,Kourembanas S

更新日期：2001-07-17 00:00:00

abstract：:The endothelins (ETs) form a group of three vasoactive peptides (ET-1, ET-2, and ET-3) for which two types of cellular receptors have been identified, types A and B ET receptors (ETA and ETB receptors, respectively). To address possible targets for ETs within the liver, we isolated the four principal liver cell popula...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Housset C,Rockey DC,Bissell DM

更新日期：1993-10-15 00:00:00

abstract：:A survey was performed to estimate the frequency of enterohemorrhagic Escherichia coli O157:H7 or O157:nonmotile (EHEC O157) in feces and on hides within groups of fed cattle from single sources (lots) presented for slaughter at meat processing plants in the Midwestern United States, as well as frequency of carcass co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elder RO,Keen JE,Siragusa GR,Barkocy-Gallagher GA,Koohmaraie M,Laegreid WW

更新日期：2000-03-28 00:00:00

abstract：:Immunoaffinity-purified interleukin 2 (IL2) stimulated proliferation of large but not small B cells. Stimulation was observed even when B cells were cultured at very low cell densities (3 X 10(4) per microwell containing 0.2 ml of medium). Addition of small numbers of purified splenic T cells did not enhance the IL2-i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mond JJ,Thompson C,Finkelman FD,Farrar J,Schaefer M,Robb RJ

更新日期：1985-03-01 00:00:00

abstract：:Probing a wide range of cellular phenotypes in neurodevelopmental disorders using patient-derived neural progenitor cells (NPCs) can be facilitated by 3D assays, as 2D systems cannot entirely recapitulate the arrangement of cells in the brain. Here, we developed a previously unidentified 3D migration and differentiati...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zhang ZN,Freitas BC,Qian H,Lux J,Acab A,Trujillo CA,Herai RH,Nguyen Huu VA,Wen JH,Joshi-Barr S,Karpiak JV,Engler AJ,Fu XD,Muotri AR,Almutairi A

更新日期：2016-03-22 00:00:00

abstract：:For a series of different proteins, including a structural protein, enzyme, inhibitor, protein marker, and a charge-transfer system, we have quantified the higher affinity of Na+ over K+ to the protein surface by means of molecular dynamics simulations and conductivity measurements. Both approaches show that sodium bi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vrbka L,Vondrásek J,Jagoda-Cwiklik B,Vácha R,Jungwirth P

更新日期：2006-10-17 00:00:00

abstract：:The temperature dependence of migration rate and of the thermotactic sensitivity of pseudoplasmodia of Dictyostelium discoideum has been measured. Migration rate increases with temperature to 20 degrees, is temperature insensitive from 20 degrees to 27.5 degrees, and decreases with temperature to 29 degrees, above whi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Poff KL,Skokut M

更新日期：1977-05-01 00:00:00

abstract：:Exposure of 3T3/A31 cells to serum-free medium, one type of apoptotic stimulus, causes a rapid increase in the sphingosine (Sph) level, which initiates a series of processes: (i) activation of caspase 3 through an enhanced "cascade" of caspases, (ii) release of the C-terminal-half kinase domain of PKCdelta (PKCdelta K...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suzuki E,Handa K,Toledo MS,Hakomori S

更新日期：2004-10-12 00:00:00

abstract：:Understanding the mechanisms of nanoparticle interaction with cell membranes is essential for designing materials for applications such as bioimaging and drug delivery, as well as for assessing engineered nanomaterial safety. Much attention has focused on nanoparticles that bind strongly to biological membranes or ind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Foreman-Ortiz IU,Liang D,Laudadio ED,Calderin JD,Wu M,Keshri P,Zhang X,Schwartz MP,Hamers RJ,Rotello VM,Murphy CJ,Cui Q,Pedersen JA

更新日期：2020-11-10 00:00:00

abstract：:Growth and differentiation factor 11 (GDF11) and myostatin (MSTN) are closely related transforming growth factor β (TGF-β) family members, but their biological functions are quite distinct. While MSTN has been widely shown to inhibit muscle growth, GDF11 regulates skeletal patterning and organ development during embry...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Suh J,Kim NK,Lee SH,Eom JH,Lee Y,Park JC,Woo KM,Baek JH,Kim JE,Ryoo HM,Lee SJ,Lee YS

更新日期：2020-03-03 00:00:00

abstract：:Phosphorylation of inositol phospholipids plays a key role in cellular regulation via the generation of intracellular second messengers. In addition, it represents a mechanism to regulate interactions of the lipid bilayer with proteins and protein scaffolds involved in vesicle budding, cytoskeletal organization, and s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Guo J,Wenk MR,Pellegrini L,Onofri F,Benfenati F,De Camilli P

更新日期：2003-04-01 00:00:00

abstract：:The human intestine is densely populated by a microbial consortium whose metabolic activities are influenced by, among others, bifidobacteria. However, the genetic basis of adaptation of bifidobacteria to the human gut is poorly understood. Analysis of the 2,214,650-bp genome of Bifidobacterium bifidum PRL2010, a stra...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Turroni F,Bottacini F,Foroni E,Mulder I,Kim JH,Zomer A,Sánchez B,Bidossi A,Ferrarini A,Giubellini V,Delledonne M,Henrissat B,Coutinho P,Oggioni M,Fitzgerald GF,Mills D,Margolles A,Kelly D,van Sinderen D,Ventura M

更新日期：2010-11-09 00:00:00

abstract：:gamma-Aminobutyric acid type A (GABA-A) receptors are a major mediator of inhibitory neurotransmission in the mammalian central nervous system, and the site of action of a number of clinically important drugs. These receptors exist as a family of subtypes with distinct temporal and spatial patterns of expression and d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bonnert TP,McKernan RM,Farrar S,le Bourdellès B,Heavens RP,Smith DW,Hewson L,Rigby MR,Sirinathsinghji DJ,Brown N,Wafford KA,Whiting PJ

更新日期：1999-08-17 00:00:00

abstract：:The methyl-CpG dinucleotide containing a symmetrical 5-methylcytosine (mC) is involved in gene regulation and genome stability. We report here that methylation-mediated transcriptional repressor methylated DNA-binding domain 1 (MBD1) interacts with methylpurine-DNA glycosylase (MPG), which excises damaged bases from s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Watanabe S,Ichimura T,Fujita N,Tsuruzoe S,Ohki I,Shirakawa M,Kawasuji M,Nakao M

更新日期：2003-10-28 00:00:00

abstract：:Long-term memory relies on modulation of synaptic connections in response to experience. This plasticity involves trafficking of AMPA receptors (AMPAR) and alteration of spine morphology. Arc, a gene induced by synaptic activity, mediates the endocytosis of AMPA receptors and is required for both long-term and homeost...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Peebles CL,Yoo J,Thwin MT,Palop JJ,Noebels JL,Finkbeiner S

更新日期：2010-10-19 00:00:00