Abstract:
:Queuosine (Q) is a complex tRNA modification widespread in eukaryotes and bacteria that contributes to the efficiency and accuracy of protein synthesis. Eukaryotes are not capable of Q synthesis and rely on salvage of the queuine base (q) as a Q precursor. While many bacteria are capable of Q de novo synthesis, salvage of the prokaryotic Q precursors preQ0 and preQ1 also occurs. With the exception of Escherichia coli YhhQ, shown to transport preQ0 and preQ1, the enzymes and transporters involved in Q salvage and recycling have not been well described. We discovered and characterized 2 Q salvage pathways present in many pathogenic and commensal bacteria. The first, found in the intracellular pathogen Chlamydia trachomatis, uses YhhQ and tRNA guanine transglycosylase (TGT) homologs that have changed substrate specificities to directly salvage q, mimicking the eukaryotic pathway. The second, found in bacteria from the gut flora such as Clostridioides difficile, salvages preQ1 from q through an unprecedented reaction catalyzed by a newly defined subgroup of the radical-SAM enzyme family. The source of q can be external through transport by members of the energy-coupling factor (ECF) family or internal through hydrolysis of Q by a dedicated nucleosidase. This work reinforces the concept that hosts and members of their associated microbiota compete for the salvage of Q precursors micronutrients.
journal_name
Proc Natl Acad Sci U S Aauthors
Yuan Y,Zallot R,Grove TL,Payan DJ,Martin-Verstraete I,Šepić S,Balamkundu S,Neelakandan R,Gadi VK,Liu CF,Swairjo MA,Dedon PC,Almo SC,Gerlt JA,de Crécy-Lagard Vdoi
10.1073/pnas.1909604116subject
Has Abstractpub_date
2019-09-17 00:00:00pages
19126-19135issue
38eissn
0027-8424issn
1091-6490pii
1909604116journal_volume
116pub_type
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