Functional expression of a human Na+/H+ antiporter gene transfected into antiporter-deficient mouse L cells.

Abstract:

:To clone the gene for the human Na+/H+ antiporter, we first constructed a stable mouse LTK- cell line (LAP1) lacking Na+/H+ antiport activity. Second, we devised a selective technique based on acid killing that specifically sorts out cells expressing low levels of Na+/H+ antiport activity from a population of antiporter-deficient cells (AP-). LAP1 cells (TK- and AP-) were cotransformed with human genomic DNA and the thymidine kinase (TK) gene. TK+ transformants, first selected, were submitted to acid loading. The rare transformants that survived (frequency, 2-8 X 10(-7) expressed Na+/H+ antiport activity (AP+). We found that: transformation with mouse LAP1 DNA did not give rise to AP+ transformants; transformation of LAP1 cells with DNA from an altered Na+/H+ antiporter hamster variant led to AP+ transformants expressing the altered Na+/H+ antiporter of the DNA donor; human repeated sequences were present in all primary, secondary, and tertiary mouse AP+ transformants; six identical EcoRI human DNA fragments (55 kilobase pairs of the human genome) cosegregated with the Na+/H+ antiport activity in secondary and tertiary transformants. These results strongly suggest that we have stably expressed the structural gene for the human Na+/H+ antiporter in mouse cells.

authors

Franchi A,Perucca-Lostanlen D,Pouyssegur J

doi

10.1073/pnas.83.24.9388

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

9388-92

issue

24

eissn

0027-8424

issn

1091-6490

journal_volume

83

pub_type

杂志文章
  • Pituitary adenylate cyclase-activating polypeptide protects rat cerebellar granule neurons against ethanol-induced apoptotic cell death.

    abstract::Alcohol exposure during development can cause brain malformations and neurobehavioral abnormalities. In view of the teratogenicity of ethanol, identification of molecules that could counteract the neurotoxic effects of alcohol deserves high priority. Here, we report that pituitary adenylate cyclase-activating polypept...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.082112699

    authors: Vaudry D,Rousselle C,Basille M,Falluel-Morel A,Pamantung TF,Fontaine M,Fournier A,Vaudry H,Gonzalez BJ

    更新日期:2002-04-30 00:00:00

  • Chromosomal insertion of foreign (adenovirus type 12, plasmid, or bacteriophage lambda) DNA is associated with enhanced methylation of cellular DNA segments.

    abstract::Insertion of foreign DNA into an established mammalian genome can extensively alter the patterns of cellular DNA methylation. Adenovirus type 12 (Ad12)-transformed hamster cells, Ad12-induced hamster tumor cells, or hamster cells carrying integrated DNA of bacteriophage lambda were used as model systems. DNA methylati...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.92.12.5515

    authors: Heller H,Kämmer C,Wilgenbus P,Doerfler W

    更新日期:1995-06-06 00:00:00

  • Cloning and sequencing of the pertussis toxin genes: operon structure and gene duplication.

    abstract::Pertussis toxin, a protein composed of five different subunits (S1, S2, S3, S4, and S5), is the major virulence factor of Bordetella pertussis. We have cloned and sequenced a DNA fragment of 4.7 kilobases that contains the genes coding for the five subunits. The genes are clustered within 3.2 kilobases in the followin...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.83.13.4631

    authors: Nicosia A,Perugini M,Franzini C,Casagli MC,Borri MG,Antoni G,Almoni M,Neri P,Ratti G,Rappuoli R

    更新日期:1986-07-01 00:00:00

  • Accurately quantifying low-abundant targets amid similar sequences by revealing hidden correlations in oligonucleotide microarray data.

    abstract::Microarrays have enabled the determination of how thousands of genes are expressed to coordinate function within single organisms. Yet applications to natural or engineered communities where different organisms interact to produce complex properties are hampered by theoretical and technological limitations. Here we de...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0601476103

    authors: Marcelino LA,Backman V,Donaldson A,Steadman C,Thompson JR,Preheim SP,Lien C,Lim E,Veneziano D,Polz MF

    更新日期:2006-09-12 00:00:00

  • Insect immune response to bacterial infection is mediated by eicosanoids.

    abstract::Inhibition of eicosanoid formation in larvae of the tobacco hornworm Manduca sexta, using specific inhibitors of phospholipase A2, cyclooxygenase, and lipoxygenase, severely weakened the ability of larvae to clear the bacterium Serratia marscescens from their hemolymph. The reduced capability to remove bacteria is ass...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.88.3.1064

    authors: Stanley-Samuelson DW,Jensen E,Nickerson KW,Tiebel K,Ogg CL,Howard RW

    更新日期:1991-02-01 00:00:00

  • WNK4 regulates activity of the epithelial Na+ channel in vitro and in vivo.

    abstract::Homeostasis of intravascular volume, Na(+), Cl(-), and K(+) is interdependent and determined by the coordinated activities of structurally diverse mediators in the distal nephron and the distal colon. The behavior of these flux pathways is regulated by the renin-angiotensin-aldosterone system; however, the mechanisms ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0611727104

    authors: Ring AM,Cheng SX,Leng Q,Kahle KT,Rinehart J,Lalioti MD,Volkman HM,Wilson FH,Hebert SC,Lifton RP

    更新日期:2007-03-06 00:00:00

  • Regulation of the GABA cell phenotype in hippocampus of schizophrenics and bipolars.

    abstract::GABAergic dysfunction is present in the hippocampus in schizophrenia (SZ) and bipolar disorder (BD). The trisynaptic pathway was "deconstructed" into various layers of sectors CA3/2 and CA1 and gene expression profiling performed. Network association analysis was used to uncover genes that may be related to regulation...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0703806104

    authors: Benes FM,Lim B,Matzilevich D,Walsh JP,Subburaju S,Minns M

    更新日期:2007-06-12 00:00:00

  • Encoding biological recognition in a bicomponent cell-membrane mimic.

    abstract::Self-assembling dendrimers have facilitated the discovery of periodic and quasiperiodic arrays of supramolecular architectures and the diverse functions derived from them. Examples are liquid quasicrystals and their approximants plus helical columns and spheres, including some that disregard chirality. The same period...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1821924116

    authors: Rodriguez-Emmenegger C,Xiao Q,Kostina NY,Sherman SE,Rahimi K,Partridge BE,Li S,Sahoo D,Reveron Perez AM,Buzzacchera I,Han H,Kerzner M,Malhotra I,Möller M,Wilson CJ,Good MC,Goulian M,Baumgart T,Klein ML,Percec V

    更新日期:2019-03-19 00:00:00

  • Rat cardiac ventricle has two Na+,K+-ATPases with different affinities for ouabain: developmental changes in immunologically different catalytic subunits.

    abstract::The mechanism of the inotropic effect of cardiac glycosides on the heart has long been controversial. Inotropic effects at low concentrations of cardiac glycosides indicate more than one class of receptor or more than one cellular mechanism. In the brain of the rat, high- and low-affinity cardiac glycoside receptors h...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.84.23.8404

    authors: Sweadner KJ,Farshi SK

    更新日期:1987-12-01 00:00:00

  • Monkeypox virus evades antiviral CD4+ and CD8+ T cell responses by suppressing cognate T cell activation.

    abstract::Monkeypox virus (MPV) is a virulent human pathogen that has gained increased attention because of its potential use as a bioterrorism agent and inadvertent introduction into North America in 2003. The US outbreak also provided an important opportunity to study MPV-specific T cell immunity. Although MPV-specific CD4(+)...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0800589105

    authors: Hammarlund E,Dasgupta A,Pinilla C,Norori P,Früh K,Slifka MK

    更新日期:2008-09-23 00:00:00

  • Comparison of the complete nucleotide sequences of the genomes of the neurovirulent poliovirus P3/Leon/37 and its attenuated Sabin vaccine derivative P3/Leon 12a1b.

    abstract::As part of a study into the molecular basis of attenuation and reversion to neurovirulence in the Sabin poliovirus vaccines, we have determined the complete nucleotide sequence of a cloned DNA copy of the genome of P3/Leon/37, the neurovirulent progenitor of the type 3 Sabin vaccine strain, P3/Leon 12a1b. Comparison o...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.81.5.1539

    authors: Stanway G,Hughes PJ,Mountford RC,Reeve P,Minor PD,Schild GC,Almond JW

    更新日期:1984-03-01 00:00:00

  • Activation of murine c-abl protooncogene: effect of a point mutation on oncogenic activation.

    abstract::Activation of the c-abl protooncogene occurs in Abelson murine leukemia virus, in Hardy-Zuckerman 2 feline sarcoma virus, and during the chromosomal translocations that generate BCR-ABL gene fusion products. To study the molecular mechanism involved in the c-abl activation, we have created a series of modifications in...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.87.17.6502

    authors: Shore SK,Bogart SL,Reddy EP

    更新日期:1990-09-01 00:00:00

  • Host cell recognition by the henipaviruses: crystal structures of the Nipah G attachment glycoprotein and its complex with ephrin-B3.

    abstract::Nipah virus (NiV) and Hendra virus are the type species of the highly pathogenic paramyxovirus genus Henipavirus, which can cause severe respiratory disease and fatal encephalitis infections in humans, with case fatality rates approaching 75%. NiV contains two envelope glycoproteins, the receptor-binding G glycoprotei...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0804797105

    authors: Xu K,Rajashankar KR,Chan YP,Himanen JP,Broder CC,Nikolov DB

    更新日期:2008-07-22 00:00:00

  • Arsenic-induced PML targeting onto nuclear bodies: implications for the treatment of acute promyelocytic leukemia.

    abstract::Acute promyelocytic leukemia (APL) is associated with the t(15;17) translocation, which generates a PML/RAR alpha fusion protein between PML, a growth suppressor localized on nuclear matrix-associated bodies, and RAR alpha, a nuclear receptor for retinoic acid (RA). PML/RAR alpha was proposed to block myeloid differen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.94.8.3978

    authors: Zhu J,Koken MH,Quignon F,Chelbi-Alix MK,Degos L,Wang ZY,Chen Z,de Thé H

    更新日期:1997-04-15 00:00:00

  • Selective activation of the M1 muscarinic acetylcholine receptor achieved by allosteric potentiation.

    abstract::The forebrain cholinergic system promotes higher brain function in part by signaling through the M(1) muscarinic acetylcholine receptor (mAChR). During Alzheimer's disease (AD), these cholinergic neurons degenerate, therefore selectively activating M(1) receptors could improve cognitive function in these patients whil...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.0900903106

    authors: Ma L,Seager MA,Wittmann M,Jacobson M,Bickel D,Burno M,Jones K,Graufelds VK,Xu G,Pearson M,McCampbell A,Gaspar R,Shughrue P,Danziger A,Regan C,Flick R,Pascarella D,Garson S,Doran S,Kreatsoulas C,Veng L,Lindsley C

    更新日期:2009-09-15 00:00:00

  • Tracking sustainable development with a national barometer for South Africa using a downscaled "safe and just space" framework.

    abstract::Nations in the 21st century face a complex mix of environmental and social challenges, as highlighted by the on-going Sustainable Development Goals process. The "planetary boundaries" concept [Rockström J, et al. (2009) Nature 461(7263):472-475], and its extension through the addition of social well-being indicators t...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1400985111

    authors: Cole MJ,Bailey RM,New MG

    更新日期:2014-10-21 00:00:00

  • A clonal analysis of determination in Antennapedia a homoeotic mutant of Drosophila melanogaster.

    abstract::The process by which cells select one of several specific programs of genetic information in development was examined in homoeotic mutant Antennapedia of Rappaport in Drosophila melanogaster. The results show that the alteration of determination from antenna to leg induced by this homoeotic mutation occurs in a popula...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.64.1.176

    authors: Postlethwait JH,Schneiderman HA

    更新日期:1969-09-01 00:00:00

  • In vivo antagonism of a T cell response by an endogenously expressed ligand.

    abstract::3.L2 T cell receptor transgenic T cells are activated by the 64-76 peptide of the mouse hemoglobin d beta chain [Hb(64-76)], and their response is antagonized by the position 72 alanine substitution of this peptide (A72). To test the effect of this altered peptide ligand (APL) on 3.L2 T cell function in vivo, a transg...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.95.24.14332

    authors: Basu D,Williams CB,Allen PM

    更新日期:1998-11-24 00:00:00

  • Interaction of spatially separated protein-DNA complexes for control of gene expression: operator conversions.

    abstract::Two operators, spatially separated from each other and from the promoters, repress the gal operon when bound to Gal repressor. Conversion of either gal operator to a lac operator results in derepression, although both Gal and Lac repressors are present, suggesting that mere occupation of operator sites is not sufficie...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.85.24.9683

    authors: Haber R,Adhya S

    更新日期:1988-12-01 00:00:00

  • Habitual control of goal selection in humans.

    abstract::Humans choose actions based on both habit and planning. Habitual control is computationally frugal but adapts slowly to novel circumstances, whereas planning is computationally expensive but can adapt swiftly. Current research emphasizes the competition between habits and plans for behavioral control, yet many complex...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1506367112

    authors: Cushman F,Morris A

    更新日期:2015-11-10 00:00:00

  • Cytoplasmic poly(A) polymerases mediate cellular responses to S phase arrest.

    abstract::The S-M checkpoint delays mitosis until DNA replication is complete; cells defective in this checkpoint lose viability when DNA replication is inhibited. This inviability can be suppressed in fission yeast by overexpression of Cid1 or the related protein Cid13. Fission yeast contain six cid1/cid13-like genes, whereas ...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.192467799

    authors: Read RL,Martinho RG,Wang SW,Carr AM,Norbury CJ

    更新日期:2002-09-17 00:00:00

  • Induction of apoptotic DNA damage and cell death by activation of the sphingomyelin pathway.

    abstract::The potential involvement of ceramide-related signaling processes in the induction of apoptosis by tumor necrosis factor alpha was assessed by multiple biochemical strategies in the human leukemic cell lines HL-60 and U937 and the murine fibrosarcoma cell lines L929/LM and WEHI 164/13. Exposure of these cells to tumor...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.91.1.73

    authors: Jarvis WD,Kolesnick RN,Fornari FA,Traylor RS,Gewirtz DA,Grant S

    更新日期:1994-01-04 00:00:00

  • Cell envelope and shape of Escherichia coli K12.

    abstract::Rod-shaped "ghosts" that are free of murein have been isolated from E. coli. The shape of these "ghosts" is maintained by a unit membrane soluble in sodium dodecyl sulfate. Ghosts consist of about 20-30% phospholipid (almost exclusively phosphatidylethanolamine) and 50-60% protein; a large fraction of the remaining ma...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.70.7.2033

    authors: Henning U,Rehn K,Hoehn B

    更新日期:1973-07-01 00:00:00

  • Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface protein-1 locus.

    abstract::The organization and allelic recombination of the merozoite surface protein-1 gene of Plasmodium vivax (PvMsp-1), the most widely prevalent human malaria parasite, were evaluated in complete nucleotide sequences of 40 isolates from various geographic areas. Alignment of 31 distinct alleles revealed the mosaic organiza...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.252348999

    authors: Putaporntip C,Jongwutiwes S,Sakihama N,Ferreira MU,Kho WG,Kaneko A,Kanbara H,Hattori T,Tanabe K

    更新日期:2002-12-10 00:00:00

  • Glucagon receptor activates extracellular signal-regulated protein kinase 1/2 via cAMP-dependent protein kinase.

    abstract::We prepared a stable cell line expressing the glucagon receptor to characterize the effect of G(s)-coupled receptor stimulation on extracellular signal-regulated protein kinase 1/2 (ERK1/2) activity. Glucagon treatment of the cell line caused a dose-dependent increase in cAMP concentration, activation of cAMP-dependen...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.131200398

    authors: Jiang Y,Cypess AM,Muse ED,Wu CR,Unson CG,Merrifield RB,Sakmar TP

    更新日期:2001-08-28 00:00:00

  • BDNF pro-peptide actions facilitate hippocampal LTD and are altered by the common BDNF polymorphism Val66Met.

    abstract::Most growth factors are initially synthesized as precursor proteins and subsequently processed into their mature form by proteolytic cleavage, resulting in simultaneous removal of a pro-peptide. However, compared with that of mature form, the biological role of the pro-peptide is poorly understood. Here, we investigat...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1422336112

    authors: Mizui T,Ishikawa Y,Kumanogoh H,Lume M,Matsumoto T,Hara T,Yamawaki S,Takahashi M,Shiosaka S,Itami C,Uegaki K,Saarma M,Kojima M

    更新日期:2015-06-09 00:00:00

  • Identification of tissue-specific cell death using methylation patterns of circulating DNA.

    abstract::Minimally invasive detection of cell death could prove an invaluable resource in many physiologic and pathologic situations. Cell-free circulating DNA (cfDNA) released from dying cells is emerging as a diagnostic tool for monitoring cancer dynamics and graft failure. However, existing methods rely on differences in DN...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1519286113

    authors: Lehmann-Werman R,Neiman D,Zemmour H,Moss J,Magenheim J,Vaknin-Dembinsky A,Rubertsson S,Nellgård B,Blennow K,Zetterberg H,Spalding K,Haller MJ,Wasserfall CH,Schatz DA,Greenbaum CJ,Dorrell C,Grompe M,Zick A,Hubert A,M

    更新日期:2016-03-29 00:00:00

  • Galacturonosyltransferase (GAUT)1 and GAUT7 are the core of a plant cell wall pectin biosynthetic homogalacturonan:galacturonosyltransferase complex.

    abstract::Plant cell wall pectic polysaccharides are arguably the most complex carbohydrates in nature. Progress in understanding pectin synthesis has been slow due to its complex structure and difficulties in purifying and expressing the low-abundance, Golgi membrane-bound pectin biosynthetic enzymes. Arabidopsis galacturonosy...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1112816108

    authors: Atmodjo MA,Sakuragi Y,Zhu X,Burrell AJ,Mohanty SS,Atwood JA 3rd,Orlando R,Scheller HV,Mohnen D

    更新日期:2011-12-13 00:00:00

  • NAD tagSeq reveals that NAD+-capped RNAs are mostly produced from a large number of protein-coding genes in Arabidopsis.

    abstract::The 5' end of a eukaryotic mRNA transcript generally has a 7-methylguanosine (m7G) cap that protects mRNA from degradation and mediates almost all other aspects of gene expression. Some RNAs in Escherichia coli, yeast, and mammals were recently found to contain an NAD+ cap. Here, we report the development of the metho...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.1903683116

    authors: Zhang H,Zhong H,Zhang S,Shao X,Ni M,Cai Z,Chen X,Xia Y

    更新日期:2019-06-11 00:00:00

  • Role of glycosylation and protein synthesis in insulin receptor metabolism by 3T3-L1 mouse adipocytes.

    abstract::The roles of glycosylation and protein synthesis in the maintenance of insulin receptor levels and turnover rates in 3T3-L1 adipocytes were investigated. The heavy isotope density-shift technique was employed to determine the effects of inhibitors of these processes on the rates of synthesis and degradation of cellula...

    journal_title:Proceedings of the National Academy of Sciences of the United States of America

    pub_type: 杂志文章

    doi:10.1073/pnas.78.5.2908

    authors: Reed BC,Ronnett GV,Lane MD

    更新日期:1981-05-01 00:00:00