Distinguishing surface effects of calcium ion from pore-occupancy effects in Na+ channels.

abstract：:Cognitive flexibility depends on a fast neural learning mechanism for enhancing momentary relevant over irrelevant information. A possible neural mechanism realizing this enhancement uses fast spiking interneurons (FSIs) in the striatum to train striatal projection neurons to gate relevant and suppress distracting cor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Banaie Boroujeni K,Oemisch M,Hassani SA,Womelsdorf T

更新日期：2020-07-28 00:00:00

abstract：:Intermolecular electronic coupling dictates the optical properties of molecular aggregate systems. Of particular interest are photosynthetic pigment-protein complexes that absorb sunlight then efficiently direct energy toward the photosynthetic reaction center. Two-dimensional (2D) ultrafast spectroscopy has been used...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Read EL,Engel GS,Calhoun TR,Mancal T,Ahn TK,Blankenship RE,Fleming GR

更新日期：2007-09-04 00:00:00

abstract：:To identify genetic factors that interact with social environments to impact human health, we used a bioinformatic strategy that couples expression array-based detection of environmentally responsive transcription factors with in silico discovery of regulatory polymorphisms to predict genetic loci that modulate transc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cole SW,Arevalo JM,Takahashi R,Sloan EK,Lutgendorf SK,Sood AK,Sheridan JF,Seeman TE

更新日期：2010-03-23 00:00:00

abstract：:A mouse with a variant hemoglobin was discovered during electrophoretic screening of (C57BL/6J X DBA/2J)F1 progeny of females treated with ethylnitrosourea. The variant trait was transmitted as a simple Mendelian alternate at the Hbb locus in all crosses except those involving the original carrier of the mutation. The...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lewis SE,Johnson FM,Skow LC,Popp D,Barnett LB,Popp RA

更新日期：1985-09-01 00:00:00

abstract：:Motilin, [Met]enkephalin, [Leu]enkephalin, somatostatin, taurine, gamma-aminobutyric acid (GABA), and glycine were tested for their effects on Deiters neurons of the lateral vestibular nucleus in rabbits. Iontophoresis was carried out with multibarrelled micropipettes. All four peptides and three amino acids produced ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chan-Palay V,Ito M,Tongroach P,Sakurai M,Palay S

更新日期：1982-05-01 00:00:00

abstract：:Spinocerebellar ataxia type 7 (SCA7) is characterized by cone-rod dystrophy retinal degeneration and is caused by a polyglutamine [poly(Q)] expansion within ataxin-7, a protein of previously unknown function. Here, we report that ataxin-7 is an integral component of the mammalian STAGA (SPT3-TAF9-ADA-GCN5 acetyltransf...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palhan VB,Chen S,Peng GH,Tjernberg A,Gamper AM,Fan Y,Chait BT,La Spada AR,Roeder RG

更新日期：2005-06-14 00:00:00

abstract：:Messenger RNA from rat liver was partially purified by chromatography on cellulose on the basis of its poly(A) content. Microgram amounts of this RNA stimulate protein synthesis manyfold in a heterologous cell-free system, derived from Krebs ascites cells supplemented with reticulocyte initiation factors. The messenge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schutz G,Beato M,Feigelson P

更新日期：1973-04-01 00:00:00

abstract：:Musical training is known to modify cortical organization. Here, we show that such modifications extend to subcortical sensory structures and generalize to processing of speech. Musicians had earlier and larger brainstem responses than nonmusician controls to both speech and music stimuli presented in auditory and aud...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Musacchia G,Sams M,Skoe E,Kraus N

更新日期：2007-10-02 00:00:00

abstract：:The long-lived ethylene, cyclohexene, and norbornenemercurinium ions prepared in superacidic, low-nucleophilic media have been studied by (13)C and (199)Hg NMR spectroscopy. The norbornenemercurinium ion shows temperature-dependent (13)C and (199)Hg NMR spectra, consistent with equilibration via rapid hydride and Wagn...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olah GA,Garcia-Luna A

更新日期：1980-09-01 00:00:00

abstract：:The effects of NusA on the RNA polymerase contacts made by nucleotides at internal positions in the nascent RNA in Escherichia coli transcription complexes were analyzed by using the photocrosslinking nucleotide analog 5-[(4-azidophenacyl) thio]-UMP. It was placed at nucleotides between +6 and +15 in RNA transcribed f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu K,Hanna MM

更新日期：1995-05-23 00:00:00

abstract：:Intracellular electrophysiological recordings were obtained from a specialized class of "starburst" amacrine cells by using an isolated superperfused retina-eyecup preparation of the rabbit. These cells were injected intracellularly with horseradish peroxidase and identified with light microscopy. A computer-controlle...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Miller RF,Bloomfield SA

更新日期：1983-05-01 00:00:00

abstract：:N-Acetylvalyl-tRNA1Val (AcVal-tRNA1Val) was bound to the P site of uniformly 32P-labeled 70S ribosomes from Escherichia coli and crosslinked to 16S RNA in the 30S ribosomal subunit by irradiation with light of 300-400 nm. To identify the crosslinked nucleotide in 16S RNA. AcVal-tRNA1Val-16S [32P]RNA was digested compl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Prince JB,Taylor BH,Thurlow DL,Ofengand J,Zimmermann RA

更新日期：1982-09-01 00:00:00

abstract：:Natural abundance 13C spin-lattice (T1) relaxation time measurements are reported for unilamellar vesicles of 1,2-dipalmitoylphosphatidylcholine (1,2-dipalmitoyl-sn-glycero-3-phosphocholine), in the liquid crystalline phase, at magnetic field strengths of 1.40, 1.87, 2.35, 4.23, 7.05, 8.45, and 11.7 tesla (resonance f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown MF,Ribeiro AA,Williams GD

更新日期：1983-07-01 00:00:00

abstract：:Cell clones nonproductively transformed by the replication-defective Abelson strain of murine leukemia virus (AbLV) were analyzed for type C viral antigen expression by competition immunoassay. AbLV-transformed mink non-producer lines were found to express a 110,000- to 130,000-molecular weight polyprotein containing ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Reynolds FH Jr,Sacks TL,Deobagkar DN,Stephenson JR

更新日期：1978-08-01 00:00:00

abstract：:The aspartate receptor involved in chemotaxis is modified by methyl esterification at four distinct glutamate residues during the adaptive response of this receptor. To explain the high degree of specificity of this modification, it has been proposed that the methyltransferase recognizes the sequence Glu-Glu-Xaa-Xaa-A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Terwilliger TC,Wang JY,Koshland DE Jr

更新日期：1986-09-01 00:00:00

abstract：:A form of autosomal dominant juvenile myoclonic epilepsy is caused by a nonconservative missense mutation, A322D, in the GABAA receptor alpha1 subunit M3 transmembrane helix. We reported previously that the A322D mutation reduced total and surface alpha1(A322D) subunit protein and that residual alpha1(A322D) subunit r...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gallagher MJ,Ding L,Maheshwari A,Macdonald RL

更新日期：2007-08-07 00:00:00

abstract：:The estrogen receptor-alpha (ERalpha) is a critical transcription factor that regulates epithelial cell proliferation and ductal morphogenesis during postnatal mammary gland development. Tissue recombination and transplantation studies using the first generation of ERalpha knockout (ERKO) mice suggested that this ster...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Feng Y,Manka D,Wagner KU,Khan SA

更新日期：2007-09-11 00:00:00

abstract：:The nucleosomal repeat lengths of bulk chromatin and the chromatin of transcriptionally active and inactive genes were analyzed in two mouse cell lines and adult mouse spleens. The adult beta-globin gene exhibits a nucleosomal repeat length approximately 11 base pairs longer than (i) an inactive embryonic globin gene,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith RD,Seale RL,Yu J

更新日期：1983-09-01 00:00:00

abstract：:The dissociation and association of an immobilized preparation of the octameric enzyme porphobilinogen synthase [5-aminolevulinate hydro-lyase (adding 5-aminolevulinate and cyclizing), EC 4.2.1.24] is described. On treatment of the immobilized preparation with 4 M urea, four subunits per octamer are removed which can ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gurne D,Chen J,Shemin D

更新日期：1977-04-01 00:00:00

abstract：:A survey was performed to estimate the frequency of enterohemorrhagic Escherichia coli O157:H7 or O157:nonmotile (EHEC O157) in feces and on hides within groups of fed cattle from single sources (lots) presented for slaughter at meat processing plants in the Midwestern United States, as well as frequency of carcass co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elder RO,Keen JE,Siragusa GR,Barkocy-Gallagher GA,Koohmaraie M,Laegreid WW

更新日期：2000-03-28 00:00:00

abstract：:S-Adenosylhomocysteine hydrolase (SAHH) is a key enzyme in transmethylation reactions that use S-adenosylmethionine as the methyl donor. Because of the importance of SAHH in a number of S-adenosylmethionine-dependent transmethylation reactions, particularly the 5' capping of mRNA during viral replication, SAHH has bee...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Masuta C,Tanaka H,Uehara K,Kuwata S,Koiwai A,Noma M

更新日期：1995-06-20 00:00:00

abstract：:Synaptobrevin/vesicle-associated membrane protein (VAMP) and syntaxin are potential vesicle donor and target membrane receptors of a docking complex that requires N-ethylmaleimide-sensitive factor (NSF) and soluble NSF-attachment proteins as soluble factors for vesicle fusion with target membranes. Members of this doc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamasaki S,Hu Y,Binz T,Kalkuhl A,Kurazono H,Tamura T,Jahn R,Kandel E,Niemann H

更新日期：1994-05-24 00:00:00

abstract：:In 2009 we reported that depletion of Dicer-1, the enzyme that catalyzes the final step of miRNA biosynthesis, prevents metamorphosis in Blattella germanica. However, the precise regulatory roles of miRNAs in the process have remained elusive. In the present work, we have observed that Dicer-1 depletion results in an ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lozano J,Montañez R,Belles X

更新日期：2015-03-24 00:00:00

abstract：:A 165-kilodalton (kDal) surface glycoprotein encoded by human chromosome 7 (SA-7) had been characterized by using antisera raised against human chromosome 7-containing somatic cell hybrids. We now present evidence that SA-7 is the human receptor for epidermal growth factor (EGF) and that these antisera recognize human...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Carlin CR,Knowles BB

更新日期：1982-08-01 00:00:00

abstract：:The adenoviral E1A oncogene sensitizes mammalian cells to tumor necrosis factor-alpha (TNF-alpha), in part by repressing the nuclear factor-kappa B (NF-kappa B)-dependent defense against this cytokine. Other E1A activities involve binding to either p300/cyclic AMP response element-binding protein (CBP) or retinoblasto...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Cook JL,Walker TA,Worthen GS,Radke JR

更新日期：2002-07-23 00:00:00

abstract：:Glutaredoxins (GRXs), also known as thioltransferases, use glutathione as a cofactor for reduction of disulfides in prokaryotes and eukaryotes. We demonstrate that the vaccinia virus O2L open reading frame encodes a functional GRX, as predicted by Johnson et al. [Johnson, G. P., Goebel, S. J., Perkus, M. E., Davis, S....

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ahn BY,Moss B

更新日期：1992-08-01 00:00:00

abstract：:Site-directed mutagenesis of selected residues of mammalian protein phosphatase 1 (PP-1) has been carried out to further define the mechanism of catalysis, activation by divalent cations, and inhibition by toxins and inhibitory proteins. Mutation of active site residues predicted to bind metals (N124D and H248N) resul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Huang HB,Horiuchi A,Goldberg J,Greengard P,Nairn AC

更新日期：1997-04-15 00:00:00

abstract：:Cyanogen bromide fragments were isolated from the heavy chains of three human IgG myeloma proteins of the V(H)III subgroup, sequenced by an automated method, and localized to the variable region. Inspection of these sequences, together with corresponding stretches from both human and animal proteins (studied in other ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kehoe JM,Capra JD

更新日期：1971-09-01 00:00:00

abstract：:Retroviral entry into cells depends on envelope glycoproteins, whereby receptor binding to the surface-exposed subunit triggers membrane fusion by the transmembrane protein (TM) subunit. We determined the crystal structure at 2.5-A resolution of the ectodomain of gp21, the TM from human T cell leukemia virus type 1. T...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kobe B,Center RJ,Kemp BE,Poumbourios P

更新日期：1999-04-13 00:00:00

abstract：:An inwardly rectifying, ATP-regulated K+ channel with a distinctive molecular architecture, ROMK1, was recently cloned from rat kidney. Using patch clamp techniques, we have investigated the regulation of ROMK1 with particular emphasis on phosphorylation/dephosphorylation processes. Spontaneous channel rundown occurre...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McNicholas CM,Wang W,Ho K,Hebert SC,Giebisch G

更新日期：1994-08-16 00:00:00