Abstract：
:A plasmid that contained the cytomegalovirus (CMV)-promoter-driven lacZ reporter gene (pCMV-lacZ) remained in the epaxial muscle of five of eight goldfish as covalently closed circles, the most functional form of plasmid, for at least 70 days at 22 degrees. It was not present in the gills or elsewhere by polymerase chain reaction and was not integrated. Its expressed protein, Escherichia coli beta-galactosidase (beta-gal), which was in the injected myofibres, was detected in all the fish at 4-21 days and in about half the fish from 28 days until the end of the experiment at 70 days. The numbers of cells that secreted antibody to beta-gal in the kidney peaked at 14 days. Serum antibody and proliferating kidney cells to beta-gal were in all fish from 14 days with a plateau of the responses from 21 days onwards. The plasmid did not induce autoimmune-like antibodies to itself or to single- or double-stranded salmon testis DNA. Plasmids can therefore induce long-term foreign protein expression whilst inducing humoral and cell-mediated immunity without autoimmunity or integration in goldfish.
journal_name
Immunologyjournal_title
Immunologyauthors
Kanellos T,Sylvester ID,Ambali AG,Howard CR,Russell PHdoi
10.1046/j.1365-2567.1999.00688.xkeywords:
subject
Has Abstractpub_date
1999-02-01 00:00:00pages
307-13issue
2eissn
0019-2805issn
1365-2567pii
imm688journal_volume
96pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:In this study we aimed to elucidate the physiological role of gammadelta intraepithelial lymphocytes (IEL) in the mouse intestine. For this purpose, we used T-cell receptor (TCR) Vgamma4/Vdelta5 transgenic mice (KN 6 Tg: BALB/c background, H-2d), and compared the immunological and physiological characteristics of the ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00735.x
更新日期：1999-05-01 00:00:00
abstract：:We quantified immunoglobulin E (IgE) on peritoneal mast cells of interleukin-4 (IL-4)-gene knockout (-/-) mice and wild-type (+/+) controls using a cytofluorometric method, and examined the expression of IgE receptors, estimated by quantifying the total binding of IgE on the mast cells of IL-4 (-/-) mice. The mast cel...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00727.x
更新日期：1999-04-01 00:00:00
abstract：:Interleukin-3 activity is produced by T cells at an early phase after antigenic stimulation, and its quantitation provides a sensitive and non-destructive method for assaying T-cell activation. It can be detected before increased uptake of [3H]thymidine into DNA occurs, and is more sensitive than measuring [3H]thymidi...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
abstract：:C57BL/6 mice were injected with different doses of human T-cell receptor (TCR) V beta 8.1 CDR1 peptide at different times after murine retrovirus (LP-BM5) infection. Injection with TCR V beta 8.1 CDR1 peptide largely prevented the retrovirus-induced reduction in B- and T-cell proliferation, and T-helper 1 (Th1) cytoki...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1996.449551.x
更新日期：1996-02-01 00:00:00
abstract：:Doxorubicin-stimulated whole rat glomeruli and dissociated mesangial and resident glomerular macrophage cells produced the release of interleukin (IL)-1 beta cytokine. This activity increased after the addition of lipopolysaccharide (LPS) or LPS plus indomethacin to the cultures. In the presence of WEB2086 [platelet-a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Studies have demonstrated that the anti-tumour effect of natural killer (NK) cells is successful for patients with several cancers. Although interleukin-32 (IL-32) is endogenously expressed in NK cells, cytolytic function of NK cells against cancer cells has not been fully demonstrated. In the present study, we found ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2011.03513.x
更新日期：2012-01-01 00:00:00
abstract：:In the present study we have investigated whether bovine erythrocytes (Eb) specifically sensitized with human polyclonal IgA1 (Eb-IgA1) are able to bind to resident adherent rat peritoneal cells (PM phi). Rat PM phi formed rosettes with Eb-IgA1 at room temperature and at 37 degrees. The formation of these rosettes cou...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-06-01 00:00:00
abstract：:LacZ (Gal)-reactive immune cells were transferred into athymic nu/nu mice inoculated with Gal-expressing syngeneic tumour cells (ESbL-Gal) in order to study tumour-protective T-cell memory. This transfer prevented tumour outgrowth in recipients and resulted in the persistence of a high frequency of Gal-specific CD8(+)...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2005.02163.x
更新日期：2005-07-01 00:00:00
abstract：:MRL/Mp-lpr/lpr (lpr/lpr) mice but not the congenic MRL/Mp-+/+ (+/+) mice, develop a generalized lymph node (LN) hypertrophy reflecting the expansion of a T-cell population that acts as an enhancing factor for autoimmunity. In order to characterize better this T-cell population, we investigated some of its surface prop...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:The aim of this study was to examine the role of T cells on the alteration of terminal sugars of goblet cell mucins in the small intestinal mucosa of parasitized rats and to clarify the biological significance of the altered mucins in the mucosal defence against intestinal helminths. For this purpose, Nippostrongylus ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Norovirus (NoV) -derived virus-like particles (VLPs) resemble empty shells of the virus and NoV P-particles contain only protruding domains of the NoV capsid. Both NoV-derived subviral particles show similar functionality and antigenicity in vitro and are considered to be potential vaccine candidates against NoV gastr...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2011.03516.x
更新日期：2012-01-01 00:00:00
abstract：:Ribonucleic acid (RNA) extracts were obtained from lymph nodes of guinea-pigs that had previously been immunized with a purified testicular antigen emulsified in Freund's complete adjuvant. The RNA extracts were incubated with normal guinea-pig peritoneal exudate cells (NGP-PEC). After treatment, the NGP-PEC cells sho...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:Tumour-associated macrophages (TAMs) represent a predominant population of inflammatory cells that present in solid tumours. TAMs are mostly characterized as alternatively activated M2-like macrophages and are known to orchestrate nearly all stages of tumour progression. Experimental investigations indicate that TAMs ...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/imm.12023
更新日期：2013-02-01 00:00:00
abstract：:We have previously reported that the immunization of BALB/c mice with MOPC-104E myeloma protein (M104E) induced idiotype-specific Ly 1-negative B lymphocytes that had an ability to enhance idiotype-positive anti-dextran antibody production. It was also shown that the cell interaction between idiotypic and anti-idiotyp...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:Natural killer (NK) cells, which can exert early and powerful anti-tumour and anti-viral responses, are important components of the innate immune system. DNAX accessory molecule-1 (DNAM-1) is an activating receptor molecule expressed on the surface of NK cells. Recent findings suggest that DNAM-1 is a critical regulat...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/imm.12516
更新日期：2015-11-01 00:00:00
abstract：:The effect of disodium cromoglycate (DSCG) upon human immunoglobulin (Ig) isotypes and IgG subclasses production by purified B cells was studied. DSCG enhanced IgM, IgG1, IgG2, IgG3, IgG4 and IgA production in a dose-dependent fashion, while DSCG failed to induce IgE production at any concentrations tested by purified...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Leukosialin or CD43 is a heavily O-glycosylated transmembrane protein expressed on all cells of the haematopoietic cell lineage with the exception of red blood cells and mature B cells. This antigen has been identified in human, mouse and rat with monoclonal antibodies. Although orthologues of many human and rodent le...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00604.x
更新日期：1999-01-01 00:00:00
abstract：:Acquisition of T-cell central tolerance involves distinct pathways of self-antigen presentation to thymocytes. One pathway termed indirect presentation requires a self-antigen transfer step from thymic epithelial cells (TECs) to bone marrow-derived cells before the self-antigen is presented to thymocytes. The role of ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12904
更新日期：2018-07-01 00:00:00
abstract：:Polyclonal rabbit anti-idiotypic (Ab2) antibodies raised against the antiprogesterone mAb DB3 (Ab1) were used to induce an Ab3 antiprogesterone response in BALB/c mice. While the affinity of Ab3 sera for progesterone was 10-50-times lower than that of DB3, their steroid-binding specificity showed considerable similari...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00006.x
更新日期：2000-06-01 00:00:00
abstract：:To analyse the immunological mechanism of hepatocellular injury in hepatitis B virus (HBV) infection, the immunoreactivity of HBV-encoded antigens as a target for cytotoxic T lymphocyte (CTL) response was examined using recombinant vaccinia virus (RVV) expressing surface protein (S), precore/core protein (PC), and cor...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-10-01 00:00:00
abstract：:Tick larvae (Boophilus microplus) were extracted, fractionated by gel filtration and ion-exchange chromatography, and the fractions assayed by enzyme-linked immunosorbent assay (ELISA) against sera from cattle immunized and protected by antigens from the mid-gut of adult ticks. Unfractionated, whole larval extracts an...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-07-01 00:00:00
abstract：:This study addresses the question of whether human peripheral CD4+ CD45RA+ T cells possess antigen-specific immune memory. CD4+ CD45RA+ T cells were isolated by a combination of positive and negative selection. Putative CD4+ CD45RA+ cells expressed CD45RA (98.9%) and contained < 0.1% CD4+ CD45RO+ and < 0.5% CD4+ CD45R...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00274.x
更新日期：1997-07-01 00:00:00
abstract：:High-density human B lymphocytes were prepared from tonsillar mononuclear cells by depletion of adherent cells and T lymphocytes, followed by discontinuous density gradient centrifugation. The B cells were analysed for the levels of expression of the CD25 (Tac) antigen marker by flow cytometry following culture with a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-01-01 00:00:00
abstract：:Monoclonal antibodies to human complement component C9 were used to localise the membrane attack complex (MAC) of human complement on muscle fibres from patients with autoimmune myositis. A pigeon erythrocyte 'model' system was used to screen the available monoclonal antibodies for their ability to bind C9 after its i...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:The aim of this study is to investigate the clinical significance of the ratio between interleukin-17 (IL-17) secreting cell and FOXP3-positive regulatory T cell (FOXP3(+) Treg) infiltration in renal allograft tissues with acute T-cell-mediated rejection (ATCMR). Fifty-six patients with biopsy-proven ATCMR were includ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2012.03588.x
更新日期：2012-07-01 00:00:00
abstract：:Functional studies on heteroantisera raised against mouse B cells in rabbits suggest a correlation between thymus-independent B cells and LPS-responsive B cells, and differences in either from thymus-dependent B cells. Furthermore it seems possible to follow B-lymphocyte differentiation from antigen-uncommitted precur...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:In lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice, alveolar macrophages (AMphi) produce much more tumour necrosis factor-alpha than peritoneal macrophages (PMphi) when stimulated with LPS (10 microgram/ml), but the induction of inducible nitric oxide synthase (iNOS) gene expression and production of nitric oxide...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00908.x
更新日期：1999-12-01 00:00:00
abstract：:The number, distribution and surface phenotype of dividing cells in the thymus, and differences between the cell cycle status of thymocyte subpopulations, were studied in fetal and post-natal lambs using double-labelling techniques. Dividing cells were labelled in vivo for various periods with 5-bromo-2-deoxyuridine (...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-11-01 00:00:00
abstract：:Vitamin A and its active metabolite retinoic acid are essential for the development and function of many tissues including the immune system. The induction of mucosal homing receptors on T and B cells by mucosal dendritic cells (DC) depends on the presence of vitamin A. Recent studies indicate that also the differenti...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/imm.12228
更新日期：2014-05-01 00:00:00
abstract：:Interleukin-19 (IL-19) plays an important role in asthma by stimulating T helper type 2 (Th2) cytokine production. Interestingly, IL-4, a key Th2 cytokine, in turn up-regulates IL-19 expression in bronchial epithelial cells, so forming a positive feedback loop. In atopic dermatitis (AD), another Th2 disease closely re...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12339
更新日期：2014-12-01 00:00:00
