Abstract：
:High-density human B lymphocytes were prepared from tonsillar mononuclear cells by depletion of adherent cells and T lymphocytes, followed by discontinuous density gradient centrifugation. The B cells were analysed for the levels of expression of the CD25 (Tac) antigen marker by flow cytometry following culture with a variety of cytokines. IL-4 could induce elevated levels of CD25 on high-density, putatively resting B lymphocytes in a dose-dependent fashion. Expression of CD25 at the B-cell surface could not be promoted by interleukin-2 (IL-2), interferon-gamma (IFN-gamma) or by a crude preparation of B-cell growth factor 2 (BCGF-2). Mitogenic challenge of the B cells with pokeweed mitogen (PWM) and a combination of phorbol ester and calcium ionophore were similarly ineffective, although a small increase in CD25 expression could be detected when the B cells were cultured with phytohaemagglutinin A (PHA). The ability of IL-4 to promote CD25 expression was abolished by the presence of IFN-gamma in the culture. Titration experiments suggested that the amount of IL-4 required to produce a half-maximal increase in CD25 expression was approximately 40 U/ml; this is considerably greater than the 8-10 U/ml required to produce the equivalent effect on CD23 expression. The ability of IL-4 to promote CD25 expression in the high-density B-lymphocyte population was apparently independent of proliferation of the cells. IL-4 could not promote Tac expression on high-density T cells prepared from the same tissue source.
journal_name
Immunologyjournal_title
Immunologyauthors
Butcher RD,McGarvie GM,Cushley Wsubject
Has Abstractpub_date
1990-01-01 00:00:00pages
57-64issue
1eissn
0019-2805issn
1365-2567journal_volume
69pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:Dendritic cells (DC) are the initiators of immune responses and are present in most tissues in vivo. To generate myeloid DC from monocytes (MoDC) in vitro the necessary cytokines are granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Using degenerated primers delineated from other spec...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2005.02319.x
更新日期：2006-04-01 00:00:00
abstract：:We have analysed the heavy and light chain variable region genes of two monoclonal antibodies, specific for the Sm antigen (RSP1; IgG kappa) and for cardiolipin (RSP4; IgM lambda), derived from a patient with active systemic lupus erythematosus (SLE). We have established that the variable region genes of the RSP1 auto...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:We have previously demonstrated that immunoglobulin A (IgA)(-/-) knockout (KO) mice exhibit levels of susceptibility to influenza virus infection that are similar to those of their normal IgA(+/+) littermates. To understand the mechanism of this apparent mucosal immunity without IgA, immunoglobulin isotype and T helpe...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.0019-2805.2001.01368.x
更新日期：2002-03-01 00:00:00
abstract：:CD4 and CD8 T-cell clones were generated using the Mx9 monoclonal antibody (mAb), which recognizes the V beta 8 T-cell receptor (TcR) gene family. The interaction of these clones with the Mx9 antibody was analysed and all were found to be specifically stimulated to proliferate by plastic-adherent Mx9. In the presence ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-07-01 00:00:00
abstract：:An investigation was made of the properties of cytotoxic T cells induced by Con A and exposure to LCM virus-infected cells. As a basis for such studies, the optimal conditions for in vitro Con A stimulation of in vivo LCM virus-primed C3H mouse splenocytes were determined. The most potent cytotoxicity was obtained whe...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:The ultimate outcome of T-cell recognition of peptide-major histocompatibility complex (MHC) complexes is determined by the molecular context in which antigen presentation is provided. The paradigm is that, after exposure to peptides presented by steady-state dendritic cells (DCs), inhibitory signals dominate, leading...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2009.03216.x
更新日期：2010-05-01 00:00:00
abstract：:Veiled cells (VC), isolated from the thoracic duct of irradiated lymphadenectomized mice (MLNX) or peritoneal macrophages (PM phi were incubated with isotope-labelled hen egg lysozyme (HEL), purified protein derivative (PPD) or keyhole limpet haemocyanin (KLH) in vitro. About 2-10 times less antigen was associated wit...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-11-01 00:00:00
abstract：:The effects of cyclophosphamide (CY) on antigenic competition in IgE antibody production were studied in mice treated with the drug on different days and immunized with a mixture of two non-related antigens. Injection of 100 mg of CY/kg of body weight 3 days before or 6 days after immunization resulted in a partial or...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:The class distribution of 4193 monoclonal gammopathies (MG), detected over approximately 10 years in western France (WF), was compared to that established by a compilation of twenty-seven series from the literature. Several points emerged from this study: the incidence of the light chain MG (4.8%) was slightly lower t...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1985-11-01 00:00:00
abstract：:Peripheral blood mononuclear cells (PBMC) from a patient suffering from the hyper IgE syndrome were used to generate phytohaemagglutinin (PHA)-expanded T-cell clones (all CD4+, CD8-, CD23-). A selection of the clones was tested for their ability to help IgE secretion by culturing with normal B cells in the presence of...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-05-01 00:00:00
abstract：:The activation of guinea-pig eosinophils was studied by measuring the production of superoxide anion (O2-) and the secretion of eosinophil peroxidase (EPO). Phorbol myristate acetate (PMA), calcium ionophore, plasma-activated zymosan, concanavalin A and recombinant human anaphylatoxin C5a induced the release of varyin...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-04-01 00:00:00
abstract：:Immunoglobulin G4 (IgG4) antibodies have been known for some time to be functionally monovalent. Recently, the structural basis for this monovalency has been elucidated: the in vivo exchange of IgG half-molecules (one H-plus one L-chain) among IgG4. This process results in bispecific antibodies that in most situations...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1046/j.0019-2805.2001.01341.x
更新日期：2002-01-01 00:00:00
abstract：:Functional studies on heteroantisera raised against mouse B cells in rabbits suggest a correlation between thymus-independent B cells and LPS-responsive B cells, and differences in either from thymus-dependent B cells. Furthermore it seems possible to follow B-lymphocyte differentiation from antigen-uncommitted precur...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:Following prescapular lymphadenectomy, the cellular composition of afferent (peripheral) lymph from eight out of 13 calves in which the afferent and efferent lymphatic ducts had anastomosed was examined using monoclonal antibodies (mAb) specific for bovine lymphoid populations. Afferent lymph leucocytes (ALL) consiste...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1987-10-01 00:00:00
abstract：:Chlamydia trachomatis is a major cause of sexually transmitted disease, infertility and reactive arthritis in the Western world, and of trachoma in the developing world. There is evidence that the chronic inflammatory reaction seen in diseases associated with chlamydiae represents a delayed-type hypersensitivity respo...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:The haemolymph of the colonial ascidian Botrylloides leachii contains two haemagglutinins which have been termed HA-1 and HA-2 respectively. The HA-1 agglutinin is specific for guinea-pig erythrocytes, requires Ca2+ ions for its activity and has an apparent mol. wt. of about 200,000. It can be adsorbed by guinea-pig b...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:Haptenated murine Langerhans' cells (LCs) have been reported to induce contact sensitivity when injected via the subcutaneous, intraperitoneal and, in some instances, the intravenous route. Similar studies were undertaken to elucidate the role of the LC in the induction of contact sensitivity in the guinea-pig. The su...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1987-12-01 00:00:00
abstract：:The immunosuppressive activity of serum from PVG rats following orthotopic transplantation of DA liver has been examined in vitro. Liver grafts in this combination are never rejected, but induce a state of specific transplantation tolerance in the recipient. Serum from such tolerant animals was able to inhibit prolife...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1987-08-01 00:00:00
abstract：:To assess the role of CD5 and CD28 in the pathogenesis of the decreased cellular immune function in patients with rheumatoid arthritis (RA) we analysed the expression and function of these T-cell surface molecules. The expression of CD5 as well as of CD28 in synovial and peripheral blood T cells was similar to that of...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:In order to analyse the molecular mechanism of lymphocyte activation defect in MRL/MP-lpr (MRL/1) mice, c-myc proto-oncogene expression was examined in MRL/1 lymph node cells stimulated by various agents. Since a transient increase of c-myc RNA in early hours is required for lymphocyte activation, detection of c-myc m...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:The cellular basis of the mechanism of immunological tolerance to human gamma-globulin (H gamma G) induced in foetal and neonatal mice by materno-foetal or materno-neonatal transfer after a single injection of tolerogen (deaggregated H gamma G) into the mothers was investigated using a cell transfer system and assays ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-05-01 00:00:00
abstract：:In many types of tumours, especially pancreatic adenocarcinoma, miR-301a is over-expressed. This over-expression results in negative regulation of the target gene of miR-301a, the nuclear factor-κB (NF-κB) repressing factor (NKRF), increasing the activation of NF-κB and production of NF-κB-responsive pro-inflammatory ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12139
更新日期：2013-11-01 00:00:00
abstract：:Culture dishes precoated with thin layers of acid soluble rat tail collagen simplify conditions necessary to obtain in vitro high IgG anti-DNP responses from primed and boosted mice. In contrast to Mishell-Dutton type cultures the use of collagen precludes the need to continually rock cultures and to add nutrients. Re...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-06-01 00:00:00
abstract：:Eosinophil recruitment to the airways, including involvement of haemopoietic eosinophil-basophil progenitors (Eo/B-CFU), is primarily regulated by interleukin-5 (IL-5) and eotaxin. In this study, we investigated the haemopoietic mechanisms in upper and lower airway eosinophilic inflammation. Ovalbumin (OVA) sensitized...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2005.02109.x
更新日期：2005-03-01 00:00:00
abstract：:Endothelial cell activation antigens may play important roles in immune responses and in inflammation. This report describes the identification and characterization of a monoclonal antibody, named EAA-B, which reacts specifically with human umbilical vein endothelial (HUVE) cells pre-treated with tumour necrosis facto...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1992-08-01 00:00:00
abstract：:The discovery that corticosteroids can alter leucocyte distribution implies that suppression of PFC in the spleen is not necessarily indicative of suppression of the antibody response as a whole. Therefore we have examined IgM and IgG serum antibodies and PFC in the thymus, spleen, femoral marrow, popliteal, thoracic ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:The role of genetic factors associated with the immunoglobulin heavy chain locus (Igh) in the development of benign monoclonal gammapathy (BMG), a benign B-cell proliferative disorder, was investigated in six Igh congenic mouse strains during ageing. The strains used had a C57BL or BALB background: C57BL/6, BALB.Igb a...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:Mucosal immunization offers the potential for inducing IgA antibody responses in the vagina, the site of infection for many viruses, including herpes simplex type 2 (HSV-2). To investigate this possibility, mice were immunized intranasally with 10 micrograms glycoprotein D2 (gD2) from HSV combined with a series of adj...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1998.00441.x
更新日期：1998-04-01 00:00:00
abstract：:The ability to expand tumour-infiltrating lymphocytes in vitro has been greatly enhanced by the use of antigen-independent mechanisms of immune cell costimulation. We have produced human, using the K562 cell line, and murine, using YAC-1 cells, artificial antigen presenting cells (aAPC) and demonstrate that these cell...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2004.01853.x
更新日期：2004-05-01 00:00:00
abstract：:Interleukin-2 (IL-2) is a mainstay for current immunotherapeutic protocols but its usefulness in patients is reduced by severe toxicities and because IL-2 facilitates regulatory T (Treg) cell development. IL-21 is a type I cytokine acting as a potent T-cell co-mitogen but less efficient than IL-2 in sustaining T-cell ...
journal_title：Immunology
pub_type： 临床试验,杂志文章
doi：10.1111/imm.12061
更新日期：2013-05-01 00:00:00
