Abstract:
:In many types of tumours, especially pancreatic adenocarcinoma, miR-301a is over-expressed. This over-expression results in negative regulation of the target gene of miR-301a, the nuclear factor-κB (NF-κB) repressing factor (NKRF), increasing the activation of NF-κB and production of NF-κB-responsive pro-inflammatory cytokines such as interleukin-8, interferon-β, nitric oxide synthase 2A and cytochrome oxidase subunit 2 (COX-2). However, in immune cells, mechanisms that regulate miR-301a have not been reported. Similar to tumour cells, Toll-like receptor (TLR) -activated macrophages produce NF-κB-responsive pro-inflammatory cytokines. Therefore, it is of considerable interest to determine whether miR-301a regulates the secretion of cytokines by immune cells. In the present study, we demonstrate that the expression of miR-301a was decreased in TLR-triggered macrophages. Through targeting NKRF, miR-301a affected the activity of NF-κB and the expression of pro-inflammatory genes downstream of NF-κB such as COX-2, prostaglandin E2 and interleukin-6. In addition, when lipopolysaccharide-treated macrophages were simultaneously stimulated with trichostatin A, an inhibitor of histone deacetylases, the expression of miR-301a increased, whereas NKRF and pro-inflammatory cytokine expression decreased. However, further investigation revealed that there was no correlation between the induction of miR-301a and the inhibitory effect of trichostatin A on lipopolysaccharide-induced gene expression in macrophages. In summary, our study indicates a new mechanism by which miR-301a regulates inflammatory cytokine expression in macrophages, which may clarify the regulatory role of microRNAs in immune-mediated inflammatory responses.
journal_name
Immunologyjournal_title
Immunologyauthors
Huang L,Liu Y,Wang L,Chen R,Ge W,Lin Z,Zhang Y,Liu S,Shan Y,Lin Q,Jiang Mdoi
10.1111/imm.12139subject
Has Abstractpub_date
2013-11-01 00:00:00pages
314-22issue
3eissn
0019-2805issn
1365-2567journal_volume
140pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Splenic immune responses having varying dependence on accessory cell co-operation have been studied after infection of mice with Friend virus. Infection has no effect on cell proliferation or antibody production in cultures stimulated with E. coli lipopolysaccharide. The response in vivo to type III pneumococcal polys...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1978-04-01 00:00:00
abstract::Experiments were designed to determine the in vivo cell-cycle phase of lymphocytes in secondary lymphoid follicles, and whether B-cell proliferation and apoptosis occur within follicular dendritic cell (FDC)-associated clusters. Using frozen serial sections of human tonsils, lymphoid follicles were stained to reveal h...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00203.x
更新日期:1997-04-01 00:00:00
abstract::The in vitro migration activity of monocytic cells and the effect of lymphocytes in the delayed hypersensitivity (DH) reaction induced by intrapleural injection of PPD into FCA-sensitized guinea-pigs was investigated. The in vitro migration of exudate mononuclear cells was lower than that of comparable blood cells, an...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-04-01 00:00:00
abstract::The role of Peyer's patches in the local intestinal and serum antibody responses to keyhole limpet haemocyanin (KLH) was studied in rabbits with chronically isolated ileal loops. Four weekly doses of 400 microgram KLH were administered into loops prepared with and without Peyer's patches. Isotype-specific IgA and IgG ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1981-03-01 00:00:00
abstract::Experiments were conducted to test the hypothesis that opsonic and non-opsonic phagocytic capacities are differentially regulated by resting and wound-derived macrophages. Furthermore, the phagocytosis of non-opsonized zymosan and beta-glucan particles was quantified to determine whether cells differentially regulate ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2001.01291.x
更新日期:2001-10-01 00:00:00
abstract::Selective Alzheimer disease indicator-1 (seladin-1) is a broadly expressed oxidoreductase and is related to Alzheimer disease, cholesterol metabolism and carcinogenesis. The effect of lipopolysaccharide (LPS) on the expression of seladin-1 was examined using RAW 264.7 macrophage-like cells and murine peritoneal macrop...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03274.x
更新日期:2010-09-01 00:00:00
abstract::HIV infection is associated with increased representation of T cells bearing an activated, memory (CD45RO+) phenotype. Although administration of antiretroviral agents and interleukin-2 (IL-2) augment depleted CD4+ T-cell numbers, such therapies have been preferentially beneficial for CD45RO+ T cells. Interleukin-15 (...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00807.x
更新日期:1999-06-01 00:00:00
abstract::Previous studies have shown that the degradation of soluble immune complexes or aggregates (AIgG) by normal peritoneal macrophages can be enhanced by complement. The enhancement of degradation was shown to be at least in part dependent on the number of C3b molecules bound per complex. The present investigations indica...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::The sensitivity of host (circulating) forms of T. cruzi to cell-mediated immunological destruction and requirements for the reaction were examined. Both human and mouse leucocytes were found to kill significant numbers of parasites in the presence of specific antibodies against the flagellates. Antibody involvement wa...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Peroxisome proliferator activated receptor-gamma (PPARgamma) has been reported to exert anti-inflammatory properties in endotoxic shock and sepsis. One phenomenon that alters the inflammatory response to endotoxin [lipopolysaccharide (LPS)] is endotoxin tolerance, which is caused by previous exposure to endotoxin. Her...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2007.02734.x
更新日期:2008-05-01 00:00:00
abstract::The investigation of the role of the macrophage in cutaneous leishmaniasis has been prompted by observations of the clinical behaviour of the infection. In contrast to the self-healing oriental sore, chronic leishmaniasis is characterized by persistent lesions and leishmania recidiva by lesions that flare up locally l...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-10-01 00:00:00
abstract::The CD4-specific monoclonal antibody (mAb) CC26, when tested on a total of 143 cattle, failed to react with T cells from 16% of animals and gave reduced intensity staining in a further 35% of animals. The results of family studies with groups of half- and full-siblings indicated that CC26 recognizes an allele of CD4 w...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-12-01 00:00:00
abstract::We examined the mechanisms of disturbed T-lymphocyte function that occurred during Schistosoma japonicum infection of BALB/c mice from the viewpoint of the interleukin cascade reaction. Each point of the interleukin cascade reaction was examined. First, IL-1 production by adherent peritoneal or spleen cells, as a sour...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1987-10-01 00:00:00
abstract::The endogenous cannabinoid system plays an important role in regulating the immune system. Modulation of endogenous cannabinoids represents an attractive alternative for the treatment of inflammatory disorders. This study investigated the effects of URB597, a selective inhibitor of fatty acid amide hydrolase (FAAH), t...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02838.x
更新日期:2008-10-01 00:00:00
abstract::T helper type 17 (Th17) cells and regulatory T (Treg) cells are two distinct T-cell subsets with opposite effects on immune functions. While Th17 cells are a key effector in the immune response and play critical roles in the development of autoimmunity and inflammation, Treg cells orchestrate the overall immune respon...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12994
更新日期:2018-12-01 00:00:00
abstract::In lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice, alveolar macrophages (AMphi) produce much more tumour necrosis factor-alpha than peritoneal macrophages (PMphi) when stimulated with LPS (10 microgram/ml), but the induction of inducible nitric oxide synthase (iNOS) gene expression and production of nitric oxide...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00908.x
更新日期:1999-12-01 00:00:00
abstract::Spleen lymphocytes from C4-deficient (C4D) and Albany strains of guinea-pigs, 1-7 days, 3-6 and 12-16 months old, genetically related to inbred strains 13 and 2 respectively, were analysed in terms of their expression of cell surface markers, allogenic and T- and B-cell mitogenic responses, and interleukin-1 (IL-1) an...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1992-10-01 00:00:00
abstract::Two phenotypically distinct subpopulations of dendritic cells (SIRPalpha+ CC81Ag- DC and SIRPalpha- CC81Ag+ DC) have previously been identified in bovine afferent lymph which show functional differences when assayed in vitro. The purpose of this study was to investigate whether differences in cytokine production betwe...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01712.x
更新日期:2003-09-01 00:00:00
abstract::We have examined the distribution of the complement (C) regulatory proteins CD59, membrane cofactor protein (MCP) and decay-accelerating factor (DAF) on mature sperm and compared expression of these proteins in parallel both during spermatogenesis and in the prostate. Enhanced immunoperoxidase staining and radioimmuno...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::Chlamydiae are a major cause of infertility and preventable blindness and there is currently no effective vaccine in humans or rodents against these organisms. We have previously shown that a peptide of 12 amino acids (termed TINKP) from a conserved region of the major outer membrane protein (MOMP) of Chlamydia tracho...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::BALB/c mice develop myasthenic symptoms after immunization with rodent acetylcholine receptor (AChR). After immunization with Torpedo AChR (TAChR), their CD4+ cells become strongly sensitized against a conserved region of the TAChR alpha subunit sequence (residues alpha 304-322), and cross-react vigorously with the ho...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-05-01 00:00:00
abstract::Recent studies employing reverse transription-polymerase chain reaction (RT-PCR) have demonstrated the intrathymic presence of mRNA for various autoantigens, including thyroglobulin (Tg). Deliberations on the mechanisms of central tolerance usually assume that this approach detects intact mRNA transcripts that can be ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2005.02119.x
更新日期:2005-05-01 00:00:00
abstract::Adoptive transfer of T-cells is a promising therapy for many cancers. To enhance tumour recognition by T-cells, chimeric antigen receptors (CARs) consisting of signalling domains fused to receptors that recognize tumour-associated antigens can be expressed in T-cells. While CAR T-cells have shown clinical success for ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/imm.13187
更新日期:2020-07-01 00:00:00
abstract::The role of the monocyte in human cytomegalovirus (HCMV)-induced immunosuppression was examined by assessing the ability of the virus to directly suppress various monocyte accessory cell functions. Both patient-derived and laboratory-adapted strains of HCMV were capable of impairing antigen-presenting functions of pur...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-02-01 00:00:00
abstract::A positive association has been observed in Igh allotype "congenic' mice between skin graft rejection and a block to effective T--B co-operation in the development of both IgM and IgG responses to sheep erythrocytes. Mismatch of Igh alone is insufficient for such a block to become apparent since pairs of Igh-allotype ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::Peripheral blood mononuclear (PBM) cells from five of 21 patients with common variable immunodeficiency (CVI) stimulated in vitro by antigen produced antibodies normally. In 11 of the 16 non-responders, removal of adherent suppressor cells by Sephadex G-10 or removal of monocytes or OKT8 positive suppressor lymphocyte...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::Non-T cells from tonsil or blood were fractionated according to buoyant density, isotype of surface immunoglobulin, or the ability to form rosettes with mouse erythrocytes. Each fraction was tested for the ability to proliferate in response to B. catarrhalis (Bc) and, for comparison, Staphylococcus aureus Cowan 1 (SAC...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::Chronic antigenic stimulation leads to gradual accumulation of late-differentiated, antigen-specific, oligoclonal T cells, particularly within the CD8(+) T-cell compartment. They are characterized by critically shortened telomeres, loss of CD28 and/or gain of CD57 expression and are defined as either CD8(+) CD28(-) or...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2567.2011.03470.x
更新日期:2011-09-01 00:00:00
abstract::The linker protein LAT is expressed mainly in T and natural killer (NK) cells. LAT-deficient mice have an arrest of intrathymic T-cell development at the CD4+ CD8+ stage and lack mature T cells in the periphery. However, no gross abnormality in development and function of the B and NK cells has been described. Here we...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2003.01671.x
更新日期:2003-07-01 00:00:00
abstract::CD4 and CD8 T-cell clones were generated using the Mx9 monoclonal antibody (mAb), which recognizes the V beta 8 T-cell receptor (TcR) gene family. The interaction of these clones with the Mx9 antibody was analysed and all were found to be specifically stimulated to proliferate by plastic-adherent Mx9. In the presence ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00