Abstract：
:Culture dishes precoated with thin layers of acid soluble rat tail collagen simplify conditions necessary to obtain in vitro high IgG anti-DNP responses from primed and boosted mice. In contrast to Mishell-Dutton type cultures the use of collagen precludes the need to continually rock cultures and to add nutrients. Reduction of the cell density in collagen dishes to 10(6) cells/ml is without detriment to the antibody response. Cells adhering to collagen coated dishes after 24 h incubation provide an enriched population of IgG anti-DNP precursor cells; which will mature to antibody secretion at very low cell density (10(4) -10(5) cells/ml). However, as changes in the adhesive properties of cells to collagen occur during cell maturation, attempts to adapt the technique to obtain clones of antibody secreting cells were not wholly successful.
journal_name
Immunologyjournal_title
Immunologyauthors
Kemshead JT,Askonas BAsubject
Has Abstractpub_date
1978-06-01 00:00:00pages
1071-6issue
6eissn
0019-2805issn
1365-2567journal_volume
34pub_type
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