Abstract：
:Antibody plaque-forming cells (FC) to the somatic antigens of Vibrio cholerae were enumerated in the spleen of mice after primary and secondary immunization with a heat-killed vaccine prepared from the vibrios. Immunocytes releasing both high efficiency IgM and low efficiency IgG antibody were readily detected using a direct and facilitated plaque procedure in agar gel. Whereas the peak numbers of IgM-PFC after primary immunization occurred on days 12 to 14, the peak IgG-PFC response developed somewhat later (16-18 days). After a second injection of vaccine larger numbers of both IgM- and IgG-PFE appeared in the mouse spleens, with peak responses for both occurring between days 5 and 8. The largest number of IgG-PFC developed in spleens of mice given a second injection of vaccine 6-8 weeks after primary immunization. The dose of killed vibrios used for priming markedly affected both the magnitude and the class of antibody-forming cells appearing during the secondary response; 1--10 mug vaccine was more effective than higher or lower doses for priming the mice to a heightened secondary response. Furthermore, the antigenic specificity of both the IgM- and IgG-PFC appearing after secondary immunization was directly related to the strain of cholera bacilli used for priming. When mice were immunized with the Ogawa strains of cholera most of the secondary PFC after booster immunization with the serologically distinct Inaba strain was directed towards the common antigen shared by both strains and not to the type specific antigen of the Inaba vibrios. The specificity of the anti-vibrio PFC during both the primary and secondary responses was readily demonstrable by inhibition experiments using sonicated or soluble cholera antigens. Prior incubation of these antigens with test spleen cells in the agar gel effictively inhibited development of the vibriolytic plaques, regardless of antibody class. Similar antigen extracts from toher bacteria had no effect. The immunoglobulin nature of the plaques was also demonstrable by inhibition with low dilutions of rabbit anti-mouse globulin serum incorporated into the agar plates prior to testing; both IgM and IgG plaues were inhibited.
journal_name
Immunologyjournal_title
Immunologyauthors
Friedman Hsubject
Has Abstractpub_date
1975-08-01 00:00:00pages
283-99issue
2eissn
0019-2805issn
1365-2567journal_volume
29pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:We have shown that human endothelial cells (EC) are protected against complement-mediated injury by the inducible expression of decay-accelerating factor (DAF). To understand further the importance of DAF regulation, we characterized EC DAF expression on murine EC in vitro and in vivo using a model of glomerulonephrit...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2003.01733.x
更新日期：2003-10-01 00:00:00
abstract：:When cultured on a collagen matrix at a density of 7 X 10(4) cells/cm2 for 48 hr, 80 +/- 10% of unfractionated and 70 +/- 14% of T-enriched human blood lymphocytes from eight healthy individuals developed a motile morphology defined as the presence of lamellar surface activity and cytoplasmic flattening. During cultur...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:We describe the characterization of a novel HLA Class I molecule, which we have isolated from chorionic cytotrophoblast cell membranes, and from a trophoblast-derived choriocarcinoma cell line, BeWo; classical HLA Class I antigens are not expressed on these cells. This antigen is an electrophoretically non-polymorphic...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1986-12-01 00:00:00
abstract：:We have established lines and clones of helper T cells specific for the antigen fowl gamma globulin (FGG) and maintained these cells in continuous proliferation in vitro for up to 8 months. During this period the specific helper activity was assessed in vitro in T cell-dependent anti-hapten responses. These pure popul...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-07-01 00:00:00
abstract：:Cellular adhesion molecules play a central role in leucocyte migration through peripheral blood and tissues. A crucial stage in these events in selectin-mediated adhesion involving E-selectin expressed on activated endothelium interacting with a range of carbohydrate ligands expressed by specific subpopulations of leu...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:It has previously been demonstrated that a gene on chromosome 1 in or near Mls-1 controls, on the surface of B cells, the mobility and aggregability of major histocompatibility complex (MHC) class II molecules but not the mobility or aggregability of other B-cell molecules, such as immunoglobulin (Ig) and class I anti...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-12-01 00:00:00
abstract：:Antigenic encounter by T cells induces immunological synapse formation and T-cell activation. Using different concentrations of toxic shock syndrome toxin-1 (TSST-1) as stimulus, we examined the capacities of dendritic cells (DC) and macrophages (Mphi) to prime syngeneic naive T cells. DCs were, under all experimental...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2003.01758.x
更新日期：2003-12-01 00:00:00
abstract：:The CD40 ligand (gp39) is transiently expressed on activated CD4+ T cells and mediates cognate helper function by interacting with CD40 on B cells. Increasing evidence suggests, however, critical involvement of gp39 not only in antibody-mediated responses but also in the development of effector T cells. Here, we have ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.1997.00421.x
更新日期：1997-03-01 00:00:00
abstract：:Previously, a putative immunosuppressant-coding gene was identified from a complementary DNA library derived from the salivary glands of partially-fed Haemaphysalis longicornis. Using real-time polymerase chain reaction, the gene was shown to be predominantly expressed during blood feeding with the site of expression ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2008.02890.x
更新日期：2009-02-01 00:00:00
abstract：:Cytotoxic T-lymphocytes (CTL) play an important role in the control of human immunodeficiency virus (HIV) and of human cytomegalovirus (HCMV) infection. Following highly active antiretroviral therapy (HAART), most studies have demonstrated a decline in the frequency of HIV-specific CTL. We analysed the effect of HAART...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2006.02334.x
更新日期：2006-05-01 00:00:00
abstract：:Osteopontin (OPN) is important for the function of fibroblasts, macrophages and lymphocytes during inflammation and wound healing. In recent studies of experimental colitis we demonstrated exacerbated tissue destruction in OPN-null mice, associated with reduced tumour necrosis factor-alpha expression and increased mye...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02682.x
更新日期：2007-12-01 00:00:00
abstract：:Anti-proteinase 3 antibodies are implicated in the pathogenesis of small vessel vasculitis. These are primarily immunoglobulin G (IgG), with different subclasses predominating at different stages of disease. However, little is known of their respective roles in pathogenesis. We have previously shown that patient IgG4 ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2009.03108.x
更新日期：2009-10-01 00:00:00
abstract：:In this report the antigen and restriction specificity of human T-cell clones induced with Dermatophagoides farinae (D. farinae) and isolated from an atopic individual with perennial rhinitis has been investigated. Of the six clones analysed, four were species specific and two showed cross-reactivity for the closely r...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-08-01 00:00:00
abstract：:Mouse lymph node cells cultured with Concanavalin A (Con A) in serum-free medium containing 59Fe-transferrin took up 59Fe more rapidly than cells cultured without Con-A. Uptake of iron commenced rapidly and preceded the onset of DNA synthesis in stimulated cells. Total uptake of transferrin during culture was much low...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-06-01 00:00:00
abstract：:We have previously reported that the immunization of BALB/c mice with MOPC-104E myeloma protein (M104E) induced idiotype-specific Ly 1-negative B lymphocytes that had an ability to enhance idiotype-positive anti-dextran antibody production. It was also shown that the cell interaction between idiotypic and anti-idiotyp...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-04-01 00:00:00
abstract：:Newborn mammals are highly susceptible to respiratory infections. Although maternal antibodies (MatAb) offer them some protection, they may also interfere with their systemic immune response to vaccination. However, the impact of MatAb on the neonatal mucosal immune response remains incompletely described. This study ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/imm.12222
更新日期：2014-04-01 00:00:00
abstract：:We investigated the individual CD8+ populations with natural killer (NK) cell markers (NK-type T cell); CD56 single positive (CD56)-T cells, CD56/CD57 double positive (DP)-T cells and CD57 single positive (CD57)-T cells in the peripheral blood. All NK-type T-cell populations expressed CD122 and intermediate levels of ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2003.01575.x
更新日期：2003-02-01 00:00:00
abstract：:with AgB-primed B or T cells and injected into syngeneic X-irradiated recipients. Anti-THF and anti-Eid purified by an AgB-specific T suppressor factor (TSF) affi-gel adsorbent retain their ability to specifically initiate [3H]-thymidine incorporation of AgB-primed T cells. The data indicate that both anti-THF and ant...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-02-01 00:00:00
abstract：:This study examines the expression of mRNA for the Th2 cytokine, interleukin-4 (IL-4). Peripheral blood mononuclear cells from deer infected with Mycobacterium bovis or vaccinated with live or killed M. bovis bacillus Calmette-Guérin (BCG) were cultured with mycobacterial antigens. IL-4 mRNA production was assayed usi...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.1996.tb00014.x
更新日期：1996-06-01 00:00:00
abstract：:Membrane attack complexes of complement occur around thyroid follicles in Graves' disease and Hashimoto's thyroiditis. The lytic potential of such complexes is controlled by membrane-bound and fluid phase regulators and we have investigated the role of these in autoimmune thyroid disease. By immunohistochemical staini...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1994-04-01 00:00:00
abstract：:The haemolymph of the colonial ascidian Botrylloides leachii contains two haemagglutinins which have been termed HA-1 and HA-2 respectively. The HA-1 agglutinin is specific for guinea-pig erythrocytes, requires Ca2+ ions for its activity and has an apparent mol. wt. of about 200,000. It can be adsorbed by guinea-pig b...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-02-01 00:00:00
abstract：:We found in previous experiments that fractionation of non-immune mouse spleen cells on bovine serum albumin density gradients yields two subpopulations of T cells, one of high, the other of low density. Both subopopulations could be stimulated in corporate thymidine by the T cell-specific mitogen concanavalin A (con ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1975-03-01 00:00:00
abstract：:Accumulating evidence indicates that B cells may undergo sequential rearrangements at the light chain loci, despite already expressing light chain receptors. This phenomenon may occur in the bone marrow and, perhaps, in germinal centers. As immunoglobulin (Ig)kappa light chains usually rearrange before Iglambda light ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2004.01902.x
更新日期：2004-07-01 00:00:00
abstract：:The phospholipid platelet-activating factor 1-0-hexadecyl-2-acetyl-SN-glycero-3-phosphorylcholine (AGEPC) initiates platelet function by interacting specifically with 1399 +/- 498 (mean +/- SD) high-affinity membrane receptors per platelet. In studies designated to characterize the high affinity binding site, AGEPC-hu...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-05-01 00:00:00
abstract：:The innate immune response provides the initial defence mechanism against infection by other organisms. However, an excessive immune response will cause damage to host tissues. In an attempt to identify microRNAs (miRNAs) that regulate the innate immune response in inflammation and homeostasis, we examined the differe...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2010.03374.x
更新日期：2011-05-01 00:00:00
abstract：:Regeneration of the thymus was studied in rats that were treated with a single oral dose of the organotin compound di-n-butyltin dichloride (DBTC). After an initial maximum depletion of cortical BrdU+ thymocytes on day 2 after treatment, repopulation appeared to start on day 3 as indicated by an increased number of Br...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-04-01 00:00:00
abstract：:Pregnancy in sheep is associated with changes in numbers of specific T-lymphocyte populations in the uterine endometrium. These changes probably contribute to evasion by the conceptus of maternal immunological rejection and indicate a possible role for T cells in placental growth, parturition and post-parturient uteri...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2001.01182.x
更新日期：2001-03-01 00:00:00
abstract：:Specific pathogen-free B6D2 mice were infected intravenously with 10(8) viable BCG, M. habana or M. simiae and the level of tuberculin hypersensitivity to 2.5 micrograms PPD or cytoplasmic protein antigens (CPA) prepared from the other organisms was determined using the footpad swelling test with increasing time after...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-03-01 00:00:00
abstract：:Studies in long-term non-progressors (LTNP) have suggested that the quality of the CD8(+) response may involve protective human leucocyte antigen (HLA) class I alleles. However, studies examining the expansion ability of different functional CD8(+) T cells and their association with HLA class I alleles are lacking. LT...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2011.03490.x
更新日期：2011-11-01 00:00:00
abstract：:Tuberculin-active peptide-reactive T-cell lines were established from the popliteal lymph node of BALB/cA mice immunized with heat-killed Mycobacterium tuberculosis to investigate the cellular mechanisms in the protective immunity of tuberculosis. These T-cell lines, consisting mainly of L3T4 surface antigen-positive ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-10-01 00:00:00
