Abstract：
:The non-obese diabetic (NOD) mouse is an animal model for insulin-dependent diabetes that has many similarities to the human disease. NOD mice transgenic for the Ea gene, allowing expression of the E molecule, are protected from diabetes and rarely develop insulitis. An Ea transgene mutated in the promoter region, (DeltaY) lacks E expression on most B cells, thymic medullary epithelium and primary antigen-presenting cells, and confers no protection whatsoever. We have used these transgenic NOD mice, together with non-transgenic NOD mice, to study the correlation of E expression and production of interleukin-4 (IL-4) and interferon-gamma (IFN-gamma). We show that protected E-transgenic NOD mice have elevated levels of IL-4 compared with non-transgenic mice, both in the thymus and in the periphery. However, susceptible DeltaY-transgenic mice have elevated thymic IL-4 levels, but express almost as little IL-4 as non-transgenic NOD mice in the periphery. This drop in peripheral IL-4 production seen in DeltaY-transgenic mice thus correlates with the decreased E expression in the periphery of DeltaY-transgenic NOD mice. In contrast, there were no differences in IFN-gamma production between the three NOD lines. We suggest that Ea-transgenic NOD mice have E-selected regulatory T cells producing IL-4, which are subsequently activated by E-expressing primary antigen-presenting cells in the periphery. This activation would then be instrumental for the E-mediated protection from disease in NOD mice. Such a process would explain the total absence of protection in DeltaY-transgenic NOD mice, despite their widespread E expression.
journal_name
Immunologyjournal_title
Immunologyauthors
Brenden N,Böhme Jdoi
10.1046/j.1365-2567.1998.00580.xsubject
Has Abstractpub_date
1998-09-01 00:00:00pages
1-7issue
1eissn
0019-2805issn
1365-2567journal_volume
95pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract：:All but one of nine Acholeplasma strains were susceptible to killing by gnotobiotic-calf serum while all but one of fifteen Mycoplasma strains were resistant. The mechanism of killing was examined using inhibitors of complement, viz. EDTA. MgEGTA. epsilon-amino caproic acid, and also by desalting serum and adding MgCl...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-11-01 00:00:00
abstract：:Secretory component (SC or polymeric immunoglobulin receptor) on mucosal epithelial cells mediates transcytosis of polymeric immunoglobulin into external fluids and functions as a receptor for polymeric immunoglobulin. SC expression in a human colonic adenocarcinoma cell line, HT-29 has been reported to be up-regulate...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1997.00341.x
更新日期：1997-10-01 00:00:00
abstract：:Bisphenol A (BPA) and p-nonylphenol (NP) are representative endocrine disruptors (EDs) that may have adverse effects on human health. The influence of these compounds on allergic immune responses remains unclear. In this study, we have examined the effects of BPA and NP on production of interleukin-4 (IL-4), a pro-inf...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2003.01631.x
更新日期：2003-05-01 00:00:00
abstract：:The discovery that corticosteroids can alter leucocyte distribution implies that suppression of PFC in the spleen is not necessarily indicative of suppression of the antibody response as a whole. Therefore we have examined IgM and IgG serum antibodies and PFC in the thymus, spleen, femoral marrow, popliteal, thoracic ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1979-10-01 00:00:00
abstract：:Defective regulation of secondary immunoglobulin V(D)J gene rearrangement promotes the production of autoantibodies in systemic lupus erythematosus (SLE). It remains unclear, however, whether the regulation of the recombination-activating genes RAG1 and RAG2 is effective in SLE. RAG1 and RAG2 messenger RNA expression ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02649.x
更新日期：2007-11-01 00:00:00
abstract：:Isolated human mononuclear cells were fractionated according to their membrane characteristics or physical properties. Adherent cells were depleted by filtration through glass columns; phagocytic cells were removed by iron treatment and cell subpopulations capable of forming rosettes with sheep erythrocytes (E), eryth...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1976-03-01 00:00:00
abstract：:We report the cloning of cDNA encoding the pig homologue of human integrin-associated protein (IAP or CD47). A pig CD47-specific probe was generated by polymerase chain reaction (PCR) amplification of pig leucocyte cDNA, using primers based on consensus regions among the known sequences of CD47 from different species....
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2002.01465.x
更新日期：2002-08-01 00:00:00
abstract：:Enriched CD4+CD8-/CD4-CD8-, CD4-CD8+/CD4-CD8- and CD4-CD8- cell suspensions were prepared from normal peripheral blood by selective immunomagnetic depletion of monoclonal antibody-defined lymphocyte populations. Subsequent examination of these modified cell fractions by two-colour flow cytometry provided a means of de...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1990-07-01 00:00:00
abstract：:Previous studies have shown that no cell-mediated immunity against LCM virus-infected cells can be detected in neonatally established LCM virus carrier mice suggesting that they are immunologically tolerant to virally-altered cell membrane antigens. In this communication experiments are described aimed at analyzing th...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1978-02-01 00:00:00
abstract：:Despite the capacity for antigen-specific activation and rapid clonal expansion, homeostatic mechanisms ensure that the mature immune system contains a relatively stable number of T cells. In recent years, it has become apparent that this stability is a consequence of apoptotic death of most of the specific T cells ge...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2000.00048.x
更新日期：2000-07-01 00:00:00
abstract：:Hyper-immunoglobulin M (IgM) syndrome (HIGM) is a rare primary immunodeficiency characterized by elevated or normal IgM and absent or markedly decreased amounts of IgG, IgA and IgE. The X-linked form (HIGM1) is the most common type and is caused by mutations in the gene for CD40L, a T-cell surface molecule required fo...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2006.02520.x
更新日期：2007-04-01 00:00:00
abstract：:The Fas/Fas-ligand (FasL) system plays an important role in regulation of apoptosis and the immune response, and is exploited by mycobacteria to evade the immune response. This study was performed to investigate the distribution and levels of FasL and Fas in lymph node granulomas and sera of tuberculous lymphadenitis ...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2004.02080.x
更新日期：2005-02-01 00:00:00
abstract：:CD45 is known to regulate signalling through many different surface receptors in diverse haemopoietic cell types. Here we report for the first time that CD45-/- bone marrow dendritic cells (BMDC) are more activated than CD45+/+ cells and that tumour necrosis factor (TNF) and interleukin-6 (IL-6) production by BMDC and...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2006.02363.x
更新日期：2006-06-01 00:00:00
abstract：:Epidermal keratinocytes are induced to express MHC class II molecules in a variety of disease states associated with immune activity. To investigate the mechanism of this process we have exposed murine and rat keratinocytes to a variety of lymphokines and monitored changes in their MHC molecule expression. Murine cult...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1989-01-01 00:00:00
abstract：:The fluorescence-activated cell sorter and a rosette-depletion technique were used to separate rat spleen lymphocytes and BCG-activated lymphocytes into subpopulations with and without the antigens defined by W3/13, W3/23 and OX8 monoclonal antibodies. The resultant populations were then tested for natural killer (NK)...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-01-01 00:00:00
abstract：:The influence of pooled human IgG preparations for intravenous use (i.v.Ig) on in vitro-induced cytokine production was studied at the single-cell level using cytokine-specific monoclonal antibodies (mAb) and indirect immunofluorescent technique. Cultured mononuclear cells from peripheral blood from healthy adult dono...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-06-01 00:00:00
abstract：:Different genetic mutations have been described in complement component C7 deficiency, a molecular defect clinically associated with an increased susceptibility to neisserial recurrent infections. In this work we report the genetic basis of C7 deficiency in two different Spanish families (family 1 and family 2). In fa...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2004.01997.x
更新日期：2004-12-01 00:00:00
abstract：:A soluble form of Fc epsilon RII/CD23 is spontaneously released from most lymphoblastoid cell lines established by the Epstein-Barr virus (EBV). Such a product was purified on an IgE-Sepharose column and its pyrogenic effect was investigated in rabbits. This preparation induced a monophasic fever in rabbits, with a pe...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-08-01 00:00:00
abstract：:The suppression of immune responsiveness to heterologous antigenic stimulation during concurrent infection with Nematospiroides dubius was reproduced using soluble antigens derived from adult parasites. Immunosuppression appeared to be selective in that the administration of equivalent quantities of an irrelevant hete...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-04-01 00:00:00
abstract：:Male lymphocytic choriomeningitis (LCM) virus-carrier mice were mated to uninfected females and their offsprings tested for (i) LCMV virus and (ii) their potential to generate LCM virus-specific cytotoxic T cells. Among about 500 offspring tested no example of antigen-independent inheritance of tolerance to LCM virus ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1984-06-01 00:00:00
abstract：:Some plasmacytomas produce myeloma proteins with known antibody specificities and the secretion of these proteins by individual tumour cells can be determined using haemolytic plaque assay. After a 3 day culture of mouse plasmacytoma cells in medium containing 10% normal mouse serum, the number of plaques was reduced ...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1980-10-01 00:00:00
abstract：:Among their diverse roles as transcriptional regulators during development and cell fate specification, the RUNX transcription factors are best known for the parts they play in haematopoiesis. RUNX proteins are expressed throughout all haematopoietic lineages, being necessary for the emergence of the first haematopoie...
journal_title：Immunology
pub_type： 杂志文章,评审
doi：10.1111/imm.12535
更新日期：2015-12-01 00:00:00
abstract：:The effects of cyclophosphamide (CY) on antigenic competition in IgE antibody production were studied in mice treated with the drug on different days and immunized with a mixture of two non-related antigens. Injection of 100 mg of CY/kg of body weight 3 days before or 6 days after immunization resulted in a partial or...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1982-09-01 00:00:00
abstract：:T lymphocytes and macrophages (M phi) have been seen to accumulate at sites of lesions in blood vessel walls, suggesting that these cells may contribute to the pathogenesis of inflammatory reactions. Tumour necrosis factor-alpha (TNF-alpha), a cytokine produced by both M phi and T lymphocytes, plays a major role in in...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:A critical role for leukotriene B(4) (LTB(4)) and/or platelet-activating factor (PAF) in regulating polymorphonuclear cell (PMN) trafficking to inflammatory sites has been reported in a number of experimental inflammatory models. In vitro, newly synthesized LTB(4) and PAF were shown to act in an autocrine/paracrine or...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2567.2007.02767.x
更新日期：2008-07-01 00:00:00
abstract：:In addition to macromolecular interactions that provide co-stimulation during antigen-presenting cell (APC) and CD4+ T-cell conjugation, covalent chemical events between specialized ligands have been implicated in T-cell co-stimulation. These take the form of transient Schiff base formation between carbonyls and amine...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.2001.01290.x
更新日期：2001-09-01 00:00:00
abstract：:Bovine Concanavalin A-induced suppressor cells were generated from lymphocytes which were non-adherent to anti-immunoglobulin coated dishes and cells possessing receptors for peanut agglutinin. Bovine lymphocytes, preincubated with 25 microgram/ml of Con A for 40-45 hr, could suppress the responses of autologous cells...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
abstract：:The role of endogenously mediated fever and exogenous hyperthermia as modulators of immune functions remains poorly understood. It is known that fever is mediated by several cytokines, including interleukin-1 alpha and interleukin-1 beta (IL-1 alpha and IL-1 beta), interleukin-6 (IL-6), tumour necrosis factor-alpha (T...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1991-07-01 00:00:00
abstract：:The effect on murine immunoglobulin G (IgG) glycosylation of altering IgG production in vivo was assessed in interleukin (IL)-6 transgenic and CD4 knockout mice. C57BL/6 mice carrying the IL-6 transgene showed increased levels of circulating IgG. This was associated with decreased levels of galactose on the IgG oligos...
journal_title：Immunology
pub_type： 杂志文章
doi：10.1046/j.1365-2567.1999.00896.x
更新日期：1999-11-01 00:00:00
abstract：:Antibody-dependent cellular cytotoxicity (ADCC) and Fc receptor-mediated phagocytosis were determined with human monocytes, monocyte-derived macrophages and activated macrophages, using rabbit IgG-covered sheep red blood cells (EAs) and anti-D-treated human erythrocytes (EAhu) as target cells. Monocyte and macrophage-...
journal_title：Immunology
pub_type： 杂志文章
doi：
更新日期：1988-03-01 00:00:00
