Abstract:
:Mucosa-associated invariant T (MAIT) cells express the semi-invariant T-cell receptor TRAV1-2 and detect a range of bacteria and fungi through the MHC-like molecule MR1. However, knowledge of the function and phenotype of bacteria-reactive MR1-restricted TRAV1-2(+) MAIT cells from human blood is limited. We broadly characterized the function of MR1-restricted MAIT cells in response to bacteria-infected targets and defined a phenotypic panel to identify these cells in the circulation. We demonstrated that bacteria-reactive MR1-restricted T cells shared effector functions of cytolytic effector CD8(+) T cells. By analysing an extensive panel of phenotypic markers, we determined that CD26 and CD161 were most strongly associated with these T cells. Using FACS to sort phenotypically defined CD8(+) subsets we demonstrated that high expression of CD26 on CD8(+) TRAV1-2(+) cells identified with high specificity and sensitivity, bacteria-reactive MR1-restricted T cells from human blood. CD161(hi) was also specific for but lacked sensitivity in identifying all bacteria-reactive MR1-restricted T cells, some of which were CD161(dim) . Using cell surface expression of CD8, TRAV1-2, and CD26(hi) in the absence of stimulation we confirm that bacteria-reactive T cells are lacking in the blood of individuals with active tuberculosis and are restored in the blood of individuals undergoing treatment for tuberculosis.
journal_name
Immunologyjournal_title
Immunologyauthors
Sharma PK,Wong EB,Napier RJ,Bishai WR,Ndung'u T,Kasprowicz VO,Lewinsohn DA,Lewinsohn DM,Gold MCdoi
10.1111/imm.12461subject
Has Abstractpub_date
2015-07-01 00:00:00pages
443-53issue
3eissn
0019-2805issn
1365-2567journal_volume
145pub_type
杂志文章相关文献
IMMUNOLOGY文献大全abstract::Immunoglobulin E is quantitatively a minor immunoglobulin class in serum, but nevertheless the major class of antibody mediating type I hypersensitivity reactions and hence, type I allergic phenomena. The bovine epsilon gene is one of the as yet uncharacterized mammalian immunoglobulin genes. We have therefore cloned ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00352.x
更新日期:1997-11-01 00:00:00
abstract::The expanded CD8+ T-lymphocyte population arising in response to viral infection controls the virus but could also prove damaging to the host unless safely removed at the end of the immune response. Apoptosis provides a mechanism whereby this can be achieved, as apoptotic cells are recognized and engulfed by macrophag...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::We have previously shown that chlorpromazine (CPZ) inhibits tumour necrosis factor (TNF) production and protects against endotoxic shock in mice. In this paper we investigated the effect of pretreatment with CPZ, 4 mg/kg i.p. 30 min before, compared with dexamethasone (DEX; 3 mg/kg) on the induction of other endotoxin...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-06-01 00:00:00
abstract::Oral administration of antigen induces a state of tolerance that is associated with activation of CD8+ T cells that can transfer unresponsiveness to naïve syngeneic hosts. These T cells are not lytic, but they inhibit development of antibody, CD4+ T helper cell, and CD8+ cytotoxic T lymphocyte (CTL) responses upon ado...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.0019-2805.2003.01793.x
更新日期:2004-02-01 00:00:00
abstract::The non-specific normal immunosuppressive protein (Nip) has been described in our laboratory and its biological activity was extensively studied. In the present study, further purification analyses of Nip were conducted. Fractionation of Nip by Ultrogel AcA 34 column resulted in peak (I) that displayed Nip activity in...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::The role of gut-derived CD4+ T cells in clearance of Pseudomonas aeruginosa from the lung was studied by cell transfer experiments. Mesenteric lymph node cells from unimmunized rats, or rats orally immunized with either killed P. aeruginosa or Helicobacter pylori, were transferred to naive rats which were subsequently...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::Passive transfer of immunity to Taenia taeniaeformis was achieved with serum taken 14, 21, 49 and 63 days after infection. The protective capacity of serum collected at 14 and 21 days resided in the 7Sgamma2 immunoglobulins and appeared to be partics the infection progressed the range of chromatographic fractions show...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-11-01 00:00:00
abstract::Natural killer (NK) cells have a specialized function in peripheral organs, which is determined by the organ-specific niches. We have attempted to explore whether lung NK cells display a particular phenotype according to their function in the unique pulmonary environment in health or during respiratory infection in mi...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2012.03607.x
更新日期:2012-09-01 00:00:00
abstract::It has been suggested that tumour cell lysis by gamma-radiation induces a tumoral antigen release eliciting an immune response. It is not clear how a specific immune response in cervical cancer patients is developed after radiotherapy. This study is an attempt to investigate the role of the human papillomavirus type 1...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2008.02912.x
更新日期:2009-04-01 00:00:00
abstract::Extracorporeal photochemotherapy (ECP) has been shown to be a potent activator of peripheral blood macrophages because it causes a marked release of macrophage-dependent proinflammatory cytokines, and it is therefore currently considered to be a safe and non-toxic immunomodulatory treatment. On this basis we studied t...
journal_title:Immunology
pub_type: 临床试验,杂志文章
doi:10.1046/j.1365-2567.1997.00325.x
更新日期:1997-09-01 00:00:00
abstract::Isolated normal human peripheral neutrophils became cytotoxic to chicken red blood cells (CRBC) in the presence of phytohaemagglutinin (PHA) and concanavalin A (Con A), a phenomenon which we have termed lectin-dependent neutrophilmediated cytotoxicity (LDNMC). Substantial cytotoxicity could be demonstrated by 1 h of i...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::The distribution of T lymphocytes expressing the different polypeptides of the leucocyte common antigen (LCA) family detected by CD45R and UCHL1 antibodies has been studied in normal lymphoid tissues. In the thymus most cortical thymocytes express UCHL1 and co-express CD4 and CD8. The more mature membrane CD3+ (mainly...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1989-04-01 00:00:00
abstract::Invariant natural killer T (iNKT) cells are an innate population of T cells identified by the expression of an invariant T-cell receptor and reactivity to lipid-based antigens complexed with CD1d. They account for a small percentage of lymphocytes, but are extremely potent and play central roles in immunity to infecti...
journal_title:Immunology
pub_type: 杂志文章,评审
doi:10.1111/imm.12481
更新日期:2015-08-01 00:00:00
abstract::Recent studies involving T cells that express gamma delta T-cell receptor (gamma delta TcR) have raised the possibility that Qa-1/Tla region class I major histocompatibility complex (MHC)-like molecules are antigen-presenting molecules for gamma delta TcR. In this report, cytotoxic T lymphocyte (CTL) clones specific f...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-12-01 00:00:00
abstract::Antibody-dependent cellular cytotoxicity (ADCC) and Fc receptor-mediated phagocytosis were determined with human monocytes, monocyte-derived macrophages and activated macrophages, using rabbit IgG-covered sheep red blood cells (EAs) and anti-D-treated human erythrocytes (EAhu) as target cells. Monocyte and macrophage-...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-03-01 00:00:00
abstract::We have previously reported that placental macrophages of fetal origin have a decreased ability to present antigen. To clarify the underlying mechanism for this deficiency, we have generated primary fetal macrophage cell lines. Our data show that despite their defective antigen-presenting ability, fetal macrophages do...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.00369.x
更新日期:1997-12-01 00:00:00
abstract::Several cloned, non-leukaemic mouse haemopoietic cell lines are believed to be uniquely dependent on interleukin-3 (IL-3) for their survival. All of these lines require the addition of WEHI-3B cell-conditioned medium for their growth. We have examined the growth requirements of four IL-3-dependent cell lines and found...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Mutation of the interleukin-2 (IL-2) receptor gamma chain, which also serves as a component of the receptor complexes for IL-4, 7, 9 and 15, results in severe immune deficiency. We hypothesized that the immunological immaturity of healthy neonates might be associated with low levels of expression of this receptor mole...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::We previously reported that FTY720 is an efficient inducer of apoptosis in lymphocytes and cultured cell lines. In the present study, HL-60 human promyerocytoma cells also induced apoptosis through in vitro treatment with the drug, demonstrating extensive DNA fragmentation 6 hr after incubation. The major target of FT...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1997.d01-2281.x
更新日期:1997-08-01 00:00:00
abstract::In mice, the plasma cell (PC) niche in the bone marrow is close to the haematopoietic stem cell (HSC) niche. We investigated whether PCs can be mobilized into the peripheral blood (PB) in healthy donors receiving granulocyte colony-stimulating factor (G-CSF) for the induction of HSC mobilization into the PB. G-CSF inc...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2010.03361.x
更新日期:2011-02-01 00:00:00
abstract::Specific IgE and IgG responses to highly purified Ambrosia (ragweed) allergens, Amb a V, Amb t V and Amb p V from the artemisiifolia (short), trifida (giant) and psilostachya (western) species are strongly associated with HLA-DR2 and Dw2 (DR2.2) in allergic Caucasoid individuals. To investigate the molecular basis of ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1991-07-01 00:00:00
abstract::The interleukin-2 receptor alpha chain (IL-2R alpha) is a T-cell growth factor receptor and is known to be induced in helper T cells by infection with human T-cell leukaemia virus type-1 (HTLV-1). The Rex protein of HTLV-1 has been shown to stabilize IL-2R alpha mRNA. Although the 3' untranslated region of many RNA ha...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1995-12-01 00:00:00
abstract::We previously described the processing of antibodies to CD74 (the major histocompatibility complex class II-associated invariant chain, Ii), by B-cell lymphoma cell lines. These cells expressed relatively low levels of Ii on the surface, but the molecules were rapidly internalized and replaced by new molecules, so tha...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1999.00868.x
更新日期:1999-10-01 00:00:00
abstract::A positive association has been observed in Igh allotype "congenic' mice between skin graft rejection and a block to effective T--B co-operation in the development of both IgM and IgG responses to sheep erythrocytes. Mismatch of Igh alone is insufficient for such a block to become apparent since pairs of Igh-allotype ...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::The effect of stem cell factor (SCF) on histamine and tumour necrosis factor-alpha (TNF-alpha) release from rat peritoneal mast cells (PMC) was determined and the intracellular pathways involved in the potentiation of histamine secretion were investigated. The effects of SCF (2-100 ng/ml) were examined following both ...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.1996.d01-733.x
更新日期:1996-10-01 00:00:00
abstract::This study investigates the effect of feeding fermented milks with Lactobacillus casei, Lactobacillus acidophilus and a mixture of both micro-organisms on the specific and non-specific host defence mechanisms in Swiss mice. Animals fed with fermented milk for 8 days (100 micrograms/day) showed an increase in both phag...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1988-01-01 00:00:00
abstract::The recovery of the B-cell population in adult thymectomized, irradiated and bone marrow-reconstituted mice (T X BM mice was estimated at various times after bone marrow transplantation. The spleen cells to be tested were mixed with dexamethasone-resistant thymocytes (DRT) and sheep red blood cells (SRBC) and transfer...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::Chicken antibodies (immunoglobulin Y; IgY) to the alpha Gal epitope (galactose alpha-1,3-galactose) bind to alpha Gal antigens of mouse and porcine tissues and endothelial cells in vitro and block human anti-alpha Gal antibody binding, complement activation and antibody-dependent cell-mediated lysis mechanisms. The ac...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2567.2000.00136.x
更新日期:2000-12-01 00:00:00
abstract::Interleukin-1 (IL-1) is an important mediator of inflammation and has been implicated in several forms of immunopathology. Here we have investigated whether IL-1 plays a role in the enteropathy which occurs during a graft-versus-host reaction (GVHR) in mice. Non-irradiated (CBA x BALB/c) F1 mice with GVHR had increase...
journal_title:Immunology
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::Human papillomaviruses (HPVs) are obligate epithelial pathogens and typically cause localized mucosal infections. We therefore hypothesized that T-cell responses to HPV antigens would be greater at sites of pathology than in the blood. Focusing on HPV-16 because of its association with cervical cancer, the magnitude o...
journal_title:Immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2567.2006.02465.x
更新日期:2006-12-01 00:00:00